Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,929,618 A→G T226A (ACG→GCG)  kup → low affinity potassium transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,929,6180AG99.1% 669.3 / ‑3.3 216T226A (ACG→GCG) kuplow affinity potassium transport system
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (122/92);  total (124/92)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 224 total aligned reads displayed.

CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTG  >  NC_000913/3929528‑3929707
                                                                                          |                                                                                         
 aTGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgg                                                                                        <  2:4098243/93‑1 (MQ=255)
 aTGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgg                                                                                        >  1:704289/1‑93 (MQ=255)
  tGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCggg                                                                                       >  1:3685723/1‑93 (MQ=255)
   gcgcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgggg                                                                                      >  2:3809431/1‑93 (MQ=255)
     gcTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCgggggc                                                                                    >  1:1519535/1‑92 (MQ=255)
       tGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCg                                                                                  >  2:1407396/1‑93 (MQ=255)
        gAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGa                                                                                 >  2:293524/1‑93 (MQ=255)
         aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAg                                                                                <  1:4343679/93‑1 (MQ=255)
         aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAg                                                                                <  1:2834861/93‑1 (MQ=255)
         aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAg                                                                                <  1:2688222/93‑1 (MQ=255)
          aTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAgg                                                                               >  1:1482460/1‑93 (MQ=255)
             cAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGgcgc                                                                            >  2:477772/1‑93 (MQ=255)
             cAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGgcgc                                                                            <  2:4649690/93‑1 (MQ=255)
               aTGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTg                                                                          >  2:208144/1‑93 (MQ=255)
                 gtgGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTa                                                                        <  1:4205732/93‑1 (MQ=255)
                 gtgGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTa                                                                        >  1:3178100/1‑93 (MQ=255)
                    ggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGc                                                                     >  1:3246229/1‑93 (MQ=255)
                       ggTGCTTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGa                                                                  <  1:2108999/93‑1 (MQ=255)
                       ggTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGa                                                                  <  1:4613474/93‑1 (MQ=255)
                        gTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGat                                                                 <  2:3217333/93‑1 (MQ=255)
                          gCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGatat                                                               >  1:3730058/1‑93 (MQ=255)
                          gCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGatat                                                               >  2:34435/1‑93 (MQ=255)
                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGTGCTGTATGCTGATATgg                                                             >  1:4639329/1‑93 (MQ=255)
                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             <  2:1123636/93‑1 (MQ=255)
                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             >  2:3895942/1‑93 (MQ=255)
                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             <  1:1217449/93‑1 (MQ=255)
                            aTTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATgg                                                             <  2:3871196/93‑1 (MQ=255)
                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            >  2:1666539/1‑93 (MQ=255)
                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            >  1:4044054/1‑93 (MQ=255)
                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            <  2:2852890/93‑1 (MQ=255)
                             tttCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATggg                                                            >  2:683349/1‑93 (MQ=255)
                               tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGc                                                          >  1:259587/1‑93 (MQ=255)
                               tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGc                                                          <  1:4023001/93‑1 (MQ=255)
                                cttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCa                                                         >  1:4390740/1‑93 (MQ=255)
                                 ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCAc                                                        >  2:2032093/1‑93 (MQ=255)
                                 ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCAc                                                        <  2:391985/93‑1 (MQ=255)
                                 ttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCAc                                                        >  2:1024755/1‑93 (MQ=255)
                                  tcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACt                                                       >  2:80260/1‑93 (MQ=255)
                                      tGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTgg                                                   <  1:4360505/93‑1 (MQ=255)
                                       gAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGt                                                  >  2:4343855/1‑93 (MQ=255)
                                       gAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGt                                                  <  1:4322521/93‑1 (MQ=255)
                                         aTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCGCTTTGGTaa                                                >  1:1123035/1‑93 (MQ=255)
                                         aTACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTaa                                                >  2:3609482/1‑93 (MQ=255)
                                           aCAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGt                                              >  1:2153348/1‑93 (MQ=255)
                                            cAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGtt                                             <  2:2689182/93‑1 (MQ=255)
                                            cAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGtt                                             <  2:3405117/93‑1 (MQ=255)
                                            cAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGtt                                             >  1:3422002/1‑93 (MQ=255)
                                             aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTc                                            >  2:1967586/1‑93 (MQ=255)
                                              aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTcc                                           >  1:4269382/1‑93 (MQ=255)
