BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,917,055	T→C	Q144R (CAG→CGG)	atpA ←	membrane‑bound ATP synthase, F1 sector, alpha‑subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,917,055	0	T	C	100.0%	750.7 / NA	236	Q144R (CAG→CGG)	atpA	membrane‑bound ATP synthase, F1 sector, alpha‑subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (127/109); total (127/109)

Only 100 of 251 total aligned reads displayed.

CCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATT  >  NC_000913/3916965‑3917144

 cTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGAt                                                                                        <  2:927597/93‑1 (MQ=255)

 cTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGAt                                                                                        >  2:3558863/1‑93 (MQ=255)

  tGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATc                                                                                       <  2:3423107/93‑1 (MQ=255)

  tGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATc                                                                                       >  1:844423/1‑93 (MQ=255)

    tCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTa                                                                                     <  2:1917927/93‑1 (MQ=255)

     cTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTAc                                                                                    <  1:1292674/93‑1 (MQ=255)

       gACGGTCGCCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACgg                                                                                  <  2:2878531/93‑1 (MQ=255)

       gACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACgg                                                                                  >  1:1670549/1‑93 (MQ=255)

       gACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACgg                                                                                  >  1:3643783/1‑93 (MQ=255)

         cGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGAc                                                                                <  2:660338/93‑1 (MQ=255)

            tCACCGATTACCAAGTCACGCTGACCACGACCGATTGGGATCATGGAGTCATCGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGa                                                                             <  2:2602416/93‑1 (MQ=255)

             cACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGAc                                                                            >  2:2851147/1‑93 (MQ=255)

               ccGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGt                                                                          <  1:2782030/93‑1 (MQ=255)

                cGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGtt                                                                         >  2:1150360/1‑93 (MQ=255)

                 gatgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTc                                                                        >  1:1156255/1‑93 (MQ=255)

                 gatgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTc                                                                        <  1:685782/93‑1 (MQ=255)

                 gatgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTc                                                                        <  1:2647800/93‑1 (MQ=255)

                 gatgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTc                                                                        >  1:1460620/1‑93 (MQ=255)

                  atgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCg                                                                       >  2:1055297/1‑93 (MQ=255)

                     atCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTAAACGGCTTTATAACCGGTCTGTACCGGCCGATCTCCGGAATGACGTCCGATa                                                                    >  2:3851942/1‑93 (MQ=255)

                      tCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATaa                                                                   <  2:3711114/93‑1 (MQ=255)

                      tCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATaa                                                                   >  1:2313048/1‑93 (MQ=255)

                       cAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAAc                                                                  <  2:3455406/93‑1 (MQ=255)

                       cAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAAc                                                                  <  2:2430807/93‑1 (MQ=255)

                         aTTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGc                                                                >  1:2937558/1‑93 (MQ=255)

                          ttCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGcc                                                               <  1:3706202/93‑1 (MQ=255)

                          ttCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGAGAACGcc                                                               >  1:957631/1‑93 (MQ=255)

                           tCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGccc                                                              >  2:1615137/1‑93 (MQ=255)

                           tCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGccc                                                              >  1:2632417/1‑93 (MQ=255)

                           tCACGCTGACAACGACTGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCGGTACCGGCCGATCTACGGACTGACGTTCGATAACGccc                                                              <  1:4276555/93‑1 (MQ=255)

                            cACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCg                                                             >  2:1239017/1‑93 (MQ=255)

                              cGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGa                                                           >  1:816943/1‑93 (MQ=255)

                              cGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGa                                                           <  1:2478901/93‑1 (MQ=255)

                                cTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGc                                                         <  2:4321954/93‑1 (MQ=255)

                                  gACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGa                                                       >  1:3865026/1‑93 (MQ=255)

                                   aCCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGAt                                                      <  2:2359792/93‑1 (MQ=255)

                                   aCCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGAt                                                      <  2:1324997/93‑1 (MQ=255)

                                    ccACTACCGATTGGGATCATGCAGTCACCGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGAtt                                                     <  1:90750/93‑1 (MQ=255)

                                    ccACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGAtt                                                     >  2:1708720/1‑93 (MQ=255)

                                     cACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTg                                                    >  1:2720843/1‑93 (MQ=255)

                                     cACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTg                                                    >  1:2575804/1‑93 (MQ=255)

                                      aCGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGc                                                   >  2:1422013/1‑93 (MQ=255)

                                         aCCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTc                                                <  1:187642/93‑1 (MQ=255)

                                           cGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTa                                              <  1:1088225/93‑1 (MQ=255)

                                            gATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATACCGCCCGGAGCGATTGCTTCTAc                                             >  2:402153/1‑93 (MQ=255)

                                            gATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAc                                             >  2:1382587/1‑93 (MQ=255)

                                             aTTGGGATCATGGAGTCAACGGTTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAca                                            <  1:4131979/93‑1 (MQ=255)

