BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	500,613	A→G	E422G (GAG→GGG)	gsk →	inosine‑guanosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	500,613	0	A	G	99.4%	547.0 / ‑5.1	175	E422G (GAG→GGG)	gsk	inosine‑guanosine kinase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (81/93); total (81/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 178 total aligned reads displayed.

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

  tCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGt                                                                                       >  2:3278134/1‑93 (MQ=255)

  tCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGt                                                                                       >  1:3955872/1‑93 (MQ=255)

  tCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGt                                                                                       >  2:282774/1‑93 (MQ=255)

   cGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTg                                                                                      >  1:2412834/1‑93 (MQ=255)

    gTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGa                                                                                     <  2:2450307/93‑1 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                    >  2:267524/1‑93 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                    >  2:3032564/1‑93 (MQ=255)

      tAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAg                                                                                   <  1:3724161/93‑1 (MQ=255)

        gcgcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGAc                                                                                 <  2:4236996/93‑1 (MQ=255)

         cgcAGGTGTGTAAATCTGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACa                                                                                <  2:2523214/93‑1 (MQ=255)

           cAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGc                                                                              <  2:2429354/93‑1 (MQ=255)

           cAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGc                                                                              >  1:2864721/1‑93 (MQ=255)

           cAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGc                                                                              <  1:2663885/93‑1 (MQ=255)

           cAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGc                                                                              <  1:1527287/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                             <  2:4374413/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                             <  1:4435743/93‑1 (MQ=255)

             ggTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCt                                                                            >  2:115644/1‑93 (MQ=255)

               tgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTgg                                                                          <  2:807495/93‑1 (MQ=255)

               tgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTgg                                                                          >  2:3400954/1‑93 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGa                                                                         >  2:3674161/1‑93 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGa                                                                         <  1:3006210/93‑1 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGa                                                                         <  1:90695/93‑1 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGa                                                                         <  1:2308217/93‑1 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGaa                                                                        <  1:1083856/93‑1 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGaa                                                                        >  1:4218694/1‑93 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGaa                                                                        >  1:2611090/1‑93 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAaga                                                                      <  1:1410245/93‑1 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAaga                                                                      <  1:779964/93‑1 (MQ=255)

                    aaaTATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAagag                                                                     <  1:785965/93‑1 (MQ=255)

                    aaaTATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAagag                                                                     >  1:3443303/1‑93 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAagaggc                                                                   >  2:3238350/1‑91 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTc                                                                   <  2:2111927/93‑1 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTc                                                                   >  2:3724451/1‑93 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  >  2:2234510/1‑93 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  <  2:3980230/93‑1 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  >  1:1909372/1‑93 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  <  2:3778518/93‑1 (MQ=255)

                         tGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTa                                                                >  1:1244257/1‑93 (MQ=255)

                          gCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTAc                                                               <  1:347603/93‑1 (MQ=255)

                          gCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTAc                                                               <  2:2145433/93‑1 (MQ=255)

                            tAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTg                                                             >  1:1237076/1‑93 (MQ=255)

                             aaCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTgg                                                            >  1:3311080/1‑93 (MQ=255)

                             aaCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTgg                                                            <  2:2716113/93‑1 (MQ=255)

                             aaCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTgg                                                            <  1:3717552/93‑1 (MQ=255)

                               ccGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGa                                                          <  2:1310730/93‑1 (MQ=255)

                                   gtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGt                                                      <  2:1616010/93‑1 (MQ=255)

                                      atcTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTaa                                                   <  1:3381162/91‑1 (MQ=255)

                                      aGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTaa                                                   >  1:1893972/1‑93 (MQ=255)

                                      aGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTaa                                                   >  2:48022/1‑93 (MQ=255)

                                       gCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAg                                                  >  2:1745853/1‑93 (MQ=255)

                                        cTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGt                                                 <  2:3593713/93‑1 (MQ=255)

                                        cTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGt                                                 >  1:2213786/1‑93 (MQ=255)

                                          aTCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTa                                               <  2:1149201/93‑1 (MQ=255)

                                           tCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAt                                              >  1:2453664/1‑93 (MQ=255)

                                           tCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAt                                              <  1:3904534/93‑1 (MQ=255)

                                              ggTACTGAACCAGCATTCTCCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgt                                           <  1:2697051/93‑1 (MQ=255)

                                              ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgt                                           <  2:3539841/93‑1 (MQ=255)

                                               gTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtc                                          >  2:957224/1‑93 (MQ=255)

                                               gTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtc                                          <  2:580798/93‑1 (MQ=255)

                                               gTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtc                                          <  2:1991490/93‑1 (MQ=255)

                                                tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtcg                                         <  2:3457611/93‑1 (MQ=255)

                                                tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtcg                                         >  1:4121124/1‑93 (MQ=255)

                                                tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtcg                                         >  2:1487697/1‑93 (MQ=255)

                                                tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtcg                                         >  1:818614/1‑93 (MQ=255)

                                                 aCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCgg                                        >  2:2024882/1‑93 (MQ=255)

                                                 aCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGACGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCgg                                        <  1:3061672/93‑1 (MQ=255)

                                                  cTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGGCTTACTGGGATCGTTAAGTTATCGTCGGt                                       >  1:1001526/1‑93 (MQ=255)

                                                    gAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTc                                     >  1:4472553/1‑93 (MQ=255)

                                                     aaCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGGGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAACTTATTGTCGGTTCg                                    >  2:3470699/1‑93 (MQ=255)

                                                      aCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGt                                   >  2:4638869/1‑93 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  >  1:3829392/1‑93 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAg                                 <  2:4668626/93‑1 (MQ=255)

                                                         aGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAgg                                >  2:3685122/1‑93 (MQ=255)

                                                           cATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGcc                              <  1:1434220/93‑1 (MQ=255)

                                                           cATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGcc                              >  2:4064738/1‑93 (MQ=255)

                                                            aTTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCa                             >  1:2996835/1‑93 (MQ=255)

                                                            aTTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGCGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCa                             >  2:1653973/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  1:470111/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  1:3364884/1‑93 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTATGCCAGa                           >  1:2835515/1‑93 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGa                           <  1:43845/93‑1 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGa                           <  2:3364541/93‑1 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGa                           >  2:4569966/1‑93 (MQ=255)

                                                                aCCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATa                         >  1:140843/1‑93 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  2:49991/93‑1 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  2:4168209/93‑1 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  1:507174/93‑1 (MQ=255)

                                                                  cTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAg                       <  2:1278205/93‑1 (MQ=255)

                                                                    cGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAggg                     >  1:3191363/1‑92 (MQ=255)

                                                                    cGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGc                     >  2:4181438/1‑93 (MQ=255)

                                                                       ttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGtt                  >  1:1606815/1‑93 (MQ=255)

                                                                        tAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTc                 <  1:3992963/93‑1 (MQ=255)

                                                                         aaCGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCa                <  1:2109962/93‑1 (MQ=255)

                                                                         aaCGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCa                >  2:3086086/1‑93 (MQ=255)

                                                                         aaCGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCa                >  2:111041/1‑93 (MQ=255)

                                                                          aCGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAc               <  1:3878741/93‑1 (MQ=255)

                                                                                  cTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATc       <  2:696276/93‑1 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      <  2:2985573/93‑1 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      <  2:776612/93‑1 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      <  1:2946059/93‑1 (MQ=255)

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`