BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,054	A→G	H198R (CAC→CGC)	yhcG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,054	0	A	G	99.5%	654.6 / NA	211	H198R (CAC→CGC)	yhcG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (103/107); minor base C (1/0); total (104/107)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACACGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAG  >  NC_000913/3365964‑3366143

ggTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAAcgcg                                                                                         >  2:2430504/1‑93 (MQ=255)

    tGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGGCAAATCAGCCATGCTGCAACAACGCGCTcc                                                                                     >  2:479215/1‑93 (MQ=255)

    tGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTcc                                                                                     <  2:3935650/93‑1 (MQ=255)

    tGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTcc                                                                                     <  1:4223390/93‑1 (MQ=255)

      ttCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTg                                                                                   <  2:3518386/93‑1 (MQ=255)

       tCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGc                                                                                  <  1:196587/93‑1 (MQ=255)

        cGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGcc                                                                                 <  1:1478189/93‑1 (MQ=255)

           cAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgag                                                                              >  2:736376/1‑93 (MQ=255)

            aGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgaga                                                                             >  1:3784847/1‑93 (MQ=255)

            aGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgaga                                                                             <  1:4310030/93‑1 (MQ=255)

             gCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGAc                                                                            >  2:1869027/1‑93 (MQ=255)

              cTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACg                                                                           >  1:1074364/1‑93 (MQ=255)

                agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         <  2:2505947/93‑1 (MQ=255)

                agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         <  2:2934290/93‑1 (MQ=255)

                agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         <  1:1623395/93‑1 (MQ=255)

                agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         >  2:2951211/1‑93 (MQ=255)

                 gagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCat                                                                        <  2:4293892/93‑1 (MQ=255)

                   gCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCatat                                                                      >  2:1799407/1‑93 (MQ=255)

                    cGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAtt                                                                     <  1:4574053/93‑1 (MQ=255)

                      gCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGGCGCATAttct                                                                   >  2:2553828/1‑93 (MQ=255)

                        aaaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttcttc                                                                 <  2:3154505/93‑1 (MQ=255)

                        aaaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttcttc                                                                 >  1:3648720/1‑93 (MQ=255)

                         aaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTcc                                                                >  1:3683433/1‑93 (MQ=255)

                           ttGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGc                                                              >  2:4249419/1‑93 (MQ=255)

                             gCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCaa                                                            <  2:4025435/93‑1 (MQ=255)

                              cGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAAc                                                           <  1:2823865/93‑1 (MQ=255)

                               gACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          >  1:466461/1‑93 (MQ=255)

                               gACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          <  1:1829600/93‑1 (MQ=255)

                                aCCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAg                                                         <  1:4191146/93‑1 (MQ=255)

                                 cccAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAgg                                                        >  1:545176/1‑93 (MQ=255)

                                  ccAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGc                                                       >  1:4386806/1‑93 (MQ=255)

                                  ccAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCCCTCCTGCCGAGACGCATATTCTTCCGCAACAGGc                                                       >  1:712870/1‑93 (MQ=255)

                                    aGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCAATGCTGCAACAACCCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGcaa                                                     >  2:156559/1‑91 (MQ=255)

                                     gTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGAt                                                    <  2:2089747/93‑1 (MQ=255)

                                     gTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGAt                                                    <  2:1489913/93‑1 (MQ=255)

                                      ttttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATa                                                   <  1:2183555/93‑1 (MQ=255)

                                      ttttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATa                                                   <  1:435559/93‑1 (MQ=255)

                                      ttttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATa                                                   <  1:492520/93‑1 (MQ=255)

                                       tttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGTAACAGGCGATAc                                                  >  2:3186110/1‑93 (MQ=255)

                                       tttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATAc                                                  <  1:2807200/93‑1 (MQ=255)

                                          aTGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATAcgcg                                               >  2:3378936/1‑93 (MQ=255)

                                           tGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGa                                              >  2:2253341/1‑93 (MQ=255)

                                           tGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGa                                              >  2:4589997/1‑93 (MQ=255)

                                             aGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATc                                            >  2:1224147/1‑93 (MQ=255)

                                                 gACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCtt                                        <  2:2687944/93‑1 (MQ=255)

                                                  accctactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCttt                                       <  2:183000/90‑1 (MQ=255)

                                                   cacTCCTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtg                                      <  2:1022992/93‑1 (MQ=255)

