BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,041	A→G	S863P (TCC→CCC)	acrB ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,041	0	A	G	99.4%	499.4 / NA	159	S863P (TCC→CCC)	acrB	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (78/80); minor base C (0/1); total (78/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCG  >  NC_000913/480953‑481130

tACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATc                                                                                       >  2:1470619/1‑93 (MQ=255)

  cAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATccc                                                                                     >  1:4378370/1‑93 (MQ=255)

   aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                    <  2:3912409/93‑1 (MQ=255)

   aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                    <  2:393304/93‑1 (MQ=255)

   aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                    >  2:1170282/1‑93 (MQ=255)

    gcgcCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCg                                                                                   >  1:3171430/1‑93 (MQ=255)

       ccgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTcc                                                                                <  2:2809334/93‑1 (MQ=255)

        cgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCa                                                                               >  2:716452/1‑93 (MQ=255)

         gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAg                                                                              >  2:348104/1‑93 (MQ=255)

          ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGt                                                                             <  1:1189098/93‑1 (MQ=255)

          ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGt                                                                             >  1:4558938/1‑93 (MQ=255)

            aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCa                                                                           <  1:914213/93‑1 (MQ=255)

             gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCAt                                                                          >  1:1597690/1‑93 (MQ=255)

             gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCAt                                                                          >  2:520495/1‑93 (MQ=255)

              acacAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATa                                                                         <  1:4060766/93‑1 (MQ=255)

              acacAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATa                                                                         <  1:1398543/93‑1 (MQ=255)

               cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAg                                                                        <  1:1631144/93‑1 (MQ=255)

               cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAg                                                                        <  2:1128738/93‑1 (MQ=255)

                acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGc                                                                       <  1:4552477/93‑1 (MQ=255)

                  aGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCa                                                                     <  2:2040520/93‑1 (MQ=255)

                  aGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCa                                                                     >  1:3620759/1‑93 (MQ=255)

                  aGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCa                                                                     >  2:1855286/1‑93 (MQ=255)

                   ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCaa                                                                    >  2:3486523/1‑93 (MQ=255)

                     aaCACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAaca                                                                  <  2:450154/93‑1 (MQ=255)

                      acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAacac                                                                 <  2:1880353/93‑1 (MQ=255)

                       cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACAcc                                                                >  1:4196017/1‑93 (MQ=255)

                       cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACAcc                                                                <  1:3805393/93‑1 (MQ=255)

                        acGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCg                                                               >  2:2036993/1‑93 (MQ=255)

                          gACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGt                                                             >  1:40251/1‑93 (MQ=255)

                          gACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGt                                                             >  2:1653360/1‑93 (MQ=255)

                           aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                            <  1:1543676/93‑1 (MQ=255)

                            cAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                           >  2:2005856/1‑93 (MQ=255)

                               tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCa                                                        <  2:2257541/93‑1 (MQ=255)

                                cAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAg                                                       >  1:211393/1‑93 (MQ=255)

                                cAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAg                                                       <  2:3416135/93‑1 (MQ=255)

                                 aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                      >  1:1810999/1‑93 (MQ=255)

                                 aaCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                      <  1:2238652/93‑1 (MQ=255)

                                  aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                     <  1:945324/93‑1 (MQ=255)

                                  aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                     >  1:1653867/1‑93 (MQ=255)

                                  aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                     <  1:3486482/93‑1 (MQ=255)

                                     aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                  <  2:472406/93‑1 (MQ=255)

                                     aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                  >  1:2318524/1‑93 (MQ=255)

                                     aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                  >  2:4462143/1‑93 (MQ=255)

                                      aaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCGGTTTGCt                                                 <  2:2618223/93‑1 (MQ=255)

                                      aaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                 >  2:1246671/1‑93 (MQ=255)

                                      aaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                 <  2:1337110/93‑1 (MQ=255)

                                      aaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                 >  2:2918102/1‑93 (MQ=255)

                                       aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                                >  1:858443/1‑93 (MQ=255)

                                        tCGCGTACCGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCg                                               >  2:4550714/1‑93 (MQ=255)

                                        tCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCg                                               >  1:1659499/1‑93 (MQ=255)

                                         cgcgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGc                                              <  1:3144680/93‑1 (MQ=255)

