BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	51,382	C→T	A75A (GCG→GCA)	apaG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	51,382	0	C	T	98.0%	500.1 / ‑3.2	146	A75A (GCG→GCA)	apaG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (77/66); total (79/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 153 total aligned reads displayed.

ATTTTCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAA  >  NC_000913/51292‑51471

aTTTTCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGgtgc                                                                                         <  2:4458996/93‑1 (MQ=255)

aTTTTCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGgtgc                                                                                         >  2:2548725/1‑93 (MQ=255)

 ttttCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGtgcg                                                                                        <  1:2637089/93‑1 (MQ=255)

  tttCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGa                                                                                       >  1:3194378/1‑93 (MQ=255)

    tCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATa                                                                                     >  2:345055/1‑93 (MQ=255)

    tCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATa                                                                                     >  2:2911911/1‑93 (MQ=255)

     taTCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTTGTGTACTGGTACTCTTCGCCAGGTGCGATaa                                                                                    <  1:2318447/92‑1 (MQ=255)

     cATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATaa                                                                                    <  2:148694/93‑1 (MQ=255)

      aTCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAg                                                                                   >  2:4537483/1‑93 (MQ=255)

      aTCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGCTGTACTGGTACTCTTCGCCAGGTGCGATAAg                                                                                   >  2:674361/1‑93 (MQ=255)

       tCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGt                                                                                  <  1:3733756/93‑1 (MQ=255)

       tCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGt                                                                                  >  2:3408399/1‑93 (MQ=255)

        cGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTg                                                                                 <  2:2350876/93‑1 (MQ=255)

         gATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTgg                                                                                >  2:1846875/1‑93 (MQ=255)

         gATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGGTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTgg                                                                                >  1:4284462/1‑93 (MQ=255)

          aTCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGc                                                                               >  2:2144125/1‑93 (MQ=255)

           tCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCt                                                                              >  2:3598770/1‑93 (MQ=255)

            cATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTg                                                                             <  1:1470474/93‑1 (MQ=255)

            cATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTg                                                                             <  1:3031604/93‑1 (MQ=255)

            cATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTg                                                                             <  1:3840234/93‑1 (MQ=255)

              ttttGTAGTGACCCTGCATGGTGCCCATCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGa                                                                           <  1:3171457/93‑1 (MQ=255)

              tttCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGa                                                                           >  1:2430193/1‑93 (MQ=255)

              tttCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGa                                                                           <  2:4568321/93‑1 (MQ=255)

               ttCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGAc                                                                          <  1:3105408/93‑1 (MQ=255)

                 cGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGc                                                                        >  2:2338409/1‑93 (MQ=255)

                  gTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGcc                                                                       >  1:3848752/1‑93 (MQ=255)

                  gTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGcc                                                                       >  2:572420/1‑93 (MQ=255)

                  gTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGcc                                                                       <  2:4375331/93‑1 (MQ=255)

                  gTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGcc                                                                       >  1:1223545/1‑93 (MQ=255)

                   tAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCa                                                                      >  1:778760/1‑93 (MQ=255)

                   tAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCa                                                                      >  2:2070290/1‑93 (MQ=255)

                   tAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCa                                                                      >  1:4059759/1‑93 (MQ=255)

                      tGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAAcc                                                                   >  2:4104258/1‑93 (MQ=255)

                      tGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAAcc                                                                   <  1:1278997/93‑1 (MQ=255)

                       gACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCa                                                                  >  2:4400434/1‑93 (MQ=255)

                       gACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCa                                                                  <  1:1127872/93‑1 (MQ=255)

                       gACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCa                                                                  <  2:1163107/93‑1 (MQ=255)

                        aCCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCAc                                                                 >  2:4064845/1‑93 (MQ=255)

                        aCCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCAc                                                                 >  2:2855640/1‑93 (MQ=255)

                         cccTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACt                                                                <  2:313031/93‑1 (MQ=255)

                          ccTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTc                                                               <  1:1535828/93‑1 (MQ=255)

                          ccTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTc                                                               >  1:4479993/1‑93 (MQ=255)

                            tGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCt                                                             <  1:1613819/93‑1 (MQ=255)

                            tGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCt                                                             >  1:2039600/1‑93 (MQ=255)

                            tGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCt                                                             <  2:2817498/93‑1 (MQ=255)

                            tGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCt                                                             >  1:898509/1‑93 (MQ=255)

                              cATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTc                                                           >  1:41355/1‑93 (MQ=255)

                               aTGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCg                                                          >  1:1488430/1‑93 (MQ=255)

                                tGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGc                                                         >  2:4323233/1‑93 (MQ=255)

                                tGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGc                                                         >  1:1698890/1‑93 (MQ=255)

