BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	442,916	A→G	V275A (GTA→GCA)	apbA ←	ApbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	442,916	0	A	G	99.4%	547.1 / ‑5.2	178	V275A (GTA→GCA)	apbA	ApbA
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (94/83); total (94/84)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 191 total aligned reads displayed.

CACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAAC  >  NC_000913/442826‑443005

cACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCg                                                                                         >  1:4158328/1‑93 (MQ=255)

cACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCg                                                                                         <  1:823370/93‑1 (MQ=255)

 aCTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCgg                                                                                        >  1:1983817/1‑93 (MQ=255)

  cTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTTCGGTGCGGc                                                                                       >  2:4047522/1‑93 (MQ=255)

   tACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCa                                                                                      >  1:992838/1‑93 (MQ=255)

    aCCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCaa                                                                                     <  1:2921042/93‑1 (MQ=255)

      cAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATc                                                                                   <  2:719644/93‑1 (MQ=255)

        ggggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCACTccc                                                                                 >  2:988483/1‑93 (MQ=255)

        ggggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATccc                                                                                 >  1:1271705/1‑93 (MQ=255)

         gggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCa                                                                                <  2:1955968/93‑1 (MQ=255)

          ggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCAt                                                                               <  2:762631/93‑1 (MQ=255)

          ggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCAt                                                                               <  2:853083/93‑1 (MQ=255)

          ggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCAt                                                                               <  2:876720/93‑1 (MQ=255)

          ggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCAt                                                                               <  1:4628865/93‑1 (MQ=255)

            cGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgc                                                                             <  2:2159982/93‑1 (MQ=255)

            cGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgc                                                                             >  2:2080688/1‑93 (MQ=255)

             gAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcg                                                                            >  1:2718165/1‑93 (MQ=255)

              aGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcgc                                                                           <  2:3908686/93‑1 (MQ=255)

              aGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcgc                                                                           <  1:2199567/93‑1 (MQ=255)

                 cAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGcgcgcg                                                                        >  1:660840/1‑93 (MQ=255)

                   aaCCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcggg                                                                      <  1:1453685/93‑1 (MQ=255)

                     ccAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcg                                                                    <  1:2501573/93‑1 (MQ=255)

                      cAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcgc                                                                   >  2:3642498/1‑93 (MQ=255)

                       aGTGCCGATGCGCCCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcgcg                                                                  <  1:1091053/93‑1 (MQ=255)

                        gTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcgcgg                                                                 >  1:837270/1‑93 (MQ=255)

                        gTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcgcgg                                                                 <  1:4271522/93‑1 (MQ=255)

                         ggCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCgcggc                                                                <  2:4880/92‑1 (MQ=255)

                          gCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCgcggcg                                                               <  1:4538835/93‑1 (MQ=255)

                          gCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGAGCGGCCATCCCATGCGCGCGGGCgcggcg                                                               >  1:824893/1‑93 (MQ=255)

                           ccGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGt                                                              >  2:2943652/1‑93 (MQ=255)

                             gATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTaa                                                            >  1:242642/1‑93 (MQ=255)

                              aTGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAag                                                           >  2:1917758/1‑93 (MQ=255)

                               tGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAaga                                                          <  2:3312120/93‑1 (MQ=255)

                                  gcTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaa                                                       >  2:3392954/1‑93 (MQ=255)

                                   cTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaaa                                                      <  1:320094/93‑1 (MQ=255)

                                    tCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTGTTCCGGGGCGGCAATCCCATGCGCGCGGGCGCGGCGGAAGAGaaaa                                                     >  2:1831517/1‑93 (MQ=255)

                                      atatTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAAcc                                                   <  2:1116711/93‑1 (MQ=255)

                                       tatTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCa                                                  >  2:146908/1‑93 (MQ=255)

                                       tatTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCa                                                  >  2:1447703/1‑93 (MQ=255)

                                        atTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAt                                                 <  1:2851864/93‑1 (MQ=255)

                                        atTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGGAAGAGAAAACCAt                                                 >  2:584600/1‑93 (MQ=255)

                                         ttCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAtt                                                >  2:2141160/1‑93 (MQ=255)

                                         ttCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAtt                                                >  1:3038534/1‑93 (MQ=255)

                                         ttCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAtt                                                >  2:1368222/1‑93 (MQ=255)

                                          tccctctCCTTTCTTTTTCCCATTTCAAACAGGCGGTTG‑TTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGa                                              <  1:2105941/90‑1 (MQ=255)

                                          tCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTg                                               >  2:4459568/1‑93 (MQ=255)

                                            aCTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGat                                             <  2:2340719/93‑1 (MQ=255)

                                            aCTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGat                                             <  1:1060711/93‑1 (MQ=255)

