BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,961,966	A→G	intergenic (‑122/+14)	gppA ← / ← rhlB	guanosine pentaphosphatase/putative ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,961,966	0	A	G	100.0%	919.7 / NA	285	intergenic (‑122/+14)	gppA/rhlB	guanosine pentaphosphatase/putative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (151/134); total (151/134)

Only 100 of 290 total aligned reads displayed.

GCTGCATTCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTATGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAGGCGC  >  NC_000913/3961876‑3962055

 cTGCATTCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGa                                                                                        <  2:4621986/93‑1 (MQ=255)

  tGCATTCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGAc                                                                                       >  1:2495013/1‑93 (MQ=255)

  tGCATTCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGAc                                                                                       >  2:450093/1‑93 (MQ=255)

     aTTCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACAtt                                                                                    >  2:1079650/1‑93 (MQ=255)

      ttCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACAttt                                                                                   <  2:3777028/93‑1 (MQ=255)

       tCTTTCGCCTGAATCACTTACGACACTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACAtttt                                                                                  <  2:4184395/93‑1 (MQ=37)

       tCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACAtttt                                                                                  <  2:3689073/93‑1 (MQ=255)

         tttCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACAtttttt                                                                                >  2:587095/1‑93 (MQ=255)

         tttCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACAtttttt                                                                                >  1:1306993/1‑93 (MQ=255)

          ttCACCTCAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTttta                                                                               <  1:1518682/93‑1 (MQ=255)

           ccACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTtttat                                                                              >  1:1965227/2‑93 (MQ=255)

             aCCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTtttattt                                                                            >  2:4002319/1‑93 (MQ=255)

             aCCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTtttattt                                                                            <  1:2679439/93‑1 (MQ=255)

               cTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTaa                                                                          <  1:3116111/93‑1 (MQ=255)

               cTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTaa                                                                          <  2:4526917/93‑1 (MQ=255)

               cTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGCGACATTTTTTATTTaa                                                                          >  1:2131082/1‑93 (MQ=255)

                tGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAAc                                                                         >  2:2378262/1‑93 (MQ=255)

                tGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTACCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAAc                                                                         >  1:3295034/1‑93 (MQ=255)

                 gAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAAcc                                                                        <  1:2395418/93‑1 (MQ=255)

                 gAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAAcc                                                                        <  1:287254/93‑1 (MQ=255)

                  aaTCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCt                                                                       <  1:403/93‑1 (MQ=255)

                    tCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGa                                                                     <  1:3717789/93‑1 (MQ=255)

                      aCTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAac                                                                   <  2:552772/93‑1 (MQ=255)

                        ttACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAacga                                                                 <  1:4541327/93‑1 (MQ=255)

                        ttACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAacga                                                                 >  1:1618556/1‑93 (MQ=255)

                         tactacAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAacgac                                                                >  2:223638/1‑93 (MQ=255)

                         tactacAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAacgac                                                                <  1:1136091/93‑1 (MQ=255)

                           ctacAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAacgacga                                                              <  1:3327273/93‑1 (MQ=255)

                             acAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAacgacgacg                                                            >  1:3520003/1‑93 (MQ=255)

                             acAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAacgacgacg                                                            >  1:2786970/1‑93 (MQ=255)

                              cAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAAcgacgacga                                                           <  2:964605/93‑1 (MQ=255)

                              cAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAAcgacgacga                                                           <  2:2949303/93‑1 (MQ=255)

                                gTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGAtt                                                         <  2:3482967/93‑1 (MQ=255)

                                gTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGAtt                                                         <  1:2896021/93‑1 (MQ=255)

                                 tAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTa                                                        <  1:1796070/93‑1 (MQ=255)

                                 tAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTa                                                        <  1:3877614/93‑1 (MQ=255)

                                  aaGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTAc                                                       <  1:3215564/93‑1 (MQ=255)

                                  aaGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTAc                                                       <  2:4053770/93‑1 (MQ=255)

                                   aGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTAcg                                                      >  1:499617/1‑93 (MQ=255)

                                    gCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTAcgc                                                     >  2:854432/1‑93 (MQ=255)

                                     cTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTAcgcg                                                    >  2:2451225/1‑93 (MQ=255)

                                     cTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTAcgcg                                                    >  2:2305929/1‑93 (MQ=255)

                                      tCACCGGGATTCATTCTCTTGTCATCTTGATACGGTTTCAATGATTTTGAGTGTGCTATTTTTTATTTAACCTGAACGACGACGATTACGCgg                                                   >  2:1523512/1‑93 (MQ=255)

                                       cATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGa                                                  <  2:3274872/93‑1 (MQ=255)

                                       cATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGa                                                  <  2:274415/93‑1 (MQ=255)

                                        aTCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAg                                                 <  2:3086244/93‑1 (MQ=255)

                                        aTCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAg                                                 >  2:715161/1‑93 (MQ=255)

                                          cGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAgcg                                               >  1:1002800/1‑93 (MQ=255)

                                            ggATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGcc                                             >  1:1585193/1‑93 (MQ=255)

