BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,365,636	A→G	R59G (AGG→GGG)	yhcG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,365,636	0	A	G	99.6%	718.3 / ‑4.4	226	R59G (AGG→GGG)	yhcG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (81/144); minor base T (0/1); total (81/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

Only 100 of 236 total aligned reads displayed.

GAAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACG  >  NC_000913/3365546‑3365724

gaAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCggg                                                                                        >  1:3779110/1‑93 (MQ=255)

gaAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCggg                                                                                        >  2:660443/1‑93 (MQ=255)

  aaCGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGc                                                                                      <  2:2311101/93‑1 (MQ=255)

  aaCGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGc                                                                                      <  1:3930536/93‑1 (MQ=255)

     ggTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCCGGGCTgg                                                                                   >  2:3570704/1‑92 (MQ=255)

        aCGTAGCTTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTa                                                                                <  2:3702523/93‑1 (MQ=255)

        aCGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTa                                                                                <  2:4111289/93‑1 (MQ=255)

        aCGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTa                                                                                <  2:555360/93‑1 (MQ=255)

          gTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATg                                                                              >  2:2733086/1‑93 (MQ=255)

            aGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGt                                                                            <  2:839778/93‑1 (MQ=255)

             gCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTg                                                                           >  1:3711472/1‑93 (MQ=255)

             gCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTg                                                                           <  1:4684640/93‑1 (MQ=255)

               gTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTgcg                                                                         <  2:3466816/93‑1 (MQ=255)

                 tAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCa                                                                       <  1:1386956/93‑1 (MQ=255)

                   aCGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGc                                                                     <  2:1320059/93‑1 (MQ=255)

                   aCGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGc                                                                     <  2:431018/93‑1 (MQ=255)

                   aCGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGc                                                                     <  2:2843467/93‑1 (MQ=255)

                      cgTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTaa                                                                  <  2:3888888/93‑1 (MQ=255)

                      cgTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTaa                                                                  <  1:4288290/93‑1 (MQ=255)

                       gTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAAt                                                                 >  2:3598212/1‑93 (MQ=255)

                        ttAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATc                                                                >  1:3617788/1‑93 (MQ=255)

                        ttAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATc                                                                >  2:1012998/1‑93 (MQ=255)

                        ttAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATc                                                                <  1:754311/93‑1 (MQ=255)

                        ttAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATc                                                                <  2:4378271/93‑1 (MQ=255)

                          aaTGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCaa                                                              <  1:394875/93‑1 (MQ=255)

                          aaTGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCaa                                                              <  1:644777/93‑1 (MQ=255)

                           aTGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTAGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAg                                                             >  2:1250783/1‑93 (MQ=255)

                              aCCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGa                                                          >  1:2614509/1‑93 (MQ=255)

                               ccGCGACGTACCAGGAAATTTGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGAc                                                         <  1:2537822/93‑1 (MQ=255)

                               ccGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGAc                                                         <  1:627506/93‑1 (MQ=255)

                               ccGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGAc                                                         <  1:3510225/93‑1 (MQ=255)

                               ccGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGAc                                                         >  2:1384292/1‑93 (MQ=255)

                                cgcgACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACt                                                        >  2:3065248/1‑93 (MQ=255)

                                cgcgACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACt                                                        <  2:3898344/93‑1 (MQ=255)

                                 gcgACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTa                                                       <  1:605726/93‑1 (MQ=255)

                                 gcgACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTa                                                       <  1:2970574/93‑1 (MQ=255)

                                   gACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATc                                                     >  1:169224/1‑93 (MQ=255)

                                      gTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCaaa                                                  <  1:1631518/93‑1 (MQ=255)

                                      gTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCaaa                                                  >  1:2041596/1‑93 (MQ=255)

                                      gTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCaaa                                                  <  2:2974823/93‑1 (MQ=255)

                                      gTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCaaa                                                  <  2:3318940/93‑1 (MQ=255)

                                      gTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCaaa                                                  <  2:472203/93‑1 (MQ=255)

                                       tACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAg                                                 >  2:2648167/1‑93 (MQ=255)

                                        aCCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAgg                                                <  2:1987061/93‑1 (MQ=255)

                                        aCCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAgg                                                <  1:3243527/93‑1 (MQ=255)

                                         ccAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGa                                               >  2:1576998/1‑93 (MQ=255)

                                          cAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAt                                              <  1:4653813/93‑1 (MQ=255)

