BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,170	T→C	I502I (ATT→ATC)	fimD →	FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,170	0	T	C	100.0%	744.0 / NA	231	I502I (ATT→ATC)	fimD	FimD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (130/101); total (130/101)

Only 100 of 233 total aligned reads displayed.

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGa                                                                                         >  2:2880034/1‑93 (MQ=255)

 aaTATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaa                                                                                        >  1:1859882/1‑93 (MQ=255)

  atatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaaa                                                                                       <  1:73056/93‑1 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      <  2:3417454/93‑1 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      >  2:4082116/1‑93 (MQ=255)

    atTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAaca                                                                                     <  1:1224334/93‑1 (MQ=255)

    atTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAaca                                                                                     >  1:1389738/1‑93 (MQ=255)

     ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAacac                                                                                    <  1:1480922/93‑1 (MQ=255)

     ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAacac                                                                                    <  2:3630745/93‑1 (MQ=255)

     ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAacac                                                                                    >  2:3783290/1‑93 (MQ=255)

      tCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAAcaca                                                                                   <  2:4243483/93‑1 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  >  2:2384076/1‑93 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                >  2:1535742/1‑93 (MQ=255)

           tAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACg                                                                              >  1:179011/1‑93 (MQ=255)

             gTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGa                                                                            <  2:1850275/93‑1 (MQ=255)

              tGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAg                                                                           >  2:4350631/1‑93 (MQ=255)

                  ttACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGGCGGAGTTAt                                                                       <  1:4143940/93‑1 (MQ=255)

                  ttACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAt                                                                       <  2:743454/93‑1 (MQ=255)

                   tACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAtt                                                                      <  1:2328051/93‑1 (MQ=255)

                   tACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAtt                                                                      <  2:3247149/93‑1 (MQ=255)

                   tACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAtt                                                                      >  1:1006260/1‑93 (MQ=255)

                    aCCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTc                                                                     >  1:1089104/1‑93 (MQ=255)

                       gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAgg                                                                  >  2:2248297/1‑93 (MQ=255)

                        ttATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGt                                                                 >  1:2450092/1‑93 (MQ=255)

                          aTTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTa                                                               >  2:2807600/1‑93 (MQ=255)

                          aTTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTa                                                               <  2:2035755/93‑1 (MQ=255)

                           ttCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTaa                                                              >  2:1439117/1‑93 (MQ=255)

                            tCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAg                                                             <  1:1457230/93‑1 (MQ=255)

                             cGAGCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCTAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGc                                                            <  1:3433153/93‑1 (MQ=255)

                                ccAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTACGCCGa                                                         >  2:1346193/1‑93 (MQ=255)

                                ccAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGa                                                         <  1:1063562/93‑1 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      >  2:3568795/1‑93 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      >  1:3864880/1‑93 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTAATTCAGGTTACGCCGAAAt                                                      <  2:4580501/93‑1 (MQ=255)

                                    cGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAtt                                                     >  1:2028848/1‑93 (MQ=255)

                                    cGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAtt                                                     <  2:3047568/93‑1 (MQ=255)

                                      gATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGAAGTTATTCAGGTTAAGCCGAAATTCa                                                   >  1:3846924/1‑93 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATGCAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  <  1:2764735/93‑1 (MQ=255)

                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 <  1:4049829/93‑1 (MQ=255)

                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 <  1:3041458/93‑1 (MQ=255)

                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 >  1:2065081/1‑93 (MQ=255)

                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 <  1:703926/93‑1 (MQ=255)

                                         atTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCg                                                <  2:2501794/93‑1 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               <  2:2517509/93‑1 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               >  2:2640030/1‑93 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               >  2:1673372/1‑93 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               <  2:2673907/93‑1 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               >  2:292461/1‑93 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              <  1:1325279/93‑1 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              <  2:18787/93‑1 (MQ=255)

                                             tAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTa                                            >  1:2379640/1‑93 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         >  1:3770400/1‑93 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         <  1:3916832/93‑1 (MQ=255)

                                                  tCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACa                                       >  1:2220093/1‑93 (MQ=255)

                                                   cGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                      >  1:58578/1‑93 (MQ=255)

                                                   cGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                      <  2:1610871/93‑1 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     >  1:2866952/1‑93 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   >  2:1104124/1‑93 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   >  1:3799767/1‑93 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   >  1:2649908/1‑93 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   <  2:4408053/93‑1 (MQ=255)

                                                       gATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTc                                  >  2:2803505/1‑93 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                >  2:3988192/1‑93 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                >  2:550383/1‑93 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                <  1:3556320/93‑1 (MQ=255)

                                                           caacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCtt                              >  1:3471018/1‑93 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             <  2:2325421/93‑1 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             <  1:439642/93‑1 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             <  2:73513/93‑1 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGGTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             >  2:1403660/1‑93 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            <  1:92720/93‑1 (MQ=255)

                                                               aTACAGTCGAATGAATGGCTACAAGATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                          <  2:4597127/93‑1 (MQ=255)

                                                               aTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                          >  2:3356060/1‑93 (MQ=255)

                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  1:156357/1‑93 (MQ=255)

                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      >  1:557653/1‑93 (MQ=255)

                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      <  2:502640/93‑1 (MQ=255)

                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      <  2:2657645/93‑1 (MQ=255)

                                                                    gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                     >  1:2495306/1‑93 (MQ=255)

                                                                    gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                     <  1:542330/93‑1 (MQ=255)

                                                                    gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                     >  2:4516002/1‑93 (MQ=255)

                                                                      caaattaatgGCTACAACATCGAAACACAGGACGAAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgc                   <  1:2503777/91‑1 (MQ=255)

                                                                      cGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCATGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgc                   >  1:4439449/1‑93 (MQ=255)

                                                                      cGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgc                   <  2:558191/93‑1 (MQ=255)

                                                                      cGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgc                   <  1:1743827/93‑1 (MQ=255)

                                                                      cGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgc                   <  1:1949138/93‑1 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:3843745/1‑93 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  1:459917/1‑93 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  2:1460292/1‑93 (MQ=255)

                                                                           gaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaa              >  2:277337/1‑93 (MQ=255)

                                                                             atgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAt            >  1:4182892/1‑93 (MQ=255)

                                                                              tgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAtt           <  2:2177722/93‑1 (MQ=255)

                                                                               ggCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTa          >  1:3786825/1‑93 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  2:840922/93‑1 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         >  2:3966746/1‑93 (MQ=255)

                                                                                    caacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACt     >  2:3805622/1‑93 (MQ=255)

                                                                                     aacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACGACTc    >  1:3185327/1‑93 (MQ=255)

                                                                                      acaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAGCCTCGCTTATAACAAACGCGGGAAATTACAACTCa   <  1:3220960/93‑1 (MQ=255)

                                                                                      acaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCa   >  2:1208105/1‑93 (MQ=255)

                                                                                      acaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCa   >  1:1432761/1‑93 (MQ=255)

                                                                                       caTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc  <  2:3266522/93‑1 (MQ=255)

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`