Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,203,421 A→G V30A (GTT→GCT)  purD ← phosphoribosylglycinamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,203,4210AG99.5% 583.4 / ‑4.8 184V30A (GTT→GCT) purDphosphoribosylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (106/77);  total (107/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

Only 100 of 208 total aligned reads displayed.

GCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAACAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATC  >  NC_000913/4203331‑4203510
                                                                                          |                                                                                         
 cGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGcagcaa                                                                                        >  1:92216/1‑93 (MQ=255)
 cGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGcagcaa                                                                                        >  1:505529/1‑93 (MQ=255)
  gAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaa                                                                                       >  2:2798778/1‑93 (MQ=255)
   aaaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaaa                                                                                      <  2:3503697/93‑1 (MQ=255)
   aaaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAAAGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaaa                                                                                      >  1:2329178/1‑93 (MQ=255)
    aaTCCAACAGCGCCGGGATATCGGTCACTCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaaaa                                                                                     <  1:4396360/93‑1 (MQ=255)
    aaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaaaa                                                                                     <  1:537935/93‑1 (MQ=255)
    aaTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCaaaaa                                                                                     >  1:2403635/1‑93 (MQ=255)
     aTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAAc                                                                                    >  2:4271271/1‑93 (MQ=255)
     aTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAAc                                                                                    >  2:2535588/1‑93 (MQ=255)
     aTCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAAc                                                                                    <  1:4600426/93‑1 (MQ=255)
      tCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACa                                                                                   >  2:1868557/1‑93 (MQ=255)
      tCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACa                                                                                   >  2:1598556/1‑93 (MQ=255)
       ccAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAg                                                                                  <  2:830896/93‑1 (MQ=255)
       ccAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAg                                                                                  <  1:1724826/93‑1 (MQ=255)
        cAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGt                                                                                 >  1:1354096/1‑93 (MQ=255)
         aaCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtc                                                                                <  2:3785398/93‑1 (MQ=255)
         aaCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtc                                                                                >  2:277467/1‑93 (MQ=255)
         aaCAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGtc                                                                                <  1:4116413/93‑1 (MQ=255)
               gcCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAAcc                                                                          <  2:21010/93‑1 (MQ=255)
                ccGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCa                                                                         >  1:1099213/1‑93 (MQ=255)
                ccGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCa                                                                         <  2:391558/93‑1 (MQ=255)
                 cGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAg                                                                        <  1:1990407/93‑1 (MQ=255)
                  gggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgc                                                                       <  1:2765711/93‑1 (MQ=255)
                   ggATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcg                                                                      >  2:3051838/1‑93 (MQ=255)
                    gATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcgg                                                                     <  1:2971273/93‑1 (MQ=255)
                    gATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcgg                                                                     >  1:849734/1‑93 (MQ=255)
                     atatCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgcggc                                                                    <  2:4186709/93‑1 (MQ=255)
                     atatCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAgccgc                                                                    >  2:3374399/1‑93 (MQ=255)
                       atCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGa                                                                  >  2:40520/1‑93 (MQ=255)
                        tCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGAc                                                                 <  2:1850762/93‑1 (MQ=255)
                        tCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGAc                                                                 <  1:1753762/93‑1 (MQ=255)
                           gTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTgg                                                              <  2:1217806/93‑1 (MQ=255)
                           gTCACGCCAATAGCAACGCTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACtcg                                                              >  1:162154/1‑91 (MQ=255)
                            tCACGCCAATAGCAACGTTTTGCAGCGCGGGTTACAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTggg                                                             >  2:631996/1‑93 (MQ=255)
                              aCGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGcc                                                           >  1:3807315/1‑93 (MQ=255)
                                 ccAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCt                                                        <  2:57208/93‑1 (MQ=255)
                                  cAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCtt                                                       >  1:3968888/1‑93 (MQ=255)
                                  cAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCtt                                                       >  2:564570/1‑93 (MQ=255)
                                  cAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCtt                                                       <  2:1391146/93‑1 (MQ=255)
                                   aaTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCttt                                                      >  1:1571876/1‑93 (MQ=255)
                                    aTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTc                                                     <  1:1491673/93‑1 (MQ=255)
                                    aTAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTc                                                     >  1:3335974/1‑93 (MQ=255)
                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    >  1:4264562/1‑93 (MQ=255)
                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    >  2:3286854/1‑93 (MQ=255)
                                     tAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTcc                                                    <  1:3496294/93‑1 (MQ=255)
                                       gCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAg                                                  <  1:2308778/93‑1 (MQ=255)
                                         aaCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGc                                                >  1:4536219/1‑93 (MQ=255)
                                         aaCGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGc                                                >  2:1201456/1‑93 (MQ=255)
