BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,045,192	C→T	A216V (GCC→GTC)	polA →	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,045,192	0	C	T	100.0%	774.7 / NA	215	A216V (GCC→GTC)	polA	DNA polymerase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (102/113); total (102/113)

Only 100 of 221 total aligned reads displayed.

CCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT  >  NC_000913/4045102‑4045281

 ctctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGa                                                                                        <  1:904909/93‑1 (MQ=255)

  tctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAg                                                                                       >  2:496696/1‑93 (MQ=255)

  tctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGAATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAg                                                                                       <  1:4451124/93‑1 (MQ=255)

    tGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGcc                                                                                     >  2:81915/1‑93 (MQ=255)

     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    >  1:1435102/1‑93 (MQ=255)

     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    >  2:1889597/1‑93 (MQ=255)

     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    >  1:2329904/1‑93 (MQ=255)

      aTAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAg                                                                                   <  1:3593042/93‑1 (MQ=255)

       tAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGa                                                                                  >  2:555358/1‑93 (MQ=255)

          cATTCCGGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaa                                                                               <  2:423571/93‑1 (MQ=255)

           aTTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaaa                                                                              >  1:454837/1‑93 (MQ=255)

            ttCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaaaa                                                                             >  1:3241543/1‑93 (MQ=255)

             tCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAAt                                                                            >  2:1286189/1‑93 (MQ=255)

                tGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGc                                                                         <  2:297853/93‑1 (MQ=255)

                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  1:2029422/93‑1 (MQ=255)

                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  1:517596/93‑1 (MQ=255)

                    gTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTggg                                                                     >  1:2455893/1‑93 (MQ=255)

                      aCCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGtt                                                                   >  1:2846708/1‑93 (MQ=255)

                      aCCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGtt                                                                   <  2:1563169/93‑1 (MQ=255)

                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              <  2:905306/93‑1 (MQ=255)

                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              <  1:2638334/93‑1 (MQ=255)

                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              <  1:2507605/93‑1 (MQ=255)

                           gCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCt                                                              >  1:1343962/1‑93 (MQ=255)

                             gTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTc                                                            <  2:1943707/93‑1 (MQ=255)

                             gTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTc                                                            >  1:4087064/1‑93 (MQ=255)

                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTcc                                                           >  2:1353053/1‑93 (MQ=255)

                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTcc                                                           <  2:2546324/93‑1 (MQ=255)

                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGCTGAGCTTcc                                                           >  2:691790/1‑93 (MQ=255)

                                ggTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGt                                                         >  2:1754148/1‑93 (MQ=255)

                                ggTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGt                                                         >  1:2626709/1‑93 (MQ=255)

                                ggTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGt                                                         <  2:4395521/93‑1 (MQ=255)

                                 gTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTg                                                        <  1:3523718/93‑1 (MQ=255)

                                   gAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgc                                                      >  1:320664/1‑93 (MQ=255)

                                    aaaaaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcg                                                     >  2:1162430/1‑93 (MQ=255)

                                     aaaaaCCGCGTAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcgc                                                    <  2:1237686/93‑1 (MQ=255)

                                     aaaaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcgc                                                    >  1:108545/1‑93 (MQ=255)

                                     aaaaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcgc                                                    >  2:3445841/1‑93 (MQ=255)

                                       aaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGa                                                  >  1:4128429/1‑93 (MQ=255)

                                           cgcgCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAAc                                              <  1:279131/93‑1 (MQ=255)

                                             cgcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGTGCTTCCGTGGCGCGAAAACaa                                            <  2:2983772/93‑1 (MQ=255)

                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           >  2:2694809/1‑93 (MQ=255)

                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           <  2:3261758/93‑1 (MQ=255)

                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           >  1:3891993/1‑93 (MQ=255)

                                               cAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATg                                          >  1:56642/1‑93 (MQ=255)

                                                 ggCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGc                                        >  1:2006631/1‑93 (MQ=255)

                                                 ggCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGc                                        <  2:3924692/93‑1 (MQ=255)

