BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	52,554	A→G	L288P (CTG→CCG)	pdxA ←	PdxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	52,554	0	A	G	100.0%	556.5 / NA	181	L288P (CTG→CCG)	pdxA	PdxA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (85/96); total (85/96)

Only 100 of 194 total aligned reads displayed.

AGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCAGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCG  >  NC_000913/52464‑52643

 gcgcCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAgggcgggc                                                                                        >  2:1935690/1‑93 (MQ=255)

   gcCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGccc                                                                                      >  2:935913/1‑93 (MQ=255)

   gcCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGccc                                                                                      >  2:1998026/1‑93 (MQ=255)

     cGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAg                                                                                    <  1:1657157/93‑1 (MQ=255)

      gTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGc                                                                                   <  2:981059/93‑1 (MQ=255)

      gTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGc                                                                                   >  1:810756/1‑93 (MQ=255)

        aataaAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGt                                                                                 <  1:42465/93‑1 (MQ=255)

        aataaAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGt                                                                                 >  2:4199525/1‑93 (MQ=255)

         ataaAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTa                                                                                >  1:2089336/1‑93 (MQ=255)

         ataaAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTa                                                                                >  1:3778919/1‑93 (MQ=255)

          taaAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGtca                                                                               >  2:1082759/1‑91 (MQ=255)

          taaAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTaa                                                                               <  2:1718438/93‑1 (MQ=255)

           aaaaCTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAAt                                                                              <  2:1868269/93‑1 (MQ=255)

            aaccTGCCGACATCGGCTTTGCCACGGCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATg                                                                             <  2:3748767/90‑1 (MQ=255)

            aaaCTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATg                                                                             >  1:2233351/1‑93 (MQ=255)

               cTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTc                                                                          >  2:698845/1‑93 (MQ=255)

                tGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTca                                                                         >  2:2804826/1‑93 (MQ=255)

                tGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTca                                                                         >  2:3564985/1‑93 (MQ=255)

                   cGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCacac                                                                      >  1:2646957/1‑93 (MQ=255)

                    gACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACAcc                                                                     <  2:4249637/93‑1 (MQ=255)

                    gACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACAcc                                                                     >  2:1555206/1‑93 (MQ=255)

                    gACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAAGGTTCACAcc                                                                     >  1:1257716/1‑93 (MQ=255)

                     aCATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACcg                                                                    <  1:1063653/93‑1 (MQ=255)

                      cATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACcgc                                                                   <  2:2055218/93‑1 (MQ=255)

                      cATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACcgc                                                                   <  2:3967313/93‑1 (MQ=255)

                        tCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCgcgc                                                                 >  1:2804372/1‑93 (MQ=255)

                          ggCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGccc                                                               >  1:2259432/1‑93 (MQ=255)

                           gCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCg                                                              >  1:2414812/1‑93 (MQ=255)

                           gCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGACCg                                                              >  2:2205894/1‑93 (MQ=255)

                            cTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGa                                                             >  1:1671838/1‑93 (MQ=255)

                             tttGCCGCGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGaa                                                            <  1:1507953/93‑1 (MQ=255)

                             tttGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGaa                                                            <  2:1507162/93‑1 (MQ=255)

                             tttGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGaa                                                            <  2:4286584/93‑1 (MQ=255)

                             tttGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGaa                                                            >  1:1298604/1‑93 (MQ=255)

                              ttGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAg                                                           >  2:2469941/1‑93 (MQ=255)

                              ttGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAg                                                           >  1:973755/1‑93 (MQ=255)

                                   ccGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTg                                                      <  2:106856/92‑1 (MQ=255)

                                   aggccccGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTg                                                      <  2:1185755/89‑1 (MQ=255)

                                   aCGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTg                                                      >  2:1320619/1‑93 (MQ=255)

                                   aCGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTg                                                      >  2:1726346/1‑93 (MQ=255)

                                   aCGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTg                                                      <  2:3109398/93‑1 (MQ=255)

                                    cGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTgg                                                     <  1:718058/93‑1 (MQ=255)

                                     gTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGt                                                    <  1:4659401/93‑1 (MQ=255)

                                      tCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGTCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTa                                                   <  2:3307762/93‑1 (MQ=255)

                                        ccGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTAtt                                                 <  1:4062946/93‑1 (MQ=255)

                                        ccGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGCCGGGCCCAGCGTAATGTTCACACCGCGCCCGACGCCCTGGTAtt                                                 >  2:1160662/1‑93 (MQ=255)

                                           ccAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTa                                              >  2:2861400/1‑93 (MQ=255)

                                           ccAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTa                                              >  1:1061602/1‑93 (MQ=255)

                                             aGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGc                                            >  2:2542384/1‑93 (MQ=255)

                                               ttCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCAc                                          >  2:859881/1‑93 (MQ=255)

                                               gtCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAAGAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCAc                                          <  2:3475843/92‑1 (MQ=255)

