BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,190	A→G	T93A (ACT→GCT)	yrbI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,190	0	A	G	99.6%	730.9 / NA	231	T93A (ACT→GCT)	yrbI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (104/126); minor base T (0/1); total (104/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340100‑3340279

  ttGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTGAACTGGTAGAAGATCGTTGTGGCACATTGGGGGtcgctca                                                                                       >  1:4181892/1‑93 (MQ=255)

   tgtgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctcac                                                                                      <  1:4311719/93‑1 (MQ=255)

    gtgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATcgctcact                                                                                     >  1:2264276/1‑93 (MQ=255)

     tgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACtt                                                                                    <  2:3277569/93‑1 (MQ=255)

      gcgcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTg                                                                                   <  1:3892664/93‑1 (MQ=255)

        gcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTa                                                                                 <  2:3831655/93‑1 (MQ=255)

         cTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAt                                                                                <  1:652984/93‑1 (MQ=255)

          tCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATc                                                                               <  1:1392063/93‑1 (MQ=255)

          tCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATc                                                                               >  1:2043082/1‑93 (MQ=255)

           cACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCa                                                                              <  1:4473906/93‑1 (MQ=255)

            aCCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGCATCAg                                                                             >  1:1061451/1‑93 (MQ=255)

             ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            >  1:479910/1‑93 (MQ=255)

             ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            >  1:1793693/1‑93 (MQ=255)

              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           <  2:710164/93‑1 (MQ=255)

              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           <  1:703344/93‑1 (MQ=255)

              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           >  2:1531165/1‑93 (MQ=255)

              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           <  2:2520330/93‑1 (MQ=255)

               tctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          >  1:135734/1‑93 (MQ=255)

                 tGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCa                                                                        <  2:4210997/93‑1 (MQ=255)

                 tGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCa                                                                        >  1:2720939/1‑93 (MQ=255)

                 tGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCa                                                                        >  2:1372233/1‑93 (MQ=255)

                   atatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGt                                                                      >  2:2145557/1‑93 (MQ=255)

                    tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTc                                                                     >  2:3522532/1‑93 (MQ=255)

                       tGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCATGGGCAGTcaaa                                                                  <  2:1453391/93‑1 (MQ=255)

                        gAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaac                                                                 <  2:3406531/93‑1 (MQ=255)

                         aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                <  2:1489469/93‑1 (MQ=255)

                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             <  2:4080859/93‑1 (MQ=255)

                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             <  2:2754495/93‑1 (MQ=255)

                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             <  2:3094417/93‑1 (MQ=255)

                             cGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACt                                                            <  1:1853367/93‑1 (MQ=255)

                              gCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGTGGATCGCTCAATTGTATCAGGGGCAGTCAAACAAACTg                                                           >  1:3964980/1‑93 (MQ=255)

                               cTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGa                                                          >  2:2223840/1‑93 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATTGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  2:196536/93‑1 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGac                                                         <  2:543468/93‑2 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:955219/93‑1 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:2195565/93‑1 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         >  1:4116235/1‑93 (MQ=255)

                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        <  2:3311728/93‑1 (MQ=255)

                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        <  1:3398800/93‑1 (MQ=255)

                                   cATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGc                                                      <  1:3338385/93‑1 (MQ=255)

                                    aTTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGcc                                                     <  2:1650500/93‑1 (MQ=255)

                                        ccGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTa                                                 <  2:904383/93‑1 (MQ=255)

                                         cGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAg                                                >  1:1827074/1‑93 (MQ=255)

                                         cGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAg                                                <  2:2349099/93‑1 (MQ=255)

                                           ggCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCg                                              >  1:3005478/1‑93 (MQ=255)

                                            gCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGa                                             >  2:2812073/1‑93 (MQ=255)

                                               aaaGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATct                                          <  2:3481020/93‑1 (MQ=255)

                                               aaaGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATct                                          <  1:1941202/93‑1 (MQ=255)

                                                aaGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctg                                         >  1:2331698/1‑93 (MQ=255)

                                                 aGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgc                                        <  1:2789423/93‑1 (MQ=255)

                                                 aGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgc                                        <  1:191845/93‑1 (MQ=255)

                                                 aGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgc                                        >  2:4375093/1‑93 (MQ=255)

