BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,851,757	T→C	V72A (GTG→GCG)	emrD →	2‑module integral membrane pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,851,757	0	T	C	100.0%	636.6 / NA	203	V72A (GTG→GCG)	emrD	2‑module integral membrane pump
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (125/78); total (125/78)

Only 100 of 214 total aligned reads displayed.

TCCGTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACGGTGTTGA  >  NC_000913/3851667‑3851846

tCCGTGAAGGGGCGGTGCAGCGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCgg                                                                                         >  1:3711286/1‑93 (MQ=255)

tCCGTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCgg                                                                                         >  1:4602354/1‑93 (MQ=255)

tCCGTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCgg                                                                                         <  1:3789674/93‑1 (MQ=255)

   gTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGgcc                                                                                      >  2:3500922/1‑93 (MQ=255)

   gTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGgcc                                                                                      <  2:448955/93‑1 (MQ=255)

   gTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGgcc                                                                                      <  2:406986/93‑1 (MQ=255)

    tGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACATACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGgccg                                                                                     >  1:2509322/1‑93 (MQ=255)

      aaGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGgccgcc                                                                                   >  2:4116121/1‑93 (MQ=255)

       aGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGccgccg                                                                                  >  2:1937550/1‑93 (MQ=255)

       aGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGccgccg                                                                                  >  2:230095/1‑93 (MQ=255)

        ggggCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGa                                                                                 >  2:310277/1‑93 (MQ=255)

        ggggCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGa                                                                                 >  1:1709176/1‑93 (MQ=255)

          ggCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGAcc                                                                               >  1:3200690/1‑93 (MQ=255)

           gCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCg                                                                              >  1:1988220/1‑93 (MQ=255)

            cGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCgg                                                                             <  1:1835799/93‑1 (MQ=255)

             ggTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGt                                                                            <  2:886156/93‑1 (MQ=255)

               tGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGa                                                                          <  2:3672054/93‑1 (MQ=255)

                gCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGAt                                                                         >  1:387386/1‑93 (MQ=255)

                gCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGAt                                                                         <  2:700583/93‑1 (MQ=255)

                  agagCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATcc                                                                       <  2:2510780/93‑1 (MQ=255)

                  agagCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATcc                                                                       >  2:3459459/1‑93 (MQ=255)

                   aggCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCt                                                                      <  1:600097/91‑1 (MQ=255)

                    agCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCtc                                                                     >  1:830082/1‑93 (MQ=255)

                       gTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCtcgtc                                                                  <  2:2953182/93‑1 (MQ=255)

                       gTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCACGCGGGCCGCCGACCTGTGATCCtcgtg                                                                  >  2:2146163/1‑92 (MQ=255)

                         aaTGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCgg                                                                >  1:1567181/1‑93 (MQ=255)

                           tGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGaa                                                              >  1:1316818/1‑93 (MQ=255)

                           tGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGaa                                                              >  2:1476094/1‑93 (MQ=255)

                            gggCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAAt                                                             <  2:3027906/93‑1 (MQ=255)

                            gggCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAAt                                                             <  2:713074/93‑1 (MQ=255)

                              gcgcTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGt                                                           <  1:4597861/93‑1 (MQ=255)

                                 cTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCa                                                        <  2:376012/93‑1 (MQ=255)

                                  ttATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAt                                                       >  1:764849/1‑93 (MQ=255)

                                  ttATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAt                                                       >  2:236070/1‑93 (MQ=255)

                                  tgttCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAt                                                       <  1:1204020/90‑1 (MQ=255)

                                   tATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAtt                                                      >  2:4633810/1‑93 (MQ=255)

                                   tATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAtt                                                      >  1:793471/1‑93 (MQ=255)

                                   tATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAtt                                                      <  1:2197359/93‑1 (MQ=255)

                                    aTCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAttt                                                     <  2:2021002/93‑1 (MQ=255)

                                     tCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAtttt                                                    >  1:3990314/1‑93 (MQ=255)

                                     tCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCAtttt                                                    >  2:2387566/1‑93 (MQ=255)

                                        gctgACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTAt                                                 <  1:4490919/93‑1 (MQ=255)

                                        gctgACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTAt                                                 <  2:37006/93‑1 (MQ=255)

                                          tgACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGc                                               >  2:2325115/1‑93 (MQ=255)

                                          tgACTTACCGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGGCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGc                                               <  1:1377345/93‑1 (MQ=255)

                                           gACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCt                                              <  1:734053/93‑1 (MQ=255)

                                           gACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCt                                              >  1:3539001/1‑93 (MQ=255)

                                            aCTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTg                                             >  2:2481316/1‑93 (MQ=255)

                                            aCTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTg                                             >  2:1707067/1‑93 (MQ=255)

                                            aCTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTg                                             >  2:3847409/1‑93 (MQ=255)

                                             cTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTgg                                            >  1:2012288/1‑93 (MQ=255)

                                              ttACGGTGTCTCACGGTTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGc                                           <  2:2697920/93‑1 (MQ=255)

