BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	442,390	C→T	G159D (GGT→GAT)	thiJ ←	4‑methyl‑5(beta‑hydroxyethyl)‑thiazole monophosphate synthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	442,390	0	C	T	100.0%	584.5 / NA	162	G159D (GGT→GAT)	thiJ	4‑methyl‑5(beta‑hydroxyethyl)‑thiazole monophosphate synthesis
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (94/68); total (94/68)

Only 100 of 168 total aligned reads displayed.

GCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGC  >  NC_000913/442300‑442479

gccgccATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAtcc                                                                                         <  1:534378/93‑1 (MQ=255)

 ccgccATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCg                                                                                        <  2:3007179/93‑1 (MQ=255)

 ccgccATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCg                                                                                        <  2:662985/93‑1 (MQ=255)

  cgccATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCgg                                                                                       <  2:1950698/93‑1 (MQ=255)

   gccATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGc                                                                                      >  2:2418464/1‑93 (MQ=255)

     cATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGccc                                                                                    >  1:4315999/1‑93 (MQ=255)

     cATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGccc                                                                                    >  1:1504321/1‑93 (MQ=255)

     cATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGccc                                                                                    >  1:2219185/1‑93 (MQ=255)

     cATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGccc                                                                                    >  2:1422234/1‑93 (MQ=255)

      aTCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGcccc                                                                                   <  1:2974580/93‑1 (MQ=255)

      aTCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGcccc                                                                                   <  1:2314909/93‑1 (MQ=255)

      aTCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGcccc                                                                                   <  1:1018676/93‑1 (MQ=255)

       tCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCct                                                                                  >  1:2080997/1‑93 (MQ=255)

       tCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCct                                                                                  >  2:1571302/1‑93 (MQ=255)

       tCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCct                                                                                  >  2:3300527/1‑93 (MQ=255)

        cACCAGGTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTGTCAGACCAAAGTCGATAGCTGTATCCGGCCCctg                                                                                 <  1:1449891/93‑1 (MQ=255)

         aCCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCctgg                                                                                >  2:1511896/1‑93 (MQ=255)

           cAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCctggcg                                                                              >  2:272642/1‑92 (MQ=255)

            aGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCctggctg                                                                             >  2:1107818/1‑93 (MQ=255)

            aGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCctggctg                                                                             <  1:4615963/93‑1 (MQ=255)

             gTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAGCAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCctggctgg                                                                            >  1:769164/1‑93 (MQ=255)

             gTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCctggctgg                                                                            <  2:4637644/93‑1 (MQ=255)

             gTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCctggctgg                                                                            >  1:2602911/1‑93 (MQ=255)

              ttGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGt                                                                           >  1:3391231/1‑93 (MQ=255)

                gtgATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCa                                                                         <  2:2960457/93‑1 (MQ=255)

                   aTGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCa                                                                      >  2:117812/1‑93 (MQ=255)

                   aTGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCa                                                                      >  1:623888/1‑93 (MQ=255)

                    tGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCaa                                                                     <  2:3237278/93‑1 (MQ=255)

                    tGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCaa                                                                     <  1:1268008/93‑1 (MQ=255)

                    tGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCaa                                                                     <  1:2021448/93‑1 (MQ=255)

                      ccACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAAtt                                                                   <  1:1842164/93‑1 (MQ=255)

                       cACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAAttt                                                                  >  2:431620/1‑93 (MQ=255)

                        aCTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAAtttt                                                                 >  1:384091/1‑93 (MQ=255)

                         cTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTa                                                                >  2:4431217/1‑93 (MQ=255)

                          ttCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTAc                                                               >  1:1070336/1‑93 (MQ=255)

                          ttCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTAc                                                               >  1:855231/1‑93 (MQ=255)

                            cATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTAccc                                                             >  2:2341396/1‑93 (MQ=255)

                             aTGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCg                                                            >  1:1922190/1‑93 (MQ=255)

                              tGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCgg                                                           >  1:3134595/1‑92 (MQ=255)

                              tGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGt                                                           >  2:2933881/1‑93 (MQ=255)

                                  cTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTCCCCGTGCAt                                                       <  1:4682341/93‑1 (MQ=255)

                                  cTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCAt                                                       >  2:2884976/1‑93 (MQ=255)

                                  cTTTTTCACGCCCAACCAACAGGCCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGcgt                                                       >  2:2605750/1‑91 (MQ=255)

                                   tttttCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATc                                                      <  2:2737304/93‑1 (MQ=255)

                                    ttttCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATcc                                                     >  2:849763/1‑93 (MQ=255)

                                     tttCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATccc                                                    >  2:1665732/1‑93 (MQ=255)

                                      ttCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCa                                                   <  2:2298354/93‑1 (MQ=255)

                                        cACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAga                                                 <  2:2208949/93‑1 (MQ=255)

                                         aCGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAgac                                                <  2:2453737/93‑1 (MQ=255)

