BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	477,192	A→G	L219P (CTG→CCG)	ylaB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	477,192	0	A	G	99.4%	538.9 / ‑3.8	169	coding (656/1557 nt)	ylaB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (80/88); minor base . (1/0); total (81/88)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 173 total aligned reads displayed.

AACCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCAGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCGC  >  NC_000913/477102‑477279

aaCCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCggg                                                                                       >  1:2305294/1‑93 (MQ=255)

 aCCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGa                                                                                      >  2:3222419/1‑93 (MQ=255)

 aCCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGa                                                                                      <  1:572052/93‑1 (MQ=255)

   cAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGata                                                                                    >  1:1822312/1‑93 (MQ=255)

    aaaTAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGatat                                                                                   <  1:3261032/93‑1 (MQ=255)

    aaaTAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGatat                                                                                   >  1:1352146/1‑93 (MQ=255)

    aaaTAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGatat                                                                                   <  1:1674417/93‑1 (MQ=255)

     aaTAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATc                                                                                  >  1:1288615/1‑93 (MQ=255)

      aTAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCg                                                                                 <  1:555896/93‑1 (MQ=255)

      aTAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCg                                                                                 >  1:193114/1‑93 (MQ=255)

        aaaGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTa                                                                               >  2:3153372/1‑93 (MQ=255)

        aaaGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTa                                                                               <  2:2628660/93‑1 (MQ=255)

         aaGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGCGATATCGtca                                                                              >  2:461222/1‑91 (MQ=255)

          aGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTaaa                                                                             <  2:1672331/93‑1 (MQ=255)

           gACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAAt                                                                            <  1:213659/93‑1 (MQ=255)

           gACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAAt                                                                            <  2:3189262/93‑1 (MQ=255)

            aCTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATg                                                                           <  1:2766042/93‑1 (MQ=255)

            aCTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATg                                                                           <  1:4177455/93‑1 (MQ=255)

             cTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGa                                                                          <  2:1474071/93‑1 (MQ=255)

              ttcccGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGAt                                                                         <  1:1762943/90‑1 (MQ=255)

                 ccGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTcc                                                                      <  2:2434631/93‑1 (MQ=255)

                  cGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCa                                                                     <  2:566002/93‑1 (MQ=255)

                  cGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCa                                                                     <  2:149445/93‑1 (MQ=255)

                   gATGTCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCat                                                                    <  1:3533556/93‑1 (MQ=255)

                        cAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTAttt                                                               >  1:3244229/1‑93 (MQ=255)

                        cAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTAttt                                                               <  2:2845869/93‑1 (MQ=255)

                        cAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTAttt                                                               >  2:64010/1‑93 (MQ=255)

                        cAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTAttt                                                               >  1:4090896/1‑93 (MQ=255)

                          gCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTc                                                             >  1:3706579/1‑93 (MQ=255)

                            cTTTCTGCCACATTTTCGTCGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGa                                                           >  2:4576738/1‑93 (MQ=255)

                            cTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGa                                                           <  1:3549429/93‑1 (MQ=255)

                            cTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGa                                                           <  2:3684097/93‑1 (MQ=255)

                             tttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGat                                                          <  1:498118/93‑1 (MQ=255)

                             tttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGat                                                          >  2:1665069/1‑93 (MQ=255)

                             tttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGat                                                          >  1:1049203/1‑93 (MQ=255)

                             tttCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGat                                                          <  1:297795/93‑1 (MQ=255)

                                 tGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGc                                                      <  1:4419410/93‑1 (MQ=255)

                                 tGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGc                                                      <  2:2187474/93‑1 (MQ=255)

                                    aaCATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCa                                                   <  1:2842631/93‑1 (MQ=255)

                                    aaCATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCa                                                   >  2:1052109/1‑93 (MQ=255)

                                    aaCATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGAATTCATTATTTTCGATATGCTCa                                                   >  2:3616873/1‑93 (MQ=255)

                                     aCATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCAc                                                  >  2:3561562/1‑93 (MQ=255)

                                      cATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTGCATTATTTTCGATATGATAAcc                                                 >  2:2737602/1‑93 (MQ=255)

                                      cATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCAcc                                                 <  2:2134874/93‑1 (MQ=255)

                                      cATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCAcc                                                 <  2:3623276/93‑1 (MQ=255)

                                       aTTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCg                                                >  2:4164239/1‑93 (MQ=255)

                                       aTTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCg                                                >  2:2434019/1‑93 (MQ=255)

                                         tttCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATTGTAAATGATTCCATTATTTTCGATATGCTCACCggg                                              >  2:4295736/1‑93 (MQ=255)

                                           tCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGgtg                                            <  1:700351/93‑1 (MQ=255)

                                           tCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGgtg                                            >  1:1837415/1‑93 (MQ=255)

                                            cGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGgtgt                                           <  1:3969848/93‑1 (MQ=255)

                                             gTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGtt                                          <  2:1961018/93‑1 (MQ=255)

                                              ttGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGttt                                         >  1:3458320/1‑93 (MQ=255)

