BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,243,337	A→G	L221P (CTG→CCG)	malE ←	periplasmic maltose‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,243,337	0	A	G	100.0%	725.6 / NA	228	L221P (CTG→CCG)	malE	periplasmic maltose‑binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (116/112); total (116/112)

Only 100 of 238 total aligned reads displayed.

TCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCC  >  NC_000913/4243248‑4243426

tCATCGCTGTTTCGCCTTTTTTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGGTTTTTTTTAATCAGGTCAACCAGGa                                                                                        >  2:2311154/1‑93 (MQ=255)

 cATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGaa                                                                                       <  1:4449710/93‑1 (MQ=255)

 cATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGaa                                                                                       <  1:4573532/93‑1 (MQ=255)

 aaTCGCTGTTTCGCCTTTATTAAATGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAAACGGGaa                                                                                       <  2:1114538/92‑1 (MQ=255)

  aTCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAg                                                                                      >  2:3346530/1‑93 (MQ=255)

   tCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAgg                                                                                     <  2:529288/93‑1 (MQ=255)

   tCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTCTTAATCAGGTCAACCGGGAAgg                                                                                     >  1:3785721/1‑93 (MQ=255)

     gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   <  1:1872379/93‑1 (MQ=255)

     gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   >  1:2703108/1‑93 (MQ=255)

     gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   <  1:2996684/93‑1 (MQ=255)

     gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   <  2:2849775/93‑1 (MQ=255)

     gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   <  1:3565400/93‑1 (MQ=255)

     gCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTc                                                                                   >  2:3988790/1‑93 (MQ=255)

      cTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCa                                                                                  <  1:4178908/93‑1 (MQ=255)

       tGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAg                                                                                 >  1:1809576/1‑93 (MQ=255)

        gTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGa                                                                                <  1:1592631/93‑1 (MQ=255)

        gTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGa                                                                                <  2:4054433/93‑1 (MQ=255)

            cGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCg                                                                            >  2:864761/1‑93 (MQ=255)

             gCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGc                                                                           >  1:2362014/1‑93 (MQ=255)

                tttATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCttt                                                                        >  1:2561191/1‑93 (MQ=255)

                  tattaAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTcg                                                                      <  2:1330858/93‑1 (MQ=255)

                   attaAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTcgc                                                                     <  2:2692432/93‑1 (MQ=255)

                      aaaGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGcc                                                                  <  1:2987243/93‑1 (MQ=255)

                      aaaGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGcc                                                                  >  2:1745340/1‑93 (MQ=255)

                        aGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACACGCTTTCGCGCCAg                                                                >  2:2642436/1‑93 (MQ=255)

                         ggCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGc                                                               >  1:5956/1‑93 (MQ=255)

                             gCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGttt                                                           >  2:3146319/1‑92 (MQ=255)

                             gCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTa                                                           >  1:1031325/1‑93 (MQ=255)

                               ttCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATc                                                         >  1:819776/1‑93 (MQ=255)

                                 cTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCa                                                       <  1:1958002/93‑1 (MQ=255)

                                 cTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCa                                                       <  1:583058/93‑1 (MQ=255)

                                  tGCTATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCAc                                                      <  1:2399465/93‑1 (MQ=255)

                                    ctaTGGAATAATCGGTGTCTGCATTCATTGGTTTTTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCTAGCTTTTTCCACGc                                                    <  2:4614538/91‑1 (MQ=255)

                                     gATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGcc                                                   >  2:1778279/1‑93 (MQ=255)

                                      aTGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGccc                                                  >  2:1577142/1‑93 (MQ=255)

                                      aTGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGccc                                                  >  1:290693/1‑93 (MQ=255)

                                      aTGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGccc                                                  >  1:950775/1‑93 (MQ=255)

                                      aTGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGccc                                                  <  1:3094311/93‑1 (MQ=255)

                                      aTGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGccc                                                  >  1:2523135/1‑93 (MQ=255)

                                       tGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCa                                                 <  2:701796/93‑1 (MQ=255)

                                        ggAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCAc                                                >  1:1240821/1‑93 (MQ=255)

                                           gTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACGCGCTTTCGCTCCAGCGTTATCCTCGCCCActgt                                             >  2:1722925/1‑90 (MQ=255)

                                           gTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTc                                             <  1:1098358/93‑1 (MQ=255)

                                           gTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTc                                             >  1:276792/1‑93 (MQ=255)

                                            tAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCt                                            >  2:3751594/1‑93 (MQ=255)

                                              aTCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCttt                                          >  2:397382/1‑93 (MQ=255)

                                              aTCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCttt                                          <  1:4127893/93‑1 (MQ=255)

                                                cGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCGCACGTCTTTaa                                        <  2:2919575/93‑1 (MQ=255)

