BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,772	C→T	C346C (TGC→TGT)	trkH →	TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,772	0	C	T	99.5%	667.9 / NA	193	C346C (TGC→TGT)	trkH	TrkH
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (75/117); total (76/117)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 222 total aligned reads displayed.

ACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCC  >  NC_000913/4031683‑4031861

aCAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGcc                                                                                         <  1:2810429/93‑1 (MQ=255)

aCAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGcc                                                                                         >  1:2087607/1‑93 (MQ=255)

 cAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCg                                                                                        <  1:409101/93‑1 (MQ=255)

 cAACCGCCGGGATTACAACTGACAGCATTGCCCGCTGGCCGCTTTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGAGGTTGTGCCg                                                                                        >  2:2352199/1‑93 (MQ=255)

   aCCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCggg                                                                                      <  1:2158545/93‑1 (MQ=255)

   aCCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGGTGTGGCggg                                                                                      >  2:2423748/1‑93 (MQ=255)

   aCCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGGCGTGCCggg                                                                                      >  1:1267877/1‑93 (MQ=255)

       ccgGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCaa                                                                                  <  1:3029634/93‑1 (MQ=255)

       ccgGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCaa                                                                                  <  1:259285/93‑1 (MQ=255)

        cgGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAAc                                                                                 <  2:1370990/93‑1 (MQ=255)

        cgGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGGTGTGCCGGGGCAAc                                                                                 >  2:1750553/1‑93 (MQ=255)

         gggTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACg                                                                                <  2:1324852/93‑1 (MQ=255)

           gTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACcgg                                                                              >  2:600385/1‑93 (MQ=255)

            tttACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACgggg                                                                             >  1:1580436/1‑92 (MQ=255)

            tttACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGc                                                                             >  1:2925441/1‑93 (MQ=255)

             ttACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCg                                                                            <  2:3172020/93‑1 (MQ=255)

              tACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCgg                                                                           <  2:1118347/93‑1 (MQ=255)

               aCAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGt                                                                          <  2:4083879/93‑1 (MQ=255)

                cAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTg                                                                         <  2:646225/93‑1 (MQ=255)

                 aaCTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGGCGGGGCAAAGgggggggg                                                                        >  2:380015/1‑93 (MQ=255)

                  aCTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑c                                                                      >  2:4608549/1‑93 (MQ=255)

                   cTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑cc                                                                     >  2:2274105/1‑93 (MQ=255)

                   cTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGGGG‑cc                                                                     >  1:1336021/1‑93 (MQ=255)

                   cTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGGCAACGGGCGGGGG‑cc                                                                     >  2:397590/1‑93 (MQ=255)

                      aCAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGa                                                                  <  2:3260940/93‑1 (MQ=255)

                       cAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGaa                                                                 <  2:774398/93‑1 (MQ=255)

                       cAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGGCAACGGGCGGTGG‑CCTgga                                                                 >  2:2905535/1‑91 (MQ=255)

                         gCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGGCGGGTCCACGGGCGGTGG‑CCTGAAgg                                                               >  1:1417798/1‑93 (MQ=255)

                         gCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGGGGTGG‑GCTGAAgg                                                               >  2:743938/1‑93 (MQ=255)

                         gCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGGTGTGCCGGGGCCACGGGCGGTGG‑GCTGAAgg                                                               >  2:3478711/1‑93 (MQ=255)

                          cATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGt                                                              <  1:363185/93‑1 (MQ=255)

                          cATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGt                                                              <  2:1492238/93‑1 (MQ=255)

                           tttGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTg                                                             <  2:3634698/92‑1 (MQ=255)

                           cttGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCAGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTg                                                             <  2:4385270/92‑1 (MQ=255)

                             tGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGAt                                                           <  1:4283105/93‑1 (MQ=255)

                              gCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGCTGTGCCGGGTCAACTGGCGGGGGACTTGAAgggatc                                                           >  2:2825959/1‑89 (MQ=255)

                               cccTCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATcc                                                         <  1:1332134/93‑1 (MQ=255)

                               cccGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATcc                                                         <  1:278223/93‑1 (MQ=255)

                                ccGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑ACTGAAGGTGATCCg                                                        >  1:3783423/1‑93 (MQ=255)

                                 cGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGc                                                       <  1:4649180/93‑1 (MQ=255)

                                 cGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCCGGTCAACGGGCGGTGG‑CCTGAATGTGATgcgc                                                       >  1:201953/1‑93 (MQ=255)

                                    tGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATc                                                    <  2:3773317/93‑1 (MQ=255)

                                    tGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATc                                                    <  2:297096/93‑1 (MQ=255)

                                      gCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCct                                                  >  1:1006786/1‑93 (MQ=255)

                                        cGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgc                                                <  2:2015247/93‑1 (MQ=255)

                                        cGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgc                                                <  2:2124914/93‑1 (MQ=255)

                                         gCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgct                                               <  1:2953335/93‑1 (MQ=255)

                                         gCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgct                                               >  2:1867609/1‑93 (MQ=255)

