BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	465,393	T→C	T382A (ACC→GCC)	ybaE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	465,393	0	T	C	99.3%	432.1 / ‑5.3	140	T382A (ACC→GCC)	ybaE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (57/82); total (58/82)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 146 total aligned reads displayed.

AAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTC  >  NC_000913/465303‑465482

aaaCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGa                                                                                         >  2:2197222/1‑93 (MQ=255)

aaaCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGa                                                                                         >  2:3981193/1‑93 (MQ=255)

 aaCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGAt                                                                                        >  1:3460152/1‑93 (MQ=255)

  aCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATc                                                                                       >  2:703275/1‑93 (MQ=255)

  aCCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATc                                                                                       >  2:654467/1‑93 (MQ=255)

   ccAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCa                                                                                      >  1:4576083/1‑93 (MQ=255)

   ccAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCa                                                                                      >  1:3379476/1‑93 (MQ=255)

     aGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAgg                                                                                    <  2:939584/93‑1 (MQ=255)

     aGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAgg                                                                                    >  1:4516365/1‑93 (MQ=255)

       cGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGtt                                                                                  <  1:397240/93‑1 (MQ=255)

       cGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGtt                                                                                  <  1:3206882/93‑1 (MQ=255)

        gTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGttt                                                                                 <  1:4306420/93‑1 (MQ=255)

        gTCAAGGTTTTCGGTAGTTTGACTTCATCCCGTACCTGCCCATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGttt                                                                                 >  2:1504334/1‑93 (MQ=255)

         tCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGtttt                                                                                <  2:2377141/93‑1 (MQ=255)

         tCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGtttt                                                                                >  1:3142816/1‑93 (MQ=255)

          cAAGTTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTtc                                                                               <  1:2945431/93‑1 (MQ=255)

          cAAGGTTTTCGGTTGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTtc                                                                               <  1:548078/93‑1 (MQ=255)

            aGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTtctc                                                                             <  1:4146172/93‑1 (MQ=255)

            aGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTtctc                                                                             <  2:2759409/93‑1 (MQ=255)

              gTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCg                                                                           <  2:4541243/93‑1 (MQ=255)

              gTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCg                                                                           >  2:356423/1‑93 (MQ=255)

               ttttCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGa                                                                          >  2:819747/1‑93 (MQ=255)

                tttCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAc                                                                         >  1:2469585/1‑93 (MQ=255)

                tttCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGAc                                                                         <  1:4311359/93‑1 (MQ=255)

                tttCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGAc                                                                         <  2:2624259/93‑1 (MQ=255)

                tttCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGAc                                                                         <  2:3450587/93‑1 (MQ=255)

                  tCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACtt                                                                       <  2:2200941/93‑1 (MQ=255)

                  tCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACtt                                                                       >  2:2993584/1‑93 (MQ=255)

                   cGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTGTCTCCGACTTc                                                                      >  1:430742/1‑93 (MQ=255)

                     gTATTTTGACTACAGCCGGTACCTGCCAATGCGCAATAGTCCAGCATGGCAGTAATGCATGCCTGGCGGCAATCAGGTTTACTCCTACTTCTa                                                                    <  2:4009853/93‑1 (MQ=255)

                     gTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTa                                                                    <  1:454298/93‑1 (MQ=255)

                     gTAGTTTGACGTCATCCGTTACCTTCCAATGCCGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTa                                                                    <  1:3498893/93‑1 (MQ=255)

                      tAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTaa                                                                   <  1:1461115/93‑1 (MQ=255)

                      tAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTaa                                                                   >  1:1244596/1‑93 (MQ=255)

                       aGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAAc                                                                  <  2:4113438/93‑1 (MQ=255)

                        gTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACg                                                                 >  1:952871/1‑93 (MQ=255)

                        gTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACg                                                                 <  2:1776208/93‑1 (MQ=255)

                             aCTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGt                                                            >  1:2215317/1‑93 (MQ=255)

                             aCTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGt                                                            >  2:4624621/1‑93 (MQ=255)

                             aCTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGt                                                            <  2:187995/93‑1 (MQ=255)

                             aCTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGt                                                            <  2:3835027/93‑1 (MQ=255)

                              cTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGta                                                           <  1:2859503/93‑1 (MQ=255)

                              cTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGta                                                           <  2:1680073/93‑1 (MQ=255)

                               ttCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaa                                                          >  2:2419411/1‑93 (MQ=255)

                               ttCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaa                                                          >  2:3632232/1‑93 (MQ=255)

                               ttCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaa                                                          >  1:301050/1‑93 (MQ=255)

                                tCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaat                                                         <  1:122409/93‑1 (MQ=255)

                                tCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGATTTCTCCGACTTCTAACGTTTGtaat                                                         >  2:2891875/1‑93 (MQ=255)

