BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,214,346	A→G	E430E (GAA→GAG)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,214,346	0	A	G	100.0%	771.6 / NA	243	E430E (GAA→GAG)	aceB	malate synthase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (111/132); total (111/132)

Only 100 of 254 total aligned reads displayed.

TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC  >  NC_000913/4214256‑4214434

 aCTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCg                                                                                       <  2:3978474/93‑1 (MQ=255)

 aCTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCg                                                                                       >  1:3401203/1‑93 (MQ=255)

    gCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTgg                                                                                    <  2:14560/93‑1 (MQ=255)

    gCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTgg                                                                                    >  2:2643112/1‑93 (MQ=255)

    gCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTgg                                                                                    <  1:2293451/93‑1 (MQ=255)

      cGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAt                                                                                  <  2:1300356/93‑1 (MQ=255)

      cGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAt                                                                                  >  1:2564607/1‑93 (MQ=255)

         tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                               >  2:2379697/1‑93 (MQ=255)

         tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                               >  2:2409267/1‑93 (MQ=255)

          cAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATctct                                                                              <  1:146097/93‑1 (MQ=255)

          cAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATctct                                                                              <  1:1563533/93‑1 (MQ=255)

            gctgctGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTgg                                                                            >  2:3607011/1‑93 (MQ=255)

            gctgctGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTgg                                                                            >  1:113587/1‑93 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                          >  2:2279773/1‑93 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                          <  1:2795700/93‑1 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                          >  1:1043031/1‑93 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGGGGATCTCTGGCa                                                                          >  1:3289646/1‑93 (MQ=255)

               gctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCaa                                                                         <  2:1139205/93‑1 (MQ=255)

               gctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCaa                                                                         <  2:3530489/93‑1 (MQ=255)

                ctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAAc                                                                        <  1:4327540/93‑1 (MQ=255)

                  ggCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACgg                                                                      >  1:1185412/1‑93 (MQ=255)

                   gCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGc                                                                     <  2:4370486/93‑1 (MQ=255)

                     aCCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtg                                                                   >  2:3676676/1‑93 (MQ=255)

                     aCCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtg                                                                   >  1:1269902/1‑93 (MQ=255)

                      ccTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgt                                                                  >  1:2433712/1‑93 (MQ=255)

                      ccTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgt                                                                  >  1:2157936/1‑93 (MQ=255)

                       cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                 >  2:2743474/1‑93 (MQ=255)

                       cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                 >  1:3453209/1‑93 (MQ=255)

                        ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgagt                                                                >  2:1755398/1‑93 (MQ=255)

                         tgtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgtg                                                               >  1:1313441/1‑93 (MQ=255)

                             aTGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGa                                                           <  1:2096677/93‑1 (MQ=255)

                              tGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAt                                                          >  2:3317176/1‑93 (MQ=255)

                                gTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAttt                                                        <  1:235787/93‑1 (MQ=255)

                                 tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                       <  2:2615932/93‑1 (MQ=255)

                                  gAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAt                                                      <  1:3577598/93‑1 (MQ=255)

                                   aaCGCACCGAAGAAGGTATGCCCTCCAACATTCGCGGGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATg                                                     <  2:501029/93‑1 (MQ=255)

                                      gCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGcc                                                  <  1:2974115/93‑1 (MQ=255)

                                       cACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCt                                                 >  2:1474811/1‑93 (MQ=255)

                                         ccGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGa                                               >  2:4307341/1‑93 (MQ=255)

                                          cGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGAt                                              <  1:2693525/93‑1 (MQ=255)

                                           gaagaaGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATg                                             <  1:3824110/93‑1 (MQ=255)

                                           gaagaaGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATg                                             >  2:1783866/1‑93 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            <  1:3741318/93‑1 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            >  1:3691743/1‑93 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            >  2:1048545/1‑93 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            <  1:2406369/93‑1 (MQ=255)

                                             agaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGa                                           >  1:3092337/1‑93 (MQ=255)

                                              gaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGaa                                          >  2:3806123/1‑93 (MQ=255)

                                              gaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGaa                                          >  1:760741/1‑93 (MQ=255)

                                                agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGa                                        >  2:2136986/1‑93 (MQ=255)

                                                agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGa                                        <  2:2855924/93‑1 (MQ=255)

                                                agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGa                                        >  2:1212488/1‑93 (MQ=255)

                                                 ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                       >  1:574250/1‑93 (MQ=255)

                                                 ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                       <  1:4071572/93‑1 (MQ=255)

                                                 ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                       >  2:4393080/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGGGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:1658004/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:4163654/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  1:271015/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:3272172/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:759505/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATGCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     <  2:2300692/93‑1 (MQ=255)

                                                    aTGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcg                                    <  1:170451/93‑1 (MQ=255)

                                                      gcgcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcggc                                  <  2:2125208/93‑1 (MQ=255)

                                                      gcgcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcggc                                  <  1:3937378/93‑1 (MQ=255)

                                                        gcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGa                                >  1:854634/1‑93 (MQ=255)

                                                        gcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGa                                >  2:3011479/1‑93 (MQ=255)

                                                         cgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                               <  2:4101550/93‑1 (MQ=255)

                                                         cgcCAACATGCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                               <  2:3443474/93‑1 (MQ=255)

                                                           ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGAGGCGACgg                             <  2:4558259/93‑1 (MQ=255)

                                                            cAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGc                            <  1:4592055/93‑1 (MQ=255)

                                                            cAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGc                            <  2:2574345/93‑1 (MQ=255)

                                                             aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                           >  1:3380630/1‑93 (MQ=255)

                                                              aCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTg                          >  1:4053358/1‑93 (MQ=255)

                                                                 ttCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGaaa                       >  1:2150794/1‑93 (MQ=255)

                                                                 ttCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGaaa                       >  1:2851681/1‑93 (MQ=255)

                                                                    gcgTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAttt                    >  1:192706/1‑93 (MQ=255)

                                                                     cgTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTc                   <  1:2114756/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  1:2556242/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  2:2466388/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  2:3135823/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  1:178008/93‑1 (MQ=255)

                                                                       tGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTccc                 <  2:2097081/93‑1 (MQ=255)

                                                                        ggCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCg                >  1:1016071/1‑93 (MQ=255)

                                                                        ggCTGCGCAGTACATCGAGACGTGAATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCg                <  1:4664899/93‑1 (MQ=255)

                                                                            gtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAcc            <  2:2887640/93‑1 (MQ=255)

                                                                              gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc          <  1:722103/93‑1 (MQ=255)

                                                                                 gTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGAt       >  1:1515024/1‑93 (MQ=255)

                                                                                 gTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCCTACCTCGAt       >  2:107196/1‑93 (MQ=255)

                                                                                  tACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATc      >  2:1865152/1‑93 (MQ=255)

                                                                                  tACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATc      <  1:1623065/93‑1 (MQ=255)

                                                                                  tACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATc      >  2:3225710/1‑93 (MQ=255)

                                                                                   tcATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt     <  1:4460791/92‑1 (MQ=255)

                                                                                   tcATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTCATGGCCTGCTGGAAGCTGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt     <  2:676061/92‑1 (MQ=255)

                                                                                   aCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt     <  2:2012574/93‑1 (MQ=255)

                                                                                    cATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTg    >  1:88563/1‑93 (MQ=255)

                                                                                    cATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTg    <  2:4591508/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  <  2:559497/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  <  1:4303303/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  <  1:3867501/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  >  2:961545/1‑93 (MQ=255)

TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC  >  NC_000913/4214256‑4214434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`