                                            ggATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGcc                                             >  2:1560103/1‑93 (MQ=255)

                                              attcattcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAg                                           >  1:2247222/1‑93 (MQ=255)

                                              attcattcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAg                                           >  1:1356115/1‑93 (MQ=255)

                                              attcattcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAg                                           >  2:2020507/1‑93 (MQ=255)

                                               ttcattcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGt                                          >  2:4222389/1‑93 (MQ=255)

                                                tcattcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTa                                         <  1:929676/93‑1 (MQ=255)

                                                 cattcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTac                                        >  1:2701597/1‑93 (MQ=255)

                                                  attcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTACCCTGAACGACGACGATTACGCGGAGCGCCAGTacg                                       <  2:2470765/93‑1 (MQ=255)

                                                  attcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTacg                                       >  2:1245693/1‑93 (MQ=255)

                                                   ttcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTacgc                                      >  1:429697/1‑92 (MQ=255)

                                                   ttcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTacga                                      <  2:3067792/93‑1 (MQ=255)

                                                   ttcTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTacga                                      >  1:3405847/1‑93 (MQ=255)

                                                    tctcTTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTacgac                                     <  2:3723641/93‑1 (MQ=255)

                                                     ctctTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTacgacg                                    >  1:1937248/1‑93 (MQ=255)

                                                     ctctTGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTacgacg                                    >  1:1175765/1‑93 (MQ=255)

                                                        ttGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCgg                                 >  2:3895613/1‑93 (MQ=255)

                                                         tGCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGa                                <  1:4550859/93‑1 (MQ=255)

                                                          gCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGAc                               >  2:3649545/1‑93 (MQ=255)

                                                          gCCATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGAc                               >  2:3457608/1‑93 (MQ=255)

                                                           ccATCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGAcc                              >  1:3661691/1‑93 (MQ=255)

                                                            cATCTTGATACAGTTTGAATTATTTTGAGTGTGACATTTGTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCa                             <  1:707749/93‑1 (MQ=255)

                                                             aTCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCAt                            >  2:4261473/1‑93 (MQ=255)

                                                             aTCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCAt                            >  1:4310307/1‑93 (MQ=255)

                                                             aTCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCAt                            >  1:257755/1‑93 (MQ=255)

                                                              tCTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCAtt                           >  1:831760/1‑93 (MQ=255)

                                                               cTTGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTg                          >  2:1441399/1‑93 (MQ=255)

                                                                ttGATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGc                         >  1:2973034/1‑93 (MQ=255)

                                                                  gATACAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCt                       >  2:251759/1‑93 (MQ=255)

                                                                      cAGTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTgc                   <  2:2213280/93‑1 (MQ=255)

                                                                        gTTTGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTgcgg                 >  1:1983398/1‑92 (MQ=255)

                                                                          ttGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCgg               <  1:1831707/93‑1 (MQ=255)

                                                                           tGAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCggg              >  2:3208963/1‑93 (MQ=255)

                                                                            taaTGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGgc             <  1:3828358/92‑1 (MQ=255)

                                                                            gAATGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGgc             >  2:1971042/1‑93 (MQ=255)

                                                                              aTGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGgcgc           >  1:4079497/1‑93 (MQ=255)

                                                                              aTGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGgcgc           >  2:3941873/1‑93 (MQ=255)

                                                                               tGATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGcgcg          <  2:506620/93‑1 (MQ=255)

                                                                                gATTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGt         >  2:778651/1‑93 (MQ=255)

                                                                                 aTTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTg        >  2:3870591/1‑93 (MQ=255)

                                                                                 aTTTTGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTg        <  1:4351403/93‑1 (MQ=255)

                                                                                  ttttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGa       >  1:3139706/1‑93 (MQ=255)

                                                                                  ttttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGa       <  1:2558793/93‑1 (MQ=255)

                                                                                  ttttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGa       <  2:1872978/93‑1 (MQ=255)

                                                                                  ttttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGa       >  2:1655559/1‑93 (MQ=255)

                                                                                   tttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAg      <  2:1003650/93‑1 (MQ=255)

                                                                                    ttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAgg     >  2:4464878/1‑93 (MQ=255)

                                                                                    ttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAgg     <  2:2106629/93‑1 (MQ=255)

                                                                                    ttGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAgg     <  1:419824/93‑1 (MQ=255)

                                                                                     tGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAGgc    <  1:1489688/93‑1 (MQ=255)

                                                                                     tGAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAGgc    >  2:1132740/1‑93 (MQ=255)

                                                                                      gAGTGTGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAGgcg   >  2:4309189/1‑93 (MQ=255)

GCTGCATTCTTTCACCTGAATCACTTACTACAGTAAGCTCATCGGGATTCATTCTCTTGCCATCTTGATACAGTTTGAATGATTTTGAGTATGACATTTTTTATTTAACCTGAACGACGACGATTACGCGGAGCGCCAGTACGACGCGGACCATTGCCTGTGCGCGGGCGCGTGAGGCGC  >  NC_000913/3961876‑3962055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`