BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,078	C→T	W135* (TGG→TAG)	yjjP ←	putative structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,078	0	C	T	100.0%	831.5 / NA	229	W135* (TGG→TAG)	yjjP	putative structural protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (119/110); total (119/110)

Only 100 of 231 total aligned reads displayed.

GCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG  >  NC_000913/4599988‑4600167

 cAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATc                                                                                        >  1:1176420/1‑93 (MQ=255)

  aaaGAAGGTGATGACGGCACCATCCCAGCCGCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTGCCAGCTATCt                                                                                       <  1:2496476/93‑1 (MQ=255)

  aaaGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCt                                                                                       <  2:177040/93‑1 (MQ=255)

   aagaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                      <  1:3456207/93‑1 (MQ=255)

   aagaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                      >  2:2628913/1‑93 (MQ=255)

   aagaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCtt                                                                                      >  2:1870388/1‑93 (MQ=255)

     gaagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTgg                                                                                    <  2:2765740/93‑1 (MQ=255)

      aagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggg                                                                                   <  1:2113879/93‑1 (MQ=255)

       agGTGATGACAGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGt                                                                                  <  2:48355/93‑1 (MQ=255)

        ggTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTCTCTTgggga                                                                                 >  2:4244711/1‑91 (MQ=255)

        ggTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTgggca                                                                                 >  2:519685/1‑91 (MQ=255)

        ggTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTa                                                                                 <  2:383209/93‑1 (MQ=255)

        ggTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTa                                                                                 <  1:3170623/93‑1 (MQ=255)

          tgatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAc                                                                               <  1:3859190/93‑1 (MQ=255)

           gatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACg                                                                              >  1:189267/1‑93 (MQ=255)

           gatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACg                                                                              >  1:2753568/1‑93 (MQ=255)

               aCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAAt                                                                          <  1:4036621/93‑1 (MQ=255)

               aCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAAt                                                                          <  1:3152311/93‑1 (MQ=255)

                cGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATg                                                                         <  1:1345334/93‑1 (MQ=255)

                cGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATg                                                                         <  1:510840/93‑1 (MQ=255)

                 ggCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATgg                                                                        >  2:3653015/1‑93 (MQ=255)

                  gCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGc                                                                       >  1:2018588/1‑93 (MQ=255)

                  gCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGc                                                                       >  2:1136808/1‑93 (MQ=255)

                    aCCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGTCGCAAGAAAGGCCAACCATTAAGTCTACCAGCTATCTTGGGTAACGTAATGGCTg                                                                     <  1:173017/93‑1 (MQ=255)

                        tCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAtt                                                                 >  2:707432/1‑93 (MQ=255)

                          ccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTg                                                               >  1:3101573/1‑93 (MQ=255)

                            aGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGc                                                             <  2:1144900/93‑1 (MQ=255)

                            aGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGc                                                             >  2:4543884/1‑93 (MQ=255)

                            aGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAAGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATAGCTTAATTTggt                                                             >  2:2201934/1‑92 (MQ=255)

                             gCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCt                                                            <  2:2562727/93‑1 (MQ=255)

                             gCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCt                                                            >  2:4495170/1‑93 (MQ=255)

                                aCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                         <  1:4478792/93‑1 (MQ=255)

                                aCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                         >  1:1683920/1‑93 (MQ=255)

                                aCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                         <  2:3759514/93‑1 (MQ=255)

                                 ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  2:3034945/93‑1 (MQ=255)

                                 ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  1:4366026/93‑1 (MQ=255)

                                 ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        <  1:4633120/93‑1 (MQ=255)

                                  cGTTATTGAGTTTACAGAATGAGGCGCAAGAAGGGCCACCCATTAAGGATACAAGCTATCTTGGGTAACGTGGTGGCGGAATTTGGCTaaacc                                                       <  2:739905/93‑3 (MQ=255)

                                   gTTATTGAGTTTACAGAAACAGGCGCAAGAAGGGCCAACCATTAAGACTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCg                                                      <  2:4350921/93‑1 (MQ=255)

                                   gTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCg                                                      >  1:3697104/1‑93 (MQ=255)

                                   gTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCg                                                      >  2:785913/1‑93 (MQ=255)

                                    ttATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGt                                                     >  2:3019869/1‑93 (MQ=255)

                                     tATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGtt                                                    >  1:2585742/1‑93 (MQ=255)

                                     tATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGtt                                                    >  2:2279225/1‑93 (MQ=255)

                                      aTTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGttt                                                   >  2:3421900/1‑93 (MQ=255)

                                       ttGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtc                                                  <  1:2307559/93‑1 (MQ=255)

                                         gAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtctc                                                >  2:1590967/1‑93 (MQ=255)