                                             aTTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATGACGCCCGGAGCGATAGTTTCTAca                                            >  2:1800676/1‑93 (MQ=255)

                                             aTTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAca                                            >  2:395337/1‑93 (MQ=255)

                                              ttGGCATCATGGAGTCAACGCCTTTATACCCGGTCTGTACCGCCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcag                                           <  2:3252523/93‑1 (MQ=255)

                                               tGGTATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcagc                                          <  2:144873/93‑1 (MQ=255)

                                               tGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcagc                                          >  1:1443478/1‑93 (MQ=255)

                                                  gATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCaga                                       >  1:4558455/1‑93 (MQ=255)

                                                    tCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAgaga                                     <  2:3518108/93‑1 (MQ=255)

                                                    tCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAgaga                                     >  2:3639378/1‑93 (MQ=255)

                                                    tCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAgaga                                     >  2:3826459/1‑93 (MQ=255)

                                                    tCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAgaga                                     <  2:2197000/93‑1 (MQ=255)

                                                     cATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGaa                                    <  1:1699174/93‑1 (MQ=255)

                                                     cATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGaa                                    <  2:2104499/93‑1 (MQ=255)

                                                      aTGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAg                                   >  1:3294166/1‑93 (MQ=255)

                                                           gTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCcgtc                              >  2:4259600/1‑93 (MQ=255)

                                                             cAACGACTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCcgtcgt                            <  2:572323/93‑1 (MQ=255)

                                                              aaCGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTg                           >  2:1268123/1‑93 (MQ=255)

                                                               aCGTCTTTATACCCGTTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGa                          <  2:417607/93‑1 (MQ=255)

                                                               aCGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGa                          >  1:4476368/1‑93 (MQ=255)

                                                                cGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGAt                         >  2:4440339/1‑93 (MQ=255)

                                                                 ggCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATc                        >  1:1576156/1‑93 (MQ=255)

                                                                  gCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATcc                       >  1:4215523/1‑93 (MQ=255)

                                                                  gCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATcc                       >  2:477598/1‑93 (MQ=255)

                                                                   cTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCa                      <  2:436667/93‑1 (MQ=255)

                                                                    tttATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAg                     >  1:1860007/1‑93 (MQ=255)

                                                                    tttATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAg                     >  2:1580347/1‑93 (MQ=255)

                                                                    tttATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAACCGTCGTGATCCAg                     >  2:3105482/1‑93 (MQ=255)

                                                                     ttATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGc                    >  1:3224628/1‑93 (MQ=255)

                                                                        taACCGGTCTGTACCGGTCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAACCGGCGTGATCCAGCGGa                 >  1:4399521/1‑93 (MQ=255)

                                                                        taACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGa                 >  2:275863/1‑93 (MQ=255)

                                                                        taACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGa                 >  1:2197206/1‑93 (MQ=255)

                                                                        taACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGa                 <  2:1891571/93‑1 (MQ=255)

                                                                         aaCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGAc                <  1:129910/93‑1 (MQ=255)

                                                                          aCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGAcc               >  2:2743123/1‑93 (MQ=255)

                                                                          aCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGAcc               <  2:1106128/93‑1 (MQ=255)

                                                                           ccGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCt              <  2:4222019/93‑1 (MQ=255)

                                                                           ccGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCt              >  2:412280/1‑93 (MQ=255)

                                                                            cGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCtt             <  1:2918885/93‑1 (MQ=255)

                                                                             ggTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCttt            <  1:4321849/93‑1 (MQ=255)

                                                                              gTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTa           >  2:3137634/1‑93 (MQ=255)

                                                                               tCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTAc          <  2:3340966/93‑1 (MQ=255)

                                                                               tCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTAc          <  2:2530691/93‑1 (MQ=255)

                                                                                cTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTAcc         >  1:2871320/1‑93 (MQ=255)

                                                                                cTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTAcc         >  2:3684047/1‑93 (MQ=255)

                                                                                 tGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCg        >  1:1328427/1‑93 (MQ=255)

                                                                                 tGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCg        >  2:3534854/1‑93 (MQ=255)

                                                                                  gTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGt       >  1:4430097/1‑93 (MQ=255)

                                                                                  gTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGt       <  1:539522/93‑1 (MQ=255)

                                                                                    aCCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCg     <  1:3112890/93‑1 (MQ=255)

                                                                                    aCCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCg     >  1:755486/1‑93 (MQ=255)

                                                                                     ccGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGa    >  2:328440/1‑93 (MQ=255)

                                                                                     ccGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGa    <  1:3345994/93‑1 (MQ=255)

                                                                                     ccGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGa    <  1:2017958/93‑1 (MQ=255)

                                                                                      cGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGAt   >  2:868038/1‑93 (MQ=255)

CCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATT  >  NC_000913/3916965‑3917144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`