BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,105,759	A→G	I210V (ATC→GTC)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,105,759	0	A	G	99.6%	705.9 / ‑4.8	229	I210V (ATC→GTC)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (105/123); total (105/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 238 total aligned reads displayed.

GCTGCGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTATCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACC  >  NC_000913/4105669‑4105848

  tGCGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTcgc                                                                                       <  1:3734009/93‑1 (MQ=255)

  tGCGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTcgc                                                                                       >  1:1752275/1‑93 (MQ=255)

      gCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGaaa                                                                                   >  2:1271397/1‑93 (MQ=255)

       ccATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAg                                                                                  <  1:4312473/93‑1 (MQ=255)

       ccATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAg                                                                                  <  1:2148473/93‑1 (MQ=255)

       ccATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAg                                                                                  >  2:3926475/1‑93 (MQ=255)

         aTTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGt                                                                                <  2:3423954/93‑1 (MQ=255)

         aTTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGt                                                                                >  2:1539641/1‑93 (MQ=255)

          ttGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTa                                                                               >  2:4230389/1‑93 (MQ=255)

          ttGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTa                                                                               >  1:2049113/1‑93 (MQ=255)

             ccGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaaa                                                                            <  2:2349001/93‑1 (MQ=255)

             ccGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaaa                                                                            <  1:897973/93‑1 (MQ=255)

              cGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaaaa                                                                           <  2:1149694/93‑1 (MQ=255)

               ggTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaaaaa                                                                          <  1:801805/93‑1 (MQ=255)

               ggTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaaaaa                                                                          <  1:2383390/93‑1 (MQ=255)

               ggTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaaaaa                                                                          <  2:928915/93‑1 (MQ=255)

                gTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAac                                                                         <  1:1942486/93‑1 (MQ=255)

                 tGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAaca                                                                        <  2:4042814/93‑1 (MQ=255)

                 tGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAaca                                                                        >  1:2617135/1‑93 (MQ=255)

                 tGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAaca                                                                        >  1:1618005/1‑93 (MQ=255)

                   gCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACAcg                                                                      >  1:3520929/1‑93 (MQ=255)

                   gCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACAcg                                                                      >  1:1210251/1‑93 (MQ=255)

                     tgtgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACAcgcg                                                                    >  2:1728985/1‑93 (MQ=255)

                       tgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGAt                                                                  <  2:1109704/93‑1 (MQ=255)

                        gAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATc                                                                 <  2:229785/93‑1 (MQ=255)

                        gAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATc                                                                 <  2:2124679/93‑1 (MQ=255)

                        gAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATc                                                                 <  1:1787524/93‑1 (MQ=255)

                        gAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATc                                                                 >  1:2067142/1‑93 (MQ=255)

                        gAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATc                                                                 <  1:2511067/93‑1 (MQ=255)

                         aaTTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCg                                                                <  1:1385692/93‑1 (MQ=255)

                         aaTTCGTTGTGGTTCCGGAAGGTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAAGTAAAAAACACGCGATCg                                                                >  1:1103036/1‑93 (MQ=255)

                          aTTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGt                                                               >  2:140497/1‑93 (MQ=255)

                          aTTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGt                                                               >  1:3859739/1‑93 (MQ=255)

                          aTTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGt                                                               <  2:1795942/93‑1 (MQ=255)

                          aTTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGt                                                               <  1:399690/93‑1 (MQ=255)

                           ttCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTg                                                              >  2:3359300/1‑93 (MQ=255)

                            tCGTTGTGGTTGCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTgg                                                             <  1:215722/93‑1 (MQ=255)

                            tCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTgg                                                             <  2:4587378/93‑1 (MQ=255)

                             cGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGc                                                            <  1:1161459/93‑1 (MQ=255)

                              gTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCg                                                           >  1:3031659/1‑93 (MQ=255)

                               ttGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGa                                                          <  1:3415511/93‑1 (MQ=255)

                                 gtgGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGAtt                                                        >  1:2674091/1‑93 (MQ=255)

                                 gtgGTTCCGGAAGTTGAATGCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGAtt                                                        <  2:4141103/93‑1 (MQ=255)

                                  tgGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTa                                                       >  2:776958/1‑93 (MQ=255)

                                    gTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTAcc                                                     >  2:1670124/1‑93 (MQ=255)

                                     gtCCGGAAGTTGAATTCAGCCGTGAAGACCTGGCAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCg                                                    >  2:2145514/2‑93 (MQ=255)

                                       ccGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGaa                                                  <  2:3600271/93‑1 (MQ=255)

                                         ggAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGcaca                                                >  2:2416816/1‑93 (MQ=255)

                                         ggAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACa                                                >  2:3849426/1‑93 (MQ=255)