                                              ttACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGc                                           >  2:1261847/1‑93 (MQ=255)

                                               tACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGGATGTCCATTTTTATGGTGGCa                                          >  2:560258/1‑93 (MQ=255)

                                               tACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCa                                          >  1:2112297/1‑93 (MQ=255)

                                                aCGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCaa                                         >  2:1320886/1‑93 (MQ=255)

                                                aCGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCaa                                         >  1:3333439/1‑93 (MQ=255)

                                                aCGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCaa                                         >  2:3540236/1‑93 (MQ=255)

                                                aCGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCaa                                         <  2:558849/93‑1 (MQ=255)

                                                 cGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc                                        <  2:3159611/93‑1 (MQ=255)

                                                 cGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAAc                                        >  1:230643/1‑93 (MQ=255)

                                                   gtgtCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACgg                                      >  1:4530831/1‑92 (MQ=255)

                                                   gtgtCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGc                                      >  1:519275/1‑93 (MQ=255)

                                                   gtgtCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGc                                      <  2:1405771/93‑1 (MQ=255)

                                                   gtgtCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGc                                      >  2:1845484/1‑93 (MQ=255)

                                                   gtgtCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGc                                      >  1:1123811/1‑93 (MQ=255)

                                                    tgtCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCt                                     >  1:554405/1‑93 (MQ=255)

                                                    tgtCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCt                                     <  1:2597414/93‑1 (MQ=255)

                                                      tctcACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGATGGCAACGCTgg                                   <  1:1440721/93‑1 (MQ=255)

                                                        tcACAGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTc                                 >  1:2728597/1‑93 (MQ=255)

                                                          ccaGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTcgc                               <  1:140476/92‑1 (MQ=255)

                                                          acaGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTcgc                               <  2:3969461/93‑1 (MQ=255)

                                                            aGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCCACGCTGGTCGCgg                             >  2:1016494/1‑93 (MQ=255)

                                                            aGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCgg                             <  2:43040/93‑1 (MQ=255)

                                                            aGCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCgg                             >  2:4262732/1‑93 (MQ=255)

                                                             gCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGt                            <  1:703529/93‑1 (MQ=255)

                                                             gCTGTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGGGATCCTCGTCGGAATGTCCATTTTTATTCTTGCAACGCTGGTCGCGGt                            >  2:1646151/1‑93 (MQ=255)

                                                                gTTTTATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCAc                         >  1:3097292/1‑93 (MQ=255)

                                                                 ttttATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACg                        <  1:1017438/93‑1 (MQ=255)

                                                                  tttATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGa                       <  2:2203958/93‑1 (MQ=255)

                                                                   ttATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGAc                      <  2:2416582/93‑1 (MQ=255)

                                                                    tATGGCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGAcc                     >  1:1784316/1‑93 (MQ=255)

                                                                      tGGCCCGATTTCCGACCGCGCGGGCCTCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTc                   <  2:3754307/93‑1 (MQ=255)

                                                                        gCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCa                 >  1:284373/1‑93 (MQ=255)

                                                                        gCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCa                 >  1:1599295/1‑93 (MQ=255)

                                                                        gCCCGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCa                 <  1:1908130/93‑1 (MQ=255)

                                                                          ccGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGt               >  1:2219631/1‑93 (MQ=255)

                                                                           cGATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGtt              <  1:3140023/93‑1 (MQ=255)

                                                                            gATTTCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGttt             >  2:2116942/1‑93 (MQ=255)

                                                                              tttCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGa           <  2:1517473/93‑1 (MQ=255)

                                                                              tttCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGa           >  2:2441140/1‑93 (MQ=255)

                                                                              tttCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGa           >  1:4204101/1‑93 (MQ=255)

                                                                               ttCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGAc          <  2:2576165/93‑1 (MQ=255)

                                                                                tCCGACCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACg         <  2:1057702/93‑1 (MQ=255)

                                                                                    gccGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACGgtgt     <  1:3908508/92‑1 (MQ=255)

                                                                                    aCCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACGgtgt     <  1:2447956/93‑1 (MQ=255)

                                                                                    aCCGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACGgtgt     >  2:2844456/1‑93 (MQ=255)

                                                                                     ccGCGCGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACGGTGtt    <  2:778274/93‑1 (MQ=255)

                                                                                       gcgcGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAGCGCTGGTCGCGGTCACGACCTCCAGTTTGACGGTGTTGa  <  1:3497106/93‑1 (MQ=255)

                                                                                       gcgcGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACGGTGTTGa  >  2:4570426/1‑93 (MQ=255)

TCCGTGAAGGGGCGGTGCAGAGCGTAATGGGCGCTTATCTGCTGACTTACGGTGTCTCACAGCTGTTTTATGGCCCGATTTCCGACCGCGTGGGCCGCCGACCGGTGATCCTCGTCGGAATGTCCATTTTTATGCTGGCAACGCTGGTCGCGGTCACGACCTCCAGTTTGACGGTGTTGA  >  NC_000913/3851667‑3851846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`