BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,875,855	G→A	N631N (AAC→AAT)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,875,855	0	G	A	99.3%	1062.8 / ‑5.6	297	N631N (AAC→AAT)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (152/143); total (153/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 312 total aligned reads displayed.

CTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGG  >  NC_000913/3875765‑3875944

 tCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTc                                                                                        <  1:838772/93‑1 (MQ=255)

  cGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCa                                                                                       >  1:486543/1‑93 (MQ=255)

   gAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCAc                                                                                      >  2:3119402/1‑93 (MQ=255)

    aaCAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCAcc                                                                                     >  2:2645157/1‑93 (MQ=255)

     aCAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCAccc                                                                                    >  1:3724781/1‑93 (MQ=255)

     aCAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCAccc                                                                                    >  2:3217227/1‑93 (MQ=255)

      cAGGTTTTGCTCAGCATTGGTGTGTACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCa                                                                                   >  2:4683790/1‑93 (MQ=255)

      cAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCa                                                                                   <  1:1695998/93‑1 (MQ=255)

        ggTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGc                                                                                 <  1:4170176/93‑1 (MQ=255)

         gTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCg                                                                                <  2:3331919/93‑1 (MQ=255)

          ttttGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCgg                                                                               <  2:1905215/93‑1 (MQ=255)

               cTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAAc                                                                          <  1:4006740/93‑1 (MQ=255)

                tCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACg                                                                         >  1:983800/1‑93 (MQ=255)

                  aGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGt                                                                       >  1:2186918/1‑93 (MQ=255)

                     aTTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTg                                                                    >  1:2660752/1‑93 (MQ=255)

                     aTTGGTGTGAACATCAAACTTCCACTGGCTGCCGAGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTCACGGTCTg                                                                    >  2:949410/1‑93 (MQ=255)

                      ttGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGc                                                                   >  2:1219141/1‑93 (MQ=255)

                      ttGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGc                                                                   >  1:3463846/1‑93 (MQ=255)

                         gtgtGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCtca                                                                >  2:787697/1‑93 (MQ=255)

                          tgtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCtcat                                                               >  2:4358390/1‑93 (MQ=255)

                          tgtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCtcat                                                               <  1:3018582/93‑1 (MQ=255)

                           gtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCtcatc                                                              >  1:612277/1‑93 (MQ=255)

                            tgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCtcatca                                                             >  2:2176185/1‑93 (MQ=255)

                             gAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAg                                                            >  2:2598020/1‑93 (MQ=255)

                               aCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGaa                                                          >  1:3078325/1‑93 (MQ=255)

                                cATCAAACTTCGTCTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGaaa                                                         <  2:2736259/93‑1 (MQ=255)

                                cATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGaaa                                                         <  1:319682/93‑1 (MQ=255)

                                 aTCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAg                                                        >  1:4399744/1‑93 (MQ=255)

                                 aTCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAg                                                        >  2:3701387/1‑93 (MQ=255)

                                 aTCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAg                                                        <  2:277107/93‑1 (MQ=255)

                                  tCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAgg                                                       <  1:753455/93‑1 (MQ=255)

                                  tCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAgg                                                       >  1:2730847/1‑93 (MQ=255)

                                   cAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGt                                                      <  1:302680/93‑1 (MQ=255)

                                   cAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGt                                                      <  2:3072278/93‑1 (MQ=255)

                                   cAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTAGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGt                                                      <  2:4661977/93‑1 (MQ=255)

                                    aaaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCCCCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTc                                                     <  1:1953456/93‑1 (MQ=255)

                                    aaaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTc                                                     <  1:2926687/93‑1 (MQ=255)

                                     aaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCa                                                    >  2:2683546/1‑93 (MQ=255)

                                     aaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCa                                                    <  2:4110451/93‑1 (MQ=255)

                                     aaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACAGTCTGCTCATCAGAAAGGTCa                                                    >  2:2059617/1‑93 (MQ=255)

                                      aCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAg                                                   <  2:1347742/93‑1 (MQ=255)

                                        ttCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCt                                                 <  1:687620/93‑1 (MQ=255)

                                         tCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCtt                                                <  2:3739803/93‑1 (MQ=255)

                                          ccACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCCCCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTc                                               <  2:4484652/93‑1 (MQ=255)

                                          ccACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTc                                               >  1:2559692/1‑93 (MQ=255)

                                          ccACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTc                                               >  1:2028246/1‑93 (MQ=255)

                                           cACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAAGGGTCTGCTCATCAGAAAGGTCAGCTTcc                                              >  2:3437072/1‑93 (MQ=255)

                                           cACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTcc                                              >  1:4485970/1‑93 (MQ=255)