                                            aCTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGAGCGGCGTAAGAGAAAACCATTGat                                             >  2:4596872/1‑93 (MQ=255)

                                             ctctCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGata                                            <  2:3592075/93‑1 (MQ=255)

                                             ctctCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGata                                            >  2:305839/1‑93 (MQ=255)

                                             ctctCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGata                                            >  1:3823352/1‑93 (MQ=255)

                                             ctctCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGata                                            <  1:2966976/93‑1 (MQ=255)

                                              tctcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGatat                                           <  2:3562576/93‑1 (MQ=255)

                                              tctcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGatat                                           >  2:2572112/1‑93 (MQ=255)

                                               ctcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGAtata                                          <  1:4431693/93‑1 (MQ=255)

                                               ctcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGAtata                                          >  2:3436359/1‑93 (MQ=255)

                                               ctcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGAtata                                          <  2:1632351/93‑1 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAg                                         >  1:4234687/1‑93 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGGTAGAGAAAACCATTGATATAg                                         >  2:2432678/1‑93 (MQ=255)

                                                 ccTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGt                                        >  1:1652535/1‑93 (MQ=255)

                                                 ccTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGt                                        >  2:4311199/1‑93 (MQ=255)

                                                  ctttctttTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTc                                       >  2:4294342/1‑93 (MQ=255)

                                                   tttctttTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCg                                      <  1:2677482/93‑1 (MQ=255)

                                                   tttctttTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCg                                      >  1:4261222/1‑93 (MQ=255)

                                                     tctttTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGAt                                    >  1:3952564/1‑93 (MQ=255)

                                                      ctttTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGAtt                                   <  1:2807184/93‑1 (MQ=255)

                                                       tttttACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGAttt                                  >  1:3432203/1‑93 (MQ=255)

                                                        ttttACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTc                                 <  1:3715262/93‑1 (MQ=255)

                                                        ttttACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTc                                 <  2:3233618/93‑1 (MQ=255)

                                                         tttACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCa                                >  2:3981693/1‑93 (MQ=255)

                                                            aCCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAgtg                             <  1:1405067/93‑1 (MQ=255)

                                                             ccATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAgtgt                            <  2:2850020/93‑1 (MQ=255)

                                                             ccATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAgtgt                            >  2:2566144/1‑93 (MQ=255)

                                                             ccATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAgtgt                            <  2:2300341/93‑1 (MQ=255)

                                                              cATTTCAAACAGTCGGGTGTTTTCCGGTGCGGCATTCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGtgtg                           <  2:3480818/93‑1 (MQ=255)

                                                              cATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGtgtg                           >  2:2824793/1‑93 (MQ=255)

                                                              cATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGtgtg                           >  2:2119022/1‑93 (MQ=255)

                                                               aTTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTgg                          <  1:2369660/93‑1 (MQ=255)

                                                                tttCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGgc                         >  2:1614523/1‑93 (MQ=255)

                                                                   cAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGgcgcg                      >  1:479887/1‑92 (MQ=255)

                                                                   cAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCa                      <  1:4570854/93‑1 (MQ=255)

                                                                     aaCAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAgc                    >  2:3126332/1‑93 (MQ=255)

                                                                     aaCAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAgc                    >  2:3182437/1‑93 (MQ=255)

                                                                      aCAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAgcg                   >  1:1580048/1‑93 (MQ=255)

                                                                         ggCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAgcgcgc                >  1:2197752/1‑93 (MQ=255)

                                                                           cGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGATAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCgg              >  2:617156/1‑93 (MQ=255)

                                                                           cGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCgg              <  1:3159849/93‑1 (MQ=255)

                                                                            gggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGa             >  1:504121/1‑93 (MQ=255)

                                                                             ggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGat            >  2:4410599/1‑93 (MQ=255)

                                                                             ggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGat            >  1:3101952/1‑93 (MQ=255)

                                                                             ggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGat            >  2:1918516/1‑93 (MQ=255)

                                                                               tgtTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGatat          >  2:2250654/1‑93 (MQ=255)

                                                                                gtTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATc         >  2:527456/1‑93 (MQ=255)

                                                                                   ttCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTg      <  1:1905113/93‑1 (MQ=255)

                                                                                   ttCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTg      >  1:1363209/1‑93 (MQ=255)

                                                                                    tCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGc     <  2:4498088/93‑1 (MQ=255)

                                                                                    tCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGc     >  1:3408521/1‑93 (MQ=255)

                                                                                    tCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGc     <  1:527562/93‑1 (MQ=255)

                                                                                       ggTGCGGCAAGCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAAc  <  1:3220137/93‑1 (MQ=255)

CACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAAC  >  NC_000913/442826‑443005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`