                                          cAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAt                                              <  1:105764/93‑1 (MQ=255)

                                           aGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAtt                                             <  1:3822479/93‑1 (MQ=255)

                                           aGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAtt                                             <  2:1790263/93‑1 (MQ=255)

                                           aGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAtt                                             <  2:458647/93‑1 (MQ=255)

                                           aGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAtt                                             >  2:2506439/1‑93 (MQ=255)

                                            ggAAAGTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGTGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGAttt                                            <  2:4023846/93‑1 (MQ=255)

                                             gAAATTGGCCGACTAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTa                                           <  1:703860/93‑1 (MQ=255)

                                             gAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTa                                           <  2:2067680/93‑1 (MQ=255)

                                             gAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTa                                           >  1:240645/1‑93 (MQ=255)

                                             gAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTa                                           <  2:4270588/93‑1 (MQ=255)

                                              aaaTTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTAt                                          <  1:2718865/93‑1 (MQ=255)

                                               aaTTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATg                                         <  2:633626/93‑1 (MQ=255)

                                               aaTTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATg                                         <  1:3586839/93‑1 (MQ=255)

                                                aTTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGt                                        >  2:3842702/1‑93 (MQ=255)

                                                aTTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGt                                        >  1:3333116/1‑93 (MQ=255)

                                                 ttGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCTGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTc                                       <  1:182865/93‑1 (MQ=255)

                                                 ttGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTc                                       <  1:2411574/93‑1 (MQ=255)

                                                   ggCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTa                                     >  1:1847016/1‑93 (MQ=255)

                                                   ggCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTa                                     >  1:1808261/1‑93 (MQ=255)

                                                     ccGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAg                                   <  2:1575136/93‑1 (MQ=255)

                                                     ccGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAg                                   <  2:3202833/93‑1 (MQ=255)

                                                       gacgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGt                                 >  2:1778010/1‑93 (MQ=255)

                                                        acgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGta                                <  2:2391991/93‑1 (MQ=255)

                                                        acgaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGta                                >  1:1207689/1‑93 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                              <  1:4112391/93‑1 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                              >  1:3033964/1‑93 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                              >  2:3105051/1‑93 (MQ=255)

                                                          gaATTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGtata                              >  1:1446976/1‑93 (MQ=255)

                                                           aaTTGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATaa                             <  2:696540/93‑1 (MQ=255)

                                                             ttGTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGc                           <  2:2988702/93‑1 (MQ=255)

                                                               gTCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGt                         >  2:2879786/1‑93 (MQ=255)

                                                                tCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTg                        <  1:40651/93‑1 (MQ=255)

                                                                tCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTg                        >  1:3302354/1‑93 (MQ=255)

                                                                tCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTg                        >  2:2224592/1‑93 (MQ=255)

                                                                tCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTg                        >  2:1082844/1‑93 (MQ=255)

                                                                tCGAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGAGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGGGTg                        >  1:3125009/1‑93 (MQ=255)

                                                                  gAATTTGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTggg                      >  1:902080/1‑93 (MQ=255)

                                                                     tttGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtc                   <  1:3536294/93‑1 (MQ=255)

                                                                      ttGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtct                  <  2:3931516/93‑1 (MQ=255)

                                                                       tGAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                 <  2:1878427/93‑1 (MQ=255)

                                                                        gAACAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTctct                <  1:3387789/93‑1 (MQ=255)

                                                                         aaCAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTg               >  1:1276220/1‑93 (MQ=255)

                                                                          aCAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGc              >  1:2909455/1‑93 (MQ=255)

                                                                          aCAAGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGc              >  1:1161079/1‑93 (MQ=255)

                                                                            aaGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCaa            >  1:3556594/1‑93 (MQ=255)

                                                                             aGGTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCaaa           >  1:2255314/1‑93 (MQ=255)

                                                                               gTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGcaacaa         >  1:2201575/1‑93 (MQ=255)

                                                                               gTGGCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCaaaaa         <  2:1188956/93‑1 (MQ=255)

                                                                                 ggCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAAc       >  1:4559703/1‑93 (MQ=255)

                                                                                  gCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACt      <  2:2554903/93‑1 (MQ=255)

                                                                                  gCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACt      <  1:2660322/93‑1 (MQ=255)

                                                                                  gCGAGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACt      >  2:74580/1‑93 (MQ=255)

                                                                                     aGGCCGGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTAc   <  1:1723546/93‑1 (MQ=255)

GAAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACG  >  NC_000913/3365546‑3365724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`