Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,838,693 A→G I280T (ATC→ACC)  yicM ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,838,6930AG99.6% 744.3 / ‑5.2 236I280T (ATC→ACC) yicMputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (108/127);  total (109/127)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 240 total aligned reads displayed.

GTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGC  >  NC_000913/3838603‑3838782
                                                                                          |                                                                                         
gTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTg                                                                                         <  1:4284189/93‑1 (MQ=255)
  tAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGc                                                                                       <  2:3448023/93‑1 (MQ=255)
     gCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGat                                                                                    <  2:4339608/93‑1 (MQ=255)
      ccATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatc                                                                                   >  1:766710/1‑93 (MQ=255)
      ccATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatc                                                                                   <  1:1669204/93‑1 (MQ=255)
       cATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatca                                                                                  <  1:3529116/93‑1 (MQ=255)
       cATCCACGCCGAATCCCGCCAGGTTCATATACACCCGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatca                                                                                  >  2:860656/1‑93 (MQ=255)
        aTCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcat                                                                                 >  1:584904/1‑93 (MQ=255)
        aTCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcat                                                                                 >  2:214708/1‑93 (MQ=255)
        aTCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcat                                                                                 >  2:3874503/1‑93 (MQ=255)
         tCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcatc                                                                                <  2:1976062/93‑1 (MQ=255)
          ccACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATcc                                                                               <  1:4018647/93‑1 (MQ=255)
           cACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATccc                                                                              >  2:916965/1‑93 (MQ=255)
           cACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATccc                                                                              <  1:3237559/93‑1 (MQ=255)
              gCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGc                                                                           <  1:4658959/93‑1 (MQ=255)
                cGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCa                                                                         <  1:2097353/93‑1 (MQ=255)
                 gAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAt                                                                        >  2:770386/1‑93 (MQ=255)
                    acccGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATcac                                                                     <  1:315269/92‑1 (MQ=255)
                      ccGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCacac                                                                   >  2:1020753/1‑93 (MQ=255)
                       cGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACAcc                                                                  <  2:2281770/93‑1 (MQ=255)
                       cGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACAcc                                                                  <  1:3400618/93‑1 (MQ=255)
                         ccAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCg                                                                <  2:3209661/93‑1 (MQ=255)
                         ccAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCg                                                                >  1:1951330/1‑93 (MQ=255)
                          cAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCgg                                                               >  1:4522407/1‑93 (MQ=255)
                          cAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCgg                                                               >  2:681676/1‑93 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              >  1:1702270/1‑93 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              >  2:2240632/1‑93 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              <  1:2974833/93‑1 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCAGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              <  1:3122585/93‑1 (MQ=255)
                             gTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACg                                                            <  1:900798/93‑1 (MQ=255)
                             gTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACg                                                            >  2:4218848/1‑93 (MQ=255)
                              ttCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGt                                                           <  1:884063/93‑1 (MQ=255)
                              ttCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGt                                                           <  1:684138/93‑1 (MQ=255)
                               tCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGtt                                                          <  1:538252/93‑1 (MQ=255)
                               tCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGtt                                                          <  2:1474021/93‑1 (MQ=255)
                                 atatACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGt                                                        >  2:1808592/1‑93 (MQ=255)
                                  tataCACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGta                                                       >  2:142167/1‑93 (MQ=255)
                                   ataCACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtaa                                                      <  2:1275664/93‑1 (MQ=255)
                                    tccacCGGGCGAAAAAACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtagt                                                     <  2:3735865/91‑3 (MQ=255)
                                    taCACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtaat                                                     >  1:1598192/1‑93 (MQ=255)
                                      cacCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtaataa                                                   <  2:453832/93‑1 (MQ=255)
                                        ccGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGc                                                 <  1:2564572/93‑1 (MQ=255)
                                         cGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCg                                                >  2:2082966/1‑93 (MQ=255)
                                             cGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGaaa                                            <  1:1293844/93‑1 (MQ=255)
                                              gAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAg                                           <  2:2928177/93‑1 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         <  2:3919437/93‑1 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  1:2218194/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  1:1249347/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  2:614689/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  1:2721594/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         <  1:2761380/93‑1 (MQ=255)
