BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,668	G→A	L93L (TTG→TTA)	yrhB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,668	0	G	A	97.5%	551.4 / NA	162	L93L (TTG→TTA)	yrhB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (127/31); minor base C (0/4); total (127/35)
Fisher's exact test for biased strand distribution p-value = 1.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATG  >  NC_000913/3582578‑3582755

aGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAcc                                                                                       >  2:2420880/1‑93 (MQ=255)

aGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAcc                                                                                       >  1:1147085/1‑93 (MQ=255)

 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAccc                                                                                      >  2:3007630/1‑93 (MQ=255)

 gACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTAccc                                                                                      >  2:2839968/1‑93 (MQ=255)

  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTATCTTCGGCTTACCCt                                                                                     >  2:3960764/1‑93 (MQ=255)

  aCAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCt                                                                                     >  1:2166628/1‑93 (MQ=255)

   cAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTg                                                                                    >  2:2894263/1‑93 (MQ=255)

    aGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGa                                                                                   <  1:932069/93‑1 (MQ=255)

     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAc                                                                                  >  2:2229620/1‑93 (MQ=255)

     gtggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGAc                                                                                  <  1:3152351/93‑1 (MQ=255)

      tggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACa                                                                                 >  1:2438028/1‑93 (MQ=255)

      tggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACa                                                                                 >  2:3567310/1‑93 (MQ=255)

       ggtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACaa                                                                                <  1:4493386/93‑1 (MQ=255)

        gtgAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACaaa                                                                               <  2:655268/93‑1 (MQ=255)

          gAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAAt                                                                             >  1:2274295/1‑93 (MQ=255)

           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATa                                                                            >  2:2054993/1‑93 (MQ=255)

           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATa                                                                            <  2:754548/93‑1 (MQ=255)

           aaaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATa                                                                            >  1:3500175/1‑93 (MQ=255)

            aaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATac                                                                           >  1:486719/1‑92 (MQ=255)

            aaTTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAg                                                                           <  1:1595363/93‑1 (MQ=255)

             attcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGc                                                                          >  1:1615259/1‑93 (MQ=255)

              ttcattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGcc                                                                         >  1:576442/1‑93 (MQ=255)

                cattcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCct                                                                       <  2:4356118/93‑1 (MQ=255)

                  ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCctct                                                                     >  1:795842/1‑93 (MQ=255)

                  ttcTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCctct                                                                     >  1:1162468/1‑93 (MQ=255)

                   tctcTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCtt                                                                    <  1:3906337/93‑1 (MQ=255)

                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                  >  2:334092/1‑93 (MQ=255)

                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                  >  1:2151285/1‑93 (MQ=255)

                     tctGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTcc                                                                  >  2:2515118/1‑93 (MQ=255)

                          gAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGa                                                             <  1:513816/93‑1 (MQ=255)

                          gAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGa                                                             >  1:4542699/1‑93 (MQ=255)

                            aCGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAag                                                           >  2:477288/1‑93 (MQ=255)

                             cGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAaga                                                          <  1:3935345/93‑1 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         <  1:2344348/93‑1 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         >  1:3324246/1‑93 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         <  2:4386686/93‑1 (MQ=255)

                              ggCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         >  2:3423967/1‑93 (MQ=255)

                              ggCAAAACCGCTGAAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAagag                                                         <  2:1228430/93‑1 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                      <  2:264604/93‑1 (MQ=255)

                                 aaaaCCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGc                                                      <  2:741915/93‑1 (MQ=255)

                                         tGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAAcc                                              >  1:1849867/1‑93 (MQ=255)

                                          ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCt                                             >  2:4112234/1‑93 (MQ=255)

                                          ggAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCt                                             >  2:101876/1‑93 (MQ=255)

                                            aagaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTa                                           <  1:3005379/93‑1 (MQ=255)

                                             agaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                          >  2:4655890/1‑93 (MQ=255)

                                             agaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAg                                          <  1:3563341/93‑1 (MQ=255)

                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAga                                         >  2:1047404/1‑92 (MQ=255)

                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGt                                         >  2:4436489/1‑93 (MQ=255)

                                              gaaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGt                                         >  1:354396/1‑93 (MQ=255)

                                               aaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTa                                        >  2:3865589/1‑93 (MQ=255)

                                               aaTATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGACCTCTTCCCACGAAGAGGGCCGCTAACCTTCGTa                                        >  2:2339799/1‑93 (MQ=255)

                                                atatCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAc                                       <  1:3023991/93‑1 (MQ=255)

                                                 tatCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAcc                                      >  1:1408850/1‑93 (MQ=255)

                                                     tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgc                                  >  1:1632367/1‑93 (MQ=255)

