BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,582,861	A→G	S509P (TCC→CCC)	hsdR ←	endonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,582,861	0	A	G	99.5%	599.5 / ‑4.9	192	S509P (TCC→CCC)	hsdR	endonuclease R
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (93/98); total (94/98)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 201 total aligned reads displayed.

CGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGA  >  NC_000913/4582771‑4582950

cGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCt                                                                                         <  1:2296565/93‑1 (MQ=255)

 gTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTg                                                                                        >  1:4366305/1‑93 (MQ=255)

   tAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGa                                                                                      <  2:4012993/93‑1 (MQ=255)

   tAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGa                                                                                      >  1:4643724/1‑93 (MQ=255)

    aaTACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGAt                                                                                     <  1:338351/93‑1 (MQ=255)

    aaTACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTAGGGCTGGAt                                                                                     >  2:864196/1‑93 (MQ=255)

     aTACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATc                                                                                    <  1:1505777/93‑1 (MQ=255)

     aTACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATc                                                                                    <  2:788429/93‑1 (MQ=255)

     aTACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATc                                                                                    >  2:3657308/1‑93 (MQ=255)

      tACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCa                                                                                   >  2:2527703/1‑93 (MQ=255)

        cgcgTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAgg                                                                                 <  1:1728365/93‑1 (MQ=255)

          cgTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCg                                                                               <  2:4429842/93‑1 (MQ=255)

          cgTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCg                                                                               <  1:3978867/93‑1 (MQ=255)

          cgTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCg                                                                               >  1:4007027/1‑93 (MQ=255)

           gTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACTCTGGGGCTGGATCAGGTgg                                                                              >  1:158934/1‑93 (MQ=255)

           gTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCgg                                                                              >  2:933996/1‑93 (MQ=255)

              tCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAc                                                                           <  1:1564802/93‑1 (MQ=255)

              tCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAc                                                                           >  1:1184646/1‑93 (MQ=255)

              tCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCCGAAc                                                                           >  2:3702582/1‑93 (MQ=255)

              tCTTCAAACGCGCCCAGCGCCTGGTCGCCAAGAGAACGGCGGTCGAGAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAc                                                                           >  2:3780071/1‑93 (MQ=255)

                 tCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGGCGAAcatc                                                                        >  2:1390393/1‑93 (MQ=255)

                 tCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGGAcata                                                                        >  1:25786/1‑92 (MQ=255)

                 tCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAcatc                                                                        >  2:1797881/1‑93 (MQ=255)

                 tCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAcatc                                                                        >  2:1506961/1‑93 (MQ=255)

                 tCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAcatc                                                                        <  1:2437967/93‑1 (MQ=255)

                   aaaCGCGCCCAGCGCCTGTTCGCCAAGAGAACGTCAGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAcatcat                                                                      <  2:4485993/93‑1 (MQ=255)

                   aaaCGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAcatcat                                                                      <  1:497176/93‑1 (MQ=255)

                   aaaCGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAcatcat                                                                      <  1:4322461/93‑1 (MQ=255)

                   aaaCGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAAcatcat                                                                      >  1:3253131/1‑93 (MQ=255)

                    aaCGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATg                                                                     >  1:3668607/1‑93 (MQ=255)

                    aaCGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATg                                                                     >  1:1993700/1‑93 (MQ=255)

                     aCGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATgg                                                                    <  1:99183/93‑1 (MQ=255)

                      cgcgCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGc                                                                   <  1:3807351/93‑1 (MQ=255)

                        cgcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGa                                                                 >  2:4657385/1‑93 (MQ=255)

                         gcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATGAGGCGGAACATCATGGCGAt                                                                >  1:1762819/1‑93 (MQ=255)

                         gcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGAt                                                                <  2:2694382/93‑1 (MQ=255)

                         gcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGAt                                                                <  2:3095760/93‑1 (MQ=255)

                           ccAGCGCCTGTTCGCCAAGAGAACGTCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTg                                                              <  2:1693702/93‑1 (MQ=255)

                           ccAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGAttc                                                              >  1:3487438/1‑92 (MQ=255)

                             aGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGcc                                                            >  1:2701525/1‑93 (MQ=255)

                              gcgcCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCg                                                           >  1:3715171/1‑93 (MQ=255)

                              gcgcCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCg                                                           >  2:4102470/1‑93 (MQ=255)

                              gcgcCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCg                                                           >  2:3297884/1‑93 (MQ=255)

                               cgcCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGt                                                          <  2:606029/93‑1 (MQ=255)

                                 ccTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTAc                                                        <  2:4137434/93‑1 (MQ=255)

                                  cTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACg                                                       >  1:4554167/1‑93 (MQ=255)

                                  cTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACg                                                       <  1:179739/93‑1 (MQ=255)

                                   tGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACgg                                                      >  1:927174/1‑93 (MQ=255)

