Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,120,575 T→C T115T (ACA→ACG)  ftsN ← essential cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,120,5750TC99.5% 664.6 / NA 210T115T (ACA→ACG) ftsNessential cell division protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (111/98);  minor base G (1/0);  total (112/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAAT  >  NC_000913/4120485‑4120664
                                                                                          |                                                                                         
cGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGgcgt                                                                                         <  1:263406/93‑1 (MQ=255)
cGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGgcgt                                                                                         >  1:2080668/1‑93 (MQ=255)
cGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGgcgt                                                                                         >  2:1364247/1‑93 (MQ=255)
  tCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCa                                                                                       >  1:2275789/1‑93 (MQ=255)
  tCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCa                                                                                       <  2:3729320/93‑1 (MQ=255)
  tCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCa                                                                                       >  1:3972371/1‑93 (MQ=255)
  tCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCa                                                                                       <  2:1566297/93‑1 (MQ=255)
    tGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGt                                                                                     >  2:4201447/1‑93 (MQ=255)
    tGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGt                                                                                     <  2:396155/93‑1 (MQ=255)
    tGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGt                                                                                     >  1:1210011/1‑93 (MQ=255)
     gTTCATTCCACGGAACTTGAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGtt                                                                                    <  2:1133149/93‑1 (MQ=255)
        cATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCt                                                                                 <  2:4662281/93‑1 (MQ=255)
         aTTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCGTTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTc                                                                                <  2:2124661/93‑1 (MQ=255)
         aTTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTc                                                                                >  1:1504255/1‑93 (MQ=255)
            ccACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCgg                                                                             >  2:1645928/1‑93 (MQ=255)
            ccACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCgg                                                                             <  2:2224717/93‑1 (MQ=255)
              ccGGAACTTCAACCAGCTGCGTTGGCTGCTGGCTCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCg                                                                           <  1:3499321/92‑1 (MQ=255)
              aCGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCg                                                                           >  2:4536195/1‑93 (MQ=255)
              aCGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGACTGCATTTGTTCAAGAAGCTGAAGTTGTTCTGGCGTCAGTTGCTCCGGCg                                                                           >  2:3796723/1‑93 (MQ=255)
                ggAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGtt                                                                         >  1:1086247/1‑93 (MQ=255)
                   aCTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttc                                                                      >  2:122673/1‑93 (MQ=255)
                    cTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttca                                                                     <  2:3940214/93‑1 (MQ=255)
                    cTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttca                                                                     <  2:1937054/93‑1 (MQ=255)
                     ttCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttcac                                                                    >  1:2228412/1‑93 (MQ=255)
                     ttCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttcac                                                                    >  1:4492103/1‑93 (MQ=255)
                      tCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttcact                                                                   <  2:4565167/93‑1 (MQ=255)
                         tccAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGAAGTTGTTCTGGGGTCAGTTGCTCCGGCCCTttcacttca                                                                >  2:1817256/2‑93 (MQ=255)
                         aCCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttcacttca                                                                >  1:4380834/1‑93 (MQ=255)
                          ccAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTttcacttcac                                                               >  1:3255044/1‑93 (MQ=255)
                             gCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTcaccac                                                            >  2:4655686/1‑93 (MQ=255)
                              cTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCaccacc                                                           <  1:1103484/93‑1 (MQ=255)
                                   ttGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAg                                                      >  2:2303592/1‑93 (MQ=255)
                                      gctgctGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAg                                                   <  2:740288/93‑1 (MQ=255)
                                        tgctgGCGCATATGAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGt                                                 <  1:4628150/93‑1 (MQ=255)
                                        tgctgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGt                                                 <  2:1739717/93‑1 (MQ=255)
                                          ctgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTc                                               <  1:63999/93‑1 (MQ=255)
                                             gcgcATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCtgt                                            <  1:1345800/93‑1 (MQ=255)
                                             gcgcATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCtgt                                            <  1:118350/93‑1 (MQ=255)
                                              cgcATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCtgtg                                           <  2:960428/93‑1 (MQ=255)
                                               gcATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTgg                                          <  1:4554324/93‑1 (MQ=255)
                                                cATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTggg                                         >  2:590121/1‑93 (MQ=255)
                                                cATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTggg                                         >  2:3437269/1‑93 (MQ=255)
                                                cATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTggg                                         >  1:1543466/1‑93 (MQ=255)
                                                 atatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGgc                                        >  1:1469093/1‑93 (MQ=255)
                                                 atatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGgc                                        >  2:1081220/1‑93 (MQ=255)
                                                 atatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGgc                                        <  2:1297593/93‑1 (MQ=255)
                                                  tatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGgcg                                       >  1:1000521/1‑93 (MQ=255)
                                                   atCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGgcgc                                      >  2:3526882/1‑93 (MQ=255)
                                                     cAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGcgcac                                    <  1:3829215/93‑1 (MQ=255)
