BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,735	T→C	intergenic (+3/‑54)	ybbX → / → ybbY	putative hydrolase/putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,735	0	T	C	100.0%	535.9 / NA	169	intergenic (+3/‑54)	ybbX/ybbY	putative hydrolase/putative transport
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (74/95); total (74/95)

Only 100 of 176 total aligned reads displayed.

TCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTA  >  NC_000913/539647‑539824

tCTTGCGGGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGcc                                                                                        >  1:1786072/1‑93 (MQ=255)

    gcGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTg                                                                                    >  2:2738565/2‑93 (MQ=255)

      gttgtgatgtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCTAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCa                                                                                  <  1:2711222/90‑1 (MQ=255)

      gtggtgATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCa                                                                                  >  1:4310124/1‑93 (MQ=255)

      gtggtgATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATCTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCa                                                                                  >  1:3421560/1‑93 (MQ=255)

        ggtgATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAAt                                                                                <  2:4126108/93‑1 (MQ=255)

        ggtgATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAAt                                                                                <  1:1916544/93‑1 (MQ=255)

            atgtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGccc                                                                            >  1:3538574/1‑93 (MQ=255)

              gtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGt                                                                          <  1:4639658/93‑1 (MQ=255)

                  tttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCtt                                                                      >  1:1863989/1‑93 (MQ=255)

                  tttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCtt                                                                      <  1:2978275/93‑1 (MQ=255)

                  tttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCtt                                                                      <  2:3495184/93‑1 (MQ=255)

                  tttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCtt                                                                      >  1:1165532/1‑93 (MQ=255)

                    tACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATACGGCCCCTGCAATGCCCGGCCTTgc                                                                    >  2:3210028/1‑93 (MQ=255)

                       gACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTgcgg‑c                                                                <  1:3997605/93‑1 (MQ=255)

                         cATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGcgg‑cgg                                                              <  1:2603182/93‑1 (MQ=255)

                            tGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCa                                                           <  1:815650/93‑1 (MQ=255)

                             gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCAt                                                          <  2:1818626/93‑1 (MQ=255)

                               aCAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATtc                                                        <  1:3678601/93‑1 (MQ=255)

                                cAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATtct                                                       <  2:1036723/93‑1 (MQ=255)

                                 aGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATtctc                                                      <  1:4635921/93‑1 (MQ=255)

                                 aGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATtctc                                                      <  2:2983958/93‑1 (MQ=255)

                                 aGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATtctc                                                      <  2:687640/93‑1 (MQ=255)

                                  gggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGGCTTCTcc                                                     >  1:2675132/1‑93 (MQ=255)

                                  gggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTcc                                                     >  1:3724947/1‑93 (MQ=255)

                                  gggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTcc                                                     >  1:3161405/1‑93 (MQ=255)

                                  gggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTcc                                                     <  2:1023408/93‑1 (MQ=255)

                                  gggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTcc                                                     >  1:1907699/1‑93 (MQ=255)

                                  gggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTT‑AACATCAGCAGGAATTCCGGCCCTGCAATGCCCCTCCCTGCGGCCGGGCTtcctcc                                                     >  1:3027924/1‑93 (MQ=255)

                                   ggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCg                                                    <  1:1815036/93‑1 (MQ=255)

                                   ggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCg                                                    >  2:3626925/1‑93 (MQ=255)

                                    gCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCgg                                                   >  1:3845192/1‑93 (MQ=255)

                                     cTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGt                                                  <  2:840219/93‑1 (MQ=255)

                                      ttCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGGACTTTCCgggt                                                 >  2:1620214/1‑91 (MQ=255)

                                      ttCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGtt                                                 <  2:2115388/93‑1 (MQ=255)

                                       tCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTa                                                >  1:373357/1‑93 (MQ=255)

                                       tCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTa                                                <  1:1968688/93‑1 (MQ=255)

                                        cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTaa                                               <  1:847469/93‑1 (MQ=255)

                                        cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTaa                                               <  2:493508/93‑1 (MQ=255)

                                        cccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTaa                                               <  1:288946/93‑1 (MQ=255)

                                         ccTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGGAATCTCCCGGTAAg                                              >  2:1720423/1‑93 (MQ=255)

                                          cTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAgg                                             <  1:3969348/93‑1 (MQ=255)

                                          cTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAgg                                             <  1:4043371/93‑1 (MQ=255)

                                          cTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGGTAAgg                                             >  1:3715252/1‑93 (MQ=255)

                                           tGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGgt                                            <  1:1568252/93‑1 (MQ=255)

                                            gTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGgtg                                           <  2:1294392/93‑1 (MQ=255)

                                            gTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAACgtg                                           >  1:4341906/1‑93 (MQ=255)

                                             ttGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGgtgt                                          <  2:1842706/93‑1 (MQ=255)

                                              tGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGgtgtg                                         <  1:4379275/93‑1 (MQ=255)

                                                  ccGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTa                                     <  2:2610305/93‑1 (MQ=255)

                                                    gAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTTAGGGGTGTTTAtt                                   >  2:3576258/1‑92 (MQ=255)

                                                     aaaGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGt                                  >  2:2334473/1‑93 (MQ=255)

