BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,080,195	A→G	C49R (TGT→CGT)	fdoH ←	formate dehydrogenase‑O, iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,080,195	0	A	G	100.0%	726.7 / NA	232	C49R (TGT→CGT)	fdoH	formate dehydrogenase‑O, iron‑sulfur subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (124/108); total (124/108)

Only 100 of 238 total aligned reads displayed.

CGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGG  >  NC_000913/4080106‑4080284

 gTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGcc                                                                                       <  2:3055914/93‑1 (MQ=255)

 gTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGcc                                                                                       <  2:4071322/93‑1 (MQ=255)

    cACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCAcc                                                                                    >  2:236212/1‑93 (MQ=255)

    cACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCAcc                                                                                    >  2:4280277/1‑93 (MQ=255)

       gATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGa                                                                                 <  1:1352483/93‑1 (MQ=255)

       gATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGa                                                                                 <  2:1486391/93‑1 (MQ=255)

        aTTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAc                                                                                >  2:1360107/1‑93 (MQ=255)

        aTTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGAc                                                                                >  2:144320/1‑93 (MQ=255)

         tttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACa                                                                               >  2:818870/1‑93 (MQ=255)

         tttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACa                                                                               >  2:3622969/1‑93 (MQ=255)

          ttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAg                                                                              >  2:344026/1‑93 (MQ=255)

          ttGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAg                                                                              <  1:2189211/93‑1 (MQ=255)

           tGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAgg                                                                             >  2:1006831/1‑93 (MQ=255)

            ggCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGc                                                                            <  1:3959362/93‑1 (MQ=255)

             gcgcTTAAATCATTGGGGGTGTCGTACACCCCAATGTTATTACCGACGGTAGCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCt                                                                           <  2:1948358/93‑1 (MQ=38)

                 ttAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAc                                                                       >  1:497948/1‑93 (MQ=255)

                 ttAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTAc                                                                       >  1:2531117/1‑93 (MQ=255)

                  tAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACa                                                                      <  1:2136161/93‑1 (MQ=255)

                   aaaTCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAg                                                                     >  2:3890352/1‑93 (MQ=255)

                       cATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGa                                                                 <  2:4194610/93‑1 (MQ=255)

                        aTTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGAt                                                                >  2:4378720/1‑93 (MQ=255)

                        aTTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGAt                                                                >  2:1026152/1‑93 (MQ=255)

                         ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATa                                                               >  2:3921911/1‑93 (MQ=255)

                         ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATa                                                               >  1:3408553/1‑93 (MQ=255)

                         ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATa                                                               >  2:319133/1‑93 (MQ=255)

                         ttGGGGTTGTCGTACACCCCAATGTTATTCCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGTCGATa                                                               >  2:4328193/1‑93 (MQ=255)

                           tgggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACa                                                             <  1:2091092/92‑1 (MQ=255)

                           ggggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACa                                                             >  1:840804/1‑93 (MQ=255)

                            gggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAg                                                            <  2:1710305/93‑1 (MQ=255)

                              gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggt                                                          <  2:2254494/93‑1 (MQ=255)

                              gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggt                                                          <  1:1997106/93‑1 (MQ=255)

                              gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggt                                                          <  1:673976/93‑1 (MQ=255)

                               ttGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTTCCGCCGATACAggtg                                                         >  2:2166672/1‑93 (MQ=255)

                               ttGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggtg                                                         <  2:4552459/93‑1 (MQ=255)

                                 gTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAggtggt                                                       >  2:385849/1‑93 (MQ=255)

                                   cGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTaa                                                     <  2:4457988/93‑1 (MQ=255)

                                    gTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAAc                                                    <  2:2711182/93‑1 (MQ=255)

                                     tACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACg                                                   >  1:4609887/1‑93 (MQ=255)

                                      acacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGt                                                  <  1:4089531/93‑1 (MQ=255)

                                       cacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTc                                                 <  2:3750606/93‑1 (MQ=255)

                                        acCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCg                                                >  2:1559837/1‑93 (MQ=255)

                                        acCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGAGGTAACGTCg                                                >  2:2037187/1‑93 (MQ=255)

                                        acCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGACACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCg                                                >  1:700225/1‑93 (MQ=255)

                                         ccccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGa                                               <  1:1814417/93‑1 (MQ=255)

                                         ccccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGa                                               <  2:4538964/93‑1 (MQ=255)

                                          cccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGAt                                              <  2:2270491/93‑1 (MQ=255)

                                          cccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGAt                                              >  2:616403/1‑93 (MQ=255)

                                          cccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGAt                                              >  1:3627732/1‑93 (MQ=255)

                                           ccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATg                                             >  1:1435324/1‑93 (MQ=255)

                                           ccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATg                                             >  1:4417986/1‑93 (MQ=255)