                                                       gCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGcgcacgc                                  >  1:1804661/1‑93 (MQ=255)
                                                       gCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGcgcacgc                                  >  1:1154725/1‑93 (MQ=255)
                                                        ccTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGcgcacgca                                 >  2:680054/1‑93 (MQ=255)
                                                        ccTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGcgcacgca                                 <  1:220504/93‑1 (MQ=255)
                                                         cTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCgcacgcac                                >  1:4269865/1‑93 (MQ=255)
                                                         cTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCgcacgcac                                >  1:3420336/1‑93 (MQ=255)
                                                         cTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCgcacgcac                                >  1:1769425/1‑93 (MQ=255)
                                                         cTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCgcacgcac                                >  2:4511984/1‑93 (MQ=255)
                                                         cTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCgcacgcac                                <  1:2781476/93‑1 (MQ=255)
                                                          tGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACt                               >  2:4105528/1‑93 (MQ=255)
                                                          tGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACt                               >  2:4333366/1‑93 (MQ=255)
                                                          tGCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACt                               <  2:275593/93‑1 (MQ=255)
                                                           gCATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTc                              >  1:1976231/1‑93 (MQ=255)
                                                            cATTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTcc                             <  1:3322693/93‑1 (MQ=255)
                                                             aTTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTccc                            >  2:2850553/1‑93 (MQ=255)
                                                             aTTTGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTccc                            >  1:2383758/1‑93 (MQ=255)
                                                              tttGTTCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCg                           >  1:4297162/1‑93 (MQ=255)
                                                                  ttCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTg                       <  2:2721556/93‑1 (MQ=255)
                                                                  ttCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTg                       >  2:2914823/1‑93 (MQ=255)
                                                                  ttCAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTg                       >  2:2398443/1‑93 (MQ=255)
                                                                    cAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGc                     >  2:2025554/1‑93 (MQ=255)
                                                                    cAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGc                     <  1:618217/93‑1 (MQ=255)
                                                                    cAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGc                     <  2:1407786/93‑1 (MQ=255)
                                                                    cAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGc                     <  2:3568358/93‑1 (MQ=255)
                                                                    cAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGc                     <  2:3710464/93‑1 (MQ=255)
                                                                    cAAGAAGCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGc                     <  2:3909492/93‑1 (MQ=255)
                                                                      agaagCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCCGCTGGCgg                   >  1:461968/1‑92 (MQ=255)
                                                                       gaagCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGAc                  >  2:4057560/1‑93 (MQ=255)
                                                                         agCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACtt                <  1:3070613/93‑1 (MQ=255)
                                                                         agCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACtt                >  1:944501/1‑93 (MQ=255)
                                                                         agCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGAGGGCGCACGCACTCCCGGCTGGCGACtt                >  1:3589554/1‑93 (MQ=255)
                                                                          gCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACttt               >  2:4001153/1‑93 (MQ=255)
                                                                          gCTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACttt               <  2:55130/93‑1 (MQ=255)
                                                                           cTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTc              <  1:2424686/93‑1 (MQ=255)
                                                                           cTGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTc              <  1:1199733/93‑1 (MQ=255)
                                                                            tGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTcc             >  1:3315520/1‑93 (MQ=255)
                                                                            tGACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTcc             <  2:836171/93‑1 (MQ=255)
                                                                             gACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCa            >  1:3161444/1‑93 (MQ=255)
                                                                             gACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCa            <  1:4178933/93‑1 (MQ=255)
                                                                             gACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCa            >  1:1422665/1‑93 (MQ=255)
                                                                             gACGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCa            <  1:4047294/93‑1 (MQ=255)
                                                                              aCGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAg           >  1:818490/1‑93 (MQ=255)
                                                                               cGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGc          <  1:2998235/93‑1 (MQ=255)
                                                                               cGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGc          <  1:2882533/93‑1 (MQ=255)
                                                                               cGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGc          >  2:3613605/1‑93 (MQ=255)
                                                                               cGTTGTTCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGGTCTGTGGGCGCACGCACTCCCGGCTGGCGGCTTTCCAGc          >  2:1245333/1‑93 (MQ=255)
                                                                                 ttgttCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGATCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGctc        >  2:888635/1‑93 (MQ=255)
                                                                                  tgttCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGGTCTGTGGGCGCACGCACTCCCGGCTGGCGACCTTGCAGctct       >  2:3635421/1‑93 (MQ=255)
                                                                                   gttCTGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCtt      <  1:1624973/93‑1 (MQ=255)
                                                                                       tGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAAt  >  2:3226210/1‑93 (MQ=255)
                                                                                       tGGCGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAAt  <  2:2531630/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAAT  >  NC_000913/4120485‑4120664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