BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,081,716	A→G	F563L (TTC→CTC)	fdoG ←	formate dehydrogenase‑O, major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,081,716	0	A	G	100.0%	661.7 / NA	208	F563L (TTC→CTC)	fdoG	formate dehydrogenase‑O, major subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (86/122); total (86/122)

Only 100 of 212 total aligned reads displayed.

AGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTT  >  NC_000913/4081626‑4081805

 gAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGa                                                                                        <  2:3271749/93‑1 (MQ=255)

 gAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGa                                                                                        <  2:276544/93‑1 (MQ=255)

  aaGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAg                                                                                       <  2:2936943/93‑1 (MQ=255)

    gTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGc                                                                                     <  2:3977149/93‑1 (MQ=255)

    gTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGc                                                                                     >  2:1785716/1‑93 (MQ=255)

     ttAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCa                                                                                    >  1:95184/1‑93 (MQ=255)

      tAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCaa                                                                                   <  2:4006257/93‑1 (MQ=255)

      tAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCaa                                                                                   <  1:1895398/93‑1 (MQ=255)

        gagGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACa                                                                                 >  1:3853984/1‑93 (MQ=255)

         agGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAg                                                                                <  1:3467118/93‑1 (MQ=255)

          ggTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAgg                                                                               <  1:653260/93‑1 (MQ=255)

           gTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAggg                                                                              <  2:3819954/93‑1 (MQ=255)

           gTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAggg                                                                              >  2:3047130/1‑93 (MQ=255)

             ttcagtgttcagtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGtt                                                                            <  2:4517620/93‑1 (MQ=255)

              tcagtgttcagtgGGTCGATGGTGACGAGGAACTTCAGTTTGGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCCACAGGGTTa                                                                           >  1:300383/1‑93 (MQ=255)

                   gttcagtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGcc                                                                      >  1:2225128/1‑93 (MQ=255)

                    ttcagtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGGTTTTGTTCGGGAGTGAGGCAACAGAGCTAAAGacc                                                                     >  2:1527788/1‑93 (MQ=255)

                       agtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTgg                                                                  >  2:2883871/1‑93 (MQ=255)

                       agtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTgg                                                                  <  1:2299946/93‑1 (MQ=255)

                        gtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGc                                                                 <  2:1345903/93‑1 (MQ=255)

                        gtgGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGc                                                                 <  1:1148376/93‑1 (MQ=255)

                          gggTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAg                                                               <  2:1281577/93‑1 (MQ=255)

                          gggTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAg                                                               <  1:421080/93‑1 (MQ=255)

                           ggTCGATGGTGACGAGGAACTTCAGTTGCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGa                                                              <  1:1696629/93‑1 (MQ=255)

                            gTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGat                                                             <  2:2006623/93‑1 (MQ=255)

                            gTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGat                                                             <  2:4108032/93‑1 (MQ=255)

                             tCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCTATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGata                                                            <  1:1423627/93‑1 (MQ=255)

                             tCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCACCAGGGTTAAAGCCCTGGCAGata                                                            <  2:587998/93‑1 (MQ=255)

                             tCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGata                                                            <  2:1202984/93‑1 (MQ=255)

                               gATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGAtata                                                          <  2:2457447/93‑1 (MQ=255)

                               gATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGAtata                                                          <  1:1815442/93‑1 (MQ=255)

                                 tGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGc                                                        >  1:4575692/1‑93 (MQ=255)

                                 tGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGc                                                        <  1:557469/93‑1 (MQ=255)

                                   gTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCACCAGGGTTAAAGCCCTGGCAGATATAGCCa                                                      >  2:2240377/1‑93 (MQ=255)

                                   gTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCa                                                      <  2:3869171/93‑1 (MQ=255)

                                     gACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt                                                    <  1:4237748/93‑1 (MQ=255)

                                     gACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCAtt                                                    <  2:713346/93‑1 (MQ=255)

                                       cGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGa                                                  <  2:3936608/93‑1 (MQ=255)

                                       cGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGa                                                  >  2:4285764/1‑93 (MQ=255)

                                        gAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGAc                                                 <  1:2771361/93‑1 (MQ=255)

                                        gAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGAc                                                 >  1:3143412/1‑93 (MQ=255)

                                         aGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGAcc                                                <  1:350257/93‑1 (MQ=255)

                                          ggAACTTCAGTTTCGACAGACAACCGATCGCTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGCCCt                                               <  1:642572/93‑1 (MQ=255)

                                          ggAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCt                                               <  1:2759577/93‑1 (MQ=255)

                                          ggAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCt                                               <  1:1098130/93‑1 (MQ=255)

                                          ggAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCt                                               <  1:3806231/93‑1 (MQ=255)

                                            aaCTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTg                                             >  2:1567600/1‑93 (MQ=255)

                                              cTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGcc                                           >  2:3229098/1‑93 (MQ=255)

                                              cTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGcc                                           >  1:2973224/1‑93 (MQ=255)