                                         ggAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACa                                                >  2:3753601/1‑93 (MQ=255)

                                         ggAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACa                                                >  1:103009/1‑93 (MQ=255)

                                           aaGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACata                                              >  2:1063913/1‑93 (MQ=255)

                                           aaGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACata                                              <  2:3518445/93‑1 (MQ=255)

                                            aGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACatat                                             >  1:610958/1‑93 (MQ=255)

                                               tGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtg                                          <  2:920660/93‑1 (MQ=255)

                                               tGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtg                                          >  1:2278139/1‑93 (MQ=255)

                                               tGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtg                                          <  2:592138/93‑1 (MQ=255)

                                                gAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtgt                                         <  1:4631348/93‑1 (MQ=255)

                                                gAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtgt                                         <  1:4208858/93‑1 (MQ=255)

                                                  aTTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGa                                       <  1:2131671/93‑1 (MQ=255)

                                                   ttCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGAt                                      <  1:4499210/93‑1 (MQ=255)

                                                      aGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGtt                                   >  1:4119668/1‑93 (MQ=255)

                                                        ccGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGa                                 <  1:4138460/93‑1 (MQ=255)

                                                         cGTGAAGACCTGGTAACCGAAATCAAAGCGGGTGCCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGAc                                <  2:4558764/93‑1 (MQ=255)

                                                         cGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGAc                                >  1:3345626/1‑93 (MQ=255)

                                                          gTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACg                               >  2:3123487/1‑93 (MQ=255)

                                                            gAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTgaagaa                             >  2:3555622/1‑93 (MQ=255)

                                                            gAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGaa                             <  2:3049464/93‑1 (MQ=255)

                                                            gAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGaa                             <  1:1880530/93‑1 (MQ=255)

                                                             aaGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAAc                            >  1:995726/1‑93 (MQ=255)

                                                               gACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTg                          <  1:1550070/93‑1 (MQ=255)

                                                               gACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTg                          <  2:1755006/93‑1 (MQ=255)

                                                                 ccTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGgc                        <  1:3878334/93‑1 (MQ=255)

                                                                  cTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGgcg                       >  2:540930/1‑93 (MQ=255)

                                                                  cTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGgcg                       >  2:1312392/1‑93 (MQ=255)

                                                                   tGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGgcgc                      >  2:2779435/1‑93 (MQ=255)

                                                                   tGGTAAACGAAATCAAAGCGGGGGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAAATGgggc                      >  2:492058/1‑93 (MQ=255)

                                                                    ggTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCa                     <  2:4415866/93‑1 (MQ=255)

                                                                    ggTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCa                     <  1:1091425/93‑1 (MQ=255)

                                                                    ggTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCa                     <  2:1419921/93‑1 (MQ=255)

                                                                    ggTAAACGAAATCAAAGCGGGGGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCa                     >  1:1162449/1‑93 (MQ=255)

                                                                     gTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCAt                    <  1:743166/93‑1 (MQ=255)

                                                                     gTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCAt                    >  2:3092256/1‑93 (MQ=255)

                                                                     gTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCAt                    >  2:1600338/1‑93 (MQ=255)

                                                                      tAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCAtt                   <  1:4005530/93‑1 (MQ=255)

                                                                      tAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCAtt                   <  1:1412425/93‑1 (MQ=255)

                                                                        aaCGAAATCAAAGCGGGTGTGGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCAttta                 >  2:680210/1‑92 (MQ=255)

                                                                        aaCGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTc                 <  2:2594064/93‑1 (MQ=255)

                                                                         aCGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCa                <  2:2166953/93‑1 (MQ=255)

                                                                         aCGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCa                >  1:322043/1‑93 (MQ=255)

                                                                         aCGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCa                <  1:2835361/93‑1 (MQ=255)

                                                                          agAAATCAAAGCGGGTGTCGCGAAAAGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCAt               <  2:1030500/92‑1 (MQ=255)

                                                                           gAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATc              >  1:4074414/1‑93 (MQ=255)

                                                                           gAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATc              >  1:2868049/1‑93 (MQ=255)

                                                                                 aaaGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaaa        >  2:1926008/1‑93 (MQ=255)

                                                                                 aaaGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaaa        >  1:4671160/1‑93 (MQ=255)

                                                                                   aGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaaaga      <  1:3581577/93‑1 (MQ=255)

                                                                                    gCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaaagaa     <  2:3865501/93‑1 (MQ=255)

                                                                                      gggTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAAc   >  2:190788/1‑93 (MQ=255)

                                                                                       ggTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAAcc  <  2:3974825/93‑1 (MQ=255)

GCTGCGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTATCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACC  >  NC_000913/4105669‑4105848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`