BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,365	A→G	L173P (CTT→CCT)	fecB ←	FecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,365	0	A	G	99.5%	617.7 / NA	198	L173P (CTT→CCT)	fecB	FecB
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (110/87); total (110/88)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA  >  NC_000913/4511275‑4511454

gCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgc                                                                                         <  2:646826/93‑1 (MQ=255)

 ccAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgct                                                                                        <  2:1719062/93‑1 (MQ=255)

     cACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgatggct                                                                                    >  1:3216210/1‑93 (MQ=255)

      aCGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgctggctg                                                                                   >  1:3875053/1‑93 (MQ=255)

      aCGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGgctggctg                                                                                   >  1:1266257/1‑93 (MQ=255)

          tGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGccc                                                                               >  2:4296807/1‑93 (MQ=255)

           gCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCa                                                                              >  1:435600/1‑93 (MQ=255)

             cGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACt                                                                            >  1:2994016/1‑93 (MQ=255)

             cGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACt                                                                            <  2:196468/93‑1 (MQ=255)

               gTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgc                                                                          >  2:4496032/1‑93 (MQ=255)

               gTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgc                                                                          <  1:393072/93‑1 (MQ=255)

                tCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgcg                                                                         <  1:1023473/93‑1 (MQ=255)

                tCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgcg                                                                         >  2:3622348/1‑93 (MQ=255)

                 ccAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTgcgc                                                                        >  1:4598493/1‑93 (MQ=255)

                    ggTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCAt                                                                     >  1:407989/1‑93 (MQ=255)

                      tctcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATcc                                                                   <  1:4558925/93‑1 (MQ=255)

                      tctcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATcc                                                                   <  1:2790311/93‑1 (MQ=255)

                       ctcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCct                                                                  <  2:1479921/93‑1 (MQ=255)

                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 <  2:2295795/93‑1 (MQ=255)

                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 >  1:3431054/1‑93 (MQ=255)

                        tcCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctc                                                                 <  2:1507701/93‑1 (MQ=255)

                        tcCCGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATGctc                                                                 >  2:3656453/1‑93 (MQ=255)

                          cTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctc                                                               <  1:2678007/93‑1 (MQ=255)

                          cTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctc                                                               <  2:1822198/93‑1 (MQ=255)

                           tGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctct                                                              >  1:1862176/1‑93 (MQ=255)

                           tGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCctctct                                                              >  1:4257832/1‑93 (MQ=255)

                            gAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCCCTGCGCCATCCTCTCtt                                                             >  2:3988695/1‑93 (MQ=255)

                             aGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCttt                                                            <  1:3949121/93‑1 (MQ=255)

                              gTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCCCGCGTGTCCCTTTGGGGGGCTGGCTGGCCCCCTGCGCCATCCCCTCtttt                                                           >  1:3312699/1‑92 (MQ=255)

                              gTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCCTCCTCTCTTTa                                                           >  1:2471169/1‑93 (MQ=255)

                              gTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCtttt                                                           >  2:4179923/1‑92 (MQ=255)

                               tATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAt                                                          >  2:844570/1‑93 (MQ=255)

                               tATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAt                                                          >  2:2581152/1‑93 (MQ=255)

                                aTGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtg                                                         >  2:454254/1‑93 (MQ=255)

                                aTGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtg                                                         <  1:295093/93‑1 (MQ=255)

                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        <  2:4660854/93‑1 (MQ=255)

                                 tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgt                                                        >  2:1245176/1‑93 (MQ=255)

                                  gCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgtt                                                       >  1:2516747/1‑93 (MQ=255)

                                  gCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgtt                                                       <  1:1589533/93‑1 (MQ=255)

                                    aGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTAtgttgt                                                     >  2:282998/1‑93 (MQ=255)

                                     ggTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATgttgtt                                                    >  2:2948716/1‑93 (MQ=255)

                                       ttGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGTGCCATCCTCTCTTTATGTTGTTcc                                                  >  2:1605632/1‑93 (MQ=255)

                                       ttGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTcc                                                  <  2:3199981/93‑1 (MQ=255)

                                       ttGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTcc                                                  <  1:1651594/93‑1 (MQ=255)

                                        tGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCa                                                 <  1:614560/93‑1 (MQ=255)

                                         gAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAg                                                >  2:1912736/1‑93 (MQ=255)

                                         gAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAg                                                >  2:3884445/1‑93 (MQ=255)

                                          aaTTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGa                                               <  2:2082674/93‑1 (MQ=255)

                                           aTTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGAc                                              >  1:1179705/1‑93 (MQ=255)

                                            ttGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACg                                             >  1:3605739/1‑93 (MQ=255)

