BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,746	G→A	A693T (GCC→ACC)	acrF →	AcrF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,746	0	G	A	99.5%	664.1 / NA	194	A693T (GCC→ACC)	acrF	AcrF
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (106/87); minor base C (0/1); total (106/88)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

TTCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGAC  >  NC_000913/3414657‑3414835

ttccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATAccc                                                                                        >  1:571906/1‑93 (MQ=255)

ttccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATAccc                                                                                        >  2:4348065/1‑93 (MQ=255)

ttccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATAccc                                                                                        >  2:4025020/1‑93 (MQ=255)

  ccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTa                                                                                      <  1:4261784/93‑1 (MQ=255)

   cattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTaa                                                                                     <  1:702608/93‑1 (MQ=255)

   cattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTaa                                                                                     >  1:2307868/1‑93 (MQ=255)

   cattcAATAGGCCAGCCATTGGTAAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTaa                                                                                     <  2:272646/93‑1 (MQ=255)

     ttcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTCAcc                                                                                   >  2:2556657/1‑93 (MQ=255)

     ttcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAcc                                                                                   <  2:1237028/93‑1 (MQ=255)

     ttcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAcc                                                                                   >  2:3248452/1‑93 (MQ=255)

      tcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAAccc                                                                                  >  1:318516/1‑93 (MQ=255)

        aaTATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAg                                                                                >  1:1683922/1‑93 (MQ=255)

        aaTATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAg                                                                                >  1:3541476/1‑93 (MQ=255)

        aaTATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAg                                                                                <  2:1361156/93‑1 (MQ=255)

         atatGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAgg                                                                               >  2:3420698/1‑93 (MQ=255)

         atatGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAgg                                                                               >  2:2837244/1‑93 (MQ=255)

          tatGCCAGCCATTGTTGAACTGGGCACGGCAACGGGGTTCGACTTTGAGTTAATTGATCAGGCTGCGCTGGGTCACGATACCCTAACCCAGGc                                                                              >  1:3480077/1‑93 (MQ=255)

             gccagccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCg                                                                           >  1:3007783/1‑93 (MQ=255)

                agccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTaa                                                                        >  1:1114117/1‑93 (MQ=255)

                agccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTaa                                                                        >  2:4057345/1‑93 (MQ=255)

                 gccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAAc                                                                       <  1:560253/93‑1 (MQ=255)

                 gccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAAc                                                                       >  2:2386293/1‑93 (MQ=255)

                 gccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAAc                                                                       >  1:2544721/1‑93 (MQ=255)

                  ccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAAcc                                                                      >  1:2381355/1‑93 (MQ=255)

                   caTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCa                                                                     >  2:3901309/1‑93 (MQ=255)

                   caTTGTTGAACTCGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCa                                                                     >  2:726376/1‑93 (MQ=255)

                    aTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAg                                                                    >  2:2774797/1‑93 (MQ=255)

                    aTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAg                                                                    >  2:2053220/1‑93 (MQ=255)

                      tgttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGtt                                                                  <  1:3828733/93‑1 (MQ=255)

                      tgttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTAACGATACCCTAACCCAGGCCCGTAACCAGtt                                                                  >  2:2231141/1‑93 (MQ=255)

                       gttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTg                                                                 <  2:2146141/93‑1 (MQ=255)

                         tgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGTGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCt                                                               <  1:2515886/93‑1 (MQ=255)

                          gagcTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCtt                                                              <  2:3164654/90‑1 (MQ=255)

                          gAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCtt                                                              >  1:4666348/1‑93 (MQ=255)

                          gAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGCTGCtt                                                              >  2:1849457/1‑93 (MQ=255)

                           aaCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTg                                                             <  2:1659724/93‑1 (MQ=255)

                           aaCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTg                                                             <  2:4369154/93‑1 (MQ=255)

                            aCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTgg                                                            >  1:1671571/1‑93 (MQ=255)

                            aCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTgg                                                            >  2:2283058/1‑93 (MQ=255)

                            aCTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTgg                                                            <  2:4233342/93‑1 (MQ=255)

                            aCTGGGCACCGCGACGGGTTTCGTCTTTGAGTTAATTGATTTGGCTGGGCTCGGGCACGACAGCGTAACCCAGCACCGTAACCAGATGCtctg                                                            >  2:2887652/1‑90 (MQ=255)

                             cTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGt                                                           <  1:4175788/93‑1 (MQ=255)

                              tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          <  2:3831500/93‑1 (MQ=255)

                              tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          >  2:842745/1‑93 (MQ=255)

                              tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTa                                                          >  1:4247375/1‑93 (MQ=255)

                               gggCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTAt                                                         <  1:4598228/93‑1 (MQ=255)

                                  cACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggc                                                      <  2:824068/93‑1 (MQ=255)

                                  cACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggc                                                      <  2:1905432/93‑1 (MQ=255)