                                                 ggCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGc                                        >  1:1786399/1‑93 (MQ=255)

                                                  gCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCa                                       <  2:652987/93‑1 (MQ=255)

                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     >  2:1206821/1‑93 (MQ=255)

                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     <  1:2903699/93‑1 (MQ=255)

                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     >  1:1150957/1‑93 (MQ=255)

                                                    aTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGc                                     <  2:2030081/93‑1 (MQ=255)

                                                     ttGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCg                                    >  2:723570/1‑93 (MQ=255)

                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  <  2:107673/93‑1 (MQ=255)

                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  >  2:2513543/1‑93 (MQ=255)

                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  <  2:2136880/93‑1 (MQ=255)

                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  <  2:4431426/93‑1 (MQ=255)

                                                        ctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAg                                 <  2:3904674/93‑1 (MQ=255)

                                                         tgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGc                                <  1:2197104/93‑1 (MQ=255)

                                                          gcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCt                               >  2:27334/1‑93 (MQ=255)

                                                          gcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCt                               >  1:3600884/1‑93 (MQ=255)

                                                           cAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTc                              >  1:335270/1‑93 (MQ=255)

                                                              ggTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAg                           <  2:4149170/93‑1 (MQ=255)

                                                                tCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCa                         <  1:2702343/93‑1 (MQ=255)

                                                                tCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCa                         <  2:4631621/93‑1 (MQ=255)

                                                                   tGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCaaaa                      <  1:2477741/93‑1 (MQ=255)

                                                                   tGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCaaaa                      <  1:1508100/93‑1 (MQ=255)

                                                                   tGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCaaaa                      <  1:4160297/93‑1 (MQ=255)

                                                                    ggCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAc                     <  2:1813039/93‑1 (MQ=255)

                                                                     gCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACa                    <  2:2991147/93‑1 (MQ=255)

                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  <  2:3843605/93‑1 (MQ=255)

                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  <  2:3698682/93‑1 (MQ=255)

                                                                        gACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaag                 >  1:4659130/1‑93 (MQ=255)

                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  2:217238/93‑1 (MQ=255)

                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  2:1845880/93‑1 (MQ=255)

                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  1:4417092/93‑1 (MQ=255)

                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               <  1:10376/93‑1 (MQ=255)

                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               <  1:620081/93‑1 (MQ=255)

                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               >  1:3731959/1‑93 (MQ=255)

                                                                          cTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTACGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaa               <  2:2949735/93‑1 (MQ=255)

                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              >  1:1669951/1‑93 (MQ=255)

                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              <  2:2455544/93‑1 (MQ=255)

                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              <  2:1149255/93‑1 (MQ=255)

                                                                            ggATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGt             <  1:3711011/93‑1 (MQ=255)

                                                                            ggATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGt             >  1:4593341/1‑93 (MQ=255)

                                                                             gATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGtt            <  1:1536066/93‑1 (MQ=255)

                                                                              aTACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTg           >  2:4119884/1‑93 (MQ=255)

                                                                               tACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGc          >  2:491545/1‑93 (MQ=255)

                                                                               tACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGc          >  2:223742/1‑93 (MQ=255)

                                                                               tACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGc          <  1:306133/93‑1 (MQ=255)

                                                                                aCGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCt         <  1:3984079/93‑1 (MQ=255)

                                                                                   cTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAt      >  1:3127757/1‑93 (MQ=255)

                                                                                    tGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtc     >  2:2106019/1‑93 (MQ=255)

                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   >  2:2276417/1‑93 (MQ=255)

                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  1:195078/93‑1 (MQ=255)

                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  1:1176956/93‑1 (MQ=255)

                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  2:3207077/93‑1 (MQ=255)

                                                                                       aTGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctct  <  2:3134660/93‑1 (MQ=255)

                                                                                       aTGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctct  >  2:3405680/1‑93 (MQ=255)

                                                                                       aTGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctct  <  2:3726341/93‑1 (MQ=255)

CCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT  >  NC_000913/4045102‑4045281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`