                                                cGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTaa                                        <  1:79169/93‑1 (MQ=255)

                                                cGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTaa                                        <  1:1363638/93‑1 (MQ=255)

                                                cGGTGTCGGCATTGATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCACCCCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTaa                                        <  2:2801373/93‑1 (MQ=255)

                                                  gtgtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATg                                      >  2:3378798/1‑93 (MQ=255)

                                                   tgtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGt                                     <  1:1693959/93‑1 (MQ=255)

                                                   tgtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGt                                     >  1:2929664/1‑93 (MQ=255)

                                                    gtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTc                                    >  1:3390810/1‑93 (MQ=255)

                                                    gtCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTc                                    >  1:4255775/1‑93 (MQ=255)

                                                     tCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCg                                   >  1:4194264/1‑93 (MQ=255)

                                                     tCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCg                                   >  1:1283822/1‑93 (MQ=255)

                                                       tGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTa                                 <  2:1315000/93‑1 (MQ=255)

                                                       tGCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTa                                 >  1:1743905/1‑93 (MQ=255)

                                                        gCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTAc                                <  1:42017/93‑1 (MQ=255)

                                                        gCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTAc                                >  1:1457960/1‑93 (MQ=255)

                                                        gCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTAc                                <  2:4249290/93‑1 (MQ=255)

                                                        gCATTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGCTATCCACGCCCACGTCTTTAATGTCGTat                                >  1:541277/1‑92 (MQ=255)

                                                          aTTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACtt                              >  2:3520695/1‑93 (MQ=255)

                                                          aTTCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACtt                              >  2:671285/1‑93 (MQ=255)

                                                           ttCATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTg                             >  2:2454130/1‑93 (MQ=255)

                                                             cATGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGcc                           >  2:3689146/1‑93 (MQ=255)

                                                              aTGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCg                          >  1:4002159/1‑93 (MQ=255)

                                                              aTGTGTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCg                          >  1:3282519/1‑93 (MQ=255)

                                                               tgtgTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGt                         >  2:2552504/1‑93 (MQ=255)

                                                               tgtgTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGt                         >  1:2662132/1‑93 (MQ=255)

                                                               tgtgTTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACCTGCCGt                         >  2:2997418/1‑93 (MQ=255)

                                                                gtgtTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtt                        >  2:2481280/1‑93 (MQ=255)

                                                                gtgtTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtt                        <  2:1994729/93‑1 (MQ=255)

                                                                gtgtTTGTTTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtt                        <  2:4167599/93‑1 (MQ=255)

                                                                 tgtttgttTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGttt                       <  1:2565477/93‑1 (MQ=255)

                                                                 tgtttgttTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGttt                       >  2:3829675/1‑93 (MQ=255)

                                                                 tgtttgttTTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGttt                       >  2:742908/1‑93 (MQ=255)

                                                                  gtttgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtttt                      <  1:3723619/93‑1 (MQ=255)

                                                                  gtttgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGtttt                      >  1:3593005/1‑93 (MQ=255)

                                                                   tttgtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTc                     >  1:1144219/1‑93 (MQ=255)

                                                                      gtttTTAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATa                  <  2:1037331/93‑1 (MQ=255)

                                                                       tttttAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATAc                 <  2:896903/93‑1 (MQ=255)

                                                                         tttAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACtt               >  2:3458577/1‑93 (MQ=255)

                                                                           tAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGa             <  2:561384/93‑1 (MQ=255)

                                                                           tAATCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGa             <  2:2159283/93‑1 (MQ=255)

                                                                            aaTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCATCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGaa            <  1:1535710/93‑1 (MQ=255)

                                                                            aaTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGaa            <  2:439111/93‑1 (MQ=255)

                                                                            aaTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGaa            >  2:4682745/1‑93 (MQ=255)

                                                                            aaTCAGGGCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGaa            >  2:4479152/1‑93 (MQ=255)

                                                                             aTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAAc           <  1:2081468/93‑1 (MQ=255)

                                                                             aTCAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAAc           >  2:2737856/1‑93 (MQ=255)

                                                                               cAGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGc         <  1:2888559/93‑1 (MQ=255)

                                                                                aGGTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCa        <  1:2180003/93‑1 (MQ=255)

                                                                                  gTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATa      >  2:4316147/1‑93 (MQ=255)

                                                                                  gTCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATa      <  2:1299269/93‑1 (MQ=255)

                                                                                   tCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATaa     <  2:4272581/93‑1 (MQ=255)

                                                                                   tCAACCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATaa     <  2:1678909/93‑1 (MQ=255)

                                                                                      aCCGGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAAccc  <  1:3482583/93‑1 (MQ=255)

TCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCC  >  NC_000913/4243248‑4243426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`