BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,441,383	T→C	S475S (AGT→AGC)	ytfM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,441,383	0	T	C	100.0%	545.9 / NA	172	S475S (AGT→AGC)	ytfM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (70/102); total (70/102)

Only 100 of 178 total aligned reads displayed.

TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472

tGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAt                                                                                         <  2:759286/93‑1 (MQ=255)

tGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAt                                                                                         <  2:1494080/93‑1 (MQ=255)

tGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAt                                                                                         >  1:4635965/1‑93 (MQ=255)

 gTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAtt                                                                                        >  2:178149/1‑93 (MQ=255)

 gTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAtt                                                                                        <  2:1000281/93‑1 (MQ=255)

    acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACGCCCGGACCGCCGTTTCTCGGCCGGGGGCGACCGCAGCATTctg                                                                                     >  2:2641873/1‑91 (MQ=255)

     cacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTg                                                                                    >  1:2426319/1‑93 (MQ=255)

      acGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTgg                                                                                   <  1:4551111/93‑1 (MQ=255)

       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGc                                                                                  <  2:3043551/93‑1 (MQ=255)

       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGc                                                                                  <  1:569318/93‑1 (MQ=255)

       cgcgGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGc                                                                                  >  2:4144583/1‑93 (MQ=255)

          ggCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtac                                                                               <  2:4671010/93‑1 (MQ=255)

            cACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaa                                                                             <  1:4176789/93‑1 (MQ=255)

             aCGCTGGGCTGGATTGACACCGGTGAATTCGACACCGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCACAGCATTGGTGGCtaaaaa                                                                            <  2:2325413/93‑1 (MQ=255)

             aCGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaa                                                                            >  2:400052/1‑93 (MQ=255)

             aCGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaa                                                                            <  1:4034924/93‑1 (MQ=255)

                 tgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaataca                                                                        >  2:1187137/1‑93 (MQ=255)

                    gctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTacaaatacaaat                                                                     >  2:1201099/1‑93 (MQ=255)

                     ctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATc                                                                    <  1:1321721/93‑1 (MQ=255)

                     ctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATc                                                                    <  1:1228408/93‑1 (MQ=255)

                      tggATTGAATCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCt                                                                   <  2:3319208/93‑1 (MQ=255)

                       ggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTa                                                                  <  2:2464258/93‑1 (MQ=255)

                       ggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTa                                                                  <  2:3710194/93‑1 (MQ=255)

                       ggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTa                                                                  <  1:2789113/93‑1 (MQ=255)

                         aTTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATc                                                                >  1:2758093/1‑93 (MQ=255)

                         aTTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATc                                                                <  1:3080252/93‑1 (MQ=255)

                            gAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCt                                                             <  1:2167353/93‑1 (MQ=255)

                             aaaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTc                                                            >  2:3829409/1‑93 (MQ=255)

                             aaaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTc                                                            >  1:4376346/1‑93 (MQ=255)

                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                           <  2:4023936/93‑1 (MQ=255)

                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                           >  1:1918327/1‑93 (MQ=255)

                              aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                           <  1:1317947/93‑1 (MQ=255)

                                ccGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGTCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGa                                                         >  2:3446931/1‑93 (MQ=255)

                                  ggTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGaaa                                                       <  1:3515494/93‑1 (MQ=255)

                                   gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                      >  2:4137867/1‑93 (MQ=255)

                                   gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                      <  2:2940233/93‑1 (MQ=255)

                                   gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                      >  1:1925433/1‑93 (MQ=255)

                                   gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                      <  2:3738760/93‑1 (MQ=255)

                                   gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                      >  2:212643/1‑93 (MQ=255)

                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     >  2:1304386/1‑93 (MQ=255)

                                    tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                     <  1:3409571/93‑1 (MQ=255)

                                     gATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAc                                                    <  1:2994184/93‑1 (MQ=255)

                                        ttCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGcc                                                 <  1:271183/93‑1 (MQ=255)

                                         tCGACAAAGTACCGCCGGATCCGCGTTTCTTCGGCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCa                                                <  1:4027390/93‑1 (MQ=255)

                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:3601651/93‑1 (MQ=255)

                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  1:2924859/93‑1 (MQ=255)

                                           gACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                              <  2:1026022/93‑1 (MQ=255)

                                            aCAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACg                                             >  1:475994/1‑93 (MQ=255)

