BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,754,310	C→A	R382M (AGG→ATG)	yiaY ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,754,310	0	C	A	100.0%	934.1 / NA	255	R382M (AGG→ATG)	yiaY	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (127/128); total (127/128)

Only 100 of 257 total aligned reads displayed.

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGC‑‑‑ATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCC  >  NC_000913/3754220‑3754396

gACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATc                                                                                         <  1:1016240/93‑1 (MQ=255)

 aCAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCg                                                                                        >  2:3647178/1‑93 (MQ=255)

  cAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                       <  2:4162271/93‑1 (MQ=255)

  cAGCTATAAAGAATATACGGGAAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGc                                                                                       <  2:909954/93‑1 (MQ=255)

   aGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCt                                                                                      <  2:2468377/93‑1 (MQ=255)

    gCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTg                                                                                     >  1:4205998/1‑93 (MQ=255)

     cTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                    >  1:14107/1‑93 (MQ=255)

     cTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                    <  2:1149152/93‑1 (MQ=255)

     cTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGc                                                                                    <  1:2406276/93‑1 (MQ=255)

      tATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCg                                                                                   >  1:4272810‑M1/1‑92 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  >  2:2371381‑M1/1‑91 (MQ=255)

       aTCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgc                                                                                  <  2:4195936‑M1/93‑3 (MQ=255)

        tCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcg                                                                                 >  2:2282698‑M1/1‑90 (MQ=255)

         cACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcga                                                                                <  1:3528496‑M1/93‑5 (MQ=255)

          aCGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgat                                                                               <  1:1998998‑M1/93‑6 (MQ=255)

          aCGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgat                                                                               <  1:4540626‑M1/93‑6 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                            <  2:3130456‑M1/93‑9 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                            <  2:1978418‑M1/93‑9 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                            <  1:3017308‑M1/93‑9 (MQ=255)

             aaTTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaa                                                                            <  2:3715730‑M1/93‑9 (MQ=255)

               tttACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatc                                                                          >  1:931427‑M1/1‑83 (MQ=255)

                  aCGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgcc                                                                       <  2:1501217‑M1/93‑14 (MQ=255)

                  aCGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgcc                                                                       >  2:54542‑M1/1‑80 (MQ=255)

                   cGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgcca                                                                      <  2:2746080‑M1/93‑15 (MQ=255)

                      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacat                                                                   <  1:4424932‑M1/93‑18 (MQ=255)

                      gCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaa                                                                   <  1:4044053‑M1/93‑18 (MQ=255)

                       cAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaat                                                                  <  2:471061‑M1/93‑19 (MQ=255)

                       cAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaat                                                                  <  2:1527862‑M1/93‑19 (MQ=255)

                       cAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaat                                                                  <  2:4075206‑M1/93‑19 (MQ=255)

                        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                                                 <  1:4659651‑M1/93‑20 (MQ=255)

                        aaGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatt                                                                 <  1:4610180‑M1/93‑20 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                               >  1:1231353‑M1/1‑72 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                               <  2:2946625‑M1/93‑22 (MQ=255)

                          gggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttc                                                               <  2:3799325‑M1/93‑22 (MQ=255)

                           ggctacaGAAAAACTTGAAAAACCGGCAAACAATTTGCCACCCGGTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                                              <  2:1099516‑M1/90‑23 (MQ=255)

                           ggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                                              >  2:457631‑M1/1‑71 (MQ=255)

                           ggTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttct                                                              <  1:4535325‑M1/93‑23 (MQ=255)

                             ttACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttc                                                            >  1:2440969‑M1/1‑69 (MQ=255)

                             ttACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttc                                                            >  2:1259011‑M1/1‑69 (MQ=255)

                              tACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcg                                                           <  1:2659820‑M1/93‑26 (MQ=255)

                               aCAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgt                                                          <  2:3550761‑M1/93‑27 (MQ=255)

                                 agagATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtga                                                        <  1:2727478‑M1/93‑29 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                       >  1:3982589‑M1/1‑64 (MQ=255)

                                  gagaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgag                                                       >  1:3692007‑M1/1‑64 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     <  1:1527672‑M1/93‑32 (MQ=255)

                                    gaTAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagtt                                                     >  2:1183202‑M1/1‑62 (MQ=255)

                                      tAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGGTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgc                                                   >  2:428011‑M1/1‑60 (MQ=255)

                                       aGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcc                                                  <  2:4605349‑M1/93‑35 (MQ=255)

                                         ttGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctg                                                >  1:3066258‑M1/1‑57 (MQ=255)

                                            aaaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggat                                             <  1:786999‑M1/93‑40 (MQ=255)

                                             aaaaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatc                                            >  1:4406262‑M1/1‑53 (MQ=255)

                                               aaGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgg                                          <  1:994268‑M1/93‑43 (MQ=255)

                                                 gCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggt                                        >  2:1856704‑M1/1‑49 (MQ=255)

                                                  cGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggtt                                       <  1:1358800‑M1/93‑46 (MQ=255)

