BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,962,662	A→G	M195T (ATG→ACG)	rhlB ←	putative ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,962,662	0	A	G	100.0%	603.3 / NA	193	M195T (ATG→ACG)	rhlB	putative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (82/111); total (82/111)

Only 100 of 205 total aligned reads displayed.

TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751

tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGGAAGCGTGGCGGAGAACAGCGTg                                                                                         >  2:3441763/1‑93 (MQ=255)

 gTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCgtgt                                                                                        <  2:3895287/93‑1 (MQ=255)

  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGCGAACAGCGTGtt                                                                                       >  2:2386016/1‑93 (MQ=255)

  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGGACAGCGTGtt                                                                                       >  2:1234059/1‑93 (MQ=255)

  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGCACAGCGTGtt                                                                                       >  1:2597957/1‑93 (MQ=255)

  ttCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGtt                                                                                       <  2:4613373/93‑1 (MQ=255)

   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      >  1:2984966/1‑93 (MQ=255)

   tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTg                                                                                      >  2:3752363/1‑93 (MQ=255)

    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  1:516960/93‑1 (MQ=255)

    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  1:2689143/93‑1 (MQ=255)

    ccGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGa                                                                                     <  2:1942562/93‑1 (MQ=255)

      ggTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAgg                                                                                   <  2:105098/93‑1 (MQ=255)

        ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                                 >  1:607591/1‑93 (MQ=255)

        ttCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcg                                                                                 >  2:764090/1‑93 (MQ=255)

         tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGgcgc                                                                                <  2:2589586/93‑1 (MQ=255)

          ccACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCt                                                                               >  2:3986511/1‑93 (MQ=255)

           cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTg                                                                              <  2:4125941/93‑1 (MQ=255)

           cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTg                                                                              <  2:2054324/93‑1 (MQ=255)

           cACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGGGGTGAGGCGCTg                                                                              >  2:4279768/1‑93 (MQ=255)

             cTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGt                                                                            <  1:3434133/93‑1 (MQ=255)

              ttCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGtt                                                                           <  2:58043/93‑1 (MQ=255)

                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  2:1614015/93‑1 (MQ=255)

                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       <  2:2527405/93‑1 (MQ=255)

                  atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCa                                                                       >  2:4181042/1‑93 (MQ=255)

                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      <  1:4430881/93‑1 (MQ=255)

                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  2:3520595/1‑93 (MQ=255)

                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  2:4030630/1‑93 (MQ=255)

                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAg                                                                      >  1:3020379/1‑93 (MQ=255)

                   tatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGAGCTGGTTTGCAg                                                                      >  1:3207514/1‑93 (MQ=255)

                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     >  1:527710/1‑93 (MQ=255)

                    atatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAgg                                                                     <  1:279178/93‑1 (MQ=255)

                     tatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggc                                                                    >  1:4239708/1‑93 (MQ=255)

                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   <  1:3354614/93‑1 (MQ=255)

                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   <  1:1707740/93‑1 (MQ=255)

                      atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGCGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcg                                                                   >  1:1834462/1‑93 (MQ=255)

                       ttCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTCCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAggcgg                                                                  <  2:443509/93‑1 (MQ=255)

                         cGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCa                                                                <  1:1322325/93‑1 (MQ=255)

                          ggCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCAt                                                               >  1:3722607/1‑93 (MQ=255)

                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              <  1:2899252/93‑1 (MQ=255)

                           gCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATa                                                              <  1:4290834/93‑1 (MQ=255)

                            cATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAc                                                             <  1:1159833/93‑1 (MQ=255)

                            cATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAc                                                             <  1:2403903/93‑1 (MQ=255)

                             aTTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcg                                                            >  1:2854023/1‑93 (MQ=255)

                              ttGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGGAGGAGGCATAcag                                                           >  2:147145/1‑91 (MQ=255)

                               tGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggc                                                          <  1:3045437/93‑1 (MQ=255)

                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         <  1:448405/93‑1 (MQ=255)

                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         >  2:825044/1‑93 (MQ=255)

                                gTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                         >  2:1763305/1‑93 (MQ=255)

                                 ttCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcgg                                                        <  1:3502759/93‑1 (MQ=255)

