BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	413,698	T→C	H427R (CAC→CGC)	sbcC ←	ATP‑dependent dsDNA exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	413,698	0	T	C	100.0%	539.9 / NA	173	H427R (CAC→CGC)	sbcC	ATP‑dependent dsDNA exonuclease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (75/98); total (75/98)

Only 100 of 180 total aligned reads displayed.

GTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGTGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCA  >  NC_000913/413608‑413787

gTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGgcgc                                                                                         >  2:338302/1‑93 (MQ=255)

gTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGgcgc                                                                                         <  1:4211370/93‑1 (MQ=255)

gTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGgcgc                                                                                         >  1:13190/1‑93 (MQ=255)

 tCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCt                                                                                        <  1:3122476/93‑1 (MQ=255)

 tCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCt                                                                                        <  2:659764/93‑1 (MQ=255)

 tCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCt                                                                                        <  2:4085045/93‑1 (MQ=255)

   tGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGa                                                                                      <  1:1362191/93‑1 (MQ=255)

    gTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGAc                                                                                     >  2:2576072/1‑93 (MQ=255)

    gTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGAc                                                                                     <  2:601354/93‑1 (MQ=255)

    gTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGAc                                                                                     >  2:1482303/1‑93 (MQ=255)

     ttCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACg                                                                                    <  2:711130/93‑1 (MQ=255)

      tCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCCACCAGGCGCTGACGc                                                                                   >  2:4439455/1‑93 (MQ=255)

       cTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCa                                                                                  >  1:2408422/1‑93 (MQ=255)

       cTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCa                                                                                  >  1:844467/1‑93 (MQ=255)

         tGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGt                                                                                <  1:2763133/93‑1 (MQ=255)

           cGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTgg                                                                              <  2:2900098/93‑1 (MQ=255)

           cGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTgg                                                                              <  2:3559389/93‑1 (MQ=255)

            gTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTggg                                                                             <  2:323789/93‑1 (MQ=255)

            gTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTggg                                                                             <  1:4079846/93‑1 (MQ=255)

            gTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTggg                                                                             <  2:1631171/93‑1 (MQ=255)

            gTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTggg                                                                             <  2:1561063/93‑1 (MQ=255)

            gTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTggg                                                                             >  1:4080824/1‑93 (MQ=255)

              gACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCg                                                                           <  1:248210/93‑1 (MQ=255)

                 aTTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGTGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGCGCGTTg                                                                        <  2:3899402/93‑1 (MQ=255)

                  ttCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGc                                                                       <  2:1676965/93‑1 (MQ=255)

                  ttCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGc                                                                       >  1:278022/1‑93 (MQ=255)

                  ttCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGc                                                                       <  2:2319800/93‑1 (MQ=255)

                   tCTGGATAGCGACCTCTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCt                                                                      <  1:746748/93‑1 (MQ=255)

                     tGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCa                                                                    >  1:77914/1‑93 (MQ=255)

                      ggATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAg                                                                   <  2:4634967/93‑1 (MQ=255)

                       gagaGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGc                                                                  <  1:888580/90‑1 (MQ=255)

                        aTAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCa                                                                 <  1:4462064/93‑1 (MQ=255)

                         tAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCAt                                                                >  1:4610140/1‑93 (MQ=255)

                          aGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATg                                                               <  2:2257937/93‑1 (MQ=255)

                           gCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGt                                                              <  1:1900372/93‑1 (MQ=255)

                           gCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGt                                                              <  1:4186891/93‑1 (MQ=255)

                            cGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGtt                                                             <  1:4200867/93‑1 (MQ=255)

                             gACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTg                                                            >  1:296536/1‑93 (MQ=255)

                                cTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTgcgc                                                         >  1:10987/1‑93 (MQ=255)

                                cTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTgcgc                                                         <  2:1283300/93‑1 (MQ=255)

                                cTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTgcgc                                                         >  1:10874/1‑93 (MQ=255)

                                cTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTgcgc                                                         >  2:1930348/1‑93 (MQ=255)

                                 tGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGcc                                                        <  1:497499/93‑1 (MQ=255)

                                 tGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCAAGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGcc                                                        >  1:2510107/1‑93 (MQ=255)

                                   tAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAg                                                      >  1:613439/1‑93 (MQ=255)

                                    aaCTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAgg                                                     <  2:1544610/93‑1 (MQ=255)

                                    aaCTGCGCCAGACGTTTTTGCTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAgg                                                     <  2:3952319/93‑1 (MQ=255)

                                        gcgcCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCgg                                                 >  1:4326805/1‑93 (MQ=255)

                                        gcgcCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCgg                                                 <  2:626834/93‑1 (MQ=255)

                                         cgcCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGt                                                <  2:1132928/93‑1 (MQ=255)

