BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,822,652	T→C	Y171H (TAC→CAC)	spoU →	putative RNA methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,822,652	0	T	C	100.0%	807.6 / NA	250	Y171H (TAC→CAC)	spoU	putative RNA methylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (115/135); total (115/135)

Only 100 of 253 total aligned reads displayed.

ATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA  >  NC_000913/3822562‑3822741

 tgatCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCAcc                                                                                        >  2:1827863/1‑93 (MQ=255)

 tgatCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGAAcc                                                                                        >  1:2445678/1‑93 (MQ=255)

    tCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGc                                                                                     >  2:1404470/1‑93 (MQ=255)

      ggCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGt                                                                                   <  2:1898316/93‑1 (MQ=255)

      ggCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGt                                                                                   >  2:2116935/1‑93 (MQ=255)

       gCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTg                                                                                  >  2:4049234/1‑93 (MQ=255)

       gCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTg                                                                                  >  2:4431645/1‑93 (MQ=255)

       gCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTg                                                                                  >  2:1574376/1‑93 (MQ=255)

         aTGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTGCGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGaa                                                                                <  2:2693112/93‑1 (MQ=255)

         aTGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGaa                                                                                >  1:59827/1‑93 (MQ=255)

         aTGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGaa                                                                                >  1:2121773/1‑93 (MQ=255)

           ggTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGaaaa                                                                              <  1:1157195/93‑1 (MQ=255)

            gTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAAc                                                                             <  2:3412443/93‑1 (MQ=255)

              gCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAg                                                                           >  1:4661100/1‑93 (MQ=255)

               cAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGc                                                                          <  1:4518260/93‑1 (MQ=255)

               cAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGc                                                                          <  1:37225/93‑1 (MQ=255)

               cAGTCGCTGAATGTTTCCGTTGCCTCAGCCCCCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGc                                                                          <  1:2168843/93‑1 (MQ=255)

                aGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCa                                                                         <  2:4084902/93‑1 (MQ=255)

                 gTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCAt                                                                        >  2:1774109/1‑93 (MQ=255)

                  tCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATg                                                                       <  2:3589136/93‑1 (MQ=255)

                   cGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGt                                                                      <  2:3768905/93‑1 (MQ=255)

                      tGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGc                                                                   >  1:1732957/1‑93 (MQ=255)

                      tGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGc                                                                   <  2:3463024/93‑1 (MQ=255)

                       taaTGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGcc                                                                  <  1:2768637/92‑1 (MQ=255)

                       gAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGcc                                                                  >  2:4194147/1‑93 (MQ=255)

                           gTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGaa                                                              <  2:3710877/93‑1 (MQ=255)

                           gTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGaa                                                              <  2:516829/93‑1 (MQ=255)

                           gTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGaa                                                              >  2:4306066/1‑93 (MQ=255)

                            tttCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAg                                                             >  1:1012142/1‑93 (MQ=255)

                              tCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCa                                                           <  2:787339/93‑1 (MQ=255)

                              tCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCa                                                           >  2:616963/1‑93 (MQ=255)

                                cGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCaga                                                         <  1:3227209/93‑1 (MQ=255)

                                 gTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCagag                                                        <  1:3550611/93‑1 (MQ=255)

                                  ttGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGc                                                       <  2:3598345/93‑1 (MQ=255)

                                    gCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaa                                                     >  1:1220422/1‑93 (MQ=255)

                                    gCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaa                                                     >  1:3374274/1‑93 (MQ=255)

                                     ccTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaac                                                    >  2:3754733/1‑93 (MQ=255)

                                     ccTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaac                                                    >  2:3172126/1‑93 (MQ=255)

                                      cTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaaca                                                   >  1:2940593/1‑93 (MQ=255)

                                      cTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaaca                                                   >  2:3207355/1‑93 (MQ=255)

                                       tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAACACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  >  2:2186044/1‑93 (MQ=255)

                                       tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  <  1:1836765/93‑1 (MQ=255)

                                       tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  >  2:2075481/1‑93 (MQ=255)

                                       tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  >  2:3722730/1‑93 (MQ=255)

                                       tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  >  2:577042/1‑93 (MQ=255)

                                       tCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGcaacaa                                                  >  1:714342/1‑93 (MQ=255)

                                        cAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCaacaac                                                 <  2:2361176/93‑1 (MQ=255)

                                        cAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCaacaac                                                 <  2:2165971/93‑1 (MQ=255)

                                          gCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGc                                               <  2:2908781/93‑1 (MQ=255)