                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGGAc                                                     >  2:954546/1‑93 (MQ=255)

                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAc                                                     <  2:2850391/93‑1 (MQ=255)

                                    aTCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAc                                                     >  1:3418825/1‑93 (MQ=255)

                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGCACAGCGCGTTGAGTCGCTGGTTTGCAGGCGGCATTcggcgggagc                                                    >  2:4105132/1‑89 (MQ=255)

                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    >  2:2913684/1‑93 (MQ=255)

                                     tCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                    >  1:403123/1‑93 (MQ=255)

                                      cTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcag                                                   <  1:1998060/93‑1 (MQ=255)

                                         cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcca                                                <  1:1192460/93‑1 (MQ=255)

                                         cTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcca                                                <  1:2018835/93‑1 (MQ=255)

                                          tCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccag                                               <  2:3719375/93‑1 (MQ=255)

                                            gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGGACAGCCAGCg                                             >  2:1826297/1‑93 (MQ=255)

                                            gAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCg                                             <  1:3621999/93‑1 (MQ=255)

                                             aaCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCgg                                            <  1:2824994/93‑1 (MQ=255)

                                              aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                           >  1:3104778/1‑93 (MQ=255)

                                              aCGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                           <  1:436937/93‑1 (MQ=255)

                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         <  1:3925019/93‑1 (MQ=255)

                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         <  1:1396546/93‑1 (MQ=255)

                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         <  2:202359/93‑1 (MQ=255)

                                                gCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGCGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGata                                         >  1:1733556/1‑93 (MQ=255)

                                                 ccAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGatat                                        >  1:461019/1‑93 (MQ=255)

                                                  cAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATc                                       <  2:88688/93‑1 (MQ=255)

                                                   aGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCt                                      <  1:711645/93‑1 (MQ=255)

                                                     ttCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCttt                                    <  1:1903558/93‑1 (MQ=255)

                                                      tCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTta                                   <  1:957368/93‑1 (MQ=255)

                                                       cACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaa                                  <  1:1457045/93‑1 (MQ=255)

                                                       cACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaa                                  <  2:159280/93‑1 (MQ=255)

                                                        aCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaat                                 >  2:4340000/1‑93 (MQ=255)

                                                         cGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaata                                <  1:2232054/93‑1 (MQ=255)

                                                         cGTACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaata                                <  1:882530/93‑1 (MQ=255)

                                                           tACCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaa                              <  2:1554433/93‑1 (MQ=255)

                                                            aCCCGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAg                             >  2:3947318/1‑93 (MQ=255)

                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaaaac                           >  2:2754997/1‑90 (MQ=255)

                                                              ccGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                           <  1:680420/93‑1 (MQ=255)

                                                               cGGTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGccc                          <  1:1797067/93‑1 (MQ=255)

                                                                ggTACGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                         <  2:2999711/93‑1 (MQ=255)

                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  1:3468554/93‑1 (MQ=255)

                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  2:1896992/93‑1 (MQ=255)

                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  1:3509528/93‑1 (MQ=255)

                                                                   aCGAAAGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGAt                      <  1:4674/93‑1 (MQ=255)

                                                                      aaaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGt                   >  1:214156/1‑93 (MQ=255)

                                                                      aaaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGt                   >  1:2273497/1‑93 (MQ=255)

                                                                      aaaGCGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGt                   >  1:759355/1‑93 (MQ=255)

                                                                          cGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt               <  2:2456504/93‑1 (MQ=255)

                                                                          cGTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt               <  2:1420616/93‑1 (MQ=255)

                                                                           gTGGCGGAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATg              <  1:999436/93‑1 (MQ=255)

                                                                                ggAGAACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATc         <  2:1644823/93‑1 (MQ=255)

                                                                                 gagaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCg        >  1:2214421/1‑93 (MQ=255)

                                                                                   gaACAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGc      >  2:331702/1‑93 (MQ=255)

                                                                                    aaCAGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt     >  1:3810936/1‑93 (MQ=255)

                                                                                     aCAGCGGGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCtt    <  1:3074323/93‑1 (MQ=255)

                                                                                       aGCGTGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg  >  1:2693651/1‑93 (MQ=255)

TGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCG  >  NC_000913/3962572‑3962751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`