BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,971	T→C	G259G (GGT→GGC)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,971	0	T	C	99.1%	693.8 / ‑3.7	225	G259G (GGT→GGC)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (105/118); total (107/118)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 240 total aligned reads displayed.

GGTGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGTGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACGA  >  NC_000913/3544881‑3545060

ggTGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGa                                                                                         >  1:947242/1‑93 (MQ=255)

 gTGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGAc                                                                                        <  1:208764/93‑1 (MQ=255)

   gCCGGTATTACTGATGGCGATGCGTGCGATTGCCTAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGAccc                                                                                      <  2:3054488/93‑1 (MQ=255)

     cGGTAGTATTGATGGCGATGCGTGCGATTGCCAAATTGATCCTGCCAAAAGTTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCgg                                                                                    <  2:3979413/93‑1 (MQ=255)

      ggTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCgga                                                                                   >  1:3253482/1‑92 (MQ=255)

        tagtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTaa                                                                                 >  1:597502/1‑93 (MQ=255)

         agtaCTGATGGCGATGCGTGCGATTGCCGAAATGAGCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAAt                                                                                <  2:3363429/93‑1 (MQ=255)

          gtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATg                                                                               <  1:419725/93‑1 (MQ=255)

          gtaCTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATg                                                                               <  1:2352353/93‑1 (MQ=255)

              tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           <  1:3382564/93‑1 (MQ=255)

              tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           <  1:1584414/93‑1 (MQ=255)

              tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCaa                                                                           >  2:3658582/1‑93 (MQ=255)

              tGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGAGGAGATCCTCGTCCCCCCGGTAATGCCaa                                                                           >  2:2216203/1‑93 (MQ=255)

               gATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGGCACGCTTTCCTGCCGGTAGCGGCGTTCCTCGGCGCACCGGTAATGGCAAc                                                                          >  1:1623311/1‑93 (MQ=255)

                 tGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGc                                                                        <  1:4599198/93‑1 (MQ=255)

                 gggCGATGCTTGCGATTCCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGc                                                                        <  2:755328/92‑1 (MQ=255)

                   gCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTg                                                                      >  1:2168646/1‑93 (MQ=255)

                    cGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGa                                                                     <  2:455279/93‑1 (MQ=255)

                     gaggcgtgcgATTGCAGAAATGATCCTGCAGAAAGCTCACGCTTCCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAt                                                                    <  1:363794/90‑1 (MQ=255)

                     gATGCGTGCGTTTCCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAt                                                                    <  2:649915/93‑1 (MQ=255)

                     gATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGTGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAt                                                                    <  1:4670096/93‑1 (MQ=255)

                     gATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAt                                                                    >  1:2471356/1‑93 (MQ=255)

                     gATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAt                                                                    <  2:330272/93‑1 (MQ=255)

                      aTGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTCATGGCAACGCTGAtt                                                                   >  2:3558005/1‑93 (MQ=255)

                      aTGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAtt                                                                   <  2:160488/93‑1 (MQ=255)

                      aTGCGTGCGATTGCCAAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGAtt                                                                   <  1:4050795/93‑1 (MQ=255)

                        gcgtgcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGc                                                                 <  1:2120319/93‑1 (MQ=255)

                          gtgcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGCGTTCCTCGGCGACCCGGTAATGGCCACGATGATTGCCg                                                               >  2:1736813/1‑93 (MQ=255)

                           tgcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGt                                                              <  2:2650265/93‑1 (MQ=255)

                           tgcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGt                                                              <  1:4541830/93‑1 (MQ=255)

                            gcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTg                                                             >  1:3797024/1‑93 (MQ=255)

                            gcgATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTg                                                             <  1:1683048/93‑1 (MQ=255)

                              gATTGACGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCt                                                           <  1:2386270/93‑1 (MQ=255)

                                  gCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGAtt                                                       <  2:2387240/93‑1 (MQ=255)

                                    cGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGc                                                     <  1:204247/93‑1 (MQ=255)

                                     gAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCg                                                    <  1:2626540/93‑1 (MQ=255)

                                      aaaTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGa                                                   >  2:2089464/1‑93 (MQ=255)

                                       aaggATCCTGCGAAAAGGTCACGTTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAt                                                  <  2:4244401/90‑1 (MQ=255)

                                       aaTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCTGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAt                                                  <  2:3163563/93‑1 (MQ=255)

                                       aaTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAt                                                  <  2:852278/93‑1 (MQ=255)

                                       aaTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAt                                                  >  1:3272472/1‑93 (MQ=255)

                                        aTGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATg                                                 >  1:81866/1‑93 (MQ=255)

                                         tGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGt                                                >  1:3955820/1‑93 (MQ=255)

                                          gATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGtt                                               >  2:1198220/1‑93 (MQ=255)

                                          gATCCTGCCGAAAGGTCACGCTTTCCTGCCGGAAGCGGAGTTCCTCGGGGCCCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGAtttt                                               >  2:4410014/1‑93 (MQ=255)

                                           aTCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTc                                              >  1:2128784/1‑93 (MQ=255)