                                                 tataCGTGAAGAAAGCAACCTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAt                                        <  2:477072/93‑1 (MQ=255)
                                                  ataCGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAtt                                       <  2:3088860/93‑1 (MQ=255)
                                                   taCGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAttt                                      >  2:57023/1‑93 (MQ=255)
                                                    aCGTGAAGAAAGCAAACTTCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAtttt                                     <  1:1146594/93‑1 (MQ=255)
                                                      gTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGt                                   >  1:1898654/1‑93 (MQ=255)
                                                       tGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGtt                                  >  1:3601551/1‑93 (MQ=255)
                                                        gaagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGttt                                 >  1:703817/1‑93 (MQ=255)
                                                        gaagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGttt                                 <  1:3653469/93‑1 (MQ=255)
                                                        gaagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGttt                                 >  1:4322796/1‑93 (MQ=255)
                                                         aagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTc                                <  1:4217672/93‑1 (MQ=255)
                                                          agaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCt                               <  2:3312395/93‑1 (MQ=255)
                                                           gaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTg                              >  1:2008700/1‑93 (MQ=255)
                                                            aaaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGa                             <  1:2890468/93‑1 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            <  2:3411303/93‑1 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            <  2:4141127/93‑1 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            >  1:659798/1‑93 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            <  2:2466194/93‑1 (MQ=255)
                                                              aGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATg                           >  2:2865764/1‑93 (MQ=255)
                                                               gCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGc                          >  1:1620540/1‑93 (MQ=255)
                                                                cAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCg                         <  1:4047923/93‑1 (MQ=255)
                                                                cAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCg                         >  2:3156222/1‑93 (MQ=255)
                                                                 aaaCTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGa                        >  2:444769/1‑93 (MQ=255)
                                                                    cTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAggg                     <  2:4531890/93‑1 (MQ=255)
                                                                     tGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGt                    <  1:4634539/93‑1 (MQ=255)
                                                                      gCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGtt                   <  1:2112050/93‑1 (MQ=255)
                                                                      gCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGtt                   >  1:1017590/1‑93 (MQ=255)
                                                                       cccGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTc                  <  1:1824559/93‑1 (MQ=255)
                                                                           gCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACGCCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCt              <  2:3644703/93‑1 (MQ=255)
                                                                             gAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTgg            <  2:2713122/93‑1 (MQ=255)
                                                                              aaaGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGc           >  1:1631661/1‑93 (MQ=255)
                                                                              aaaGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGc           >  1:4186168/1‑93 (MQ=255)
                                                                               aaGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCa          <  2:4270259/93‑1 (MQ=255)
                                                                               aaGAAATGAGGGTGGCGTCCATCCCTGCCATAACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCa          <  1:4429536/93‑1 (MQ=255)
                                                                                aGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAg         <  1:2071764/93‑1 (MQ=255)
                                                                                aGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAg         >  1:4494898/1‑93 (MQ=255)
                                                                                 gACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGGCGTTGTAATAAGCGGAAAGGATTTTGTTTCTGATGCGAGGGGTCCGCTGGGAGt        >  1:1799590/1‑93 (MQ=255)
                                                                                 gACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGt        >  1:1453167/1‑93 (MQ=255)
                                                                                   cATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGa      >  2:2025231/1‑93 (MQ=255)
                                                                                   cATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGCATTTTGATTCTGATGCGAGGGTTCGCCTGGCAGTGa      >  2:3022589/1‑93 (MQ=255)
                                                                                    aTGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGaa     >  2:1186655/1‑93 (MQ=255)
                                                                                    aTGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGaa     >  2:2615312/1‑93 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGCAAGTATTTTGTTTCTGATGCGAGGGCTCGCCTGGCAGTGAAg    >  2:4465491/1‑93 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAg    <  1:4155144/93‑1 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAg    >  2:2156934/1‑93 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAg    >  1:510280/1‑93 (MQ=255)
                                                                                      gAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAgg   <  2:3051625/93‑1 (MQ=255)
                                                                                      gAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAgg   <  1:2196084/93‑1 (MQ=255)
                                                                                      gAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAgg   >  2:3594971/1‑93 (MQ=255)
                                                                                       aaGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGc  <  1:3320790/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGC  >  NC_000913/3838603‑3838782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