                                            ttGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACg                                             >  1:4486186/1‑93 (MQ=255)

                                              gCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTg                                           >  2:555050/1‑93 (MQ=255)

                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         >  1:720087/1‑93 (MQ=255)

                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         >  1:1910576/1‑93 (MQ=255)

                                                tGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGcc                                         >  2:2646430/1‑93 (MQ=255)

                                                 gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        >  1:3131212/1‑93 (MQ=255)

                                                 gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        <  2:2434682/93‑1 (MQ=255)

                                                 gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCt                                        >  1:1992166/1‑93 (MQ=255)

                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       >  2:3407894/1‑93 (MQ=255)

                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       >  2:4222590/1‑93 (MQ=255)

                                                  ttCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTg                                       >  1:3651225/1‑93 (MQ=255)

                                                    cgcgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCa                                     <  1:4348506/93‑1 (MQ=255)

                                                    cgcgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCa                                     <  1:311709/93‑1 (MQ=255)

                                                     gcgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAt                                    >  2:2803124/1‑93 (MQ=255)

                                                      cgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtc                                   >  1:1975968/1‑93 (MQ=255)

                                                      cgTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtc                                   >  1:775399/1‑93 (MQ=255)

                                                       gTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtct                                  >  1:4469823/1‑93 (MQ=255)

                                                        tGATGTGCCAAAGGCCACGCGTGTGCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctc                                 >  2:4517993/1‑93 (MQ=255)

                                                        tGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctc                                 >  2:1558209/1‑93 (MQ=255)

                                                          aTGTGCTAAAGGCCACGCGTGTCCCTTTGGGAGGCTTGCTGGCCCCCTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctctc                               <  2:2615760/93‑1 (MQ=255)

                                                          aTGTGCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCAtctctc                               >  1:1006278/1‑93 (MQ=255)

                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             <  1:3908809/93‑1 (MQ=255)

                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             >  1:4173687/1‑93 (MQ=255)

                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             <  1:766325/93‑1 (MQ=255)

                                                            gtgCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGc                             <  2:2893884/93‑1 (MQ=255)

                                                              gCCAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCtt                           >  1:1077867/1‑93 (MQ=255)

                                                                cAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCTCtttt                         >  2:1188803/1‑93 (MQ=255)

                                                                cAAAGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCtttt                         >  1:959565/1‑93 (MQ=255)

                                                                 aaaGGCCGCGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        <  1:229114/93‑1 (MQ=255)

                                                                 aaaGGCCACGCGTGTCCCTTTGGGAGGTTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        <  1:827830/93‑1 (MQ=255)

                                                                 aaaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCttttt                        >  2:34807/1‑93 (MQ=255)

                                                                  aaGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTa                       <  1:353103/93‑1 (MQ=255)

                                                                   aGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAc                      >  2:3820524/1‑93 (MQ=255)

                                                                   aGGCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAc                      >  1:1348035/1‑93 (MQ=255)

                                                                    ggCCACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTAcc                     >  2:1934273/1‑93 (MQ=255)

                                                                     gCCACCCGTTTCCCTTTGGGAGGCTGGCTGGGCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGCTGTGCCTGCATCTCTCGCttttttact                    >  2:3943963/1‑89 (MQ=255)

                                                                      ccACGCGTGTCCCTTTGGGAGTCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACcca                   <  2:756014/93‑1 (MQ=255)

                                                                      ccACGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACcca                   <  2:28192/93‑1 (MQ=255)

                                                                        aCGCGTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACccacc                 >  1:947596/1‑93 (MQ=255)

                                                                         cgcgTGTCCCTTTGGGAGGCTGGCGGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACccacca                <  1:1215572/93‑1 (MQ=255)

                                                                          gcgTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCAt               <  2:2456043/93‑1 (MQ=255)

                                                                           cgTGTCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCAtt              <  2:375379/93‑1 (MQ=255)

                                                                               tCCCTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGc          <  1:2211265/93‑1 (MQ=255)

                                                                                cccTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGcc         <  2:250736/93‑1 (MQ=255)

                                                                                cccTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGcc         >  1:3481276/1‑93 (MQ=255)

                                                                                  cTTTGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCga       <  2:3277730/93‑1 (MQ=255)

                                                                                     tGGGAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatga    <  1:214532/93‑1 (MQ=255)

                                                                                      gggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatgat   <  1:1322394/93‑1 (MQ=255)

                                                                                      gggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatgat   >  2:2135891/1‑93 (MQ=255)

                                                                                       ggAGGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATa  <  2:1009729/93‑1 (MQ=255)

GCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATA  >  NC_000913/4511275‑4511454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`