                                           aTCCGGCCGAAAGGTCACGCTTCCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTc                                              <  2:1816437/93‑1 (MQ=255)

                                              cTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCAcc                                           <  2:3698359/93‑1 (MQ=255)

                                               tGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCt                                          >  1:4325115/1‑93 (MQ=255)

                                                gCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCGTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCtt                                         <  2:696763/93‑1 (MQ=255)

                                                 ccGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCttt                                        <  1:580312/93‑1 (MQ=255)

                                                  cGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTg                                       >  1:1141271/1‑93 (MQ=255)

                                                   gAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTgg                                      >  1:4445652/1‑93 (MQ=255)

                                                     aaGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTc                                    >  1:1888744/1‑93 (MQ=255)

                                                     aaGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTc                                    >  1:3475680/1‑93 (MQ=255)

                                                     aaGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTc                                    >  1:3547411/1‑93 (MQ=255)

                                                      agctCACGCTTCCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCt                                   <  1:959600/90‑1 (MQ=255)

                                                       ggTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTa                                  <  2:172163/93‑1 (MQ=255)

                                                         tCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTaaa                                <  1:1010373/93‑1 (MQ=255)

                                                          cACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAAc                               <  2:392955/93‑1 (MQ=255)

                                                           aCGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAAcc                              >  1:716236/1‑93 (MQ=255)

                                                            cGCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCg                             <  2:612388/93‑1 (MQ=255)

                                                             gCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGt                            >  2:2277262/1‑93 (MQ=255)

                                                             gCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGt                            >  2:2851484/1‑93 (MQ=255)

                                                             gCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGt                            <  2:4328363/93‑1 (MQ=255)

                                                             gCTTTCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGt                            >  1:4628173/1‑93 (MQ=255)

                                                               tttCCTGCCGGTAGCGGAGTTCCTCTGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTgg                          >  2:2126170/1‑93 (MQ=255)

                                                               tttCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTgg                          <  2:4081718/93‑1 (MQ=255)

                                                               tttCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTgg                          >  1:1316669/1‑93 (MQ=255)

                                                               tttCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTgg                          >  1:3715096/1‑93 (MQ=255)

                                                                ttCCTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGt                         <  2:1084095/93‑1 (MQ=255)

                                                                tcccTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGt                         <  1:292028/91‑1 (MQ=255)

                                                                tcccTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGt                         <  2:1397580/91‑1 (MQ=255)

                                                                   cTGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGt                      <  1:2266309/93‑1 (MQ=255)

                                                                    tGCCGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGtt                     >  2:515240/1‑93 (MQ=255)

                                                                      ccGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCa                   >  1:2843863/1‑93 (MQ=255)

                                                                       cGGTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCaa                  >  1:841307/1‑93 (MQ=255)

                                                                        ggTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAAt                 <  2:2748734/93‑1 (MQ=255)

                                                                         gTAGCGGAGTTCCTCGGCGACCCGTTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATg                <  1:2796992/93‑1 (MQ=255)

                                                                         gTAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATg                <  1:4138015/93‑1 (MQ=255)

                                                                          tAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATgg               <  2:4129775/93‑1 (MQ=255)

                                                                          tAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATgg               <  2:3435877/93‑1 (MQ=255)

                                                                          tAGCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATgg               <  2:1965020/93‑1 (MQ=255)

                                                                           accGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCTTGCTGATTGCGTTGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGa              <  2:2529836/91‑1 (MQ=255)

                                                                            gCGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGAt             <  1:1233185/93‑1 (MQ=255)

                                                                             cGGAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATc            >  2:1266718/1‑93 (MQ=255)

                                                                              ggAGTTCCTCGGTGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGCTCAATGGATCa           >  2:1574781/1‑93 (MQ=255)

                                                                               gAGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAg          <  2:3466088/93‑1 (MQ=255)

                                                                                aGTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGa         <  2:879624/93‑1 (MQ=255)

                                                                                 gTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGAt        >  1:2450456/1‑93 (MQ=255)

                                                                                 gTTCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGAt        <  1:196422/93‑1 (MQ=255)

                                                                                   tCCTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGCTTa      >  1:52712/1‑93 (MQ=255)

                                                                                    gcTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTaa     >  1:4424293/2‑93 (MQ=255)

                                                                                    ccTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTaa     <  1:3058983/93‑1 (MQ=255)

                                                                                    ccTCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTaa     <  2:746633/93‑1 (MQ=255)

                                                                                     cTCGGCGCCCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAAc    <  2:2354006/93‑1 (MQ=255)

                                                                                      tCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACg   >  1:1512638/1‑93 (MQ=255)

                                                                                      tCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACg   >  2:785499/1‑93 (MQ=255)

                                                                                      tCGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGAATAACg   <  2:2625855/93‑1 (MQ=255)

                                                                                       cGGCGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACGa  <  2:2997089/93‑1 (MQ=255)

GGTGCCGGTAGTACTGATGGCGATGCGTGCGATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGTGACCCGGTAATGGCAACGCTGATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACGA  >  NC_000913/3544881‑3545060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`