                                           cGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCa                                              >  2:2905619/1‑93 (MQ=255)
                                           cGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCa                                              >  1:4168910/1‑93 (MQ=255)
                                            gTTTTGCAGCGCGGGTTCGAGGGCAGTGCCTGCATTACCCGCAGCAGCAAAAACAGTCTCACCCAGCGGCGGCTGGGCCGCTTTCCAGGCCAg                                             <  2:3734047/93‑1 (MQ=255)
                                             ttttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgc                                            <  2:2005476/93‑1 (MQ=255)
                                             ttttGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAgc                                            >  1:2445282/1‑93 (MQ=255)
                                                 gCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGcgcgg                                        >  2:297389/1‑92 (MQ=255)
                                                   aGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGc                                      >  2:3268698/1‑93 (MQ=255)
                                                   aGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGc                                      <  1:3514688/93‑1 (MQ=255)
                                                    gcgcGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCTCTTTCCAGGCCAGCGCGTGCt                                     >  2:4494022/1‑93 (MQ=255)
                                                    gcgcGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCt                                     >  2:3674325/1‑93 (MQ=255)
                                                    gcgcGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCt                                     <  1:4295228/93‑1 (MQ=255)
                                                    gcgcGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCt                                     <  1:2635747/93‑1 (MQ=255)
                                                       cgGGTTCCAGTGCAGTGCCTGCCTTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCACGCCAGCGCGTGCTcgc                                  >  2:1793583/1‑93 (MQ=255)
                                                       cgGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTcgc                                  <  1:3659571/93‑1 (MQ=255)
                                                        gggTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTcgcg                                 >  1:751536/1‑93 (MQ=255)
                                                          gTTCCAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTACTCGcgcc                               >  2:1740284/1‑93 (MQ=255)
                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            <  2:2315518/93‑1 (MQ=255)
                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            >  2:1547771/1‑93 (MQ=255)
                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            <  1:3265452/93‑1 (MQ=255)
                                                             ccAGTGCAGTGCCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGACGCTTTCCAGGCCAGCGCGTGCTCGcgcccgc                            >  1:3122567/1‑93 (MQ=255)
                                                               agtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCccgccg                          >  2:284585/1‑93 (MQ=255)
                                                                gtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         >  1:2922370/1‑93 (MQ=255)
                                                                gtgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGt                         >  1:2119133/1‑93 (MQ=255)
                                                                 tgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGtt                        >  2:1498500/1‑93 (MQ=255)
                                                                 tgcagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGtt                        >  1:1883994/1‑93 (MQ=255)
                                                                   cagtgcCTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTAc                      <  2:3797594/93‑1 (MQ=255)
                                                                    agtgcCTGCATTCCCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTAcc                     <  1:1685060/93‑1 (MQ=255)
                                                                      tgcCTGCATTCCCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCaa                   <  2:2299805/93‑1 (MQ=255)
                                                                        ccTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATc                 >  1:71663/1‑93 (MQ=255)
                                                                        ccTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATc                 >  2:3844477/1‑93 (MQ=255)
                                                                        ccTGCAGTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGGGCTCGCGCCCGCCGTTACCAATc                 <  2:1344091/93‑1 (MQ=255)
                                                                         cTGCATTACCCGGAGCAGCAAAAACGGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGTCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCa                <  1:154976/93‑1 (MQ=255)
                                                                         cTGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCa                >  2:3910648/1‑93 (MQ=255)
                                                                          tGCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCAc               >  2:930406/1‑93 (MQ=255)
                                                                           gCATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACt              >  1:607280/1‑93 (MQ=255)
                                                                            cATTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTa             >  2:299577/1‑93 (MQ=255)
                                                                             aTTACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTaa            >  1:3690212/1‑93 (MQ=255)
                                                                              ttACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAAt           <  1:3833516/93‑1 (MQ=255)
                                                                               tACCCGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATa          >  2:4507894/1‑93 (MQ=255)
                                                                                ccccGGAGCAGCAAAAACAGTCTCAACCAGCGGCGCCTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATAc         <  2:4560762/92‑1 (MQ=255)
                                                                                  ccGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACtt       <  2:3988462/93‑1 (MQ=255)
                                                                                  ccGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACtt       >  1:445926/1‑93 (MQ=255)
                                                                                  ccGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACtt       >  1:1770667/1‑93 (MQ=255)
                                                                                   cGGAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACttt      <  2:2592858/93‑1 (MQ=255)
                                                                                     gAGCAGCAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCa    >  1:559386/1‑93 (MQ=255)
                                                                                      agcagcAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCac   >  1:3780298/1‑92 (MQ=255)
                                                                                      agcagcAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCAt   <  1:1481494/93‑1 (MQ=255)
                                                                                       gcagcaAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATc  >  1:2910821/1‑93 (MQ=255)
                                                                                       gcagcaAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATc  >  1:2834833/1‑93 (MQ=255)
                                                                                       gcagcaAAAACAGTCTCAACCAGCGCCGACTGCCCCGCTTTCCAGCCCAGCCCGTGCTCCCGCCCGCCGTTACCAATCACTAATACTTTCATc  <  2:4482837/93‑1 (MQ=255)
                                                                                       gCAAAAAAAACAGTCTAAACCAGCGGCGACTGGCCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATc  <  2:4327905/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGAAATCCAACAGCGCCGGGATATCGGTCACGCCAATAGCAACGTTTTGCAGCGCGGGTTCCAGTGCAGTGCCTGCATTACCCGGAGCAACAAAAACAGTCTCAACCAGCGGCGACTGGGCCGCTTTCCAGGCCAGCGCGTGCTCGCGCCCGCCGTTACCAATCACTAATACTTTCATC  >  NC_000913/4203331‑4203510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