Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,792,026 A→G N138S (AAC→AGC)  rfaD → ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,792,0260AG99.6% 861.3 / NA 270N138S (AAC→AGC) rfaDADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (121/148);  minor base T (0/1);  total (121/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

AAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATT  >  NC_000913/3791936‑3792115
                                                                                          |                                                                                         
aaaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCg                                                                                         >  1:857523/1‑93 (MQ=255)
aaaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCg                                                                                         >  2:3232107/1‑93 (MQ=255)
 aaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGt                                                                                        <  1:2526794/93‑1 (MQ=255)
 aaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGt                                                                                        <  2:4517027/93‑1 (MQ=255)
  aTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTc                                                                                       >  2:1404955/1‑93 (MQ=255)
  aTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTc                                                                                       >  1:2968074/1‑93 (MQ=255)
   tCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCt                                                                                      <  2:3607858/93‑1 (MQ=255)
     ccGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTAc                                                                                    >  2:332904/1‑93 (MQ=255)
     ccGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTAc                                                                                    >  2:3517535/1‑93 (MQ=255)
     ccGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCACGCGAGTACGAAAAACCGTTGAGCGTCTAc                                                                                    >  1:2352278/1‑93 (MQ=255)
       gTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGGCTACgg                                                                                  >  1:185716/1‑93 (MQ=255)
       cttCCTGTACGCTTCTTCCGCAGCCACCTACGGCCGACGCTCCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACgg                                                                                  <  2:3734472/92‑1 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 <  2:2333991/93‑1 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 >  2:2146647/1‑93 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 >  1:3948808/1‑93 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 >  1:4067106/1‑93 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 <  1:1069647/93‑1 (MQ=255)
         tCCTGTGCGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGtt                                                                                <  2:689344/93‑1 (MQ=255)
         tCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGtt                                                                                >  1:76564/1‑93 (MQ=255)
          ccTGTACGCTGCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCAAGTACGAAAACCCGTTGAGCGTCTTCGGTTa                                                                               <  2:1150172/93‑1 (MQ=255)
            tGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACt                                                                             >  1:3242151/1‑93 (MQ=255)
             gTACGCTTCTTCCGCAGCCACCTACGGCGGACGCTCCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTc                                                                            <  2:3212013/93‑1 (MQ=255)
                cGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaa                                                                         >  1:4174106/1‑93 (MQ=255)
                 gCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaaa                                                                        <  1:2538976/93‑1 (MQ=255)
                 gCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaaa                                                                        <  1:1498471/93‑1 (MQ=255)
                 gCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaaa                                                                        <  2:2911689/93‑1 (MQ=255)
                   ttcttcCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAAtt                                                                      <  2:394877/93‑1 (MQ=255)
                       tcCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTg                                                                  <  1:421892/93‑1 (MQ=255)
                         cGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGtt                                                                <  1:479214/93‑1 (MQ=255)
                         cGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGtt                                                                <  2:4605010/93‑1 (MQ=255)
                          gCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGttt                                                               >  1:3442041/1‑93 (MQ=255)
                          gCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGttt                                                               <  1:56478/93‑1 (MQ=255)
                          gCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGttt                                                               <  2:585548/93‑1 (MQ=255)
                            aGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtga                                                             <  1:1240273/93‑1 (MQ=255)
                            aGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtga                                                             >  2:399914/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgat                                                            >  1:3825623/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgat                                                            >  2:2408834/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgat                                                            >  1:2662745/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAAACGTTGAGCGTCTACGGTTGCTCAAAATTCGTGgtttgt                                                            >  2:1760609/1‑90 (MQ=255)
                               cACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgatga                                                          <  2:1450354/93‑1 (MQ=255)
                                aCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGaa                                                         <  2:1210225/93‑1 (MQ=255)
                                aCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGaa                                                         <  2:2856251/93‑1 (MQ=255)
                                 ccTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAat                                                        >  2:1101275/1‑93 (MQ=255)
                                  cTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTATGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAata                                                       <  1:1753767/93‑1 (MQ=255)
                                  cTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAata                                                       >  2:2621052/1‑93 (MQ=255)
                                   tACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAatat                                                      >  2:3267870/1‑93 (MQ=255)