                                   aCGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggcg                                                     >  1:893182/1‑93 (MQ=255)

                                   aCGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggcg                                                     >  2:782145/1‑93 (MQ=255)

                                    cGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggcgg                                                    >  2:1044892/1‑93 (MQ=255)

                                     ggCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATggcggc                                                   >  1:2933627/1‑93 (MQ=255)

                                      gCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGgcggcg                                                  >  1:4099099/1‑93 (MQ=255)

                                       cAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGgcgc                                                 <  2:1545677/93‑1 (MQ=255)

                                        aaCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCa                                                >  2:484297/1‑93 (MQ=255)

                                        aaCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGAGGAGCa                                                >  2:4212771/1‑93 (MQ=255)

                                         aCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCaa                                               <  1:3244595/93‑1 (MQ=255)

                                         aCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCaa                                               <  2:4143702/93‑1 (MQ=255)

                                            ggTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACAt                                            <  2:3138427/93‑1 (MQ=255)

                                             gTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATCCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATc                                           <  2:523746/93‑1 (MQ=255)

                                              tttCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATcc                                          >  1:1488719/1‑93 (MQ=255)

                                                  gACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGcc                                      <  1:2443146/93‑1 (MQ=255)

                                                  gACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAAAATCCTccc                                      >  2:175669/1‑93 (MQ=255)

                                                   aCTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACCTCCTGCCa                                     >  2:3967499/1‑93 (MQ=255)

                                                    cTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAg                                    >  2:3604091/1‑93 (MQ=255)

                                                    cTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAg                                    <  2:2517055/93‑1 (MQ=255)

                                                    cTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAg                                    >  1:3427639/1‑93 (MQ=255)

                                                     tttGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGc                                   >  2:2638168/1‑93 (MQ=255)

                                                     tttGAGTTAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGc                                   >  1:4550144/1‑93 (MQ=255)

                                                           ttAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTc                             <  1:733466/93‑1 (MQ=255)

                                                            tAATTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCa                            <  1:1371329/93‑1 (MQ=255)

                                                             aaTTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAg                           >  1:1890475/1‑93 (MQ=255)

                                                              aTTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGc                          <  1:257126/93‑1 (MQ=255)

                                                              aTTGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGc                          <  1:2656160/93‑1 (MQ=255)

                                                              aTTCATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGc                          <  1:2496800/93‑1 (MQ=255)

                                                               ttGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCg                         >  2:3480526/1‑93 (MQ=255)

                                                                tGATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGt                        <  1:2104528/93‑1 (MQ=255)

                                                                 gATCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTg                       >  1:1098505/1‑93 (MQ=255)

                                                                  aTCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTgg                      >  2:2961953/1‑92 (MQ=255)

                                                                  aTCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACAGCCTGCCAGCTTAGTCAGCGTgc                      <  1:1616963/93‑1 (MQ=255)

                                                                   tCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTgcg                     >  1:1211192/1‑93 (MQ=255)

                                                                   tCAGGCTGGGCTGGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTgcg                     >  2:583379/1‑93 (MQ=255)

                                                                      ggctgggctgGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGccc                  <  1:754607/93‑1 (MQ=255)

                                                                      ggctgggctgGGTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGccc                  <  1:3883531/93‑1 (MQ=255)

                                                                        ctgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTa                <  1:1836394/93‑1 (MQ=255)

                                                                        ctgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTa                <  1:935522/93‑1 (MQ=255)

                                                                         tgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTac               <  2:4502187/93‑2 (MQ=255)

                                                                         tgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTaa               <  1:3142938/93‑1 (MQ=255)

                                                                         tgggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTaa               <  2:4410823/93‑1 (MQ=255)

                                                                          gggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAAt              >  1:1033406/1‑93 (MQ=255)

                                                                          gggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAAt              <  1:4295375/93‑1 (MQ=255)

                                                                          gggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAAt              <  2:4485840/93‑1 (MQ=255)

                                                                          gggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAAt              <  1:3312425/93‑1 (MQ=255)

                                                                           ggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATg             >  1:3664497/1‑93 (MQ=255)

                                                                           ggctgggTCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATg             >  2:335784/1‑93 (MQ=255)

                                                                            gctgggTCACAATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATgg            >  1:1659560/1‑93 (MQ=255)

                                                                                  tCACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATAGCCTGGa      >  1:815425/1‑93 (MQ=255)

                                                                                   cACGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGaa     <  2:1933909/93‑1 (MQ=255)

                                                                                     cGATCCCATAACCCAGCCCCGTAACCAGTTGCTTGGTATGGCGGCGGAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGa   <  2:3801695/93‑1 (MQ=255)

                                                                                     cGATACCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGa   <  2:2531954/93‑1 (MQ=255)

TTCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGAC  >  NC_000913/3414657‑3414835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`