                                          gcCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTa                                               >  2:4301073/1‑93 (MQ=255)

                                          cccAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTa                                               <  2:1336770/92‑1 (MQ=255)

                                            cAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGc                                             <  1:2194302/93‑1 (MQ=255)

                                             aGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCa                                            >  2:1942375/1‑93 (MQ=255)

                                              gACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCaa                                           >  2:1048336/1‑93 (MQ=255)

                                              gACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCaa                                           >  1:1026886/1‑93 (MQ=255)

                                              gACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCaa                                           >  1:3105838/1‑93 (MQ=255)

                                              gACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCaa                                           <  1:2325840/93‑1 (MQ=255)

                                              gACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCaa                                           >  1:1932651/1‑93 (MQ=255)

                                               aCGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAAc                                          <  1:3271572/93‑1 (MQ=255)

                                               aCGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAAc                                          >  2:1248041/1‑93 (MQ=255)

                                                 gTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACtt                                        >  1:1577967/1‑93 (MQ=255)

                                                  tttttGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTc                                       >  1:527417/1‑93 (MQ=255)

                                                   ttttGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCa                                      <  2:451383/93‑1 (MQ=255)

                                                     ttgttgGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATc                                    <  2:1862570/93‑1 (MQ=255)

                                                     ttgttgGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATc                                    <  1:3202582/93‑1 (MQ=255)

                                                      tgttgGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATcg                                   <  2:1997920/93‑1 (MQ=255)

                                                        ttgGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATcggc                                 <  2:3004262/93‑1 (MQ=255)

                                                        ttgGGGAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATcggc                                 <  2:2364712/93‑1 (MQ=255)

                                                          ggggAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATcggcgg                               >  2:4378852/1‑93 (MQ=255)

                                                          ggggAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATcggcgg                               <  1:4342345/93‑1 (MQ=255)

                                                           gggAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGt                              >  1:1023447/1‑93 (MQ=255)

                                                            ggAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGtt                             <  2:2470073/93‑1 (MQ=255)

                                                             gAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTa                            <  1:2575441/93‑1 (MQ=255)

                                                             gAACAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTa                            <  1:4014708/93‑1 (MQ=255)

                                                              aaCAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTaa                           <  1:3724026/93‑1 (MQ=255)

                                                               aCAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCTGTAGCAACTTCATCGGCGGTTAAc                          >  2:2143158/1‑93 (MQ=255)

                                                               aCAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAAc                          >  1:1742926/1‑93 (MQ=255)

                                                               aCAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAAc                          <  1:2642867/93‑1 (MQ=255)

                                                               aCAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAAc                          >  1:3486610/1‑93 (MQ=255)

                                                               aCAATCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAAc                          <  1:2007083/93‑1 (MQ=255)

                                                                 aaTCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACgc                        >  1:2160440/1‑92 (MQ=255)

                                                                 aaTCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGt                        >  1:1221480/1‑93 (MQ=255)

                                                                  aTCTGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTc                       >  1:4592415/1‑93 (MQ=255)

                                                                  aTCTGTCCATGCAGCGCGACCAGGCGCTGAAGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTc                       >  2:3993775/1‑93 (MQ=255)

                                                                     tGTCCATGCAGCGCGTGCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAAc                    <  1:1568410/93‑1 (MQ=255)

                                                                     tGTCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAAc                    >  1:1239753/1‑93 (MQ=255)

                                                                       tCCATGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGt                  >  1:3599179/1‑93 (MQ=255)

                                                                          aTGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGAt               >  1:3848236/1‑93 (MQ=255)

                                                                          aTGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGAt               <  1:287795/93‑1 (MQ=255)

                                                                           tGCAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGGGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATc              <  2:917175/93‑1 (MQ=255)

                                                                             cAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATcgc            >  2:383477/1‑93 (MQ=255)

                                                                             cAGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATcgc            <  1:706876/93‑1 (MQ=255)

                                                                              aGCGCGACTAGGCGCTGGCGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATcgcc           <  1:176399/93‑1 (MQ=255)

                                                                              aGCGCGACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATcgcc           >  1:2476515/1‑93 (MQ=255)

                                                                                  cgtccAGGCGCGGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGTGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCaa       <  1:3789828/90‑1 (MQ=255)

                                                                                  cgACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCaa       <  1:2250034/93‑1 (MQ=255)

                                                                                   gACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTTACGTCAACGTGATCGCCGCAAg      >  2:411574/1‑93 (MQ=255)

                                                                                   gACCAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAg      >  2:3131929/1‑93 (MQ=255)

                                                                                     ccAGGCGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAgcg    >  1:3074038/1‑93 (MQ=255)

GTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGCGCGACCAGGTGCTGACGCAGTGGGCGTTGCTCAGCATGTTGCGCCAGGGCGGTAGCAACTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCA  >  NC_000913/413608‑413787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`