                                   tACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAatat                                                      <  2:2219999/93‑1 (MQ=255)
                                   tACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAatat                                                      <  2:799415/93‑1 (MQ=255)
                                      ggcggACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGtt                                                   <  2:847284/93‑1 (MQ=255)
                                       gcggACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTc                                                  <  1:2797549/93‑1 (MQ=255)
                                       gcggACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTc                                                  <  2:2100892/93‑1 (MQ=255)
                                          gACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTc                                               <  1:112368/93‑1 (MQ=255)
                                           aCGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCa                                              <  1:3081339/93‑1 (MQ=255)
                                             gCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCaaa                                            <  1:316735/93‑1 (MQ=255)
                                              cACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                           >  1:4582417/1‑93 (MQ=255)
                                              cACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                           <  2:3706222/93‑1 (MQ=255)
                                               aCCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATc                                          <  2:467329/93‑1 (MQ=255)
                                                ccTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcc                                         >  1:4183175/1‑93 (MQ=255)
                                                 cTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcct                                        >  1:1017000/1‑93 (MQ=255)
                                                  tCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctg                                       >  2:4165378/1‑93 (MQ=255)
                                                  tCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctg                                       >  2:1552515/1‑93 (MQ=255)
                                                    cGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctgcc                                     <  1:969834/93‑1 (MQ=255)
                                                     gACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctgcct                                    >  1:2174156/1‑93 (MQ=255)
                                                         ttATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAg                                >  2:1074505/1‑93 (MQ=255)
                                                            ttGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGa                             <  1:3923319/93‑1 (MQ=255)
                                                             tGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGaa                            <  1:1653097/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGCACt                          >  2:2435646/1‑93 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  2:1240605/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  2:4340682/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  1:1545627/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  2:2605640/93‑1 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  2:618644/1‑93 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  1:4535290/1‑93 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  1:3611612/1‑93 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  1:2059810/1‑93 (MQ=255)
                                                                 tCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCg                        >  2:402847/1‑93 (MQ=255)
                                                                 tCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCg                        >  2:3771966/1‑93 (MQ=255)
                                                                  cccGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGCATATGTTCGTCAAATCCTGCCTGCAGCGACCTCGc                       >  1:2300468/1‑93 (MQ=255)
                                                                   ccGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCa                      <  2:48008/93‑1 (MQ=255)
                                                                   ccGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCa                      <  1:1982164/93‑1 (MQ=255)
                                                                     gcgAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGa                    <  1:2902693/93‑1 (MQ=255)
                                                                      cgAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAt                   <  1:3792901/93‑1 (MQ=255)
                                                                      cgAGAACGAAAAACCGTTGATCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAt                   <  2:1896946/93‑1 (MQ=255)
                                                                       gAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAtt                  <  1:1711705/93‑1 (MQ=255)
                                                                        aGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttg                 >  1:2937088/1‑93 (MQ=255)
                                                                         gTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgt                <  1:1899368/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               <  2:3476621/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               <  2:540525/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               <  1:3366480/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               >  1:4644923/1‑93 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               >  2:2768383/1‑93 (MQ=255)
                                                                           aCGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgttg              <  1:3490123/93‑1 (MQ=255)
                                                                             gAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGc            <  1:1321814/93‑1 (MQ=255)
                                                                              aaaaaCCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCt           >  1:1336683/1‑93 (MQ=255)
                                                                                   ccGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGt      <  2:204559/93‑1 (MQ=255)
                                                                                     gTTGAGCGTCTGCGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTa    <  1:656165/93‑1 (MQ=255)
                                                                                     gTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTa    <  1:4471475/93‑1 (MQ=255)
                                                                                     gTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTa    >  1:1922125/1‑93 (MQ=255)
                                                                                       tGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTAtt  <  2:299768/93‑1 (MQ=255)
                                                                                       tGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTAtt  <  2:3835922/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATT  >  NC_000913/3791936‑3792115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