                                            aCTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCg                                             >  1:2218551/1‑93 (MQ=255)

                                            aCTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCg                                             >  2:4641713/1‑93 (MQ=255)

                                             cTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGt                                            <  2:1851232/93‑1 (MQ=255)

                                                   gCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGt                                      >  1:675416/1‑93 (MQ=255)

                                                   gCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGt                                      <  2:2316604/93‑1 (MQ=255)

                                                   gCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGt                                      >  1:3801231/1‑93 (MQ=255)

                                                    ccGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTc                                     <  2:4226627/93‑1 (MQ=255)

                                                     cGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCg                                    <  2:2372864/93‑1 (MQ=255)

                                                     cGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCg                                    >  1:2351208/1‑93 (MQ=255)

                                                      gTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCgg                                   <  1:1460894/93‑1 (MQ=255)

                                                       tGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGc                                  >  2:676537/1‑93 (MQ=255)

                                                       tGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGc                                  >  1:1385936/1‑93 (MQ=255)

                                                        gCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCt                                 >  2:1959560/1‑93 (MQ=255)

                                                          tGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTga                               <  2:1710279/93‑1 (MQ=255)

                                                           gTTCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgat                              <  1:3611600/93‑1 (MQ=255)

                                                            ttCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                             <  2:1592516/93‑1 (MQ=255)

                                                            ttCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                             <  1:3771932/93‑1 (MQ=255)

                                                            ttCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                             <  2:3775079/93‑1 (MQ=255)

                                                            ttCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                             >  2:545730/1‑93 (MQ=255)

                                                            ttCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                             <  1:2971769/93‑1 (MQ=255)

                                                            ttCTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                             >  1:3908697/1‑93 (MQ=255)

                                                              cTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgataga                           <  2:3536206/93‑1 (MQ=255)

                                                              cTTTGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgataga                           <  1:4311592/93‑1 (MQ=255)

                                                               tttGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagat                          >  1:1023817/1‑93 (MQ=255)

                                                                ttGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagata                         <  1:1064561/93‑1 (MQ=255)

                                                                ttGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagata                         >  2:2137088/1‑93 (MQ=255)

                                                                ttGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagata                         <  1:1566164/93‑1 (MQ=255)

                                                                 tGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATaa                        <  2:2324787/93‑1 (MQ=255)

                                                                 tGTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATaa                        >  1:2486318/1‑93 (MQ=255)

                                                                  gTCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAg                       >  1:241744/1‑93 (MQ=255)

                                                                   tCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGc                      <  2:208520/93‑1 (MQ=255)

                                                                   tCGTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGc                      >  1:1262101/1‑93 (MQ=255)

                                                                     gTTCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGctc                    <  2:2829397/93‑1 (MQ=255)

                                                                      ttCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGctct                   >  2:17555/1‑93 (MQ=255)

                                                                      ttCAGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGctct                   >  1:4561275/1‑93 (MQ=255)

                                                                       tCAGTTCGCGGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCtt                  <  1:3862137/93‑1 (MQ=255)

                                                                        taGTTCGCTGCCCAGCGCATTC‑GCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTc                <  2:3183427/92‑1 (MQ=255)

                                                                         aGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTc                <  2:1331170/93‑1 (MQ=255)

                                                                         aGTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTc                <  1:1808042/93‑1 (MQ=255)

                                                                          gTTCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCa               >  2:2901151/1‑93 (MQ=255)

                                                                           ttCGCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAg              >  2:741521/1‑93 (MQ=255)

                                                                             cGCTGACCAGCGCATTCTCCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCa            <  1:1218473/93‑1 (MQ=255)

                                                                              gCTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCAt           >  2:4393993/1‑93 (MQ=255)

                                                                               cTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCAtt          >  1:337644/1‑93 (MQ=255)

                                                                               cTGACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCAtt          <  1:436475/93‑1 (MQ=255)

                                                                                 gACCAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGc        <  2:1083824/93‑1 (MQ=255)

                                                                                  aCCAGCGCATTCAACCAGCGGGGAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGCCTGATATATAAGCTCTTTCAGCATTGCt       >  2:4052767/1‑93 (MQ=255)

                                                                                   ccAGCGCATTCCCCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTGCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCtt      <  1:2510153/93‑1 (MQ=255)

                                                                                   ccAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCtt      >  1:54894/1‑93 (MQ=255)

                                                                                    cAGCGCATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCttt     <  1:3968050/93‑1 (MQ=255)

                                                                                      gcgcATTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCg   >  2:3905196/1‑93 (MQ=255)

                                                                                       cgcaTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCgg  <  2:147215/93‑1 (MQ=255)

CTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGG  >  NC_000913/3875765‑3875944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`