BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,742	T→C	A320A (GCT→GCC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,742	0	T	C	99.5%	667.7 / NA	213	A320A (GCT→GCC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (121/91); minor base A (1/0); total (122/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

cATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgc                                                                                         <  2:2274539/93‑1 (MQ=255)

cATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgc                                                                                         >  2:555380/1‑93 (MQ=255)

 aTCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAgccgct                                                                                        >  1:2333268/1‑93 (MQ=255)

   cggcgggtgTATCCGCCAGTCTCGTTTGACTATGCTTCTTCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTTGAGTATGGCCAGCCGCTGt                                                                                      <  2:4187060/93‑1 (MQ=255)

   cggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTACAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGt                                                                                      >  2:4393841/1‑93 (MQ=255)

     gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    <  1:459600/93‑1 (MQ=255)

       ggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgc                                                                                  <  1:3624134/93‑1 (MQ=255)

        gcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTcgcg                                                                                 >  1:2676735/1‑93 (MQ=255)

         cggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCga                                                                                <  2:181687/93‑1 (MQ=255)

           gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGGATGGCCAGCCGCTGTTCGCgaga                                                                              >  1:1635185/1‑93 (MQ=255)

            tATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGagag                                                                             <  1:1479295/93‑1 (MQ=255)

            tATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCAAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGagag                                                                             <  2:4628519/93‑1 (MQ=255)

             aTCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGc                                                                            >  2:4387802/1‑93 (MQ=255)

             aTCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGc                                                                            >  2:3380891/1‑93 (MQ=255)

              tCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCg                                                                           >  2:2891784/1‑93 (MQ=255)

                 gCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTcc                                                                        >  2:1960872/1‑93 (MQ=255)

                   cAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCt                                                                      >  2:3139864/1‑93 (MQ=255)

                   cAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCt                                                                      >  2:1468611/1‑93 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  1:513078/93‑1 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    >  1:4535363/1‑93 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  1:2299934/93‑1 (MQ=255)

                       ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtctc                                                                  <  2:3875077/93‑1 (MQ=255)

                        tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTctct                                                                 >  2:2825961/1‑93 (MQ=255)

                           tttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgct                                                              <  2:5894/93‑1 (MQ=255)

                           tttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgct                                                              >  1:3255846/1‑93 (MQ=255)

                                cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaat                                                         <  2:2298021/93‑1 (MQ=255)

                                cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaat                                                         >  1:1032508/1‑93 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        >  1:3913/1‑93 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        >  1:1450375/1‑93 (MQ=255)

                                  aTGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaataa                                                       <  2:1621419/93‑1 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:3331469/1‑93 (MQ=255)

                                     ctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAttt                                                    <  2:2567152/93‑1 (MQ=255)

                                     ctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAttt                                                    <  2:913336/93‑1 (MQ=255)

                                      tgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   <  2:4296736/93‑1 (MQ=255)

                                      tgctgctgcAACTGCCGCATAGCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   <  1:3180949/93‑1 (MQ=255)

                                       gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTAt                                                  >  2:3451216/1‑93 (MQ=255)

                                       gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTAt                                                  <  1:2243429/93‑1 (MQ=255)

                                        ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                 <  2:3247821/93‑1 (MQ=255)

                                        ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                 <  2:1355259/93‑1 (MQ=255)

                                        ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                 <  1:2867565/93‑1 (MQ=255)

                                        ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                 <  1:2126151/93‑1 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                >  2:277197/1‑93 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                >  2:870874/1‑93 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                >  1:928521/1‑93 (MQ=255)

                                            tgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgccg                                             >  1:1274160/1‑93 (MQ=255)

                                              cAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCgccgcc                                           <  2:1208434/93‑1 (MQ=255)

                                               aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCa                                          >  1:3017010/1‑93 (MQ=255)

                                               aaCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCa                                          <  1:780015/93‑1 (MQ=255)

                                                aCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAg                                         <  2:2417313/93‑1 (MQ=255)

                                                aCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCAGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAg                                         >  1:2521337/1‑93 (MQ=255)

                                                  tGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCg                                       >  2:3418414/1‑93 (MQ=255)

                                                   gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGt                                      >  2:890847/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   >  1:1620880/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   >  2:1501933/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   >  2:2628797/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   <  1:1137836/93‑1 (MQ=255)

                                                       cATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGa                                  <  1:3442150/93‑1 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 >  2:4570897/1‑93 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 <  1:2900259/93‑1 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  2:4114342/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  1:2505351/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  1:4251654/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  1:3107627/1‑93 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                <  1:355400/93‑1 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  2:1701877/1‑93 (MQ=255)

                                                          atCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                               <  2:1382118/93‑1 (MQ=255)

                                                           tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAg                              <  2:1644191/93‑1 (MQ=255)

                                                           tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAg                              >  2:3172188/1‑93 (MQ=255)

                                                              gCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                           >  2:3572519/1‑93 (MQ=255)

                                                              gCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCCCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcg                           >  1:2180786/1‑93 (MQ=255)

                                                               cgaGGTTCAGTGTGGAGTATGCCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcgg                          <  2:3231496/91‑1 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  2:474533/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  2:2181467/1‑93 (MQ=255)

                                                                   gTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTc                      >  2:2511441/1‑93 (MQ=255)

                                                                     tCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGc                    <  1:3549126/93‑1 (MQ=255)

                                                                      cAGTGTGGATTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  1:4194259/93‑1 (MQ=255)

                                                                      cAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCa                   <  2:2796946/93‑1 (MQ=255)

                                                                        gtgtGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATc                 >  1:4655288/1‑93 (MQ=255)

                                                                        gtgtGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATc                 <  1:1387914/93‑1 (MQ=255)

                                                                        gtgtGGAGTATGCCCAGCCGCTGTTCCCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATc                 <  2:1069353/93‑1 (MQ=255)

                                                                         tgtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                <  1:3926485/93‑1 (MQ=255)

                                                                           tgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg              >  2:3667616/1‑93 (MQ=255)

                                                                           tgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg              >  1:1531244/1‑93 (MQ=255)

                                                                              ggTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa           <  2:657075/92‑1 (MQ=255)

                                                                              aGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa           <  2:2951320/93‑1 (MQ=255)

                                                                              aGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa           <  1:1976952/93‑1 (MQ=255)

                                                                              aGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa           <  1:3792338/93‑1 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          >  1:4111139/1‑93 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          >  1:731969/1‑93 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGGAt          >  1:1852646/1‑93 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       >  2:1738066/1‑93 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       <  2:2728812/93‑1 (MQ=255)

                                                                                   ggCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaaa      <  1:3359585/93‑1 (MQ=255)

                                                                                    gCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAg     <  1:1941872/93‑1 (MQ=255)

                                                                                    gCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAg     >  1:2382755/1‑93 (MQ=255)

                                                                                     ccAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgc    <  1:2495182/93‑1 (MQ=255)

                                                                                      cAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg   >  1:1144257/1‑93 (MQ=255)

                                                                                       aGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc  >  2:3773242/1‑93 (MQ=255)

                                                                                       aGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc  <  2:2664593/93‑1 (MQ=255)

                                                                                       aGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc  >  2:2673377/1‑93 (MQ=255)

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`