                                                     aaaGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGGTAAGGGGTGTTTATGt                                  >  1:1477150/1‑93 (MQ=255)

                                                      aaGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGtt                                 <  2:1646504/93‑1 (MQ=255)

                                                      aaGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGtt                                 >  1:1358383/1‑93 (MQ=255)

                                                       aGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGGTAAGGTGTGTTTATGttt                                >  2:799377/1‑92 (MQ=255)

                                                       aGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGGTAAGGTGTGTTTATGTTc                                >  1:894127/1‑93 (MQ=255)

                                                         gTCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCaa                              >  1:2113835/1‑93 (MQ=255)

                                                          tCAATTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAAt                             >  1:3589722/1‑93 (MQ=255)

                                                            aaTTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGGATTCTCCGGTTAAGGTGTGTTTATGTTCAAttt                           >  2:2873597/1‑93 (MQ=255)

                                                            aaTTTATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAAttt                           <  2:140623/93‑1 (MQ=255)

                                                              tttATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTg                         <  1:3022950/93‑1 (MQ=255)

                                                              tttATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTg                         <  2:2705649/93‑1 (MQ=255)

                                                              tttATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTg                         <  2:2047253/93‑1 (MQ=255)

                                                              tttATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCCGTTTAGGTGTGTTTATGTTCAATTTTg                         >  1:54172/1‑93 (MQ=255)

                                                              tttATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCCGGTAAGGTGTGGTTATGTTCAAttttt                         >  2:2496212/1‑92 (MQ=255)

                                                               ttATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCCGTTTAGGTGTGTTTATGTTTAATTTTGc                        >  2:4037710/1‑93 (MQ=255)

                                                                tATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCa                       <  1:2618729/93‑1 (MQ=255)

                                                                tATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCa                       <  1:3606566/93‑1 (MQ=255)

                                                                tATCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCa                       <  1:1131559/93‑1 (MQ=255)

                                                                 aTCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAg                      <  2:2253017/93‑1 (MQ=255)

                                                                  tCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTTCAGt                     >  2:851700/1‑93 (MQ=255)

                                                                  tCCTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCAATCTCCCGGTTAGGTGTGTTTATGTTCAATTTTGCAGt                     >  1:2033551/1‑93 (MQ=255)

                                                                    cTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCa                   >  1:4364380/1‑93 (MQ=255)

                                                                    cTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCa                   <  2:773848/93‑1 (MQ=255)

                                                                    cTTAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATGCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCa                   <  2:715553/93‑1 (MQ=255)

                                                                      tAAACATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGc                 <  2:4533197/93‑1 (MQ=255)

                                                                       aaaCATCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGcc                <  2:430573/93‑1 (MQ=255)

                                                                           aTCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGa            <  1:4405027/93‑1 (MQ=255)

                                                                            tCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaa           >  2:1516178/1‑93 (MQ=255)

                                                                            tCAGCAGTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGGTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaa           >  2:4470229/1‑93 (MQ=255)

                                                                             cagcagTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaaa          <  1:2705914/93‑1 (MQ=255)

                                                                             cagcagTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaaa          >  1:2938720/1‑93 (MQ=255)

                                                                               gcagTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGc        >  1:1528008/1‑93 (MQ=255)

                                                                                cagTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGcc       <  2:3321972/93‑1 (MQ=255)

                                                                                cagTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGcc       <  2:3259456/93‑1 (MQ=255)

                                                                                cagTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGcc       <  2:2965669/93‑1 (MQ=255)

                                                                                 cgTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCt      <  1:4680910/92‑1 (MQ=255)

                                                                                 agTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTTCAGTCAGCCGCGAAAGCCt      >  1:2192001/1‑93 (MQ=255)

                                                                                 agTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCt      <  1:4225846/93‑1 (MQ=255)

                                                                                 agTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCt      >  1:2043386/1‑93 (MQ=255)

                                                                                 agTAATCCGCCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCt      <  2:3707623/93‑1 (MQ=255)

                                                                                  gTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTTCGGTTAAGGTGTGTTTATGTTTAATTTTGCAGGCAGCCGCGAAAGcccg     >  1:1397800/1‑91 (MQ=255)

                                                                                  gTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGCAAGACTg     >  1:2916113/1‑93 (MQ=255)

                                                                                  gTAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTg     <  1:2639719/93‑1 (MQ=255)

                                                                                  gTAATCCGGCCCCTGCAATGCCCGACCTTGCGG‑CGGGCATTCTTCGGTTCAGGGGTGTTTATGGTCAACTTTGCAGTTAGGAGCGGAAGGCTg     >  2:1931464/1‑93 (MQ=255)

                                                                                   tAATCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGCGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGt    <  2:1052393/93‑1 (MQ=255)

                                                                                    aaTCCGGCCCCTGCGATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCACACAGCCGCGAAAGCCTGtt   <  2:3424455/93‑1 (MQ=255)

                                                                                     aTCCGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTa  >  1:1767400/1‑93 (MQ=255)

                                                                                     aTCCGGCCCCTGCAACGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTa  >  1:1429604/1‑93 (MQ=255)

TCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGG‑CGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTA  >  NC_000913/539647‑539824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`