                                           ccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATg                                             >  2:3805360/1‑93 (MQ=255)

                                             aaTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAg                                           >  2:481178/1‑93 (MQ=255)

                                              aTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGt                                          >  1:125684/1‑93 (MQ=255)

                                              aTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGt                                          >  1:3660392/1‑93 (MQ=255)

                                               tGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGtt                                         <  1:4414851/93‑1 (MQ=255)

                                                gTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGttt                                        >  1:3495926/1‑93 (MQ=255)

                                                 ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTc                                       >  2:29765/1‑93 (MQ=255)

                                                 ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTc                                       >  1:4667442/1‑93 (MQ=255)

                                                 ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTc                                       <  1:3273453/93‑1 (MQ=255)

                                                 gtATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTc                                       <  1:2045087/92‑1 (MQ=255)

                                                   aTTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGc                                     >  1:808791/1‑93 (MQ=255)

                                                     tGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCa                                   >  1:1915621/1‑93 (MQ=255)

                                                      gCCGACGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCAc                                  <  2:3243998/93‑1 (MQ=255)

                                                        cGACGGTATCGCGAATGTCCTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCActt                                >  2:4579794/1‑93 (MQ=255)

                                                          aCGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCActtct                              <  2:1845044/93‑1 (MQ=255)

                                                           cGGTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCACCActtctt                             <  2:710298/93‑1 (MQ=255)

                                                           cGGTATCGCGAATGTCGTCCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCActtctt                             <  2:4144697/93‑1 (MQ=255)

                                                             gTATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTcc                           <  1:3116134/93‑1 (MQ=255)

                                                              tATCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCt                          <  2:1936101/93‑1 (MQ=255)

                                                               aTCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTg                         >  2:2420615/1‑93 (MQ=255)

                                                               aTCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTg                         <  2:166007/93‑1 (MQ=255)

                                                                tCGCGAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTgg                        >  2:544890/1‑93 (MQ=255)

                                                                  gcgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGaa                      <  2:1084320/93‑1 (MQ=255)

                                                                   cgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAg                     >  1:1502577/1‑93 (MQ=255)

                                                                   cgAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAg                     <  2:344098/93‑1 (MQ=255)

                                                                    gAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGt                    >  2:1746254/1‑93 (MQ=255)

                                                                    gAATGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGt                    >  2:1152994/1‑93 (MQ=255)

                                                                     aaTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTc                   >  1:59246/1‑93 (MQ=255)

                                                                     aaTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTc                   >  1:2687204/1‑93 (MQ=255)

                                                                     aaTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTc                   >  1:3778115/1‑93 (MQ=255)

                                                                     aaTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTc                   >  1:3294675/1‑93 (MQ=255)

                                                                      aTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTcc                  >  2:915626/1‑93 (MQ=255)

                                                                      aTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTcc                  <  1:3925868/93‑1 (MQ=255)

                                                                      aTGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTcc                  <  1:2863623/93‑1 (MQ=255)

                                                                       tGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTccc                 >  1:104592/1‑93 (MQ=255)

                                                                       tGTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTccc                 <  1:417935/93‑1 (MQ=255)

                                                                        gTCGTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCcg                >  1:237012/1‑93 (MQ=255)

                                                                           gTTCCACTCTGACCGCGCCACCTGACAGGCTTTACAGCCGATACATGTGGTAACGTCCGTGAGTTTCGCCACTTCTTCCTGGAAGTCCCgcgc             >  1:2261973/1‑93 (MQ=21)

                                                                           gTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCgcgc             >  2:2588122/1‑93 (MQ=255)

                                                                           gTTCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCgcgc             >  2:3351306/1‑93 (MQ=255)

                                                                             tCCACTCTGAGCGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCt           >  2:2870377/1‑93 (MQ=255)

                                                                             tCCACTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCt           >  1:3137378/1‑93 (MQ=255)

                                                                               cACTCGGAACGCGCCACCTGACAGCCTTGACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGa         <  2:1786541/93‑1 (MQ=21)

                                                                                aCTCTGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCACGCGCCTGAg        >  1:1980897/1‑93 (MQ=255)

                                                                                 ctctGAACGCGCCCCCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAgg       <  1:4243673/93‑1 (MQ=255)

                                                                                  tctGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc      >  1:1933064/1‑93 (MQ=255)

                                                                                  tctGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc      <  2:2951736/93‑1 (MQ=255)

                                                                                    tGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc    >  1:3886425/1‑93 (MQ=255)

                                                                                    tGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc    >  1:1102093/1‑93 (MQ=255)

                                                                                    tGAACGCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGACTGAGgcgc    <  2:644276/93‑1 (MQ=255)

CGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGG  >  NC_000913/4080106‑4080284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`