                                              aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTcc                                           <  1:136970/93‑1 (MQ=255)
                                              aaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGGGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGGACTTTGGTAAGTTcc                                           >  1:2746731/1‑93 (MQ=255)
                                               aaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTccc                                          <  1:4471152/93‑1 (MQ=255)
                                               aaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTccc                                          <  1:2073447/93‑1 (MQ=255)
                                                aCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCt                                         >  2:1163711/1‑93 (MQ=255)
                                                 cGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTa                                        <  2:4377009/93‑1 (MQ=255)
                                                  ggTTTCTTTTATTGCATTAGGGGCTGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       >  2:4518830/1‑93 (MQ=255)
                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       <  1:3485042/93‑1 (MQ=255)
                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       >  2:638000/1‑93 (MQ=255)
                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       <  1:1398378/93‑1 (MQ=255)
                                                  ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAt                                       >  2:3152588/1‑93 (MQ=255)
                                                   gTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAtt                                      >  1:1244721/1‑93 (MQ=255)
                                                   gTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAtt                                      >  2:737270/1‑93 (MQ=255)
                                                   gTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAtt                                      >  1:3752830/1‑93 (MQ=255)
                                                    tttCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTc                                     <  1:2876166/93‑1 (MQ=255)
                                                       cTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGcc                                  <  1:4550207/93‑1 (MQ=255)
                                                        ttttATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCt                                 >  2:2517136/1‑93 (MQ=255)
                                                          ttATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTgg                               <  2:4250728/93‑1 (MQ=255)
                                                           tATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGc                              >  1:4180011/1‑93 (MQ=255)
                                                           tATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGc                              <  1:3114812/93‑1 (MQ=255)
                                                           tATTGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGGCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGc                              >  1:1632744/1‑93 (MQ=255)
                                                             ttGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGt                            <  1:4154410/93‑1 (MQ=255)
                                                             ttGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGt                            >  2:3610773/1‑93 (MQ=255)
                                                              tGCATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTg                           >  1:800756/1‑93 (MQ=255)
                                                                cATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGt                         <  1:2181417/93‑1 (MQ=255)
                                                                cATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGt                         >  2:2956385/1‑93 (MQ=255)
                                                                cATTAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGt                         >  1:1639768/1‑93 (MQ=255)
                                                                  ttAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTc                       >  1:2498660/1‑93 (MQ=255)
                                                                  ttAGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTc                       >  1:1726256/1‑93 (MQ=255)
                                                                    aGGGGCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAc                     <  2:3562393/93‑1 (MQ=255)
                                                                      gggCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcg                   <  2:3008811/93‑1 (MQ=255)
                                                                      gggCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcg                   <  1:1328742/93‑1 (MQ=255)
                                                                       ggCAGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcgt                  <  2:4416710/93‑1 (MQ=255)
                                                                          aGTGGTGTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcgtcgt               >  1:4095974/1‑93 (MQ=255)
                                                                           gtggtgTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTa              >  2:3229478/1‑93 (MQ=255)
                                                                           gtggtgTTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTa              >  2:2485133/1‑93 (MQ=255)
                                                                              gtgtTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTa           <  1:2413544/93‑1 (MQ=255)
                                                                              gtgtTGTCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTa           >  2:3712886/1‑93 (MQ=255)
                                                                               tgttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAc          <  2:1814401/93‑1 (MQ=255)
                                                                                gttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAcc         >  1:618959/1‑93 (MQ=255)
                                                                                 ttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAcct        >  1:858159/1‑93 (MQ=255)
                                                                                 ttgtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAcct        >  1:1180263/1‑93 (MQ=255)
                                                                                   gtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttc      <  2:2606791/93‑1 (MQ=255)
                                                                                   gtCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttc      <  1:407403/93‑1 (MQ=255)
                                                                                    tCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcc     <  2:1855284/93‑1 (MQ=255)
                                                                                    tCGATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcc     >  1:2640640/1‑93 (MQ=255)
                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   <  2:2117014/93‑1 (MQ=255)
                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   >  1:739695/1‑93 (MQ=255)
                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   >  1:3887994/1‑93 (MQ=255)
                                                                                      gATTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTAccttcctt   >  1:1875269/1‑93 (MQ=255)
                                                                                       aTTGCGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCGTTg  >  2:1705904/1‑93 (MQ=255)
                                                                                          |                                                                                         
CATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTG  >  NC_000913/3929528‑3929707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