BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	504,799	A→G	Y221C (TAC→TGC)	ushA →	UDP‑sugar hydrolase (5'‑nucleotidase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	504,799	0	A	G	100.0%	456.3 / NA	143	Y221C (TAC→TGC)	ushA	UDP‑sugar hydrolase (5'‑nucleotidase)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (86/57); total (86/57)

Only 100 of 147 total aligned reads displayed.

AGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTG  >  NC_000913/504709‑504888

aGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCg                                                                                         >  1:642670/1‑93 (MQ=255)

   ccgccgATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATa                                                                                      >  2:1372779/1‑93 (MQ=255)

    cgccgATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATaa                                                                                     >  2:2889211/1‑93 (MQ=255)

     gccgATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAAt                                                                                    <  1:2431607/93‑1 (MQ=255)

       cgATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATgg                                                                                  >  1:514340/1‑93 (MQ=255)

       cgATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATgg                                                                                  >  1:1634859/1‑93 (MQ=255)

        gATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGt                                                                                 <  1:2304210/93‑1 (MQ=255)

         aTGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTg                                                                                >  1:2205564/1‑93 (MQ=255)

         aTGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTg                                                                                <  2:2187390/93‑1 (MQ=255)

           gaagcgaagcTGGTGATTCAGGAGCTGTAACAGACAGAAATGCCAGACATTATTATCGCGGCTATCCATATGGGGCATTGCGATAATGGTGAg                                                                              >  2:4686184/1‑93 (MQ=255)

           gaagcgaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAg                                                                              <  2:3445076/93‑1 (MQ=255)

            aagcgaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGc                                                                             >  2:779392/1‑93 (MQ=255)

            aagcgaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGc                                                                             >  1:2084480/1‑93 (MQ=255)

            aagcgaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGc                                                                             >  1:1751365/1‑93 (MQ=255)

             agcgaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCa                                                                            <  1:1440002/93‑1 (MQ=255)

              gcgaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCAc                                                                           >  1:4563853/1‑93 (MQ=255)

                gaagcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACgg                                                                         <  2:2920354/93‑1 (MQ=255)

                  agcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGct                                                                       >  2:4021128/1‑93 (MQ=255)

                  agcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGct                                                                       >  1:2885377/1‑93 (MQ=255)

                   gcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGctc                                                                      >  2:1909658/1‑93 (MQ=255)

                   gcTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGctc                                                                      >  2:3811998/1‑93 (MQ=255)

                    cTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGctct                                                                     >  1:3151641/1‑93 (MQ=255)

                      ggTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTaa                                                                   <  2:2779439/93‑1 (MQ=255)

                      ggTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTaa                                                                   >  1:1255130/1‑93 (MQ=255)

                      ggTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTaa                                                                   <  1:1932969/93‑1 (MQ=255)

                        tGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACg                                                                 <  1:916361/93‑1 (MQ=255)

                         gATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGc                                                                >  2:3377261/1‑93 (MQ=255)

                         gATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGc                                                                >  2:3622290/1‑93 (MQ=255)

                             cAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCg                                                            <  1:4663604/93‑1 (MQ=255)

                             cAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCg                                                            <  1:4120400/93‑1 (MQ=255)

                              aGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCgg                                                           <  1:4588012/93‑1 (MQ=255)

                               ggAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCggg                                                          >  2:1464227/1‑93 (MQ=255)

                               ggAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCggg                                                          <  1:2541647/93‑1 (MQ=255)

                                  gCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGa                                                       >  1:232097/1‑93 (MQ=255)

                                  gCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGa                                                       <  1:518391/93‑1 (MQ=255)

                                     gCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgt                                                    >  2:3726875/1‑93 (MQ=255)

                                     gCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgt                                                    <  1:1451936/93‑1 (MQ=255)

                                     gCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgt                                                    >  1:3651410/1‑93 (MQ=255)

                                     gCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgt                                                    <  2:2067608/93‑1 (MQ=255)

                                     gCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgt                                                    <  1:1045608/93‑1 (MQ=255)

                                     gCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgt                                                    >  1:3069936/1‑93 (MQ=255)

                                      cAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgtg                                                   >  2:3796852/1‑93 (MQ=255)

                                      cAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGAtgtg                                                   <  1:1368993/93‑1 (MQ=255)

                                        acagacagAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTggg                                                 >  2:577290/1‑92 (MQ=255)

                                        acagacagAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGga                                                 <  1:1692313/93‑1 (MQ=255)

                                        acagacagAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGga                                                 <  2:107936/93‑1 (MQ=255)

                                         cagacagaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGgag                                                >  1:3236375/1‑93 (MQ=255)

                                          agacagaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGgaga                                               >  2:3310653/1‑93 (MQ=255)

                                          agacagaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGgaga                                               <  2:1174614/93‑1 (MQ=255)

                                             cagaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATgg                                            >  2:4432602/1‑93 (MQ=255)