                                                     tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACACTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgc                                  >  2:2735683/1‑93 (MQ=255)

                                                       cAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCccgccg                                >  1:4416118/1‑93 (MQ=255)

                                                        aGGATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGt                               >  2:1980991/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                              >  1:901/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                              >  2:1117541/1‑93 (MQ=255)

                                                         ggATTACGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGtt                              >  2:2027096/1‑93 (MQ=255)

                                                            ttACGAAATAAAAAACGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGCGCCGCTAACCTTAGTACCCCGCCGtttat                           >  2:914282/1‑90 (MQ=255)

                                                             tACGAAAAAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAat                          <  1:4293997/93‑1 (MQ=255)

                                                              acaaaaaaaaaaaGGCTCCCTTCGGTTTCCCCTGACAAAATACCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatc                         <  2:3479802/90‑1 (MQ=255)

                                                              aCGAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatc                         >  1:4237329/1‑93 (MQ=255)

                                                               caaaaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatca                        <  2:2149623/91‑1 (MQ=255)

                                                               aaaaaaaaaaaaGCCTCCCTTCGCTTTCCCTTAAAAAAATACCCCTCTCCCCACAAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatca                        <  2:3187203/91‑1 (MQ=255)

                                                                gAAATAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcat                       >  1:2666509/1‑93 (MQ=255)

                                                                aaaaTAAAAAAGTCTCCCTTGGGCTTACCCGGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTACCCTTAGTCCCCCGCCGTTAAatcat                       <  2:1939811/92‑1 (MQ=255)

                                                                 aaaTAAAAAAGGCTCCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAatcatc                      <  2:745606/93‑1 (MQ=255)

                                                                 aaaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAatcatc                      >  1:806981/1‑93 (MQ=255)

                                                                 aaaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCAGCTAACCTTAGTACCCCGCCGTTAAatcatg                      >  2:2520346/1‑92 (MQ=255)

                                                                  aaTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTCCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATcc                     <  1:2904338/93‑1 (MQ=255)

                                                                   aTAAAAAAGGCTCCCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCa                    <  1:1461515/93‑1 (MQ=255)

                                                                   aTAAAAAAGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCa                    >  2:3580508/1‑93 (MQ=255)

                                                                    aaaaaaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCAc                   <  2:1482145/92‑1 (MQ=255)

                                                                     aaaaaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCTTCTTCCCATGAAAAGGGCCCCTAACCTTATTACCCCGCCGTTAAATCATCCAcc                  >  2:1442543/1‑93 (MQ=255)

                                                                     aaaaaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCAcc                  >  1:3179428/1‑93 (MQ=255)

                                                                       aaaaGGCTACCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGCGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGa                <  1:3302944/93‑1 (MQ=255)

                                                                        aaaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAg               >  2:2588005/1‑93 (MQ=255)

                                                                         aaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgc              >  1:373440/1‑93 (MQ=255)

                                                                         aaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgc              >  2:3693106/1‑93 (MQ=255)

                                                                         aaGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgc              >  1:4006353/1‑93 (MQ=255)

                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  2:1755749/1‑93 (MQ=255)

                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  2:839501/1‑93 (MQ=255)

                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  1:4599918/1‑93 (MQ=255)

                                                                          aGGCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg             >  2:4608702/1‑93 (MQ=255)

                                                                           ggCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAgcgc            <  1:3850364/93‑1 (MQ=255)

                                                                           ggCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcgc            >  2:4057750/1‑93 (MQ=255)

                                                                           ggCTACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcgc            >  1:3408565/1‑93 (MQ=255)

                                                                             cgaCCTTCGGCTTCCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTACCCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCgg          <  2:1364068/91‑1 (MQ=255)

                                                                              tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg         >  1:2582060/1‑93 (MQ=255)

                                                                              tACCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCggg         >  1:3100698/1‑93 (MQ=255)

                                                                               cccTTGGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGt        <  2:1327908/92‑1 (MQ=255)

                                                                               aCCTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAGTCATCCACCGAGCGCGGGt        >  1:2655951/1‑93 (MQ=255)

                                                                                 cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg      >  2:4182771/1‑93 (MQ=255)

                                                                                 cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg      >  1:2251047/1‑93 (MQ=255)

                                                                                 cTTCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCg      >  1:2009387/1‑93 (MQ=255)

                                                                                  ttCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg     >  1:44907/1‑93 (MQ=255)

                                                                                  ttCGGCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg     >  2:1486556/1‑93 (MQ=255)

                                                                                   tCGCCTTACCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa    <  2:2382018/93‑1 (MQ=255)

AGACAGTGGTGAAATTCATTCTCTGGGAACGGCAAAACCGCTGGAAGAATATCTACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATG  >  NC_000913/3582578‑3582755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`