                                           cgTACAGTGAGGGTGCCGGGTGGCGGGAAGTACGTCCCTAATGGGCCACCCCCTCCCGGTCAT‑GCCAACACCGGTAGGCAGTTTGCTCGCCAg                                           <  2:1457597/93‑1 (MQ=255)

                                           cgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCa                                            <  1:3280468/93‑1 (MQ=255)

                                             tACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgt                                          <  2:241095/93‑1 (MQ=255)

                                             tACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgt                                          <  1:3023427/93‑1 (MQ=255)

                                              aCAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgtt                                         <  1:3395115/93‑1 (MQ=255)

                                                   gAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTcc                                    >  1:2347966/1‑93 (MQ=255)

                                                   gAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTcc                                    <  1:1314314/93‑1 (MQ=255)

                                                   gAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTcc                                    >  2:573701/1‑93 (MQ=255)

                                                    aaGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                   <  1:2960876/93‑1 (MQ=255)

                                                     aGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAt                                  <  1:1109240/93‑1 (MQ=255)

                                                     aGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAt                                  >  1:1392325/1‑93 (MQ=255)

                                                      ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 <  2:810453/93‑1 (MQ=255)

                                                      ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 <  1:1548885/93‑1 (MQ=255)

                                                      ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 <  1:1574804/93‑1 (MQ=255)

                                                      ggTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                 <  2:4492237/93‑1 (MQ=255)

                                                        tGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAg                               >  2:1249929/1‑93 (MQ=255)

                                                        tGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAg                               <  2:103046/93‑1 (MQ=255)

                                                        ggCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAg                               <  1:3288774/92‑1 (MQ=255)

                                                         gCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGc                              >  1:2586126/1‑93 (MQ=255)

                                                         gCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGc                              <  2:3120436/93‑1 (MQ=255)

                                                          ccTGGTTGCCGGAGAGACGTTCCTGATAGGGGATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAgat                             >  2:1753890/1‑91 (MQ=255)

                                                          ccTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCt                             >  2:3725365/1‑93 (MQ=255)

                                                          ccTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCt                             >  2:1943592/1‑93 (MQ=255)

                                                           cTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                            >  2:3727668/1‑93 (MQ=255)

                                                            tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCCGTTGTTCCATCAGCttc                           >  2:3740925/1‑91 (MQ=255)

                                                            tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                           >  2:2848749/1‑93 (MQ=255)

                                                            tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                           >  1:3149436/1‑93 (MQ=255)

                                                            tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                           >  2:3530081/1‑93 (MQ=255)

                                                             ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                          >  1:3640113/1‑93 (MQ=255)

                                                              gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                         <  1:1960714/93‑1 (MQ=255)

                                                              gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                         <  1:955277/93‑1 (MQ=255)

                                                              gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                         <  2:2351314/93‑1 (MQ=255)

                                                                   cGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCtt                    >  1:151017/1‑93 (MQ=255)

                                                                     gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                  <  1:625665/93‑1 (MQ=255)

                                                                        agaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCg               >  2:4445602/1‑93 (MQ=255)

                                                                         gaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCgg              >  1:1245111/1‑93 (MQ=255)

                                                                         gaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCgg              >  1:4055848/1‑93 (MQ=255)

                                                                         gaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCgg              >  1:4509421/1‑93 (MQ=255)

                                                                           cGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa            <  1:1026375/93‑1 (MQ=255)

                                                                           cGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa            <  1:3818506/93‑1 (MQ=255)

                                                                           cGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa            <  1:3019308/93‑1 (MQ=255)

                                                                            gTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTAc           >  2:686652/1‑93 (MQ=255)

                                                                             ttCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACt          >  1:2814841/1‑93 (MQ=255)

                                                                             ttCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACt          >  1:283797/1‑93 (MQ=255)

                                                                              tCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtt         <  2:3025065/93‑1 (MQ=255)

                                                                               ccTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACttt        <  2:3058512/93‑1 (MQ=255)

                                                                                 tGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTa      >  1:824048/1‑93 (MQ=255)

                                                                                    tAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAccc   <  2:4418389/93‑1 (MQ=255)

                                                                                     aGGGCATCCCCGTCCAGTCATAGCAAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCg  <  2:2989351/93‑1 (MQ=255)

TACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCG  >  NC_000913/480953‑481130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`