                                  ctCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaataa                                                       <  2:76142/92‑1 (MQ=255)

                                  aTCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaataa                                                       <  2:3984868/93‑1 (MQ=255)

                                  aTCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaataa                                                       <  2:4522779/93‑1 (MQ=255)

                                  aTCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGtaataa                                                       <  1:1134813/93‑1 (MQ=255)

                                    ccGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTGATAAAc                                                     <  2:1374186/93‑1 (MQ=255)

                                     cGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAAcc                                                    <  2:875286/93‑1 (MQ=255)

                                        tACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGa                                                 <  2:1744681/93‑1 (MQ=255)

                                        tACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGa                                                 <  2:1120582/93‑1 (MQ=255)

                                         cccTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGAt                                                <  2:3630972/92‑1 (MQ=255)

                                         aCCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGAt                                                >  1:3473273/1‑93 (MQ=255)

                                           cTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATtg                                              <  1:1316886/93‑1 (MQ=255)

                                             gacAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATtgat                                            <  1:2267867/91‑1 (MQ=255)

                                               cAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATtgatga                                          <  1:316172/93‑1 (MQ=255)

                                                aaTGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaa                                         <  2:1473266/93‑1 (MQ=255)

                                                aaTGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaa                                         <  2:1635216/93‑1 (MQ=255)

                                                aaTGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaa                                         <  1:1677063/93‑1 (MQ=255)

                                                 aTGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaat                                        <  2:929102/93‑1 (MQ=255)

                                                  tGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaata                                       >  1:4266915/1‑93 (MQ=255)

                                                  tGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATAaata                                       <  2:1236294/93‑1 (MQ=255)

                                                   gCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaataa                                      <  2:1742290/93‑1 (MQ=255)

                                                    cGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGaataat                                     >  1:4268838/1‑93 (MQ=255)

                                                     ggAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATg                                    <  1:604497/93‑1 (MQ=255)

                                                     ggAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGCTTGTAATAAACCGGATTGATGAATAATg                                    >  1:3955879/1‑93 (MQ=255)

                                                       aaTAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGga                                  <  2:3832788/93‑1 (MQ=255)

                                                       aaTAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGga                                  <  1:2587858/93‑1 (MQ=255)

                                                        aTAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGgag                                 >  1:787840/1‑93 (MQ=255)

                                                        aTAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGgag                                 >  2:1946666/1‑93 (MQ=255)

                                                         tAGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGgaga                                >  2:287085/1‑93 (MQ=255)

                                                          aGTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGAt                               <  1:2732424/93‑1 (MQ=255)

                                                           gTCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATc                              <  2:2511895/93‑1 (MQ=255)

                                                            tCCAGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCa                             >  1:2142044/1‑93 (MQ=255)

                                                               aGCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACtt                          >  1:2523203/1‑93 (MQ=255)

                                                                gCCTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACttt                         >  1:2388455/1‑93 (MQ=255)

                                                                  cTGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTc                       >  2:267526/1‑93 (MQ=255)

                                                                   tGGCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcg                      >  1:2650029/1‑93 (MQ=255)

                                                                   cggCAGTCATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcg                      <  1:3460316/92‑1 (MQ=255)

                                                                    ggCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcgc                     <  2:3373573/93‑1 (MQ=255)

                                                                    ggCAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcgc                     <  2:3403781/93‑1 (MQ=255)

                                                                      cAGTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCgcgc                   >  1:1531981/1‑93 (MQ=255)

                                                                        gTAATGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCt                 <  1:685028/93‑1 (MQ=255)

                                                                           aTGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGcc              <  2:2675739/93‑1 (MQ=255)

                                                                            tGCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCa             >  2:1779004/1‑93 (MQ=255)

                                                                             gCATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCag            >  2:4028168/1‑93 (MQ=255)

                                                                              cATGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCaga           <  1:3838643/93‑1 (MQ=255)

                                                                               aTGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCagag          <  2:2637025/93‑1 (MQ=255)

                                                                               aTGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCagag          >  1:1189388/1‑93 (MQ=255)

                                                                                tGACTGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAgg         <  1:1683909/93‑1 (MQ=255)

                                                                                    tGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGgaga     <  1:2009458/93‑1 (MQ=255)

                                                                                    tGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGgaga     >  2:2357997/1‑93 (MQ=255)

                                                                                    tGGCGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTCCCAGAGGgagg     >  2:3531573/1‑92 (MQ=255)

                                                                                      gcggcgATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGt   <  2:223591/93‑1 (MQ=255)

                                                                                       cGGCGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTc  <  1:3062573/93‑1 (MQ=255)

AAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTC  >  NC_000913/465303‑465482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`