BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,052	A→G	E122G (GAG→GGG)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,052	0	A	G	100.0%	680.0 / NA	216	E122G (GAG→GGG)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (110/106); total (110/106)

Only 100 of 224 total aligned reads displayed.

TGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCC  >  NC_000913/4214964‑4215141

tGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCgg                                                                                       >  2:2725510/1‑93 (MQ=255)

tGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGAAAACTCGGTGCCAGCTGTGGTGGGGccg                                                                                       >  2:685176/1‑91 (MQ=255)

tGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCCGATCAGTCGCTCTATCCGGCAAACTAGGTGCCAGCTGTGGTGGGGCgg                                                                                       >  2:271685/1‑93 (MQ=255)

 ggCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGa                                                                                      <  2:644733/93‑1 (MQ=255)

 ggCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGa                                                                                      <  2:4172966/93‑1 (MQ=255)

  gCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGAt                                                                                     >  2:3175970/1‑93 (MQ=255)

  gCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGAt                                                                                     >  1:1861379/1‑93 (MQ=255)

   cAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATc                                                                                    <  1:3815753/93‑1 (MQ=255)

    aGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATca                                                                                   >  1:2569114/1‑93 (MQ=255)

     ggTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATcaa                                                                                  >  2:1960845/1‑93 (MQ=255)

       tAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATcaaca                                                                                >  2:3390012/1‑93 (MQ=255)

        aGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATcaacaa                                                                               <  2:3728483/93‑1 (MQ=255)

        aGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATcaacaa                                                                               <  1:622784/93‑1 (MQ=255)

        aGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATcaacaa                                                                               <  2:4642031/93‑1 (MQ=255)

         gcggcgGACGCTACCCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCaacaac                                                                              <  1:4144836/93‑1 (MQ=255)

           ggcggACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAacac                                                                            <  1:2345906/93‑1 (MQ=255)

             cggACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCt                                                                          <  2:2678382/93‑1 (MQ=255)

             cggACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCt                                                                          >  1:3974735/1‑93 (MQ=255)

              ggACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCtt                                                                         <  2:3881183/93‑1 (MQ=255)

              ggACGCTAACCTGGCGGCCAGCATGGATCCGGATCAGTCGCTCTATCCGGCAAACTCGGGGCCAGCTGTGGTGGGGGGGATCAACAACACCtt                                                                         >  2:4390607/1‑93 (MQ=255)

                 cGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCcg                                                                      <  2:181231/93‑1 (MQ=255)

                  gCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCcgt                                                                     >  1:1792490/1‑93 (MQ=255)

                   cTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCcgtc                                                                    <  1:911090/93‑1 (MQ=255)

                    tAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCcgtcg                                                                   <  1:1260543/93‑1 (MQ=255)

                      aCCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTg                                                                 >  1:2097712/1‑93 (MQ=255)

                      aCCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTg                                                                 >  2:4229923/1‑93 (MQ=255)

                       ccTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGc                                                                >  1:468575/1‑93 (MQ=255)

                        cTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGTCGGATCAACAACACCTTCCGTCGTGcc                                                               >  1:2867141/1‑93 (MQ=255)

                        cTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGcc                                                               >  2:110543/1‑93 (MQ=255)

                        cTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGcc                                                               <  2:2387144/93‑1 (MQ=255)

                         tGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCg                                                              <  2:3297206/93‑1 (MQ=255)

                            cggcCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATc                                                           <  1:1194457/93‑1 (MQ=255)

                            cggcCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATc                                                           <  2:1226761/93‑1 (MQ=255)

                             ggcCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACGCCTTCCGTCGTGCCGATCa                                                          <  2:4575136/93‑1 (MQ=255)

                             ggcCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCa                                                          <  1:756322/93‑1 (MQ=255)

                             ggcCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCa                                                          >  2:3264/1‑93 (MQ=255)

                             ggcCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCa                                                          <  1:1460194/93‑1 (MQ=255)

                              gcCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAg                                                         >  1:1241989/1‑93 (MQ=255)

                               ccAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGa                                                        <  2:1200992/93‑1 (MQ=255)

                                cAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGAt                                                       <  1:3307605/93‑1 (MQ=255)

                                cAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGAt                                                       <  1:3056510/93‑1 (MQ=255)

                                 aGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATc                                                      >  1:3838433/1‑93 (MQ=255)

                                 aGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATc                                                      >  1:3210172/1‑93 (MQ=255)

                                  gCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATcc                                                     >  1:3400027/1‑93 (MQ=255)

                                  gCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATcc                                                     >  1:1421590/1‑93 (MQ=255)

                                     tGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAAt                                                  >  2:882447/1‑93 (MQ=255)

                                     tGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAAt                                                  >  1:1598688/1‑93 (MQ=255)

                                     tGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAAt                                                  >  1:599178/1‑93 (MQ=255)

                                      gTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATg                                                 >  2:2576391/1‑93 (MQ=255)

