BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,140,312	G→A	M68I (ATG→ATA)	frwC →	PTS system fructose‑like enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,140,312	0	G	A	100.0%	893.9 / NA	244	M68I (ATG→ATA)	frwC	PTS system fructose‑like enzyme IIC component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (134/110); total (134/110)

Only 100 of 248 total aligned reads displayed.

GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401

gttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGt                                                                                         <  2:2324846/93‑1 (MQ=255)

   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      <  2:597978/93‑1 (MQ=255)

   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      >  1:3013365/1‑93 (MQ=255)

   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      <  1:2993514/93‑1 (MQ=255)

    tATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCt                                                                                     <  2:1631731/93‑1 (MQ=255)

    tATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCt                                                                                     >  1:916107/1‑93 (MQ=255)

     aTGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCtt                                                                                    <  1:1985558/93‑1 (MQ=255)

         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                <  2:941282/93‑1 (MQ=255)

           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                              >  1:108680/1‑93 (MQ=255)

           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                              <  2:3708246/93‑1 (MQ=255)

            aGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGc                                                                             <  2:2116616/93‑1 (MQ=255)

            aGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGc                                                                             >  1:2130390/1‑93 (MQ=255)

             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            >  1:3832432/1‑93 (MQ=255)

              gcgcAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAg                                                                           <  2:4549361/93‑1 (MQ=255)

                 cAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGt                                                                        >  1:2921085/1‑93 (MQ=255)

                  aGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGGGTa                                                                       >  2:3118992/1‑93 (MQ=255)

                   gTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAc                                                                      >  1:3680417/1‑93 (MQ=255)

                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                     >  1:2590486/1‑93 (MQ=255)

                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTTCATc                                                                   <  2:4379630/93‑1 (MQ=255)

                       cGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCg                                                                  >  1:4033820/1‑93 (MQ=255)

                        ggATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 >  1:4533796/1‑93 (MQ=255)

                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                >  2:1069495/1‑93 (MQ=255)

                          aTGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGtt                                                               <  2:2873903/93‑1 (MQ=255)

                           tGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                              >  2:1820631/1‑93 (MQ=255)

                           tGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                              >  2:2586415/1‑93 (MQ=255)

                            gCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAt                                                             <  1:3528928/93‑1 (MQ=255)

                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        >  1:2692605/1‑93 (MQ=255)

                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        <  1:3452105/93‑1 (MQ=255)

                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  2:564092/1‑93 (MQ=255)

                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       <  2:1014423/93‑1 (MQ=255)

                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  1:2916654/1‑93 (MQ=255)

                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  2:872805/1‑93 (MQ=255)

                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  1:2402567/1‑93 (MQ=255)

                                   ccGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTg                                                      <  2:2149113/93‑1 (MQ=255)

                                    cGATCCAAATCTGAAAAAACTGTTTGCTATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     >  2:297540/1‑93 (MQ=255)

                                    cGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     >  2:3241628/1‑93 (MQ=255)

                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:576038/1‑93 (MQ=255)

                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:17023/1‑93 (MQ=255)

                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:3355177/93‑1 (MQ=255)

                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:3582240/93‑1 (MQ=255)

                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  >  2:3553269/1‑93 (MQ=255)

                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:1512383/93‑1 (MQ=255)

                                          aaaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCg                                               >  1:4158356/1‑93 (MQ=255)

                                           aaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGt                                              >  1:2251217/1‑93 (MQ=255)

                                            aTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCACAGCGtt                                             >  2:1405068/1‑93 (MQ=255)

                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           >  2:2030054/1‑93 (MQ=255)

                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           <  2:2041926/93‑1 (MQ=255)

                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          >  1:3722220/1‑93 (MQ=255)

                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          <  1:1192379/93‑1 (MQ=255)

                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          >  1:1815928/1‑93 (MQ=255)

                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgc                                         >  2:2677261/1‑93 (MQ=255)

                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgc                                         <  1:3189281/93‑1 (MQ=255)

                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgc                                         <  2:1219967/93‑1 (MQ=255)

                                                 aaaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcg                                        >  1:1046371/1‑93 (MQ=255)

                                                 aaaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcg                                        >  1:2941810/1‑93 (MQ=255)

                                                 aaaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATACTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcg                                        >  1:2509765/1‑93 (MQ=255)

                                                   aaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCt                                      <  1:658934/93‑1 (MQ=255)

                                                    aaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTg                                     >  1:177499/1‑93 (MQ=255)

                                                     aaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTgg                                    >  2:478959/1‑93 (MQ=255)

                                                        tGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCATCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTc                                 <  1:3295048/93‑1 (MQ=255)

                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                >  2:4462401/1‑93 (MQ=255)

                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                >  1:1054970/1‑93 (MQ=255)

                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                >  1:447143/1‑93 (MQ=255)

                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               <  1:3883367/93‑1 (MQ=255)

                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               >  2:711126/1‑93 (MQ=255)

                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               <  2:2489045/93‑1 (MQ=255)

                                                            tGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTg                             >  2:506460/1‑93 (MQ=255)

                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  2:1728526/93‑1 (MQ=255)

                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          >  2:4331344/1‑93 (MQ=255)

                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  1:4296653/93‑1 (MQ=255)

                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  1:905478/93‑1 (MQ=255)

                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  2:4246736/1‑93 (MQ=255)

                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  1:4451854/1‑93 (MQ=255)

                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  2:4164912/1‑93 (MQ=255)

                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  2:4407860/93‑1 (MQ=255)

                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  2:3970193/1‑93 (MQ=255)

                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTCGCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  2:2460838/93‑1 (MQ=255)

                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  1:4586952/93‑1 (MQ=255)

                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  1:2857717/93‑1 (MQ=255)

                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  1:2821994/93‑1 (MQ=255)

                                                                   ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      <  1:3575701/93‑1 (MQ=255)

                                                                     cGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCgg                    >  1:2344820/1‑93 (MQ=255)

                                                                     cGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCgg                    <  2:1950253/93‑1 (MQ=255)

                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  1:2463943/1‑93 (MQ=255)

                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  2:1410620/1‑93 (MQ=255)

                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  2:284132/1‑93 (MQ=255)

                                                                         gCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGcc                <  1:1960004/93‑1 (MQ=255)

                                                                           gggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTg              <  1:138485/93‑1 (MQ=255)

                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             >  2:4056116/1‑93 (MQ=255)

                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             <  1:146567/93‑1 (MQ=255)

                                                                             gCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTggg            >  2:4640814/1‑93 (MQ=255)

                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          <  2:1198296/93‑1 (MQ=255)

                                                                                 gACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTgg        <  1:323917/93‑1 (MQ=255)

                                                                                   cGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGTGTTGGTa      >  2:2347936/1‑93 (MQ=255)

                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  1:3916208/1‑93 (MQ=255)

                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  2:668062/1‑93 (MQ=255)

                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  1:940729/1‑93 (MQ=255)

                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    <  2:342964/93‑1 (MQ=255)

                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    >  2:3291848/1‑93 (MQ=255)

                                                                                      tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   <  1:1707500/93‑1 (MQ=255)

GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`