                                         gCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGACGGGGCAACGGGCGGTGG‑GCTGAAGGAGATGCTCATCctgct                                               >  1:1245813/1‑93 (MQ=255)

                                          ctctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATTCGCATCctgctg                                              >  2:2994921/1‑93 (MQ=255)

                                          ctctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCTGCATCctgctg                                              >  2:1806380/1‑93 (MQ=255)

                                          ctctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCACATCctgctg                                              >  1:3383134/1‑93 (MQ=255)

                                          ctctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGAGCGGTGG‑CCTGCAGGGGATCCGCATCctgctg                                              >  1:2307250/1‑93 (MQ=255)

                                          ctctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGGTGTGCCGGGTCAACCGGCGGTGG‑CCTGAAGGGGATCCGCATCccgctg                                              >  2:3069316/1‑93 (MQ=255)

                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCCACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgctgc                                             >  2:3308844/1‑93 (MQ=255)

                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgctgc                                             <  2:452369/93‑1 (MQ=255)

                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgctgc                                             >  2:4550304/1‑93 (MQ=255)

                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgctgc                                             >  2:502273/1‑93 (MQ=255)

                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgctgc                                             <  1:836983/93‑1 (MQ=255)

                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCctgctgc                                             >  1:4553039/1‑93 (MQ=255)

                                                ttGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGttt                                        >  2:548740/1‑93 (MQ=255)

                                                 tGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTa                                       <  1:4412280/93‑1 (MQ=255)

                                                    cGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGc                                    >  1:3276831/1‑93 (MQ=255)

                                                       tACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAgg                                 <  1:1515399/93‑1 (MQ=255)

                                                       tACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAgg                                 >  1:596087/1‑93 (MQ=255)

                                                        aCTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAggg                                <  2:4349645/93‑1 (MQ=255)

                                                         cTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAgggg                               <  2:2582122/93‑1 (MQ=255)

                                                         cTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAgggg                               <  2:2526222/93‑1 (MQ=255)

                                                         cTGCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAgggg                               <  2:2839034/93‑1 (MQ=255)

                                                           gCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGaa                             <  2:4240483/93‑1 (MQ=255)

                                                           gCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGaa                             >  1:4138025/1‑93 (MQ=255)

                                                           gCTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGaa                             >  1:3762410/1‑93 (MQ=255)

                                                            cTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAAc                            <  2:4683619/93‑1 (MQ=255)

                                                            cTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAAc                            <  2:1631312/93‑1 (MQ=255)

                                                            cTTTTATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAAc                            <  1:3982917/93‑1 (MQ=255)

                                                             ttttATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGGAcc                           >  1:276894/1‑93 (MQ=255)

                                                              tttATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCg                          >  2:369303/1‑93 (MQ=255)

                                                              tttATGTTCAGCCTCTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCg                          >  1:1834740/1‑93 (MQ=255)

                                                               ttATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGt                         <  1:1097845/93‑1 (MQ=255)

                                                               ttATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGt                         <  2:3960535/93‑1 (MQ=255)

                                                                tATGTTCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTTTTTAAGCAGGGGAACCGTg                        <  1:1937748/93‑1 (MQ=255)

                                                                    ttCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCt                    >  2:1342090/1‑93 (MQ=255)

                                                                    ttCAGCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCt                    >  1:3257994/1‑93 (MQ=255)

                                                                        gCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGaaa                <  1:1063106/93‑1 (MQ=255)

                                                                        gCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGaaa                <  1:1853057/93‑1 (MQ=255)

                                                                        gCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGaaa                <  2:1047260/93‑1 (MQ=255)

                                                                        gCCTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGaaa                <  1:3882381/93‑1 (MQ=255)

                                                                          cTTTATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGGGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACg              >  2:1960450/1‑93 (MQ=255)

                                                                           tttATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACgc             >  2:239073/1‑92 (MQ=255)

                                                                           tttATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGa             <  1:951852/93‑1 (MQ=255)

                                                                            ttATCGGCGGTTGTTCCGGGTCAACGGGCGGGGG‑CCTGAAGGGGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGGCGGCTGTAACGAc            >  2:3076716/1‑93 (MQ=255)

                                                                            ttATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGAc            <  2:4200589/93‑1 (MQ=255)

                                                                            ttATCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGAc            <  2:3889481/93‑1 (MQ=255)

                                                                               tCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTgg         <  2:3187812/93‑1 (MQ=255)

                                                                               tCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTgg         <  1:2209296/93‑1 (MQ=255)

                                                                               tCGGCGGTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGGTTAAGCAGGGGAACCGTGAGCTGAAACGACtcg         >  1:235426/1‑91 (MQ=255)

                                                                               acggcggTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTgg         <  1:3492482/92‑1 (MQ=255)

                                                                                 ggcggTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTg       <  2:1649467/93‑1 (MQ=255)

                                                                                    ggTTGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCAt    >  2:2924158/1‑93 (MQ=255)

                                                                                      ttGTGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATcc  <  2:3434779/93‑1 (MQ=255)

ACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGG‑CCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCC  >  NC_000913/4031683‑4031861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`