                                       tATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATgg                                                >  1:3375813/1‑93 (MQ=255)

                                        aTCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGt                                               <  2:208387/93‑1 (MQ=255)

                                         tCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTc                                              <  1:310744/93‑1 (MQ=255)

                                         tCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTc                                              >  1:2926918/1‑93 (MQ=255)

                                          ccGGATCAGTTGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTcc                                             <  2:334259/93‑1 (MQ=255)

                                          ccGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTcc                                             <  1:4552541/93‑1 (MQ=255)

                                          ccGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTcc                                             <  2:3024807/93‑1 (MQ=255)

                                           cGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCcg                                            <  1:1390506/93‑1 (MQ=255)

                                           cGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCcg                                            <  1:2009146/93‑1 (MQ=255)

                                            ggATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCcgc                                           >  1:3574646/1‑93 (MQ=255)

                                             gATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCcgcg                                          <  2:3993705/93‑1 (MQ=255)

                                               tCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCggg                                        >  1:23554/1‑93 (MQ=255)

                                               tCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCggg                                        >  2:2420502/1‑93 (MQ=255)

                                                  gTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCAt                                     >  1:2464790/1‑93 (MQ=255)

                                                  gTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCAt                                     >  1:977154/1‑93 (MQ=255)

                                                    cGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTg                                   >  2:4071854/1‑93 (MQ=255)

                                                    cGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTg                                   >  2:763378/1‑93 (MQ=255)

                                                     gCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGa                                  >  2:3930344/1‑93 (MQ=255)

                                                     gCTCTATCCGGCAAACTCGGTCCCAGATCAGGTGGGGCGGATCATCAAGACCTTCCATCGTGCCGATCAGATCCAATGGTCCGCGGGCATTgt                                  >  2:2213842/1‑92 (MQ=255)

                                                      ctctATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAg                                 <  2:3668330/93‑1 (MQ=255)

                                                      ctctATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAg                                 <  1:2972048/93‑1 (MQ=255)

                                                       tctATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGc                                <  1:3823801/93‑1 (MQ=255)

                                                       tctATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGc                                >  2:1681755/1‑93 (MQ=255)

                                                         tATCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCg                              >  1:399165/1‑93 (MQ=255)

                                                          aTCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCgg                             <  2:2448490/93‑1 (MQ=255)

                                                          aTCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCgg                             >  1:2291676/1‑93 (MQ=255)

                                                           tCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCggg                            >  1:3432169/1‑93 (MQ=255)

                                                           tCCGGCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCggg                            >  2:2013849/1‑93 (MQ=255)

                                                            ccGGCAAGCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGc                           <  1:2623419/93‑1 (MQ=255)

                                                              ggCAAACTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGa                         >  1:2540151/1‑93 (MQ=255)

                                                                 aaaCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATcc                      >  2:3304757/1‑93 (MQ=255)

                                                                 aaaCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATcc                      >  2:3035297/1‑93 (MQ=255)

                                                                  aaCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATctg                     >  1:409095/1‑91 (MQ=255)

                                                                  aaCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCcg                     <  2:4160149/93‑1 (MQ=255)

                                                                   aCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCcgc                    <  1:4403281/93‑1 (MQ=255)

                                                                   aCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCcgc                    >  1:2824829/1‑93 (MQ=255)

                                                                   aCTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCcgc                    <  1:3151391/93‑1 (MQ=255)

                                                                    cTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTTAGACGGGCGATAcgcg                   >  2:3334225/1‑93 (MQ=255)

                                                                    cTCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCcgcg                   >  1:3703414/1‑93 (MQ=255)

                                                                     tCGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCgcgc                  >  1:691746/1‑93 (MQ=255)

                                                                     tCGGTGCCAGCTGTGGGGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATTGCCCGCGGGCATTGAGCCGGGGGATTCtcgc                  >  2:531624/1‑93 (MQ=255)

                                                                      cGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCt                 <  2:3746824/93‑1 (MQ=255)

                                                                      cGGTGCCAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCt                 >  1:1660116/1‑93 (MQ=255)

                                                                           ccAGCTGTGGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTc            <  2:1453478/93‑1 (MQ=255)

                                                                                tgtgGTGGGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTa       >  1:1690178/1‑93 (MQ=255)

                                                                                  tggtggGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACt     >  2:4452014/1‑93 (MQ=255)

                                                                                   ggtggGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACtt    >  2:1507266/1‑93 (MQ=255)

                                                                                    gtggGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTc   <  1:1964214/93‑1 (MQ=255)

                                                                                    gtggGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTc   <  2:2043660/93‑1 (MQ=255)

                                                                                     tggGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTcc  >  2:1072574/1‑93 (MQ=255)

                                                                                     tggGGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTcc  <  2:3485643/93‑1 (MQ=255)

TGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCC  >  NC_000913/4214964‑4215141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`