                                    gTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACggg                                                     >  2:1568228/1‑93 (MQ=255)

                                     ttCGCCAAGAGAACGGAGGTCAACAAGGAAGAGAATGCTTCTAAAACGCTGGGGCTGGATCAGGCGCAACATCATGGCGATTGCCGTACggga                                                    <  2:3169497/93‑2 (MQ=255)

                                        gCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGtttt                                                 >  1:2097383/1‑93 (MQ=255)

                                         ccAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTta                                                >  2:2143494/1‑93 (MQ=255)

                                         ccAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTta                                                >  2:1332081/1‑93 (MQ=255)

                                          cAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtac                                               >  1:2497364/1‑93 (MQ=255)

                                          cAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtac                                               >  2:2018136/1‑93 (MQ=255)

                                           aaGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtacc                                              <  2:23665/93‑1 (MQ=255)

                                            agagAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtaccg                                             >  1:2793586/1‑93 (MQ=255)

                                              agaACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtaccggt                                           <  2:739699/93‑1 (MQ=255)

                                               gaACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtaccggta                                          <  2:3019195/93‑1 (MQ=255)

                                               gaACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtaccggga                                          >  1:1676558/1‑91 (MQ=255)

                                                aaCGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtaccggtac                                         >  2:1287446/1‑93 (MQ=255)

                                                   ggcggTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTtaccggtaccgg                                      <  2:1084116/93‑1 (MQ=255)

                                                    gcggTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTaccggtaccggt                                     <  2:2069657/93‑1 (MQ=255)

                                                       gTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgc                                  >  2:1191938/1‑93 (MQ=255)

                                                        tCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgcc                                 >  2:339448/1‑93 (MQ=255)

                                                         cGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgcca                                <  2:969692/93‑1 (MQ=255)

                                                         cGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgcca                                >  1:1103774/1‑93 (MQ=255)

                                                         cGACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgcca                                <  2:757344/93‑1 (MQ=255)

                                                          gACAAGGAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGccgccat                               >  1:4155859/1‑93 (MQ=255)

                                                              aGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgccatcgcc                           >  1:66806/1‑93 (MQ=255)

                                                               ggAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgccatcgcca                          >  2:2783368/1‑93 (MQ=255)

                                                               ggAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGtcgccatcgcca                          <  1:3189315/93‑1 (MQ=255)

                                                               ggAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCAGGGCGATTGCCCTACGGGTTTTACCGGTACCGGtcgccatcgcca                          <  2:2913994/93‑1 (MQ=255)

                                                                gAAGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCcag                         >  2:2193961/1‑93 (MQ=255)

                                                                 aaGAGAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc                        >  1:3434197/1‑93 (MQ=255)

                                                                  agagAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca                       >  2:8935/1‑93 (MQ=255)

                                                                  agagAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca                       <  1:535353/93‑1 (MQ=255)

                                                                  agagAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca                       >  2:469769/1‑93 (MQ=255)

                                                                  agagAATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca                       <  2:3546098/93‑1 (MQ=255)

                                                                   gagaATGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagcag                      <  1:1873496/93‑1 (MQ=255)

                                                                      aaTGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAt                   <  1:3460652/93‑1 (MQ=255)

                                                                        tGCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtct                 <  2:4280853/93‑1 (MQ=255)

                                                                         gCGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtctc                >  2:2060567/1‑93 (MQ=255)

                                                                          cGTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATctct               <  1:2738350/93‑1 (MQ=255)

                                                                           gTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCtt              >  1:3240454/1‑93 (MQ=255)

                                                                           gTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCtt              <  1:2399402/93‑1 (MQ=255)

                                                                           gTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCtt              <  1:4520881/93‑1 (MQ=255)

                                                                           gTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCtt              >  1:2613314/1‑93 (MQ=255)

                                                                           gTTTAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCtt              >  2:3462630/1‑93 (MQ=255)

                                                                             ttAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgt            <  1:3845612/93‑1 (MQ=255)

                                                                             ttAAAACGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgt            <  1:3522029/93‑1 (MQ=255)

                                                                               aaaaCGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCTCCAGCAGGATATCttgttg          <  2:4566672/93‑1 (MQ=255)

                                                                                  aCGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGccc       >  1:2879402/1‑93 (MQ=255)

                                                                                  aCGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGccc       >  1:854646/1‑93 (MQ=255)

                                                                                  aCGCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGccc       <  2:1542654/93‑1 (MQ=255)

                                                                                    gCTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCt     <  2:4633217/93‑1 (MQ=255)

                                                                                     cTGGGGCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCtt    >  2:3530498/1‑93 (MQ=255)

                                                                                       ggggCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGa  <  1:3034284/93‑1 (MQ=255)

                                                                                       ggggCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGa  <  2:3050943/93‑1 (MQ=255)

                                                                                       ggggCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGa  <  1:1782378/93‑1 (MQ=255)

CGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGA  >  NC_000913/4582771‑4582950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`