BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,242,957	C→T	E348K (GAA→AAA)	malE ←	periplasmic maltose‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,242,957	0	C	T	100.0%	953.8 / NA	261	E348K (GAA→AAA)	malE	periplasmic maltose‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (116/145); total (116/145)

Only 100 of 267 total aligned reads displayed.

TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046

tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         <  2:1514111/93‑1 (MQ=255)

tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         <  1:3170105/93‑1 (MQ=255)

tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         >  2:1928702/1‑93 (MQ=255)

 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  2:473572/93‑1 (MQ=255)

 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  1:4308469/93‑1 (MQ=255)

 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  2:1028818/93‑1 (MQ=255)

  gcggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtg                                                                                       <  1:3785893/93‑1 (MQ=255)

    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  1:840115/93‑1 (MQ=255)

     gcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggtg                                                                                    >  2:3690563/1‑93 (MQ=255)

     gcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTCTTTCAtggtg                                                                                    <  2:1205360/93‑1 (MQ=255)

      cgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggtgg                                                                                   <  2:2201788/93‑1 (MQ=255)

       gTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggc                                                                                  >  1:138799/1‑93 (MQ=255)

        ttGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggcg                                                                                 >  1:1759706/1‑93 (MQ=255)

          gATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggcggc                                                                               <  1:4402992/93‑1 (MQ=255)

           aTCACCGCAGTACGCACGGCATACCGGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCa                                                                              <  1:4466768/93‑1 (MQ=255)

           aTCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCa                                                                              >  1:2688916/1‑93 (MQ=255)

            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             <  2:6316/93‑1 (MQ=255)

            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             <  1:2456222/93‑1 (MQ=255)

             cACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAAt                                                                            <  2:3062730/93‑1 (MQ=255)

              aCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATa                                                                           <  1:3452380/93‑1 (MQ=255)

              aCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATa                                                                           <  2:4037700/93‑1 (MQ=255)

               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:2314019/93‑1 (MQ=255)

               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:2170684/93‑1 (MQ=255)

               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:3293018/93‑1 (MQ=255)

                    gtgcGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     <  1:737341/90‑1 (MQ=255)

                      aCGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATc                                                                   <  1:2405860/93‑1 (MQ=255)

                       cGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCt                                                                  <  2:4337741/93‑1 (MQ=255)

                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 >  2:4385356/1‑93 (MQ=255)

                            ggCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGc                                                             <  1:3729597/93‑1 (MQ=255)

                             gCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGcc                                                            >  1:4054433/1‑93 (MQ=255)

                              cATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCa                                                           <  1:2500070/93‑1 (MQ=255)

                              cATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCa                                                           >  1:1799209/1‑93 (MQ=255)

                               aTACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCac                                                          >  1:616134/1‑92 (MQ=255)

                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAc                                                         >  2:2111533/1‑93 (MQ=255)

                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATATTTCGCCAAc                                                         >  2:4219578/1‑93 (MQ=255)

                                  cgaGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAActc                                                       <  1:4658085/91‑1 (MQ=255)

                                  ccAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAActc                                                       >  1:4191830/1‑93 (MQ=255)

                                    aGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGGGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                     >  2:3864814/1‑93 (MQ=255)

                                    aGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                     >  1:903295/1‑93 (MQ=255)

                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  1:3546313/1‑93 (MQ=255)

                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  1:1960538/1‑93 (MQ=255)

                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  2:2958742/1‑93 (MQ=255)

                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  1:1896559/93‑1 (MQ=255)

                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGAAATACGTGGATCTTTCGCCAACTCTTcc                                                   >  1:4614538/1‑93 (MQ=255)

                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCGCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  1:3769809/93‑1 (MQ=255)

                                            gACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaa                                             <  1:2768216/93‑1 (MQ=255)

                                             aCATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaaa                                            >  1:1049329/1‑92 (MQ=255)

                                               aTCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGAc                                          <  2:3626057/93‑1 (MQ=255)

                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         >  1:1474077/1‑93 (MQ=255)

                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  2:3112779/93‑1 (MQ=255)

                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  2:204944/93‑1 (MQ=255)

                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       >  1:1305243/1‑93 (MQ=255)

                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       >  2:3932241/1‑93 (MQ=255)

                                                  tGCGGGATGTTCGGAATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  2:872912/93‑1 (MQ=255)

                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  1:2430192/93‑1 (MQ=255)

                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  1:283003/93‑1 (MQ=255)

                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  1:4592350/93‑1 (MQ=255)

                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     <  2:3281195/93‑1 (MQ=255)

                                                     gggATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgc                                    <  1:338137/93‑1 (MQ=255)

                                                     gggATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgc                                    <  1:2233826/93‑1 (MQ=255)

                                                      ggATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgcg                                   >  1:3981259/1‑93 (MQ=255)

                                                        aTGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAAGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCt                                 <  1:285374/93‑1 (MQ=255)

                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               >  2:2635660/1‑93 (MQ=255)

                                                           ttCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACg                              >  2:1959617/1‑93 (MQ=255)

                                                               gCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAc                          >  1:2345648/1‑93 (MQ=255)

                                                                cATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAcc                         <  2:4344477/93‑1 (MQ=255)

                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:2132204/1‑93 (MQ=255)

                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:3615944/1‑93 (MQ=255)

                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:496928/1‑93 (MQ=255)

                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  2:1266232/1‑93 (MQ=255)

                                                                   gATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAg                      >  1:1682073/1‑93 (MQ=255)

                                                                    aTTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGc                     >  2:7405/1‑93 (MQ=255)

                                                                    aTTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGc                     >  2:3094035/1‑93 (MQ=255)

                                                                    aTTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGc                     >  1:570544/1‑93 (MQ=255)

                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  1:984079/93‑1 (MQ=255)

                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  >  1:2945385/1‑93 (MQ=255)

                                                                        cACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGtt                 <  1:1008911/93‑1 (MQ=255)

                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                >  2:1724850/1‑93 (MQ=255)

                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGgtt                >  2:2445048/1‑93 (MQ=255)

                                                                          ccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTg               <  1:2597040/93‑1 (MQ=255)

                                                                           cTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGt              <  1:189582/93‑1 (MQ=255)

                                                                            tttCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc             >  2:2918311/1‑93 (MQ=255)

                                                                            tttCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc             >  2:1606203/1‑93 (MQ=255)

                                                                               ttGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCttt          <  1:3307910/92‑1 (MQ=255)

                                                                               ctaggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCttt          >  2:1864980/4‑93 (MQ=255)

                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         <  1:452974/93‑1 (MQ=255)

                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         >  1:1165013/1‑93 (MQ=255)

                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         <  2:2748244/93‑1 (MQ=255)

                                                                                 tggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttat        <  1:2097359/92‑1 (MQ=255)

                                                                                 gggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttat        >  1:2899696/1‑93 (MQ=255)

                                                                                  tgCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  2:1170918/2‑93 (MQ=255)

                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  2:1371419/1‑93 (MQ=255)

                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  2:4493072/93‑1 (MQ=255)

                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  2:3421404/93‑1 (MQ=255)

                                                                                   gCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatta      >  2:4109639/1‑93 (MQ=255)

                                                                                   gCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatta      >  2:109999/1‑93 (MQ=255)

                                                                                     gTTTTTCATGGTGGCGGCATTACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  2:4551804/1‑93 (MQ=255)

                                                                                     gTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  2:2828968/1‑93 (MQ=255)

                                                                                     gTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  1:3927886/1‑93 (MQ=255)

                                                                                      ttttTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc   <  2:674438/93‑1 (MQ=255)

TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`