                                         gAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtctc                                                >  2:431575/1‑93 (MQ=255)

                                           gTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTa                                              <  2:3494803/93‑1 (MQ=255)

                                           gTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTa                                              <  1:3263512/93‑1 (MQ=255)

                                           gTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTa                                              >  1:2810791/1‑93 (MQ=255)

                                           gTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTa                                              >  1:3825984/1‑93 (MQ=255)

                                            tttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAc                                             >  1:2833177/1‑93 (MQ=255)

                                                cAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCt                                         <  2:2847351/93‑1 (MQ=255)

                                                 aGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCtt                                        <  1:780012/93‑1 (MQ=255)

                                                  gAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCttt                                       <  2:3279614/93‑1 (MQ=255)

                                                     aCAGGCGCAGGAAAGGCCAACCATTAAGGCTACCAGCTACCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTa                                    >  2:1366527/1‑93 (MQ=255)

                                                      cAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTaa                                   >  1:4276271/1‑93 (MQ=255)

                                                      cAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTaa                                   <  1:735608/93‑1 (MQ=255)

                                                       aGGCGCAAGAGAGTCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAAt                                  <  2:2109125/93‑1 (MQ=255)

                                                          cgcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCca                               <  1:2347974/93‑1 (MQ=255)

                                                           gcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcag                              >  2:3311880/1‑93 (MQ=255)

                                                            cAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGCTTCTCTACGCCTTTGTAATCcagc                             >  2:2094755/1‑93 (MQ=255)

                                                             aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                            <  2:247652/93‑1 (MQ=255)

                                                             aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                            >  1:1309672/1‑93 (MQ=255)

                                                             aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                            >  2:884420/1‑93 (MQ=255)

                                                             aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                            >  1:1620306/1‑93 (MQ=255)

                                                              aGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagcag                           >  1:1289738/1‑93 (MQ=255)

                                                              aGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagcag                           <  1:4134238/93‑1 (MQ=255)

                                                              aGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagcag                           >  2:1727389/1‑93 (MQ=255)

                                                               gAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAga                          <  2:3641952/93‑1 (MQ=255)

                                                                aaaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgat                         >  2:3320571/1‑93 (MQ=255)

                                                                 aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatg                        >  1:485141/1‑93 (MQ=255)

                                                                 aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatg                        >  2:1756447/1‑93 (MQ=255)

                                                                  aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       <  2:615108/93‑1 (MQ=255)

                                                                  aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       <  2:3128534/93‑1 (MQ=255)

                                                                  aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       <  1:860746/93‑1 (MQ=255)

                                                                  aGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatga                       <  1:2268175/93‑1 (MQ=255)

                                                                   ggCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatgat                      >  1:1438994/1‑93 (MQ=255)

                                                                    gCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGatgatg                     <  1:2150565/93‑1 (MQ=255)

                                                                     ccAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGc                    >  1:91236/1‑93 (MQ=255)

                                                                      cAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCt                   <  1:3574375/93‑1 (MQ=255)

                                                                        aCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                 >  2:941636/1‑93 (MQ=255)

                                                                        aCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                 >  1:3364994/1‑93 (MQ=255)

                                                                         acATTAAGGCTACCAGCTATCTTGGGCAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCg                >  2:4214301/2‑93 (MQ=255)

                                                                          cATTAAGGCTACCAGCTATCTTGGGTCACGTAATGGCTGAATTTGGCTAAATCGCTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGc               >  2:2778473/1‑93 (MQ=255)

                                                                          cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGc               <  1:1555457/93‑1 (MQ=255)

                                                                           aTTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCa              >  1:2904322/1‑93 (MQ=255)

                                                                            ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGaaa             >  2:1407337/1‑93 (MQ=255)

                                                                            ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCaa             >  2:721950/1‑93 (MQ=255)

                                                                            ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCaa             >  2:4033263/1‑93 (MQ=255)

                                                                              aaGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGa           >  2:2348938/1‑93 (MQ=255)

                                                                               aGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGaa          <  2:4433973/93‑1 (MQ=255)

                                                                                ggCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGGCAGAAt         >  2:3473033/1‑93 (MQ=255)

                                                                                 gCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATc        <  2:2666524/93‑1 (MQ=255)

                                                                                     ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    <  1:661822/93‑1 (MQ=255)

                                                                                     ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    <  2:3530996/93‑1 (MQ=255)

                                                                                      cAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt   <  2:2924537/93‑1 (MQ=255)

                                                                                      cAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt   >  2:1472656/1‑93 (MQ=255)

                                                                                       aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg  <  1:2441044/93‑1 (MQ=255)

GCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG  >  NC_000913/4599988‑4600167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`