                                          gCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGc                                               <  1:2417434/93‑1 (MQ=255)

                                          gCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGc                                               <  1:2519114/93‑1 (MQ=255)

                                           cccTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGcc                                              <  1:805375/93‑1 (MQ=255)

                                           cccTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGcc                                              >  1:3839632/1‑93 (MQ=255)

                                            ccTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCt                                             >  2:4377336/1‑93 (MQ=255)

                                             cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                            >  2:2948287/1‑93 (MQ=255)

                                             cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                            >  2:844328/1‑93 (MQ=255)

                                             cTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtg                                            <  1:3602132/93‑1 (MQ=255)

                                              tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                           >  1:976956/1‑93 (MQ=255)

                                              tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                           >  2:1390493/1‑93 (MQ=255)

                                              tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                           <  1:249456/93‑1 (MQ=255)

                                              tCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCACGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgt                                           <  1:1915089/93‑1 (MQ=255)

                                               cATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgtt                                          <  1:1054479/93‑1 (MQ=255)

                                                aTTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttg                                         >  1:1483167/1‑93 (MQ=255)

                                                aTTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAAAGCCtgttg                                         <  2:4571140/93‑1 (MQ=255)

                                                 ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttgt                                        >  1:3252477/1‑93 (MQ=255)

                                                 ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttgt                                        >  1:4039966/1‑93 (MQ=255)

                                                 ttCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCtgttgt                                        >  2:1075245/1‑93 (MQ=255)

                                                  tCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTgttgtt                                       <  1:3968577/93‑1 (MQ=255)

                                                   cTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGttt                                      <  1:3320545/93‑1 (MQ=255)

                                                   cTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGttt                                      <  2:1117235/93‑1 (MQ=255)

                                                   cTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTGGCCGGAAGCAGAGCAACAACGCCTGTTGttt                                      <  2:2553998/93‑1 (MQ=255)

                                                     ttACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGa                                    <  1:310180/93‑1 (MQ=255)

                                                      tACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGaa                                   <  1:65314/93‑1 (MQ=255)

                                                      tACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGaa                                   <  1:433096/93‑1 (MQ=255)

                                                       aCGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAg                                  <  1:2357665/93‑1 (MQ=255)

                                                        cGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAgg                                 <  2:560133/93‑1 (MQ=255)

                                                        cGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAgg                                 <  1:1576523/93‑1 (MQ=255)

                                                         gAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggc                                >  1:2656326/1‑93 (MQ=255)

                                                           aGCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcgg                              >  1:2017708/1‑93 (MQ=255)

                                                            gCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcggc                             <  2:3956210/93‑1 (MQ=255)

                                                            gCCCAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAggcggc                             >  1:1091420/1‑93 (MQ=255)

                                                             cccAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCt                            <  2:1687278/93‑1 (MQ=255)

                                                             cccAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCt                            >  2:1634166/1‑93 (MQ=255)

                                                              ccAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTa                           >  1:3093304/1‑93 (MQ=255)

                                                              ccAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTa                           >  2:1515318/1‑93 (MQ=255)

                                                              ccAGCGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTa                           >  1:441079/1‑93 (MQ=255)

                                                                  cGTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCg                       <  1:4166798/93‑1 (MQ=255)

                                                                   gTCAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCgg                      >  1:2196686/1‑93 (MQ=255)

                                                                     cAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTg                    <  1:4176049/93‑1 (MQ=255)

                                                                     cAGCGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTg                    <  2:2533491/93‑1 (MQ=255)

                                                                       gCGGCAAAATGCAGTCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCt                  <  1:3077182/93‑1 (MQ=255)

                                                                        cGGCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTg                 <  2:178529/93‑1 (MQ=255)

                                                                          gCAAAATGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGc               >  2:2801670/1‑93 (MQ=255)

                                                                            aaaaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGa             >  1:3719487/1‑93 (MQ=255)

                                                                             aaaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCCGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaa            >  2:944319/1‑93 (MQ=255)

                                                                              aaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaaa           <  1:2261265/93‑1 (MQ=255)

                                                                              aaTGCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGaaa           <  2:659222/93‑1 (MQ=255)

                                                                                 gCAGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTc        >  1:341258/1‑93 (MQ=255)

                                                                                   aGGCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGc      >  1:13094/1‑93 (MQ=255)

                                                                                    ggCATGCACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCa     >  1:3793619/1‑93 (MQ=255)

ATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAAGCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTTGTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAA  >  NC_000913/3822562‑3822741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`