BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,934,526	T→C	V208A (GTT→GCT)	rbsB →	D‑ribose periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,934,526	0	T	C	100.0%	853.4 / NA	265	V208A (GTT→GCT)	rbsB	D‑ribose periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (162/103); total (162/103)

Only 100 of 268 total aligned reads displayed.

AGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCG  >  NC_000913/3934436‑3934615

 gTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCa                                                                                        <  2:1090419/93‑1 (MQ=255)

 gTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCa                                                                                        >  2:2538758/1‑93 (MQ=255)

 gTTTAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCa                                                                                        <  1:1272833/93‑1 (MQ=255)

  tttAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAg                                                                                       >  1:4468814/1‑93 (MQ=255)

  tttAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAg                                                                                       <  1:3546623/93‑1 (MQ=255)

    tAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGc                                                                                     <  2:128107/93‑1 (MQ=255)

    tAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGc                                                                                     <  2:3616528/93‑1 (MQ=255)

    tAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGc                                                                                     >  2:4433727/1‑93 (MQ=255)

    tAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGc                                                                                     >  2:1064668/1‑93 (MQ=255)

    tAATGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGc                                                                                     >  1:1244936/1‑93 (MQ=255)

     aaTGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCt                                                                                    >  1:1007310/1‑93 (MQ=255)

     aaTGTTCTTGCCAGCCAGCCGGCAGACTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCt                                                                                    >  2:1732284/1‑93 (MQ=255)

      aTGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTg                                                                                   >  2:4106218/1‑93 (MQ=255)

      aTGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTg                                                                                   >  2:3447758/1‑93 (MQ=255)

      aTGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTg                                                                                   <  1:2043953/93‑1 (MQ=255)

       tGTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGt                                                                                  >  1:4617178/1‑93 (MQ=255)

        gTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTa                                                                                 >  1:3204447/1‑93 (MQ=255)

        gTTCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTa                                                                                 <  2:2378630/93‑1 (MQ=255)

         ttCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTAt                                                                                >  2:483195/1‑93 (MQ=255)

          tCTTGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTAtt                                                                               <  1:586858/93‑1 (MQ=255)

            ttGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTcg                                                                             <  2:3300224/93‑1 (MQ=255)

             tGCCAGCCAGCCGGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTcgc                                                                            <  2:424462/93‑1 (MQ=255)

              gccagccagccgGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTcgcg                                                                           >  2:2023076/1‑93 (MQ=255)

                cagccagccggcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCa                                                                         >  1:2937040/1‑93 (MQ=255)

                      gccggcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAatga                                                                   <  2:1212215/93‑1 (MQ=255)

                      gccggcAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAatga                                                                   <  2:2829933/93‑1 (MQ=255)

                        cggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAatgatg                                                                 >  1:823490/3‑93 (MQ=255)

                         ggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAtgatga                                                                <  2:1714925/92‑1 (MQ=255)

                         ggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAtgatga                                                                <  1:239541/92‑1 (MQ=255)

                         ggcagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAAtgatga                                                                >  1:2252677/2‑93 (MQ=255)

                           cagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGaaa                                                              <  2:3337509/93‑1 (MQ=255)

                           cagATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGaaa                                                              <  1:1072006/93‑1 (MQ=255)

                            agATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAAt                                                             >  1:730223/1‑93 (MQ=255)

                             gATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATg                                                            >  2:1875562/1‑93 (MQ=255)

                             gATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATg                                                            >  1:3885464/1‑93 (MQ=255)

                             gATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATg                                                            <  2:3848489/93‑1 (MQ=255)

                             gATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATg                                                            >  1:638050/1‑93 (MQ=255)

                              aTTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATgg                                                           <  1:376290/93‑1 (MQ=255)

                              aTTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATgg                                                           <  2:3575979/93‑1 (MQ=255)

                               ttttGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGgc                                                          >  1:3425948/1‑93 (MQ=255)

                                tttGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGgcg                                                         <  1:3269043/93‑1 (MQ=255)

                                  tGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCt                                                       >  1:2847670/1‑93 (MQ=255)

                                  tGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCt                                                       >  1:252829/1‑93 (MQ=255)

                                   gATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTg                                                      <  2:944875/93‑1 (MQ=255)

                                    aTCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTgg                                                     >  1:3351213/1‑93 (MQ=255)

                                     tCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTggg                                                    >  1:1489105/1‑93 (MQ=255)

                                     tCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTggg                                                    <  1:2719814/93‑1 (MQ=255)

                                       gCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcg                                                  >  1:2619792/1‑93 (MQ=255)

                                        cATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgc                                                 <  1:4367700/93‑1 (MQ=255)

                                         aTTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcg                                                <  2:1524396/93‑1 (MQ=255)

