BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,491	A→G	V101A (GTA→GCA)	yjcH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,491	0	A	G	99.6%	839.0 / ‑5.5	263	V101A (GTA→GCA)	yjcH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (124/138); minor base C (0/1); total (124/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 269 total aligned reads displayed.

CTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC  >  NC_000913/4282401‑4282580

ctctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGcc                                                                                         >  2:1587675/1‑93 (MQ=255)

ctctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGcc                                                                                         >  1:255932/1‑93 (MQ=255)

   tACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCa                                                                                      >  1:1514529/1‑93 (MQ=255)

    aCGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCAt                                                                                     <  1:540852/93‑1 (MQ=255)

      ggCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGc                                                                                   <  1:2459082/93‑1 (MQ=255)

          ccGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGa                                                                               <  1:3721160/93‑1 (MQ=255)

           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              >  1:3065477/1‑93 (MQ=255)

           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              >  1:3671605/1‑93 (MQ=255)

           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              <  2:2242163/93‑1 (MQ=255)

           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              >  1:3898495/1‑93 (MQ=255)

            gCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACt                                                                             >  2:2865558/1‑93 (MQ=255)

              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  2:2546464/1‑93 (MQ=255)

              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  1:2871019/1‑93 (MQ=255)

              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  2:332555/1‑93 (MQ=255)

              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           <  1:4202144/93‑1 (MQ=255)

              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           <  2:2342177/93‑1 (MQ=255)

              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  1:1315930/1‑93 (MQ=255)

                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         >  1:1261932/1‑93 (MQ=255)

                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         <  1:1080207/93‑1 (MQ=255)

                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         >  1:1033061/1‑93 (MQ=255)

                 tAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTCCCTCATGCAGGACTTCatt                                                                        <  2:4524772/93‑1 (MQ=255)

                  aGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCatta                                                                       <  1:1731782/93‑1 (MQ=255)

                  aGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCatta                                                                       <  2:1005146/93‑1 (MQ=255)

                     aTCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCAttatta                                                                    <  1:3473277/93‑1 (MQ=255)

                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:2724391/93‑1 (MQ=255)

                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:3553812/93‑1 (MQ=255)

                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:2136808/93‑1 (MQ=255)

                        cgcgGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGa                                                                 <  1:1862394/93‑1 (MQ=255)

                         gcggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGAc                                                                <  1:72521/93‑1 (MQ=255)

                          cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACg                                                               <  1:2259663/93‑1 (MQ=255)

                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              <  1:1117449/93‑1 (MQ=255)

                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              >  1:3800703/1‑93 (MQ=255)

                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              >  1:220042/1‑93 (MQ=255)

                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              <  2:2089351/93‑1 (MQ=255)

                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             <  1:1655135/93‑1 (MQ=255)

                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             <  2:1280866/93‑1 (MQ=255)

                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             >  2:3797878/1‑93 (MQ=255)

                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             <  2:2963793/93‑1 (MQ=255)

                              gTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCg                                                           >  1:910862/1‑93 (MQ=255)

                               ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGa                                                          >  1:1000947/1‑93 (MQ=255)

                               ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGa                                                          >  1:297219/1‑93 (MQ=255)

                               ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGa                                                          <  1:3683023/93‑1 (MQ=255)

                                tAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGaa                                                         <  1:2872537/93‑1 (MQ=255)

                                tAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGaa                                                         <  2:725885/93‑1 (MQ=255)

                                 aGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAt                                                        <  2:3173155/93‑1 (MQ=255)

                                  gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                                                       <  2:2054495/93‑1 (MQ=255)

                                  gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                                                       >  1:3256328/1‑93 (MQ=255)

                                   cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                                                      <  1:1578722/93‑1 (MQ=255)

                                    tGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                                                     <  2:1329173/93‑1 (MQ=255)

                                     gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGc                                                    >  1:1631914/1‑93 (MQ=255)

                                     gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGc                                                    >  2:1643821/1‑93 (MQ=255)

                                      cGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGcc                                                   <  1:2039592/93‑1 (MQ=255)

                                        aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGt                                                 <  1:3583694/93‑1 (MQ=255)

                                        aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGt                                                 >  1:2713869/1‑93 (MQ=255)

                                          aGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc                                               >  2:403104/1‑93 (MQ=255)

                                          aGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc                                               >  2:29981/1‑93 (MQ=255)

                                           gggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcg                                              >  2:3747984/1‑93 (MQ=255)

                                                    gcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGa                                     <  2:4639276/93‑1 (MQ=255)

                                                    gcggcAAGCGCCGACAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGa                                     <  2:614070/93‑1 (MQ=255)

                                                       gcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGt                                  <  1:633786/93‑1 (MQ=255)

                                                         aaGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAg                                >  2:3889853/1‑93 (MQ=255)

                                                         aaGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAg                                >  1:4504971/1‑93 (MQ=255)

                                                          aGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGa                               <  1:2753204/93‑1 (MQ=255)

                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              <  2:502790/93‑1 (MQ=255)

                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              >  2:474455/1‑93 (MQ=255)

                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              <  1:329533/93‑1 (MQ=255)

                                                            cgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATa                             <  1:4434809/93‑1 (MQ=255)

                                                               cGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCg                          >  1:3277092/1‑93 (MQ=255)

                                                                   aGAACTTTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      >  1:4082193/1‑93 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCGTCATGCAGTACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  2:2621320/93‑1 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  2:1146790/93‑1 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      >  2:1025472/1‑93 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCAGTTCGCCCGCCAGATGTAGATACCGGTga                      >  2:3202759/1‑93 (MQ=255)

                                                                    gAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTgag                     >  2:4180777/1‑93 (MQ=255)

                                                                      aCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGaa                   >  1:4669281/1‑93 (MQ=255)

                                                                       ctctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAc                  <  2:794338/93‑1 (MQ=255)

                                                                        tctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACa                 >  1:3412399/1‑93 (MQ=255)

                                                                         ctTTGCATAATGCTTGTGCCTCATGCAGGACTTCATTATTAATACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaa                <  2:4324764/93‑1 (MQ=255)

                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               <  2:3216706/93‑1 (MQ=255)

                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               >  1:610862/1‑93 (MQ=255)

                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               <  1:1224923/93‑1 (MQ=255)

                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               >  2:3697997/1‑93 (MQ=255)

                                                                           tttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg              <  1:4139014/93‑1 (MQ=255)

                                                                             tCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGga            <  1:4299890/93‑1 (MQ=255)

                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTTGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  2:1173035/1‑93 (MQ=255)

                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  2:3514311/1‑93 (MQ=255)

                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  1:1976054/1‑93 (MQ=255)

                                                                                tgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt         <  1:735451/93‑1 (MQ=255)

                                                                                tgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt         >  2:585693/1‑93 (MQ=255)

                                                                                tgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt         <  2:1504451/93‑1 (MQ=255)

                                                                                 gatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATc        <  1:1437553/93‑1 (MQ=255)

                                                                                   tgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      >  2:3976214/1‑93 (MQ=255)

                                                                                   tgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      >  1:195017/1‑93 (MQ=255)

                                                                                   tgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      >  2:929870/1‑93 (MQ=255)

                                                                                    gCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcaca     <  1:1781261/93‑1 (MQ=255)

                                                                                     cTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACaa    >  2:3272958/1‑93 (MQ=255)

                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   <  1:2659269/93‑1 (MQ=255)

                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   >  1:4395893/1‑93 (MQ=255)

                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   >  2:2265692/1‑93 (MQ=255)

                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   >  2:1979881/1‑93 (MQ=255)

CTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC  >  NC_000913/4282401‑4282580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`