                                    gCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTg                                                     >  1:2425445/1‑93 (MQ=255)

                                     cccAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTgg                                                    >  2:2317921/1‑93 (MQ=255)

                                      ccAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGa                                                   >  2:177295/1‑93 (MQ=255)

                                      ccAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGa                                                   >  1:1112210/1‑93 (MQ=255)

                                      ccAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGa                                                   >  1:2865616/1‑93 (MQ=255)

                                       cAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGAc                                                  >  1:242540/1‑93 (MQ=255)

                                        aGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACt                                                 <  1:2453464/93‑1 (MQ=255)

                                        aGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACt                                                 >  2:2589246/1‑93 (MQ=255)

                                          gggggTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGGGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTc                                               <  1:3298511/92‑1 (MQ=255)

                                          cGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTc                                               >  1:2883256/1‑93 (MQ=255)

                                            gggTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCgg                                             >  2:2579072/1‑93 (MQ=255)

                                               tttCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCAGttt                                          >  1:969018/1‑93 (MQ=255)

                                                ttCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTc                                         >  1:4199078/1‑93 (MQ=255)

                                                  cAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCAc                                       >  1:3107464/1‑93 (MQ=255)

                                                   aaTGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACg                                      <  2:3113441/93‑1 (MQ=255)

                                                     tGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgc                                    >  2:4513693/1‑93 (MQ=255)

                                                        ttGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccat                                 >  1:3083956/1‑93 (MQ=255)

                                                        ttGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccat                                 >  2:1168905/1‑93 (MQ=255)

                                                        ttGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccat                                 <  1:33810/93‑1 (MQ=255)

                                                         tGCACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccatt                                >  1:2771761/1‑93 (MQ=255)

                                                           cACCGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccattgc                              <  2:1465697/93‑1 (MQ=255)

                                                             ccGCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccattgcca                            >  1:1266951/1‑93 (MQ=255)

                                                               gCTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccattgccatt                          >  1:3553140/1‑93 (MQ=255)

                                                                cTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGccattgccattg                         <  2:3848960/93‑1 (MQ=255)

                                                                cTGGTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGccattgccattg                         <  2:768247/93‑1 (MQ=255)

                                                                 tGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTgg                        <  2:2066074/93‑1 (MQ=255)

                                                                  ggTGTACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGt                       <  2:1756196/93‑1 (MQ=255)

                                                                   gtgtACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTg                      >  1:2597908/1‑93 (MQ=255)

                                                                    tgtACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGa                     <  2:2427344/93‑1 (MQ=255)

                                                                      tACTGGTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATc                   <  2:450329/93‑1 (MQ=255)

                                                                         tGGTACTCTTCGCCACGTGCGAGAAGTGGGTGGTCGACAACCACTATTTCGAACTGGGCGTAGGTTTGAAGGACATTGCCATTGGTGATCAgt                >  2:1641151/1‑92 (MQ=255)

                                                                           gTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCa              >  2:3944387/1‑93 (MQ=255)

                                                                           gTACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCa              >  1:492796/1‑93 (MQ=255)

                                                                            tACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAg             <  1:1001740/93‑1 (MQ=255)

                                                                            tACTCTTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGACATTGCCATTGGTGATCAGCCAg             >  1:3746911/1‑93 (MQ=255)

                                                                              ctctTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTa           <  2:1785969/93‑1 (MQ=255)

                                                                              ctctTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTa           >  1:3994760/1‑93 (MQ=255)

                                                                               tctTCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTaa          >  1:2545015/1‑93 (MQ=255)

                                                                                 ttCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACg        >  2:1224880/1‑93 (MQ=255)

                                                                                 ttCGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGGGATCAGCCAGTAACg        >  2:2116035/1‑93 (MQ=255)

                                                                                 ttCGCCAGGTGCGATAAGTGGCTGGACGCAAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACg        <  2:1534757/93‑1 (MQ=255)

                                                                                  tCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGc       >  1:2748369/1‑93 (MQ=255)

                                                                                   cGCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGcc      >  2:410972/1‑93 (MQ=255)

                                                                                    gCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGccc     >  1:1843197/1‑93 (MQ=255)

                                                                                    gCCAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGccc     <  2:886934/93‑1 (MQ=255)

                                                                                     ccAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGcccc    <  2:3585629/93‑1 (MQ=255)

                                                                                      cAGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCca   >  2:3266051/1‑93 (MQ=255)

                                                                                       aGGTGCGATAAGTGGCTGGACGCCAACCCCTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCcaa  <  2:967957/93‑1 (MQ=255)

                                                                                       aGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCcaa  <  2:321661/93‑1 (MQ=255)

                                                                                       aGGTGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCcaa  <  2:3004806/93‑1 (MQ=255)

ATTTTCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAA  >  NC_000913/51292‑51471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`