                                                    gCTAACAATTTGCCAGCCTTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                     <  1:3442166‑M1/93‑48 (MQ=255)

                                                    gCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttag                                     <  2:2891285‑M1/93‑48 (MQ=255)

                                                     cTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagt                                    <  2:3325463‑M1/93‑49 (MQ=255)

                                                       aaCAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaa                                  >  1:3904724‑M1/1‑43 (MQ=255)

                                                          aaTTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagc                               <  1:1690906‑M1/93‑54 (MQ=255)

                                                          aaTTTGCCAGCCGGTATGGAAATAATGATTACATCGCTGCgcgataaatcgccacaatttcgtcgtgagtggcctggatcggggtagtaaagc                               >  2:2328571‑M1/1‑40 (MQ=255)

                                                          aaTTCGCCAGCCGTTGTGGAAATGATGATTACATCGGTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagc                               <  2:1800116‑M1/93‑54 (MQ=255)

                                                            tttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcca                             >  1:2558605‑M1/1‑38 (MQ=255)

                                                            tttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcca                             >  2:1894352‑M1/1‑38 (MQ=255)

                                                            tttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcca                             >  1:2279420‑M1/1‑38 (MQ=255)

                                                            tttGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagcca                             <  1:1822823‑M1/93‑56 (MQ=255)

                                                              tGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccaca                           <  1:1034942‑M1/93‑58 (MQ=255)

                                                               gCCAGCCGTTGTGGAAATGATGATTACATCGTTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacag                          <  1:1785947‑M1/93‑59 (MQ=255)

                                                                 cAGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgaggtgcctggatcgggttagtaaagccacaggc                        >  2:1579965‑M1/1‑33 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggca                       <  2:2813559‑M1/93‑62 (MQ=255)

                                                                  aGCCGTTGTGGAAATGATGATTACATCGCTGCGCGGTAAATCGCCACAATTTCTTCGGCAGTTGCCTGGGTCGGGGTAGTAAAGCCACAGGCa                       >  2:2254097/1‑93 (MQ=255)

                                                                   gCCGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcat                      >  2:2698382‑M1/1‑31 (MQ=255)

                                                                    ccGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatc                     >  1:2496093‑M1/1‑30 (MQ=255)

                                                                    ccGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatc                     >  1:500904‑M1/1‑30 (MQ=255)

                                                                     cGTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatct                    <  1:1870333‑M1/93‑65 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                   <  2:826724‑M1/93‑66 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                   >  1:31443‑M1/1‑28 (MQ=255)

                                                                      gTTGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctt                   >  2:3552395‑M1/1‑28 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt                  >  2:2780172‑M1/1‑27 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt                  >  2:1930145‑M1/1‑27 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt                  >  1:2213599‑M1/1‑27 (MQ=255)

                                                                       ttGTGGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatcttt                  <  2:3433815‑M1/93‑67 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagttaagccacaggcatctttca                >  1:177062‑M1/1‑25 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttca                >  1:754509‑M1/1‑25 (MQ=255)

                                                                         gtgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttca                <  1:2182595‑M1/93‑69 (MQ=255)

                                                                         ggggaaATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttca                <  1:3677172‑M1/91‑69 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag               >  1:2298314‑M1/1‑24 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag               >  2:2055678‑M1/1‑24 (MQ=255)

                                                                          tgGAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcag               <  2:2413407‑M1/93‑70 (MQ=255)

                                                                            gAAATGATGATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcaggg             >  2:3977534‑M1/1‑22 (MQ=255)

                                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcat          <  1:2077888‑M1/93‑75 (MQ=255)

                                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcat          >  1:1503872‑M1/1‑19 (MQ=255)

                                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcat          >  1:1163335‑M1/1‑19 (MQ=255)

                                                                               atgatgATTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcat          >  2:961662‑M1/1‑19 (MQ=255)

                                                                                 gatgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcatta        <  2:3173025‑M1/93‑77 (MQ=255)

                                                                                 gatgatTACATCGCTGCGCGATAAATGGCCCAAATTTCTTCGTGAGTTGCCTGGACCGGGTTAGAAAACCCACGGGCATCTTTCAGGGCAttg        >  2:1372502/1‑92 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt      >  2:3394674‑M1/1‑15 (MQ=255)

                                                                                   tgatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagt      <  1:197064‑M1/93‑79 (MQ=255)

                                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtc     <  1:2866013‑M1/93‑80 (MQ=255)

                                                                                    gatTACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtc     >  2:2632921‑M1/1‑14 (MQ=255)

                                                                                       tACATCGCTGCgcgataaatcgccacaatttcttcgtgagttgcctggatcgggttagtaaagccacaggcatctttcagggcattagtcgcc  <  2:273795‑M1/93‑83 (MQ=255)

GACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACCTCGCTGC‑‑‑ATAAATCGCCACAATTTCTTCGTGAGTTGCCTGGATCGGGTTAGTAAAGCCACAGGCATCTTTCAGGGCATTAGTCGCC  >  NC_000913/3754220‑3754396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`