                                             cAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                            <  1:248743/93‑1 (MQ=255)

                                             cAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                            <  2:3970605/93‑1 (MQ=255)

                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACggg                                           >  1:759381/1‑92 (MQ=255)

                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           <  2:1055251/93‑1 (MQ=255)

                                              aaaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGt                                           >  1:1472311/1‑93 (MQ=255)

                                               aaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTg                                          <  1:799823/93‑1 (MQ=255)

                                                 gTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAc                                        >  2:3120749/1‑93 (MQ=255)

                                                   aCCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCt                                      >  2:4636338/1‑93 (MQ=255)

                                                   aCCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCt                                      <  2:3118034/93‑1 (MQ=255)

                                                   aCCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCt                                      <  2:2239332/93‑1 (MQ=255)

                                                    ccgcggGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTg                                     <  1:2597465/93‑1 (MQ=255)

                                                    ccgccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTg                                     <  2:2359531/93‑1 (MQ=255)

                                                    ccgccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTg                                     <  2:317725/93‑1 (MQ=255)

                                                      gccgGATCTGCGTTTCTTCGCCGGGGGCTACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaa                                   <  1:1292842/93‑1 (MQ=255)

                                                       ccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                  >  1:1061573/1‑93 (MQ=255)

                                                        cgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                 >  1:908886/1‑93 (MQ=255)

                                                        cgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                 >  1:439814/1‑93 (MQ=255)

                                                         ggATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                >  2:3327617/1‑93 (MQ=255)

                                                         ggATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                <  2:2487160/93‑1 (MQ=255)

                                                         ggATCTGCGTTTCTTCGCAGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                                <  1:408699/93‑1 (MQ=255)

                                                          gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  2:2986441/1‑93 (MQ=255)

                                                          gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               <  1:1187137/93‑1 (MQ=255)

                                                          gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                               >  2:1079062/1‑93 (MQ=255)

                                                            tCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                             >  1:3732400/1‑93 (MQ=255)

                                                             cTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGcc                            >  2:4409718/1‑93 (MQ=255)

                                                               gCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                          <  1:1849870/93‑1 (MQ=255)

                                                               gCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                          >  2:4215884/1‑93 (MQ=255)

                                                                 gTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGa                        >  2:1350824/1‑93 (MQ=255)

                                                                  tttCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                       >  2:171637/1‑93 (MQ=255)

                                                                   ttcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAg                      <  1:4220500/93‑1 (MQ=255)

                                                                    tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                     >  2:469783/1‑93 (MQ=255)

                                                                    tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                     <  1:3096353/93‑1 (MQ=255)

                                                                    tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAAGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                     >  2:18866/1‑93 (MQ=255)

                                                                          ccGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa               <  2:306559/93‑1 (MQ=255)

                                                                           cGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              <  2:978893/93‑1 (MQ=255)

                                                                           cGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              <  2:4450816/93‑1 (MQ=255)

                                                                           cGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              <  1:3716169/93‑1 (MQ=255)

                                                                           cGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              >  1:90160/1‑93 (MQ=255)

                                                                           cGGAGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc              >  2:3707561/1‑93 (MQ=255)

                                                                            gggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAcc             <  2:182373/93‑1 (MQ=255)

                                                                            gggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAcc             <  1:1672604/93‑1 (MQ=255)

                                                                            gggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCAAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAcc             >  2:2547195/1‑93 (MQ=255)

                                                                             ggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCg            >  1:715647/1‑93 (MQ=255)

                                                                              gggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCgg           >  2:572912/1‑93 (MQ=255)

                                                                               ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          <  1:3611979/93‑1 (MQ=255)

                                                                               ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa          <  2:200/93‑1 (MQ=255)

                                                                                gcgcCCGCACCATTCGTGGCTACAAATACAAATCTAGCGCGCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAt         <  2:4052260/93‑1 (MQ=255)

                                                                                   aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      >  1:3827257/1‑93 (MQ=255)

                                                                                   aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      >  2:1387162/1‑93 (MQ=255)

                                                                                   aCCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGc      <  1:2619119/93‑1 (MQ=255)

                                                                                    ccGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCt     <  1:2687249/93‑1 (MQ=255)

                                                                                     cGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGCTAACCGGATCGCTg    >  1:3983480/1‑93 (MQ=255)

TGTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGGA  >  NC_000913/4441293‑4441472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`