                                                   cacTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtg                                      <  1:1250418/93‑1 (MQ=255)

                                                   cacTACTGTCACAAGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtg                                      >  2:4502338/1‑93 (MQ=255)

                                                    actactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtgt                                     <  2:569731/93‑1 (MQ=255)

                                                    actactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtgt                                     <  2:246261/93‑1 (MQ=255)

                                                     ctactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtgtg                                    <  1:3030310/93‑1 (MQ=255)

                                                       actGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCt                                  >  1:871580/1‑93 (MQ=255)

                                                        ctGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTCTGTGCTc                                 >  1:2282664/1‑93 (MQ=255)

                                                         tGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCg                                >  1:2884404/1‑93 (MQ=255)

                                                          gTCACATGACAAATCAGCCATGCTGCAACACCGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGa                               <  2:3469190/93‑1 (MQ=255)

                                                          gTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGa                               >  2:3293025/1‑93 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGACtt                            >  1:92900/1‑93 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            <  2:4676699/93‑1 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            <  2:3118578/93‑1 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            >  2:4175459/1‑93 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            <  2:4316888/93‑1 (MQ=255)

                                                              caTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAttt                           <  2:3876375/93‑1 (MQ=255)

                                                              caTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAttt                           >  1:2416394/1‑93 (MQ=255)

                                                              caTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAttt                           <  2:536082/93‑1 (MQ=255)

                                                               aTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTc                          >  1:2087052/1‑93 (MQ=255)

                                                               aTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTc                          <  1:2970862/93‑1 (MQ=255)

                                                                tcaCAAATCAGCCATGCTGCAACACCGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCt                         <  2:4272198/91‑1 (MQ=255)

                                                                tGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCt                         <  1:1325721/93‑1 (MQ=255)

                                                                 gACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTg                        >  1:1167875/1‑93 (MQ=255)

                                                                   cAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTgtc                      >  1:4503411/1‑91 (MQ=255)

                                                                   cAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGa                      <  1:3162403/93‑1 (MQ=255)

                                                                   cAAATCAGCCATGCTACAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGa                      <  1:2338460/93‑1 (MQ=255)

                                                                    aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGaa                     >  2:528632/1‑93 (MQ=255)

                                                                    aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGaa                     <  2:3058871/93‑1 (MQ=255)

                                                                    aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGaa                     >  2:1947053/1‑93 (MQ=255)

                                                                     aaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAt                    <  2:1303059/93‑1 (MQ=255)

                                                                     aaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAt                    <  1:3258568/93‑1 (MQ=255)

                                                                     aaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAt                    <  2:1174937/93‑1 (MQ=255)

                                                                      aTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAtt                   <  2:844590/93‑1 (MQ=255)

                                                                      aTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAtt                   >  2:372619/1‑93 (MQ=255)

                                                                      aTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAtt                   >  1:1757552/1‑93 (MQ=255)

                                                                       tCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTg                  <  1:770848/93‑1 (MQ=255)

                                                                       tCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTg                  >  1:2509808/1‑93 (MQ=255)

                                                                        cAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGa                 <  1:349436/93‑1 (MQ=255)

                                                                         aGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaa                <  2:4356441/93‑1 (MQ=255)

                                                                         aGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaa                <  1:257291/93‑1 (MQ=255)

                                                                          gCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaaa               <  2:4007572/93‑1 (MQ=255)

                                                                          gCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaaa               <  1:2671199/93‑1 (MQ=255)

                                                                            cATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGa             >  2:65124/1‑93 (MQ=255)

                                                                            cATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGa             <  1:3044683/93‑1 (MQ=255)

                                                                             aTGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGAt            <  2:4337417/93‑1 (MQ=255)

                                                                              tgctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATg           >  1:1123864/1‑93 (MQ=255)

                                                                              tgctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATg           <  1:1395540/93‑1 (MQ=255)

                                                                              tgctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATg           >  2:2137718/1‑93 (MQ=255)

                                                                               gctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGa          <  1:3347737/93‑1 (MQ=255)

                                                                                ctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGaa         <  1:2118688/93‑1 (MQ=255)

                                                                                ctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGaa         <  2:2386466/93‑1 (MQ=255)

                                                                                   caacaaCGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAatat      >  1:4000046/1‑93 (MQ=255)

                                                                                       aacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAg  >  1:814618/1‑93 (MQ=255)

GGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACACGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAG  >  NC_000913/3365964‑3366143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`