BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	486,435	A→G	T226A (ACC→GCC)	aefA →	putative alpha helix protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	486,435	0	A	G	100.0%	504.3 / NA	161	T226A (ACC→GCC)	aefA	putative alpha helix protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (80/81); total (80/81)

Only 100 of 166 total aligned reads displayed.

TACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAA  >  NC_000913/486346‑486524

tACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCg                                                                                        >  1:4606944/1‑93 (MQ=255)

tACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCg                                                                                        <  1:4659030/93‑1 (MQ=255)

 aCGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGt                                                                                       <  2:476291/93‑1 (MQ=255)

 aCGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGACGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGt                                                                                       <  2:1350639/93‑1 (MQ=255)

  cGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTc                                                                                      <  2:810304/93‑1 (MQ=255)

  cGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTc                                                                                      >  2:1897910/1‑93 (MQ=255)

   gTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCt                                                                                     <  2:3178894/93‑1 (MQ=255)

   gTCCCAGCAAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCt                                                                                     <  2:3120098/93‑1 (MQ=255)

    tCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGCACGCCGTCtt                                                                                    >  2:462101/1‑93 (MQ=255)

    tCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCtt                                                                                    <  1:1377248/93‑1 (MQ=255)

    tCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCtt                                                                                    <  2:4054501/93‑1 (MQ=255)

    tCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCtt                                                                                    >  1:2386162/1‑93 (MQ=255)

     cccAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTg                                                                                   >  2:3309184/1‑93 (MQ=255)

      ccagccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGc                                                                                  <  1:360647/93‑1 (MQ=255)

       cagccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCa                                                                                 <  2:3463055/93‑1 (MQ=255)

        agccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAg                                                                                <  2:3077010/93‑1 (MQ=255)

         gccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAgg                                                                               >  2:1210928/1‑93 (MQ=255)

          ccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGa                                                                              <  2:4118883/93‑1 (MQ=255)

          ccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGa                                                                              <  1:3472912/93‑1 (MQ=255)

           cagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGAt                                                                             <  1:3073754/93‑1 (MQ=255)

           cagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGAt                                                                             >  2:205666/1‑93 (MQ=255)

            agAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATa                                                                            <  2:737604/93‑1 (MQ=255)

            agAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATa                                                                            >  1:878491/1‑93 (MQ=255)

            agAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATa                                                                            <  2:1767491/93‑1 (MQ=255)

            agAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATa                                                                            >  2:2460703/1‑93 (MQ=255)

             gAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATAc                                                                           >  2:2823218/1‑93 (MQ=255)

             gAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATAc                                                                           >  1:2534954/1‑93 (MQ=255)

              aaaGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATAcc                                                                          >  1:1810636/1‑93 (MQ=255)

               aaGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCt                                                                         <  2:3572013/93‑1 (MQ=255)

               aaGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCt                                                                         <  2:543097/93‑1 (MQ=255)

                aGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCtt                                                                        >  1:1509091/1‑93 (MQ=255)

                  tgtTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGc                                                                      >  2:252888/1‑93 (MQ=255)

                  tgtTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGc                                                                      <  1:3418230/93‑1 (MQ=255)

                  tgtTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACAGTCTTGCAGGATACCTTGc                                                                      >  2:933276/1‑93 (MQ=255)

                   gtTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCa                                                                     >  1:2795841/1‑93 (MQ=255)

                    ttAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATAACTTGCaa                                                                    >  2:1119886/1‑93 (MQ=255)

                     tAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCaaa                                                                   <  2:2610174/93‑1 (MQ=255)

                      aaTGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCaaaa                                                                  <  2:2641043/93‑1 (MQ=255)

                      aaTGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCATGCaaaa                                                                  <  2:4454146/93‑1 (MQ=255)

                        tGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGc                                                                <  2:371749/93‑1 (MQ=255)

                        tGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGc                                                                >  2:2721321/1‑93 (MQ=255)

                          cAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCaa                                                              <  2:668110/93‑1 (MQ=255)

                          cAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCaa                                                              <  1:1863422/93‑1 (MQ=255)

                           aGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAAc                                                             <  1:1962665/93‑1 (MQ=255)

                           aGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAAc                                                             <  1:2889073/93‑1 (MQ=255)

                            ggCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACg                                                            <  1:1031858/93‑1 (MQ=255)

                             gCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGt                                                           <  1:4345765/93‑1 (MQ=255)

                             gCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGt                                                           <  2:2934247/93‑1 (MQ=255)

                               ccAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTgt                                                         >  1:541488/1‑92 (MQ=255)

