BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,853	G→A	V178I (GTT→ATT)	nusG →	NusG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,853	0	G	A	99.6%	873.2 / NA	242	V178I (GTT→ATT)	nusG	NusG
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (114/127); minor base T (0/1); total (114/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       <  2:200947/93‑1 (MQ=255)

  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       <  1:802879/93‑1 (MQ=255)

  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       >  1:864061/1‑93 (MQ=255)

     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                    >  1:4246441/1‑93 (MQ=255)

      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   <  1:4029125/93‑1 (MQ=255)

       aagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAg                                                                                  >  1:3662651/1‑93 (MQ=255)

        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 >  2:3057213/1‑93 (MQ=255)

        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 >  1:4567371/1‑93 (MQ=255)

         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                <  1:3388634/93‑1 (MQ=255)

          tGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCt                                                                               >  2:3848939/1‑93 (MQ=255)

           ggATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTa                                                                              >  1:3092842/1‑93 (MQ=255)

           ggATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTa                                                                              <  2:244284/93‑1 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                             >  1:4099105/1‑93 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                             <  2:4590957/93‑1 (MQ=255)

                aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCa                                                                         >  1:1959644/1‑93 (MQ=255)

                 caagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAg                                                                        >  2:1483792/3‑93 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  1:593657/93‑1 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       >  2:308017/1‑93 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       >  1:3292490/1‑93 (MQ=255)

                   agaAATCTCGTTTGAAAGTTTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCg                                                                      <  2:1732317/93‑1 (MQ=255)

                   agaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCg                                                                      <  1:1171656/93‑1 (MQ=255)

                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     <  2:2295177/93‑1 (MQ=255)

                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   >  1:2584655/1‑93 (MQ=255)

                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   >  1:3589814/1‑93 (MQ=255)

                        tctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaa                                                                 >  1:1038584/1‑93 (MQ=255)

                            gTCTGAAATTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             <  1:3209060/93‑1 (MQ=255)

                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             >  2:3266412/1‑93 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAg                                                            <  2:824884/93‑1 (MQ=255)

                              cggAAAGTTTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGTTTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  2:1239388/91‑1 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           >  1:4461360/1‑93 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  2:2942605/93‑1 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  1:2698268/93‑1 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgc                                                           <  2:1366182/93‑1 (MQ=255)

                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  1:2770189/1‑93 (MQ=255)

                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  2:4208349/1‑93 (MQ=255)

                                 aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggc                                                        <  1:3315221/93‑1 (MQ=255)

                                  aaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggcg                                                       >  2:3753711/1‑93 (MQ=255)

                                   aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGa                                                      >  2:3970239/1‑93 (MQ=255)

                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     >  2:4054161/1‑93 (MQ=255)

                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    <  1:1130869/93‑1 (MQ=255)

                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    <  1:3968980/93‑1 (MQ=255)

                                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                  >  1:2923613/1‑93 (MQ=255)

                                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                  >  1:1794878/1‑93 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  2:3559836/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  1:3789740/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                >  2:1960451/1‑93 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  1:1306493/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                >  2:63922/1‑93 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               >  2:2478606/1‑93 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               <  1:2456363/93‑1 (MQ=255)

                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             <  2:22192/93‑1 (MQ=255)

                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             >  1:1410586/1‑93 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  1:521426/1‑93 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  2:1020053/1‑93 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            <  1:2708821/93‑1 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            <  1:3938150/93‑1 (MQ=255)

                                              cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                           >  1:906255/1‑93 (MQ=255)

                                              cTATCTACGGTCGTGCGGCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                           <  1:934583/93‑1 (MQ=255)

                                                aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGca                                         <  1:3471512/93‑1 (MQ=255)

                                                aTCTTCGGGCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAACCAGCGATGAAAAAAGCGGCGATTTAATCGCTGca                                         >  2:737613/1‑93 (MQ=255)

                                                 tCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                        >  2:1538795/1‑93 (MQ=255)

                                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcaca                                       >  2:1322921/1‑93 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      >  1:119085/1‑93 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  2:447931/93‑1 (MQ=255)

                                                    tCGCTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                     <  1:2067616/93‑1 (MQ=255)

                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  2:2790174/93‑1 (MQ=255)

                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  1:151008/93‑1 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:3854220/1‑93 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:2736097/1‑93 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:520172/1‑93 (MQ=255)

                                                        tCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 <  2:2553448/93‑1 (MQ=255)

                                                        tCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 <  1:3020919/93‑1 (MQ=255)

                                                        tCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 >  1:312662/1‑93 (MQ=255)

                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  2:1513507/93‑1 (MQ=255)

                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  1:2217991/93‑1 (MQ=255)

                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              >  1:4633850/1‑93 (MQ=255)

                                                             cGACCCCGGTAGAGCTGGACTTCAGCCTGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                            <  1:3085289/93‑1 (MQ=255)

                                                             cGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                            <  1:3149562/93‑1 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          >  2:4281696/1‑93 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       <  2:2820026/93‑1 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       >  2:3445112/1‑93 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       <  2:4333486/93‑1 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTACCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  1:16121/93‑1 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  1:395831/93‑1 (MQ=255)

                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  1:1874377/93‑1 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     <  2:161965/93‑1 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     <  1:1218958/93‑1 (MQ=255)

                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    <  2:3756991/93‑1 (MQ=255)

                                                                        gagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                 >  2:2781197/1‑93 (MQ=255)

                                                                         agCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                >  2:3675127/1‑93 (MQ=255)

                                                                           cTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc              >  1:2312310/1‑93 (MQ=255)

                                                                            tGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg             <  2:2652904/93‑1 (MQ=255)

                                                                             ggACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgc            >  1:1730431/1‑93 (MQ=255)

                                                                                 ttCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCt        <  1:415347/93‑1 (MQ=255)

                                                                                  tCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt       >  1:1020437/1‑93 (MQ=255)

                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      >  2:167825/1‑93 (MQ=255)

                                                                                    aGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtttt     >  1:4390807/1‑93 (MQ=255)

                                                                                      ccAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt   >  2:1905618/1‑93 (MQ=255)

                                                                                      ccAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt   <  1:1590974/93‑1 (MQ=255)

                                                                                       cAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt  <  2:1146668/93‑1 (MQ=255)

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`