                                         aTTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcg                                                >  1:2749801/1‑93 (MQ=255)

                                          ttAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGgcgcgc                                               >  2:2354602/1‑93 (MQ=255)

                                           tAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCt                                              >  2:4662098/1‑93 (MQ=255)

                                           tAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCt                                              >  2:2188132/1‑93 (MQ=255)

                                            aaaGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGAGCTGGGCGCGCTg                                             >  2:44164/1‑93 (MQ=255)

                                             aaGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgc                                            >  1:1834343/1‑93 (MQ=255)

                                             aaGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgc                                            <  2:3797385/93‑1 (MQ=255)

                                             aaGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgc                                            >  2:4478668/1‑93 (MQ=255)

                                             aaGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgc                                            >  2:2367371/1‑93 (MQ=255)

                                              aGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcg                                           <  1:3882916/93‑1 (MQ=255)

                                                gTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgcg                                         >  1:1177898/1‑93 (MQ=255)

                                                 tttGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTgcgcgc                                        >  1:3057949/1‑93 (MQ=255)

                                                    gAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACt                                     >  2:2717747/1‑93 (MQ=255)

                                                      aCGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGc                                   <  1:4224550/93‑1 (MQ=255)

                                                       cGTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCa                                  >  2:160997/1‑93 (MQ=255)

                                                        gTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCaa                                 >  2:2100578/1‑93 (MQ=255)

                                                        gTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCaa                                 <  2:2220681/93‑1 (MQ=255)

                                                        gTAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCaa                                 >  2:2252052/1‑93 (MQ=255)

                                                         tAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCaaa                                <  2:3498771/93‑1 (MQ=255)

                                                         tAATGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGAGCTGGGCGCGCTGCGCGCACTGCaaa                                >  2:4324225/1‑93 (MQ=255)

                                                          aaTGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                               <  2:3406248/93‑1 (MQ=255)

                                                          aaTGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAAc                               <  2:4177512/93‑1 (MQ=255)

                                                           aTGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACt                              >  2:2587588/1‑93 (MQ=255)

                                                           aTGCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACt                              >  2:3247556/1‑93 (MQ=255)

                                                             gCAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGc                            >  2:1352314/1‑93 (MQ=255)

                                                              cAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGcc                           <  1:2337881/93‑1 (MQ=255)

                                                              cAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGcc                           <  1:1998377/93‑1 (MQ=255)

                                                              cAGAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGcc                           >  2:1944130/1‑93 (MQ=255)

                                                                gAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCgg                         <  1:270865/93‑1 (MQ=255)

                                                                gAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCgg                         >  2:2005527/1‑93 (MQ=255)

                                                                gAACCTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCgg                         >  1:91698/1‑93 (MQ=255)

                                                                 aaCCTGTTGACCGCTCATCCAGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGt                        >  2:2667667/1‑93 (MQ=255)

                                                                   ccTGTTGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTaa                      >  1:155261/1‑93 (MQ=255)

                                                                       ttGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCg                  <  1:67/93‑1 (MQ=255)

                                                                       ttGACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCg                  >  2:805380/1‑93 (MQ=255)

                                                                         gACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGa                >  2:62396/1‑93 (MQ=255)

                                                                         gACCGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGa                >  1:3642545/1‑93 (MQ=255)

                                                                           ccGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGAtg              >  1:4614896/1‑93 (MQ=255)

                                                                            cGCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGAtgt             <  1:1384810/93‑1 (MQ=255)

                                                                             gCTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGAtgtg            >  2:3910041/1‑93 (MQ=255)

                                                                              cTCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGa           >  2:4676465/1‑93 (MQ=255)

                                                                               tCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGAt          <  2:3204447/93‑1 (MQ=255)

                                                                               tCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGAt          <  2:2702254/93‑1 (MQ=255)

                                                                               tCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGAt          >  1:444184/1‑93 (MQ=255)

                                                                               tCATCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGAt          >  1:1582314/1‑93 (MQ=255)

                                                                                  tCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgt       <  2:739832/93‑1 (MQ=255)

                                                                                  tCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgt       <  1:3982014/93‑1 (MQ=255)

                                                                                  tCCGGATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgt       >  1:484493/1‑93 (MQ=255)

                                                                                      gATGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGgtcgtc   >  1:4308950/1‑93 (MQ=255)

                                                                                       aTGCTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGtcgtcg  >  1:1751855/1‑93 (MQ=255)

AGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGATCGCATTAAAGGTTTGAACGTAATGCAGAACCTGTTGACCGCTCATCCGGATGTTCAGGCTGTATTCGCGCAGAATGATGAAATGGCGCTGGGCGCGCTGCGCGCACTGCAAACTGCCGGTAAATCGGATGTGATGGTCGTCG  >  NC_000913/3934436‑3934615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`