                                               ttCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGccc                                          >  2:3027437/1‑93 (MQ=255)

                                               ttCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGccc                                          <  2:112242/93‑1 (MQ=255)

                                               ttCAGTTTCGAAAGACAACCGATAACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGCTAAAGCCCTGGCAGATATAGCCATTGACCTTGcac                                          >  2:1483746/1‑91 (MQ=255)

                                                tCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCct                                         >  1:472441/1‑93 (MQ=255)

                                                  aGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCctct                                       >  2:2658223/1‑93 (MQ=255)

                                                   gTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCtt                                      <  1:2619077/93‑1 (MQ=255)

                                                     ttCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtc                                    <  1:3249643/93‑1 (MQ=255)

                                                     ttCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtc                                    <  2:230218/93‑1 (MQ=255)

                                                      tCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtca                                   <  2:3739360/93‑1 (MQ=255)

                                                        gacagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcatc                                 >  1:2476991/1‑93 (MQ=255)

                                                        gacagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcatc                                 <  2:1997341/93‑1 (MQ=255)

                                                        gacagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTCGCAGATATAGCCATTGACCTTGCCCTCTTtcatc                                 >  1:184989/1‑93 (MQ=255)

                                                         acagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcatca                                <  1:1411564/93‑1 (MQ=255)

                                                         acagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTtcatca                                <  2:3415421/93‑1 (MQ=255)

                                                          cagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTcatcat                               >  1:2576816/1‑93 (MQ=255)

                                                          cagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTcatcat                               >  2:4406589/1‑93 (MQ=255)

                                                          cagacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTcatcat                               >  2:2079988/1‑93 (MQ=255)

                                                           agacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCatcatc                              <  2:4667281/93‑1 (MQ=255)

                                                           agacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCatcatc                              <  2:817503/93‑1 (MQ=255)

                                                            gacaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAAATATAGCCATTGACCTTGCCCTCTTTCATCAtct                             >  2:3640093/1‑93 (MQ=255)

                                                             acaACCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCAtctc                            >  2:3585247/1‑93 (MQ=255)

                                                               aaCCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGa                          >  1:2159257/1‑93 (MQ=255)

                                                                aCCGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGaa                         <  2:1053258/93‑1 (MQ=255)

                                                                  cGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGt                       <  1:195577/93‑1 (MQ=255)

                                                                  cGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGt                       <  1:2431007/93‑1 (MQ=255)

                                                                  cGATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGt                       <  1:89781/93‑1 (MQ=255)

                                                                   gATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTa                      >  1:4261643/1‑93 (MQ=255)

                                                                   gATCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTa                      <  2:3846306/93‑1 (MQ=255)

                                                                    aTCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTAt                     <  2:165144/93‑1 (MQ=255)

                                                                     tCACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTAtt                    <  1:823158/93‑1 (MQ=255)

                                                                      cACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTg                   >  1:526355/1‑93 (MQ=255)

                                                                      cACTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTg                   <  2:2725837/93‑1 (MQ=255)

                                                                       aCTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGc                  <  1:679469/93‑1 (MQ=255)

                                                                       aCTTTGTTTTTGTTCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGc                  >  1:2375597/1‑93 (MQ=255)

                                                                        cTTTGTTTTTGTTCGGGAGTGAGGCAACAGGTTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCa                 <  1:981846/93‑1 (MQ=255)

                                                                         tttgtttttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAg                >  1:338632/1‑93 (MQ=255)

                                                                          ttgtttttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAgg               <  1:3415567/93‑1 (MQ=255)

                                                                           tgtttttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGa              <  2:4589064/93‑1 (MQ=255)

                                                                           tgtttttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGa              <  1:4313490/93‑1 (MQ=255)

                                                                             tttttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGAcg            >  2:3558437/1‑93 (MQ=255)

                                                                              ttttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGAcgt           <  2:2318459/93‑1 (MQ=255)

                                                                              ttttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGAcgt           >  1:1324414/1‑93 (MQ=255)

                                                                               tttgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGAcgtc          <  2:3115696/93‑1 (MQ=255)

                                                                                 tgttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGAcgtcgt        <  2:1166500/93‑1 (MQ=255)

                                                                                  gttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTa       <  1:4594186/93‑1 (MQ=255)

                                                                                   ttCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAg      >  1:3683455/1‑93 (MQ=255)

                                                                                    tCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGc     >  2:741798/1‑93 (MQ=255)

                                                                                    tCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGc     >  1:1000933/1‑93 (MQ=255)

                                                                                    tCGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGc     <  1:4027679/93‑1 (MQ=255)

                                                                                     cGGGAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCGTCATCTCGAAGCATTGCAGGACGGCGTAggc    >  2:2985814/1‑91 (MQ=38)

                                                                                       ggAGTGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCtt  <  1:4462292/93‑1 (MQ=255)

AGAAGTTAGAGGTTTCAGTGTTCAGTGGGTCGATGGTGACGAGGAACTTCAGTTTCGACAGACAACCGATCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTT  >  NC_000913/4081626‑4081805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`