                                                tCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACg                                         <  1:2044992/93‑1 (MQ=255)

                                                 cAAGCGCGGGTCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACCCCGCGCCCGAAGCCCTGGTATTTTAGCACgg                                        <  1:3212880/93‑1 (MQ=255)

                                                    gcgcGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGaa                                     <  1:3357991/93‑1 (MQ=255)

                                                      gcgGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGa                                   <  2:1992776/93‑1 (MQ=255)

                                                      gcgGTGCCGTGGTCCACTGATGTGCGAATAAAGAGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGa                                   >  1:4570348/1‑93 (MQ=255)

                                                         gTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGGCCGAAGCCCTGGTATTTTAGCACGGGACGAccc                                >  1:665015/1‑93 (MQ=255)

                                                         gTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGAccc                                <  2:236393/93‑1 (MQ=255)

                                                         gTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGAccc                                <  1:3508883/93‑1 (MQ=255)

                                                         gTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGAccc                                >  1:4337055/1‑93 (MQ=255)

                                                         gTGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGAccc                                <  1:3065983/93‑1 (MQ=255)

                                                          tGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCt                               <  1:2440057/93‑1 (MQ=255)

                                                          tGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCt                               <  2:1298476/93‑1 (MQ=255)

                                                          tGCCGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCt                               <  2:3169924/93‑1 (MQ=255)

                                                            ccGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGa                             <  1:1238535/93‑1 (MQ=255)

                                                            ccGTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGa                             <  2:266486/93‑1 (MQ=255)

                                                             cGTGGTCCACTGATGTGCGAATAAAGGTCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGAt                            <  1:3779102/93‑1 (MQ=255)

                                                              gttgTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATc                           <  1:939457/90‑1 (MQ=255)

                                                              gTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATc                           >  1:1617934/1‑93 (MQ=255)

                                                              gTGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATc                           <  2:2433606/93‑1 (MQ=255)

                                                               tGGTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCg                          >  1:3100597/1‑93 (MQ=255)

                                                                ggTCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGt                         <  1:3726214/93‑1 (MQ=255)

                                                                  tCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTgg                       <  1:217365/93‑1 (MQ=255)

                                                                  tCCACTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTgg                       <  2:2937339/93‑1 (MQ=255)

                                                                    cccTGATGTGCGACAAAAGGGCCGGCCCCGCCTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGCCCCTGATCGTGGTa                     <  2:4649496/91‑1 (MQ=255)

                                                                     aCTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTAc                    >  1:3394504/1‑93 (MQ=255)

                                                                     aCTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTAc                    >  1:893879/1‑93 (MQ=255)

                                                                      cTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACa                   <  1:3975726/93‑1 (MQ=255)

                                                                      cTGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACa                   <  2:366832/93‑1 (MQ=255)

                                                                       tGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACAt                  <  1:1568372/93‑1 (MQ=255)

                                                                       tGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACAt                  >  1:1161227/1‑93 (MQ=255)

                                                                       tGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACAt                  <  1:468287/93‑1 (MQ=255)

                                                                       tGATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACAt                  <  1:3846076/93‑1 (MQ=255)

                                                                        gATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATc                 >  2:3410343/1‑93 (MQ=255)

                                                                        gATGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATc                 <  2:44743/93‑1 (MQ=255)

                                                                         aTGTGCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCg                >  2:727296/1‑93 (MQ=255)

                                                                          tgtgCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGc               <  1:3451978/93‑1 (MQ=255)

                                                                           gtgCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGcc              <  1:4359904/93‑1 (MQ=255)

                                                                             gCGAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAg            <  1:1447483/93‑1 (MQ=255)

                                                                               gAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCa          >  1:2743175/1‑93 (MQ=255)

                                                                               gAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCa          >  1:2915792/1‑93 (MQ=255)

                                                                               gAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCa          >  1:2468264/1‑93 (MQ=255)

                                                                               gAATAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCa          <  2:14139/93‑1 (MQ=255)

                                                                                gaTAAAGGTCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCAc         <  2:1893087/92‑1 (MQ=255)

                                                                                aaTAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCAc         >  2:3813662/1‑93 (MQ=255)

                                                                                aaTAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCAc         <  2:1372780/93‑1 (MQ=255)

                                                                                  tAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCAcgg       >  1:1522896/1‑93 (MQ=255)

                                                                                  tAAAGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCAcgg       <  1:688109/93‑1 (MQ=255)

                                                                                     aGGGCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACAGCGCCAGCAcggcgt    <  2:3686774/93‑1 (MQ=255)

                                                                                       ggCGGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCAcggcgtcg  <  2:3614972/93‑1 (MQ=255)

AGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGCGAATAAAGGGCAGGCCCAGCGTAATGTTCACACCGCGCCCGAAGCCCTGGTATTTTAGCACGGGAAGACCCTGATCGTGGTACATCGCCAGCACGGCGTCG  >  NC_000913/52464‑52643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`