                                               tGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGtttt                                        >  1:4048556/1‑93 (MQ=255)

                                                gAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGttttt                                       <  2:859122/93‑1 (MQ=255)

                                                 aaGCCCATTTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGtttttt                                      >  2:4550261/1‑93 (MQ=255)

                                                 aaGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGtttttt                                      <  2:1576726/93‑1 (MQ=255)

                                                 aaGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGtttttt                                      >  2:4011665/1‑93 (MQ=255)

                                                 aaGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGtttttt                                      <  2:4005169/93‑1 (MQ=255)

                                                  aGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGttttttt                                     >  1:3227217/1‑92 (MQ=255)

                                                    cccATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTa                                   >  1:2919949/1‑93 (MQ=255)

                                                     ccATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAg                                  >  2:2916373/1‑93 (MQ=255)

                                                     ccATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAg                                  >  1:2867238/1‑93 (MQ=255)

                                                      cATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGc                                 >  2:1585553/1‑93 (MQ=255)

                                                      cATGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGc                                 >  1:1262112/1‑93 (MQ=255)

                                                       aTGTGATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATAGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGcc                                >  1:2513332/1‑93 (MQ=255)

                                                         gtgATGATCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTg                              <  1:2696871/93‑1 (MQ=255)

                                                          tgatgaTCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTgg                             <  2:4276550/93‑1 (MQ=255)

                                                           gatggtCGAAAGTTTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCTCCGGGTGTTTTTTGTCGGCTGGt                            <  2:1450566/93‑1 (MQ=255)

                                                            atgatCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGta                           <  2:1165077/93‑1 (MQ=255)

                                                             tgatCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaa                          <  1:3611438/93‑1 (MQ=255)

                                                              gatCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaat                         <  2:3242975/93‑1 (MQ=255)

                                                                tCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaataa                       >  2:2574599/1‑93 (MQ=255)

                                                                tCGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGtaataa                       <  2:2301871/93‑1 (MQ=255)

                                                                 cGAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTaataat                      <  2:1729902/93‑1 (MQ=255)

                                                                  gAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAAtt                     >  1:3346129/1‑93 (MQ=255)

                                                                  gAAATATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAAtt                     >  1:4344791/1‑93 (MQ=255)

                                                                   aaaTATTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATCCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTc                    >  2:749875/1‑93 (MQ=255)

                                                                     atatTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTccc                  >  1:2584044/1‑93 (MQ=255)

                                                                     atatTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGAGTTTTTTGTAGCCTGGTAATAATTccc                  >  2:1131011/1‑93 (MQ=255)

                                                                      tatTCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGTGTGTTTTTTGCAGCCTGGTAATAATTCCCt                 <  2:4526139/93‑1 (MQ=255)

                                                                        ttCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGa               <  1:753493/93‑1 (MQ=255)

                                                                         tCATCTCCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAg              >  1:888713/1‑93 (MQ=255)

                                                                         tCACCTCCGGTAAGGGCGAGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAg              <  2:2703783/93‑1 (MQ=255)

                                                                          cATCTCCGGTAAGGGAGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTgcgc             >  2:1824542/1‑93 (MQ=255)

                                                                            tctcCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCaa           >  1:1126764/1‑93 (MQ=255)

                                                                              tcCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAAtt         <  2:3389450/93‑1 (MQ=255)

                                                                              tcCGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGAGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAAtt         >  1:4074161/1‑93 (MQ=255)

                                                                                cGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTc       <  1:1029664/93‑1 (MQ=255)

                                                                                cGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTc       <  2:743593/93‑1 (MQ=255)

                                                                                cGGTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTc       <  2:1272522/93‑1 (MQ=255)

                                                                                  gTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCAt     <  1:3487442/93‑1 (MQ=255)

                                                                                  gTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCAt     >  1:1988326/1‑93 (MQ=255)

                                                                                  gTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCAt     <  2:2352999/93‑1 (MQ=255)

                                                                                  gTAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCAt     >  2:80412/1‑93 (MQ=255)

                                                                                   tAAGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATc    <  2:3809356/93‑1 (MQ=255)

                                                                                    aaGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCg   >  2:1947284/1‑93 (MQ=255)

                                                                                    aaGGGC‑GGATATCGTAAATGATTCCATTATTTTCGATATGCTCAACGGGTGTTTTTTGTAGCATGGTAATAATTCCCTGAGCAATTTCATCgc  >  2:3014146/1‑93 (MQ=255)

                                                                                     aGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCgc  <  2:1298276/93‑1 (MQ=255)

                                                                                     aGGGCGGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCgc  >  2:2014285/1‑93 (MQ=255)

AACCAAATAAAGACTTGCCGATGCCAGCCTTTCTGCAACATTTTCGTTGAAGCCCATGTGATGATCGAAATATTCATCTCCGGTAAGGGCAGGATATCGTAAATGATTCCATTATTTTCGATATGCTCACCGGGTGTTTTTTGTAGCCTGGTAATAATTCCCTGAGCAATTTCATCGC  >  NC_000913/477102‑477279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`