                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       >  1:1527637/1‑93 (MQ=255)

                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:1508896/93‑1 (MQ=255)

                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:434590/93‑1 (MQ=255)

                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgcc                                       <  1:4422037/93‑2 (MQ=255)

                                                    cgaaaCTGGTAGAAGAGCGTGTAACCACATTGGGCATCGCTCACTTGTATCAGGGTCAGTCAAACAACCTGATCTGCTTTAGGTATCTGCTgg                                     <  2:1601796/91‑1 (MQ=255)

                                                    cTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTgg                                     <  1:594610/93‑1 (MQ=255)

                                                    cTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTgg                                     >  1:2900242/1‑93 (MQ=255)

                                                    cTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTgg                                     >  1:303308/1‑93 (MQ=255)

                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaa                                   <  2:4194307/93‑1 (MQ=255)

                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaa                                   >  1:922413/1‑93 (MQ=255)

                                                       aaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaa                                  >  2:4513558/1‑93 (MQ=255)

                                                        aCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaaa                                 >  2:461449/1‑93 (MQ=255)

                                                         cTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaaaa                                >  1:3716190/1‑93 (MQ=255)

                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               >  2:3055032/1‑93 (MQ=255)

                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              <  2:1238754/93‑1 (MQ=255)

                                                             tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  1:3645162/93‑1 (MQ=255)

                                                             tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  2:2706906/93‑1 (MQ=255)

                                                             tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  1:3874754/93‑1 (MQ=255)

                                                             tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            >  1:3478236/1‑93 (MQ=255)

                                                               gaagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCg                          >  1:3188674/1‑93 (MQ=255)

                                                               gaagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCg                          >  2:1631440/1‑93 (MQ=255)

                                                               gaagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCg                          >  2:2874653/1‑93 (MQ=255)

                                                                aagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGa                         <  1:2644443/93‑1 (MQ=255)

                                                                aagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGa                         <  2:2040115/93‑1 (MQ=255)

                                                                 agaTCGTTGTGCCACATAGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACGGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAt                        <  1:3781048/93‑1 (MQ=255)

                                                                  gaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAtt                       <  1:1240757/93‑1 (MQ=255)

                                                                  gaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAtt                       >  1:3507285/1‑93 (MQ=255)

                                                                  gaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAtt                       >  2:1501057/1‑93 (MQ=255)

                                                                    tCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGc                     >  2:39962/1‑93 (MQ=255)

                                                                      gTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGccc                   >  2:2231850/1‑93 (MQ=255)

                                                                      gTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGccc                   >  1:4551857/1‑93 (MQ=255)

                                                                      gTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGccc                   >  2:684208/1‑93 (MQ=255)

                                                                      gTTGTGCCACATGGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGccc                   <  1:4269472/93‑1 (MQ=255)

                                                                       ttGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGcccc                  <  1:3152158/93‑1 (MQ=255)

                                                                          tgCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGCTTGCCCCGGa               >  1:1895030/1‑93 (MQ=255)

                                                                          tgCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGa               >  1:3939485/1‑93 (MQ=255)

                                                                             cacaTTGGGGTTCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGaaaa            <  2:1029443/93‑1 (MQ=255)

                                                                             cacaTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATTTGCTGGAAAAACTGGCGATTGCCCCGGaaaa            <  1:3258299/93‑1 (MQ=255)

                                                                             cacaTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGaaaa            <  1:2413444/93‑1 (MQ=255)

                                                                              acaTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAt           <  2:1189072/93‑1 (MQ=255)

                                                                               caTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGTAAAAtg          <  2:3903583/93‑1 (MQ=255)

                                                                               caTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGCAAAtg          >  2:4105742/1‑93 (MQ=255)

                                                                               caTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtg          <  2:2469691/93‑1 (MQ=255)

                                                                                 ttGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgtg        <  1:3247475/93‑1 (MQ=255)

                                                                                 ttGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgtg        <  2:3874103/93‑1 (MQ=255)

                                                                                 ttGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgtg        >  1:2184608/1‑93 (MQ=255)

                                                                                   ggggATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAACTGAGGc      >  2:1924085/1‑93 (MQ=255)

                                                                                      gATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTa   <  2:378344/93‑1 (MQ=255)

CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340100‑3340279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`