BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,427,261	T→C	V130A (GTC→GCC)	b3279 →	putative transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427,261	0	T	C	99.5%	600.6 / NA	190	V130A (GTC→GCC)	b3279	putative transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (92/96); minor base G (1/0); total (93/97)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

 tGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGcccc                                                                                        >  1:3902896/1‑93 (MQ=255)

  ggCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCca                                                                                       >  2:2734989/1‑93 (MQ=255)

   gCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCcac                                                                                      <  1:353849/93‑1 (MQ=255)

     aaTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaa                                                                                    >  1:721297/1‑93 (MQ=255)

      aTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaa                                                                                   >  2:1002687/1‑93 (MQ=255)

       tCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGTGTAGTCTGGCCCCACaaaa                                                                                  >  1:1657754/1‑93 (MQ=255)

       tCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaaa                                                                                  <  1:1728822/93‑1 (MQ=255)

        cGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 <  2:3324450/93‑1 (MQ=255)

        cGAGTTGTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 <  1:1022608/93‑1 (MQ=255)

          aGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaa                                                                               <  1:3679126/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  2:2629556/93‑1 (MQ=255)

           gTTGTGGTGGGGATGTGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGACCCACAAAATaaa                                                                              <  2:3549466/93‑1 (MQ=255)

            ttttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAc                                                                             >  1:1520463/1‑93 (MQ=255)

            ttttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAc                                                                             >  2:2280332/1‑93 (MQ=255)

             tttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACg                                                                            <  1:4103176/93‑1 (MQ=255)

              ttggttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACgg                                                                           >  2:4483977/1‑93 (MQ=255)

                ggttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCt                                                                         <  2:315032/93‑1 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  2:1887375/93‑1 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  1:2826981/93‑1 (MQ=255)

                    gggATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGgag                                                                     >  2:2804636/1‑93 (MQ=255)

                       aTGGTCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGc                                                                  <  2:3094192/93‑1 (MQ=255)

                       aTGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGc                                                                  <  2:2080936/93‑1 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCg                                                                 <  2:3183246/93‑1 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCg                                                                 >  1:3422230/1‑93 (MQ=255)

                          ggCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTTGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGa                                                               <  2:3819506/93‑1 (MQ=255)

                          ggCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGa                                                               <  1:3879779/93‑1 (MQ=255)

                           gCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGat                                                              <  1:188316/93‑1 (MQ=255)

                            cTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGata                                                             <  2:3280908/93‑1 (MQ=255)

                             tCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGatat                                                            >  2:798982/1‑93 (MQ=255)

                              cAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATc                                                           <  1:2902430/93‑1 (MQ=255)

                              cAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATc                                                           <  2:632966/93‑1 (MQ=255)

                               aaTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCt                                                          >  1:4122917/1‑93 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         >  1:705518/1‑93 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         <  1:4572380/93‑1 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         <  1:2256015/93‑1 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         >  2:4122447/1‑93 (MQ=255)

                                aTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTg                                                         <  1:1286554/93‑1 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        >  1:592825/1‑93 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        >  1:4521651/1‑93 (MQ=255)

                                     aCTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtct                                                    >  1:1197645/1‑93 (MQ=255)

                                       ttGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCg                                                  >  1:3270552/1‑93 (MQ=255)

                                         gATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGt                                                <  1:4006732/93‑1 (MQ=255)

                                          aTGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTa                                               <  1:3609143/93‑1 (MQ=255)

                                           tGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAg                                              >  2:1768813/1‑93 (MQ=255)

                                              cgcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGccc                                           <  2:4187024/93‑1 (MQ=255)

                                              cgcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGccc                                           <  1:1125475/93‑1 (MQ=255)

                                               gcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGcccc                                          >  2:3088279/1‑93 (MQ=255)

                                                cAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGCGAGCGGATATCTGTATCTCGGTAGCCCCg                                         >  2:1470444/1‑93 (MQ=255)

                                                cAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCg                                         >  1:1318323/1‑93 (MQ=255)

                                                cAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCg                                         <  2:3903127/93‑1 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGt                                        <  2:293290/93‑1 (MQ=255)

                                                 aaTAGTAGAAGATGATGTGATGATTGGCGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGt                                        >  2:2142316/1‑93 (MQ=255)

                                                  aTAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTc                                       >  1:1074108/1‑93 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      <  2:1747663/93‑1 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      >  2:1602388/1‑93 (MQ=255)

                                                   tagtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCa                                      >  2:1259808/1‑93 (MQ=255)

                                                    agtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCaa                                     >  1:1960099/1‑93 (MQ=255)

                                                     gtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCaaa                                    >  2:4652577/1‑93 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAAc                                   <  1:530311/93‑1 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  2:1379554/1‑93 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  1:268232/1‑93 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  2:1649147/1‑93 (MQ=255)

                                                        gaagaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAg                                 >  1:3656333/1‑93 (MQ=255)

                                                          agaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGAt                               >  2:681931/1‑93 (MQ=255)

                                                          agaTGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGAt                               <  1:3023222/93‑1 (MQ=255)

                                                           gatgatGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATc                              <  2:924331/93‑1 (MQ=255)

                                                            atgatgTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATcc                             >  1:3734146/1‑93 (MQ=255)

                                                             tgatgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCg                            >  1:1573992/1‑93 (MQ=255)

                                                              gatgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGc                           >  1:3224258/1‑93 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTCTCTCGGTAGCCCCGTCAAACAGATCCGcc                          >  1:1399005/1‑93 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGcc                          <  2:1087688/93‑1 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGcc                          >  1:4654180/1‑93 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCAGGCCCCACAAAATAAACGTCTGGAGAGCGGATATCTTTATCTCGGTAGCCCCGTCAAACAGGTCCGcc                          <  2:538652/93‑1 (MQ=255)

                                                                   gatgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGtt                      >  1:3277116/1‑93 (MQ=255)

                                                                       atTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGt                  >  1:432633/1‑93 (MQ=255)

                                                                       atTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGt                  <  1:3314207/93‑1 (MQ=255)

                                                                        tgggTGCGGTTAGTCGGCCCCCAAAAAATAACCGGCGGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtg                 <  2:3737179/91‑1 (MQ=255)

                                                                         tGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgt                >  1:2332364/1‑92 (MQ=255)

                                                                         tGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtga                <  1:755929/93‑1 (MQ=255)

                                                                          tgTGCGGATATTCTGCCCCCAAAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgat               <  2:2480310/92‑1 (MQ=255)

                                                                          ggTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgat               <  1:3243968/93‑1 (MQ=255)

                                                                           gTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgatg              <  1:4136809/93‑1 (MQ=255)

                                                                           gTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtgatg              <  1:291692/93‑1 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  1:4460350/93‑1 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  2:2648795/93‑1 (MQ=255)

                                                                              cGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAag           >  1:1343875/1‑93 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          >  1:1787521/1‑93 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          >  2:2209806/1‑93 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          <  2:186080/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         <  1:647765/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         <  1:2576068/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         >  1:355791/1‑93 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        <  2:2364432/93‑1 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        <  1:1461416/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       <  1:3393970/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       <  1:1908402/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       >  2:4550735/1‑93 (MQ=255)

                                                                                    gTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAgg     >  2:1048746/1‑93 (MQ=255)

                                                                                      cTGGCCCCACAAAATAACCGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGACAAGGCt   <  2:2018030/93‑1 (MQ=255)

                                                                                       tGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTg  <  1:1144549/93‑1 (MQ=255)

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`