                                 agcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGAtt                                                       <  2:2249507/93‑1 (MQ=255)

                                 agcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGAtt                                                       >  2:1376421/1‑93 (MQ=255)

                                  gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTa                                                      >  2:300210/1‑93 (MQ=255)

                                  gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTa                                                      >  2:4659495/1‑93 (MQ=255)

                                  gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTa                                                      >  2:2007748/1‑93 (MQ=255)

                                   cagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTAc                                                     <  1:629301/93‑1 (MQ=255)

                                    agGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACg                                                    >  1:2489504/1‑93 (MQ=255)

                                     ggCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGt                                                   >  1:2991797/1‑93 (MQ=255)

                                     ggCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGt                                                   >  2:2670677/1‑93 (MQ=255)

                                        gTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGAc                                                <  1:1451915/93‑1 (MQ=255)

                                         ttGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACg                                               >  2:81211/1‑93 (MQ=255)

                                         ttGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACg                                               >  1:4554767/1‑93 (MQ=255)

                                             tGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGa                                           >  1:1946983/1‑93 (MQ=255)

                                             tGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGa                                           >  2:1713182/1‑93 (MQ=255)

                                             tGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGa                                           >  2:3040333/1‑93 (MQ=255)

                                              gAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGaa                                          >  2:2149757/1‑93 (MQ=255)

                                                aTGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACa                                        <  1:72603/93‑1 (MQ=255)

                                                  gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAgc                                      <  1:61366/93‑1 (MQ=255)

                                                    ggAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAgcgc                                    <  1:2475562/93‑1 (MQ=255)

                                                    ggAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAgcgc                                    >  2:4279906/1‑93 (MQ=255)

                                                     gagaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCt                                   <  2:2315289/93‑1 (MQ=255)

                                                      agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTc                                  <  1:3247937/93‑1 (MQ=255)

                                                        aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGt                                >  2:3989261/1‑93 (MQ=255)

                                                        aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGt                                >  1:3061686/1‑93 (MQ=255)

                                                         ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTc                               <  1:4076110/93‑1 (MQ=255)

                                                         ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTc                               >  1:336213/1‑93 (MQ=255)

                                                          tGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCt                              >  2:2599807/1‑93 (MQ=255)

                                                           gACCAGCAGCGTAAAAGCCTGGAAGTGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTg                             <  2:220224/93‑1 (MQ=255)

                                                             ccAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGa                           <  2:3234264/93‑1 (MQ=255)

                                                             ccAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGa                           <  1:2648269/93‑1 (MQ=255)

                                                             ccAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGa                           <  2:4012007/93‑1 (MQ=255)

                                                              cagcagCGTAAAAGCCTGGAAGGGAACGCCGGCTTGCAGGATACCTTGCAAAAGCAACGTGATTAAGGGACGGCGAACCGCGCTCGTCTGGac                          >  2:2283298/1‑92 (MQ=255)

                                                                gcagcGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCa                        <  1:3326992/93‑1 (MQ=255)

                                                                  agcGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCAcc                      >  1:4452084/1‑93 (MQ=255)

                                                                  agcGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCAcc                      <  2:1780347/93‑1 (MQ=255)

                                                                   gcGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCa                     <  2:102372/93‑1 (MQ=255)

                                                                     gTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTGCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGt                   <  1:3880412/93‑1 (MQ=255)

                                                                           gCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAAc             >  2:4209001/1‑93 (MQ=255)

                                                                           gCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAAc             <  1:3654336/93‑1 (MQ=255)

                                                                           gCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAAc             >  2:64799/1‑93 (MQ=255)

                                                                            ccTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACt            >  1:1118455/1‑93 (MQ=255)

                                                                              tGGAAGTGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGt          <  1:3666665/93‑1 (MQ=255)

                                                                              tGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGt          >  2:3572998/1‑93 (MQ=255)

                                                                              tGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGt          <  1:1754271/93‑1 (MQ=255)

                                                                              tGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGt          >  2:1284747/1‑93 (MQ=255)

                                                                               ggAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGtt         <  2:1319028/93‑1 (MQ=255)

                                                                                gAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTg        >  2:1846538/1‑93 (MQ=255)

                                                                                  aGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCa      >  2:4448390/1‑93 (MQ=255)

                                                                                   gagAACGCCGTCTTGCAGGATCGCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTGCAACTGTTGCaa     <  1:2829500/91‑1 (MQ=255)

                                                                                     gAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaaga   >  1:2629541/1‑93 (MQ=255)

                                                                                      aaCGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGcaacaa  <  2:1855087/93‑1 (MQ=255)

TACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAA  >  NC_000913/486346‑486524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`