                                             cagaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATgg                                            >  2:2372748/1‑93 (MQ=255)

                                               gaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCa                                          >  2:3011157/1‑93 (MQ=255)

                                               gaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCa                                          >  2:4652098/1‑93 (MQ=255)

                                               gaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCa                                          >  2:3501514/1‑93 (MQ=255)

                                               gaAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCa                                          <  1:3042558/93‑1 (MQ=255)

                                                aaaaGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCAc                                         >  2:4064090/1‑93 (MQ=255)

                                                 aaaGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGTGATGTGGAGATGGCAcg                                        <  2:1364971/93‑1 (MQ=255)

                                                  aaGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCAcgc                                       <  2:2638259/93‑1 (MQ=255)

                                                   aGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCAcgcg                                      >  2:3557195/1‑93 (MQ=255)

                                                       aGACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGct                                  >  1:492323/1‑93 (MQ=255)

                                                        gACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctg                                 >  1:4116081/1‑93 (MQ=255)

                                                        gACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctg                                 >  2:4236777/1‑93 (MQ=255)

                                                        gACATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctg                                 >  1:4420461/1‑93 (MQ=255)

                                                          cATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcc                               <  1:720944/93‑1 (MQ=255)

                                                          cATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcc                               <  1:1768846/93‑1 (MQ=255)

                                                          cATTATTATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcc                               <  2:3104919/93‑1 (MQ=255)

                                                           attattATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcct                              >  1:1519717/1‑93 (MQ=255)

                                                           attattATCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcct                              >  2:3223570/1‑93 (MQ=255)

                                                            ttattaTCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcctg                             >  1:4103537/1‑93 (MQ=255)

                                                             tattatCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGctgcctgc                            >  2:1811995/1‑93 (MQ=255)

                                                               ttatCGCGGCGACCCATATGAGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCg                          >  2:2667561/1‑93 (MQ=255)

                                                                 atCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGa                        >  2:4572836/1‑93 (MQ=255)

                                                                 atCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGa                        >  2:970456/1‑93 (MQ=255)

                                                                  tCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGAt                       <  2:546474/93‑1 (MQ=255)

                                                                  tCGCGGCGACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGAt                       >  1:959171/1‑93 (MQ=255)

                                                                     cggcgACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTATCGCACCGGTCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGc                    <  2:1415377/93‑1 (MQ=255)

                                                                     cggcgACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGc                    <  1:4408387/93‑1 (MQ=255)

                                                                      ggcgACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCt                   <  1:3661594/93‑1 (MQ=255)

                                                                      ggcgACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGCGGAGATGGCACGCGCGCTGCCTGCCGGATCGCt                   >  1:292070/1‑93 (MQ=255)

                                                                       gcgACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTg                  >  1:2773175/1‑93 (MQ=255)

                                                                        cgACCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTgg                 <  2:4294566/93‑1 (MQ=255)

                                                                         gACCCATATGGGGCATTGCGATAACGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGc                <  2:3998225/93‑1 (MQ=255)

                                                                          aCCCATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCg               >  1:3780989/1‑93 (MQ=255)

                                                                            ccATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgat             >  1:1540672/1‑93 (MQ=255)

                                                                            ccATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgat             >  2:2372987/1‑93 (MQ=255)

                                                                             cATATGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGCGCGATGTGGAGATGGCAACCGCGCTGCCTGCCGGATCGCTGGCggtg            >  2:3255856/1‑93 (MQ=255)

                                                                              atgtGGGACATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgatga           <  1:2752653/90‑1 (MQ=255)

                                                                              atatGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgatga           >  2:4129868/1‑93 (MQ=255)

                                                                               tatGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgatgat          <  2:1565075/93‑1 (MQ=255)

                                                                               tatGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgatgat          <  2:210550/93‑1 (MQ=255)

                                                                               tatGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgatgat          >  1:1827663/1‑93 (MQ=255)

                                                                               tatGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgatgat          <  2:2236068/93‑1 (MQ=255)

                                                                               tatGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCgatgat          >  1:1728735/1‑93 (MQ=255)

                                                                                 tGGGGCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcg        >  2:3344906/1‑93 (MQ=255)

                                                                                  ggggCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgt       <  1:3869797/93‑1 (MQ=255)

                                                                                   gggCATTGCGATAATGGTGAGCACGGCTCTAACGCCCCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgtc      <  2:4178860/93‑1 (MQ=255)

                                                                                   gggCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgtc      <  2:764223/93‑1 (MQ=255)

                                                                                    ggCATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGAtcgtcg     >  2:967141/1‑93 (MQ=255)

                                                                                      cATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCggt   <  2:1710463/93‑1 (MQ=255)

                                                                                      cATTGCGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCggt   <  2:1480972/93‑1 (MQ=255)

AGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATATGGGGCATTACGATAATGGTGAGCACGGCTCTAACGCACCGGGCGATGTGGAGATGGCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTG  >  NC_000913/504709‑504888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`