                                            cccAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAgacgac                                             <  1:3386546/93‑1 (MQ=255)

                                             ccaaccaaCAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGacgacg                                            >  2:3514736/1‑93 (MQ=255)

                                               aaccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGAcgcg                                          <  1:84766/93‑1 (MQ=255)

                                               aaccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGAcgcg                                          >  2:4267875/1‑93 (MQ=255)

                                               aaccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGAcgcg                                          >  2:4544786/1‑93 (MQ=255)

                                                accaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACgcgc                                         <  1:12343/93‑1 (MQ=255)

                                                 ccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt                                        <  1:3647582/93‑1 (MQ=255)

                                                 ccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAACTTTACCCGTGCATCCCAGACGACGCGCt                                        <  1:1741483/93‑1 (MQ=255)

                                                  caacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCtt                                       <  2:3737756/93‑1 (MQ=255)

                                                   aacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTg                                      <  1:3414642/93‑1 (MQ=255)

                                                   aacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTg                                      >  1:3008026/1‑93 (MQ=255)

                                                     cAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTc                                    <  1:3833177/93‑1 (MQ=255)

                                                      aGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCTGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTcc                                   <  1:408697/93‑1 (MQ=255)

                                                       ggTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCa                                  >  2:545627/1‑93 (MQ=255)

                                                        gTCGATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAg                                 >  2:383454/1‑93 (MQ=255)

                                                           gATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCa                              <  1:3660357/93‑1 (MQ=255)

                                                           gATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCa                              >  1:2050553/1‑93 (MQ=255)

                                                           gATAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCa                              >  2:13479/1‑93 (MQ=255)

                                                             tAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAtt                            <  1:448699/93‑1 (MQ=255)

                                                             tAATTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAtt                            >  2:236256/1‑93 (MQ=255)

                                                               aTTTTCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGt                          <  2:1038698/93‑1 (MQ=255)

                                                                ttttCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGtt                         >  2:1710256/1‑93 (MQ=255)

                                                                ttttCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGtt                         >  2:1533279/1‑93 (MQ=255)

                                                                ttttCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGtt                         <  2:1353171/93‑1 (MQ=255)

                                                                ttttCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGtt                         >  1:557194/1‑93 (MQ=255)

                                                                 tttCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTc                        <  2:4302996/93‑1 (MQ=255)

                                                                 tttCAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTc                        <  1:1104076/93‑1 (MQ=255)

                                                                    cAGACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTcggc                     >  2:2486274/1‑93 (MQ=255)

                                                                     aGACCAAAGTCGATAGCTGTATCCGGCTCCTGGCTGGTCAGCCATTTTACCCGTGCATCCCAGACGACGCGCATGTCCAGCCATTGTTcggcg                    >  2:2059613/1‑93 (MQ=255)

                                                                      gACCAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTcggcgg                   <  2:1177450/93‑1 (MQ=255)

                                                                        ccAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATGCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGa                 >  2:4151013/1‑93 (MQ=255)

                                                                        ccAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGa                 >  2:872932/1‑93 (MQ=255)

                                                                        ccAAAGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGa                 >  1:3746662/1‑93 (MQ=255)

                                                                          aaaGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAAt               >  1:209275/1‑93 (MQ=255)

                                                                          aaaGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAAt               <  2:631495/93‑1 (MQ=255)

                                                                          aaaGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAAt               >  1:3822923/1‑93 (MQ=255)

                                                                           aaGTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAAtt              <  1:671586/93‑1 (MQ=255)

                                                                             gTCGATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTAGGCGGGGAtttt            >  2:3866422/1‑93 (MQ=255)

                                                                              tCGATAGCTGTATCCTGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGCATTTTg           >  1:2480389/1‑93 (MQ=255)

                                                                                gATAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTc         >  2:332879/1‑93 (MQ=255)

                                                                                 aTAGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCt        >  2:119696/1‑93 (MQ=255)

                                                                                 aTAGCTGCATCCGTCCCCTGGCGGGTCAGCACTTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTcc        <  1:4201962/93‑2 (MQ=255)

                                                                                   aGCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCttt      >  1:1492942/1‑93 (MQ=255)

                                                                                    gCTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTc     <  1:4540187/93‑1 (MQ=255)

                                                                                     cTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCa    <  1:321455/93‑1 (MQ=255)

                                                                                     cTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCa    <  2:3722782/93‑1 (MQ=255)

                                                                                     cTGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCa    >  2:3453936/1‑93 (MQ=255)

                                                                                      tGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAg   >  1:311632/1‑93 (MQ=255)

                                                                                      tGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAg   >  2:3676068/1‑93 (MQ=255)

                                                                                      tGTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAg   >  2:3509588/1‑93 (MQ=255)

                                                                                       gTATCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCCGACGACGCGCTTGTACAGCCATTGTTCGGCGGGAATTTTGTCTTTCAgg  >  1:4011537/1‑92 (MQ=255)

GCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGC  >  NC_000913/442300‑442479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`